• Title/Summary/Keyword: Setae

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Optimization of in Vitro Cultivation of Inonotus Obliquus

  • Cho, Nam-Seok;Shin, Yu-Soo
    • Journal of the Korean Wood Science and Technology
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    • v.33 no.5 s.133
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    • pp.92-98
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    • 2005
  • This study was performed to get the basic information concerned to the optimum culture condition of Inonotus obliquus. Several solid media, PDA, MEA and Czapek-Dox, and three liquid media were adopted for the in vitro cultivation. Some main features of the fungal morphological characteristics under cultivation conditions were observed and described. Preliminary results showed that appearance of the mycelial mat, hyphal size and substrate pigmentation differed according to the media. The PDA medium was the most favorable substrate for the growth on solid culture, followed by MEA and Czapek-Dox media. Concerned to the addition of amino acids, 5 amino acids, such as alanine, alginine, isoleucine, leucine and threonine, enhanced to the mycelial growth. Isoleucine was shown the best fungal growth. An important morphological hyphal structure for the fungus, the setae, was found in abundance and diverse its shape and size. In liquid culture, fresh potato broth was the best growth stimulant of the fungus, followed by Malt extract and potato broth. Addition of yeast extract to the liquid media had improved the biomass, but not laccase production.

A New Species of Chigger Mite (Acari: Trombiculidae) from Rodents in Southwest China

  • Ren, Tian-Guang;Guo, Xian-Guo;Jin, Dao-Chao;Wu, Dian;Fletcher, Quinn E.
    • Parasites, Hosts and Diseases
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    • v.52 no.1
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    • pp.63-67
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    • 2014
  • This paper describes a new species of chigger mite (Acari: Trombiculidae), Gahrliepia cangshanensis n. sp., from rodents in southwest China. The specimens were collected from Yunnan red-backed voles, Eothenomys miletus (Thomas, 1914), and a Chinese white-bellied rat, Niviventer confucianus (Milne-Edwards, 1871) in Yunnan Province. The new species is unique mainly in its number of dorsal setae (n=21), and it has the following features: fT (formula of palpotarsus)=4B (B=branched), fp (formula of palpal seta)=B/N/N/N/B (N=naked), a broad tongue-shaped scutum with an almost straight posterior margin, and 17 PPLs (posterior posterolateral seta) with a length of 36-43 ${\mu}m$. This chigger mite may also infect other rodent hosts and may be distributed in other localities.

Isolation and Identification of Colletorichum musae from Imported Bananas

  • Lim, Jin-Young;Lim, Tae-Heon;Cha, Byeong-Jin
    • The Plant Pathology Journal
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    • v.18 no.3
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    • pp.161-164
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    • 2002
  • Colletotrichum musae was isolated from dark-brown anthracnose lesions on commercial banana (Musa sapientum L.) to establish the causal agent of the symptom. The fungus grew fast and produced white aerial mycelium on PDA. Acervuli developed abundantly on culture plates after incubation for 10 days at $25^{\circ}C$. Pinkish conidial masses were produced on the acervuli, which mostly coalesced together, Conidia were aseptate, hyaline, straight, ellipsoid to globose, and 14.5$\times$6.9 $\mu\textrm{m}$ in size. Black, clavate, round, or irregular-shaped appressoria measuring 8.8$\times$6.8 $\mu\textrm{m}$ were readily formed from germ tubes. Setae-like structures were not found either on the lesion or on the cultures. Sclerotia were also absent. Among the media, PDA medium was the best for mycelial growth. The optimum temperature for mycelial growth was $28^{\circ}C$, while the optimum pH ranged from pH 5.5 to 6.5. The isolates of C musae caused black necrotic lesions on banana fruits by needle-wound inoculation, and orange-colored spore masses were produced on the lesions. The fungus also caused discoloration on apple fruits inoculated.

