• 한국식품영양과학회지
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• 제21권6호
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• pp.627-632
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• 1992

식이 중의 펙틴이 식이의 상부소화관내 이동 속도에 미치는 영향을 조사하였다. 흰쥐에게 무섬유식이 또는 10% 펙틴식이를 공급하면서 식이의 이동속도 표시물질인 51-Cr를 투여하였다. 51-Cr 투여후 20분부터 6시간까지 정해진 시간 간격을 두고 동물을 희생시켜, 51-Cr이 소화관에서 이동하는 속도를 측정하였다. 51-Cr를 섭취한 뒤 3시간까지 펙틴은 51-Cr이 위에서 소장으로 이동해 나가는 속도를 유의성있게 증가시켰다. 51-Cr을 섭취한 후 3시간과 4시간 사이에 펙틴은 51-Cr이 소장을 통과하여 맹장으로 이동해 나가는 속도를 증가시켰다. 따라서 펙틴이 포도당 부하후 또는 식이섭취후 혈당치와 인슐린치의 급격한 증가를 완화시키는 효과가 펙틴이 상부소화관내 이동속도를 지연시키기 때문이라는 가설은 정확하지 않은 것으로 보인다.

• Journal of Pharmaceutical Investigation
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• 제25권3호
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• pp.185-192
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• 1995

Precipitation reaction occured between berberine in Coptidis Rhizoma and glycyrrhizin in Glycyrrhizae Radix when they were boiled together in aqueous solution and the supernatant solution thus obtained did not show any antibacterial activity which was derived from berberine. The content of berberine in BG and CGP by HPLC analysis were 41.1%, 8.3% respectively. BG was occured mostly at pH 5.0. The solubility of berberine was 0.15%, while that of BG and CGP was 0.07%, 0.12%, respectively. CGP shown more increased antibacterial activity to gram positive bacteria, S. dysenteriae and K. pneumoniae than berberine. The absorption rates of CGP in stomach, duodenum and jejunum of rats were compared with those of Coptidis Rhizoma water extracts (CR), which were increased more than CR. The time required for the maximum serum concentration of berberine from CGP in mice was 90 minutes after oral administration. The maximum serum concentration of berberine from CGP was higher than that from CR. The dissolution of CGP was increased more than berberine and BG in both artificial gastric and intestinal fluids. The dissolution of CGP pill made from gelatin was 63.4% in artificial gastric fluids and that made from CMC was 76.0% in artificial intestinal fluids.

• Asian-Australasian Journal of Animal Sciences
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• 제24권12호
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• pp.1674-1680
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• 2011

This study was conducted to examine the effects of glucose, chromium picolinate (CrP), and vitamin C (Vit C) on lipid metabolism in Korean native steers fitted with indwelling catheters. A total of 12 Korean native steers were randomly allocated to the following treatments: 1) normal control diet, 2) same as 1) +250 g of glucose by intravenous (IV) infusion, 3) same as 2)+13.5 g CrP administered orally, and 4) same as 3)+2.52 g Vit C by IV infusion. Glucose, Vit C, and CrP treatments were administered for five days. At days 1 and 3, serum insulin was higher in treated animals than in those fed the control diet (p<0.05). Serum non-esterified fatty acid (NEFA) concentration in the steers on treatment 2), control+13.5 g CrP, was lower than those on other treatments at 90 min post-infusion on days 1 and 3 (p<0.05). The expression of peroxisome proliferator-activated receptor-${\gamma}$ (PPAR${\gamma}$)2, stearoyl-CoA desaturase-1 (SCD), fatty acid synthase (FAS), and glucose transporter type 4 (Glut 4) in the longissimus muscle of steers on treatment 2 was higher than those on other treatments. In conclusion, the results suggest that CrP is associated with the regulation of gene expression involved in adipogenesis.