Two New Species of Clausidiidae (Copepoda, Poecilostomatoida) from Korea

  • Hwang, Hosung;Lee, Jimin;Kim, Il-Hoi
    • Animal Systematics, Evolution and Diversity
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    • v.32 no.2
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    • pp.93-104
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    • 2016
  • Two new species of Clausidiidae, each belonging to the genera Clausidium Kossmann, 1875 and Hippomolgus G. O. Sars, 1917, are described from Korea. Clausidium maximus n. sp. is an associate of a burrowing decapod of the genus Callianassa living on the Korean coast of the Yellow Sea and has, as diagnostic characters, two inner setae on the second endopodal segment of legs 2-4, nine elements on the third exopodal segment of leg 4, an inner seta on the second exopodal segment of male leg 1, and a relatively large body size, exceeding 2.0 mm in the female. Hippomolgus limiticus n. sp. was found in the bottom sediments in the East China Sea. It is similar to H. furcifer G. O. Sars, but distinghuishable from the latter species by having shorter caudal rami, 4-segmented female maxilliped, and inner coxal spine (instead of seta) on legs 2 and 3. This is the first record on the genera Clausidium and Hippomolgus in the West Pacific.

Fine Structure of the Mouthparts in the Ambrosia Beetle Platypus koryoensis(Coleoptera: Curculionidae: Platypodinae)

  • Moon, Myung-Jin;Park, Jong-Gu;Kim, Kyung-Hee
    • Animal cells and systems
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    • v.12 no.2
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    • pp.101-108
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    • 2008
  • Recently, Platypus koryoensis has been reported as a major pest of oak trees in Korea which can introduce the pathogenic fungus(Raffaelea sp.) by making galleries into the heartwood of the tree. As the beetle has developed its effective drilling mouthpart enough to make tunnels, we have analyzed the fine structural aspects of the mouthpart using the field emission scanning electron microscopy(FESEM) to reveal its mechano-dynamic characteristics. The mouthparts of this ambrosia beetle which consist of a labrum, a pair of mandibles, a pair of maxillae and the labium exhibit typical morphology of mycophagous coleopteran beetles and have those characteristics of chewing mouthparts that can excavate galleries in the hardwood. Both of maxillary and labial palpi have the functions of direct the food to the mouth and hold it while the mandibles chew the food. The distal ends of these palpi are flattened and have shovel-like setae. Females have larger maxillary palpi and a larger gular region than males in general.

External Ultrastructure of the Adult Genital Segments of Pine Needle Gall Midge, Thecodiplosis japonensis Uchida et Inouye (솔잎혹파리(Thecodiplosis japonensis Uchida et Inouye)에 관한 연구 II - 성충 생식절의 표면미색구조 -)

  • 한성식;박용철;이찬용
    • Korean journal of applied entomology
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    • v.27 no.3
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    • pp.144-148
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    • 1988
  • Using scanning electron microscope, we studied the adult genital segments of pine needle gall midge, Thecodiplosis Japonensis, Uchida et Inouye Male: There were two pairs of hypoproct a pair of claspettes and aedeagus, Hypoproct were surrounded with many setae and sensilla. Clasperres which was projected below hypoproct was composed of two aegments and had many sensilla Aedeagus was long and flattened and possesed some sensilla at the terminal. Female :Ovipositor was segmented into three parts. Each segment had a different externla sturcture; particularly, the 2nd one was covered with reversed boat-like attachments and did not show any sensillum.

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Unrecorded subgenus of black fly (Diptera: Simuliidae) from Korea

  • Kim, Sam-Kyu
    • Journal of Species Research
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    • v.9 no.1
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    • pp.19-25
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    • 2020
  • Larvae, pupae, and adults reared from the pupae of Simulium (Boreosimulium) konoi were collected and recorded from Korea for the first time. I provide detailed descriptions of both immatures and adults accompanied with photographs. The subgenus Boreosimulium, previously unknown from Korea, contains 19 species in 3 species-groups worldwide and only 2 species, viz, S. (B.) konoi and S. (B.) tokachiense, are recorded from Japan. Simulium konoi is unique among the members of the subgenus by having water dropshaped denticles on the anterior portion of the cibarium in females, which is considered as an autapomorphic character so far not found in other black flies, W or inverted V-shaped ventral plate of male genitalia, and larval thorax and abdominal segments covered with short black setae. Korean specimens of S. konoi collected from two adjacent localities can readily be recognized by denticles on the cibarium in females and well-developed ventral plate of male genitalia but showed geographical variation, e.g., lacking ventral tubercle on larval abdominal segment IX. Due to the autapomorphy and other characters found in S. konoi, the current placement of the S. konoi in the subgenus Boreosimulium or species-group assignment is tentative.

Effects of Antibiotics, Fenbendazole and Lincomycin, in Benthic Copepod, Tigriopus japonicus s.l. (저서성 요각류 Tigriopus japonicus s.l.에서 항생제 Fenbendazole과 Lincomycin의 영향)

  • Lee, Dong-Ju;Kwak, Inn-Sil;Bang, Hyun-Woo;Lee, Won-Choel
    • Environmental Analysis Health and Toxicology
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    • v.25 no.3
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    • pp.197-205
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    • 2010
  • The ecotoxicological effects of two antibiotics, fenbendazole and lincomycin, were observed in the harpacticoid copepod Tigriopus japonicus s.l. collected from tidal pools in the southern coast of Korea. Fenbendazole had a significant effect on the survival rates (p < 0.05), delay of copepodite emergence, and urosome size (p < 0.05). Lincomycin, on the other hand, had no significant influence on these environmental indicators. However, our analysis of morphological abnormalities in T. japonicus s.l. showed that lincomycin was more effective than fenbendazole in causing deformities. The pattern of deformity was diverse, with fused segments, and loss or addition of setae in the swimming legs. All of these patterns appeared as a result of relatively low concentrations of this antibiotic (0.3, $1\;{\mu}g\;L^{-1}$). We report here patterns of morphological abnormality in T. japonicus s.l. exposed to antibiotics, and suggest their possible application in ecotoxicological monitoring.

Sexual Dimorphism on the Genus Paracalanus(Copepoda: Paracalanidae) in Korean Waters

  • Cho, Kyu Hee;Lee, Won Choel;Kim, Saywa
    • Proceedings of the Korea Society of Environmental Biology Conference
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    • 2003.11a
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    • pp.116-120
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    • 2003
  • The genus Paracalanus Boeck, 1864 dominate zooplankton communities in waters off Korean peninsula. Zooplankton samples collected from four waters (Uljin, Hadong, Youngkwang, Cheju) were sorted for specimens belonging to the genus Paracalanus. Paracalanus sp. regarded to p. paMus or p. indicus were examined. Body of male has cephalosome dorsal hump (CDH). Urosomites have four segments in female and five segments in male. In male, antennule is symmetry and fused without geniculation. The male antennule carries more aesthetascs than those in female antennule. Female P1-P4 is similar to those of male in seta and spine formular. Spinules on surface of legs are different each other. The female P5 is symmetrical, and composed of 2 segment. In male, it is aymmetrical and left leg composed of 5 segments, right leg 2-segmented. Male found patch of fine spinule on terminal inner surface of outer process on fifth segment. Sexual dimorphism appear saliently in mouth appendages. Mandible lacks of gnathobase; maxillule is apparently reduced; maxilla degrades it i]l female and remains vestiges between maxillule and maxilliped; maxilliped terminal part indistinctly articulated and carrying behind three strong plumose setae. With the absence of gnathobase in male, we conclude that male Paracalanus sp. does not feed.

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Two species of copepods (Crustacea) parasitic on marine fish, Konosirus punctatus, from Kamak Bay in Korea (한국산 어류 (전어)에 기생하는 요각류 2종 (Nothobomolochus thambus, Mitrapus heteropdus))

  • Choi, Sang-Duk;Hong, Sung-Yun;Rho, Yong-Gil
    • Journal of fish pathology
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    • v.8 no.1
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    • pp.1-11
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    • 1995
  • Two species of the parasitic copepods, Nothobomolochus thambus (Poecilostomatoida, Bomolochidae) and Mitrapus heteropodus (Siphonostomatoida, Lernanthropidae), were recovered on the gills of a marine fish, Konosirus punctatus (Temminck and Schlegel) taken from Kamak Bay in Korea. N. thambus is very distinguishable in the armature of maxilliped from all other species of Nothobomolochus; one of the two strong, hairy setae has become naked. M. heteropodus is very distinguishable in the armature of leg 4; the inner process (endopod) is only about one - fifth the length of the outer process (exopod), Both parasitic copepod species are new to the Korean fauna.

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