• Clinical and Experimental Pediatrics
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• 제63권4호
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• pp.151-156
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• 2020

Background: Acute kidney injury (AKI) is one of the most significant postoperative complications of pediatric cardiac surgery. Because serum creatinine has limitations as a diagnostic marker of AKI, new biomarkers including neutrophil gelatinase-associated lipocalin (NGAL), kidney injury molecule-1 (KIM-1), and interleukin-18 (IL-18) are being evaluated to overcome these limitations and detect AKI at an early stage after cardiac surgery. Purpose: This study aimed to investigate the clinical usefulness of these biomarkers in young children. Methods: Thirty patients with congenital heart diseases who underwent cardiac surgery using cardiopulmonary bypass (CPB) were selected, and their urine and blood samples were collected at baseline and 6, 24, and 48 hours after surgery. Serum creatinine and blood urea nitrogen levels as well as NGAL, KIM-1, and IL-18 levels in urine samples were measured, and clinical parameters were evaluated. Results: Of the 30 patients, 12 developed AKI within 48 hours after cardiac surgery. In the AKI group, 8 of 12 (66.6%) met AKI criteria after 24 hours, and urine KIM-1/creatinine (Cr) level (with adjustment of urine creatinine) peaked at 24 hours with significant difference from baseline level. Additionally, urine KIM-1/Cr level in the AKI group was significantly higher than in the non-AKI group at 6 hours. However, urine NGAL/Cr and IL-18/Cr levels showed no specific trend with time for 48 hours after cardiac surgery. Conclusion: It is suggested that urine KIM-1/Cr concentration could be considered a good biomarker for early AKI prediction after open cardiac surgery using CPB in young children with congenital heart diseases.

• 한국동물생명공학회지
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• 제38권3호
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• pp.143-150
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• 2023

Background: The successful production of superior or transgenic offspring from in vitro produced embryos in cattle relies heavily on the quality of blastocyst stage embryos. In order to enhance the developmental competency of these embryos, a novel culture method was devised. Methods: This study utilized stem cell culture medium (SCM) from hESCs as a supplement within the culture medium for bovine in vitro produced embryos. To gauge the efficacy of this approach, in vitro fertilized embryos were subjected to culture in CR1aa medium enriched with one of three supplements: 0.3% BSA, 10% FBS, or 10% SCM. Results: The blastocyst development and hatching rates of one-cell zygotes cultured in CR1aa medium supplemented with SCM (23.9% and 10.2%) surpassed those cultured in CR1aa medium supplemented with BSA (9.3% and 0.0%) or FBS (3.1% and 0.0%) (p < 0.05). Furthermore, post-zygotic gene activation, cleaved embryos cultured in CR1aa medium supplemented with SCM (57.8% and 34.5%) exhibited notably higher rates (p < 0.05) compared to those cultured with BSA (12.9% and 0.0%) or FBS (45.7% and 22.5%) supplementation. Furthermore, the microinjection of SCM into the cytoplasm or pronucleus of fertilized zygotes resulted in elevated blastocyst development and hatching rates, particularly when the microinjected embryos were subsequently cultured in CR1aa medium supplemented with SCM from the 8-cell embryo stage onwards (p < 0.05), in contrast to those cultured with FBS supplementation. Conclusions: In conclusion, this study conclusively demonstrated that the incorporation of SCM into the culture medium significantly enhances the developmental progress of preimplantation embryos.

• 분석과학
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• 제15권6호
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• pp.509-513
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• 2002

본 연구는 우리나라 성인여성의 혈청 내 미량 무기 원소의 농도를 조사하기 위해 중성자 방사화 분석법을 적용하였다. 혈청시료중의 나트륨과 염소의 높은 농도가 분석에 제헌을 주었으며 이들의 간섭을 최소화시킨 분석조건에서 Br, Ca, Cl, Co, Cr, Cs, Fe, K, Na, Rb, Se, Zn 와 같은 12종 원소의 분석이 가능하였다. 63명의 성인여성으로부터 채취한 혈청시료를 분석시료를 분석하여 각 원소의 농도수준과 범위 등을 결정하였으며 미국표준연구소의 표준물질들을 혈청시료와 동시에 분석하여 분석품질관리에 사용하였다. 혈청시료중의 무기질원소들의 평균값은 Na 과 Cl은 약 3000 mg/L, Ca은 약 100 mg/L, K은 200 mg/L, Br, Se 및 Zn은 각각 6.0, 0.1, 1.0 mg/L 등의 농도수준을 보였으며 지금까지 보고된 값과 비교한 결과 큰 차이를 보이지 않았다.

• Journal of Acupuncture Research
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• 제26권6호
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• pp.1-9
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• 2009

Objectives : For evaluation of preventive and anti-diabetic activities of Cinnamomi ramulus(CR) herbal acupuncture on pancreatic islet damage in streptozotocin(STZ)-induced diabetic rat. Methods : CR herbal acupuncture was performed at Bisu($BL_{20}$) for 3 weeks subcutaneously starting1 week before STZ i.p. injection. SD rats were divided into four groups(n=10 for each group); 1) NC group, non-treated normal control group, 2) STZ group, STZ administered control group, 3) CR125 group, CR(125mg/kg) + STZ administered group, and 4) CR250 group, CR(250mg/kg) + STZ administered group. Results : Both of CR250 and CR125 groups showed increase in insulin secretion and decrease in the level of serum triglyceride and non-esterified fatty acid in a dose-dependent manner compared to the STZ group. Only CR250 group showed decrease in the levels of glucose and total cholesterol compared to the STZ group. CR herbal acupuncture prevents $\beta$-cell damage of pancreatic islet, showing round figure on the sections of the pancreas. In the pancreatic cells, expressions of iNOS, JNK-2, P-JNK-1/2 and ERK-1/2 were decreased compared to the STZ group. CR herbal acupuncture solution did not show any cytotoxicity by MTS assay and inhibited expressions of iNOS and COX-2 in the STZ-induced diabetic rats. Conclusions : Therefore, we suggest that CR herbal acupuncture may act as a prophylactic as well as a therapeutic modality for diabetes mellitus.

• 한국식품영양과학회지
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• 제33권1호
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• pp.107-113
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• 2004

본 연구는 부천시에 거주하는 외견상 건강하면서 65세 이상 노인 138명 (남: 38명, 여: 100명)을 대상으로 하여 골밀도와 관련된 각 요인을 분석한 결과 다음과 같은 결론을 얻었다. 여자 노인은 남자 노인에 비해 칼슘, 비타민 C를 제외한 모든 영양소의 섭취량이 유의하게 낮았다(p<0.001∼p<0.05). 남녀 모두 칼슘과 비타민 D 섭취량은 각각 331.0 mg, 1.89 $\mu\textrm{g}$과 308.6 mg, 1.21 $\mu\textrm{g}$으로서 RDA의 50% 미만이었다. 남자노인의 경우 요추골밀도, 대퇴부 골밀도는 에너지 섭취량(p<0.05), 칼슘 섭취량(p<0.05), 비타민 D 섭취량(p<0.05)과 양의 상관관계를 보였다. 여자노인의 경우 요추골밀도, 대퇴부 골밀도는 에너지, 당질, 단백질, 지방, 칼슘, 비타민 D 섭취량 모두와 양의 상관관계를 보였다(p<0.01∼p<0.05). 여자 노인의 혈청 osteocalcin과 소변의 DPYR/CR값이 남자에 비해 유의하게 높아(각 p<0.01, p<0.001)남자 노인에 비해 골 교체율과 골 흡수가 높은 것을 알 수 있었다. 25(OH)VitD$_3$가 10 ng/mL미만으로서 비타민 D 결핍을 보인 노인이 남자 23.7%, 여자 35.0%였다. 혈청 25(OH)VitD$_3$는 척추, 대퇴 전자부 골밀도와 유의한 정의 상관관계 (p<0.05)를 보였으나 혈청 alkaline phosphatase, 소변의 DPYR/CR, 소변의 P/CR의 비는 대부분의 부위에서 골밀도와 음의 상관관계를 보였다(p<0.001∼p<0.05). 다단계 다중회귀분석을 실시한 결과 요추골밀도는 활동량, 혈청 ALP, 체중, 비타민 D 섭취량에 의해 골밀도 변이의 47.6%를 설명할 수 있었으며 그 중에서도 비타민 D 섭취량(p<0.001), 체중(p<0.001), 활동량(p<0.05)이 감소할수록 골밀도는 감소했고 혈청 ALP는 증가할수록 골밀도는 감소하였다(p<0.01). 대퇴전자부 골밀도는 연령, 혈청ALP, 체중에 의해 골밀도 변이 의 45.9%를 설명할 수 있었고 나이가 많을수록(p<0.01), 혈청 ALP가 높을수록(p<0.01), 체중이 적을수록(p<0.001) 골밀도가 낮았다.

• Journal of Preventive Medicine and Public Health
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• 제28권2호
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• pp.421-432
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• 1995

The influence of lead exposure on renal function was studied. Eighty nine lead exposed workers who worked in 2 storage battery factories, and seventy one control workers were chosen for this study. Blood lead(PbB) and zinc protoporphyrin in whole blood(ZPP) were selected as indicators of lead exposure. As indicators of renal function, urinary N-acetyl-$\beta$-D-glucosaminidase(NAG), blood urea nitrogen(BUN), serum creatinine(S-Cr), total protein in urine(U-TP),and serum uric acid(S-Ua) were selected. The results obtained were as follows: 1. While the mean values of lead exposure indicators of lead workers were significantly different from non-exposed ones, the mean values of NAG, U-TP, BUN and S-Cr of renal function indicators of exposed were also significantly different from non-exposed but their mean values were all within normal limits. 2. BUN, logarithmic U-TP, logarithmic NAG and S-Cr showed statistically significant correlation with PbB. 3. The proportion of workers whose values of renal function indicators were over the normal limits(NAG7.5 U/g Cr ; U-TP10.9 mg/dl ; BUN20 mg/dl ; S-Crl.2 mg/dl ; S-Ua7.0 mg/dl) by the level of lead absorption in terms of PbB and ZPP were calculated. The proportion of workers with over the normal limits of U-TP among total workers showed the dose-response relationship. When age is adjusted, U-TP showed significantly strong dose-response relationship with the level of PbB and ZPP.

• 대한핵의학회지
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• 제2권1호
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• pp.27-41
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• 1968

The ferrokinetics and red cell life spans of the patients with chronic glomerulonephritis were investigated by the double tracing method using radioactive iron ($^{59}Fe$) and chromium ($^{51}Cr$). According to the serum NPN levels, the patients were subdivided into 3 groups: Group 1. 6 patients, had the levels below 40 mg/dl Group 2. 6 patients, had the levels between 41 mg/dl to 80 mg/dl Group 3. 10 patients, had the levels above 80 mg/dl The results were as follows: 1) Red blood cell-, hematocrit- and hemoglobin values were moderately reduced in patients with normal serum NPN levels, while markedly reduced in patients with elevated serum NPN levels. 2) The plasma volume was increased, while the red cell volume was decreased in patients with elevated serum NPN levels, hence, total blood volume was unchanged. 3) The serum iron level was slightly reduced h patients of groups 1 and 2, while was within the normal ranges in patients of group 3. 4) i) In patients with normal serum NPN levels, the plasma iron disappearance rate, red cell iron utilization rate, red cell iron turnover rate, daily red cell iron renewal rate, circulating red cell iron and red cell iron concentration were within the normal ranges, while the plasma iron turnover rate was slightly reduced. ii) In patients with elevated serum NPN levels, the plasma iron disappearance rate was delayed, while the plasma iron turnover rate was within the normal ranges. The red cell iron utilization rate, red cell iron turnover rate and circulating red cell iron were decreased and the period in which the red cell iron utilization rate reachd its peak was delayed in Group 3 patients. The daily red cell iron renewal rate and the red cell iron concentration were unchanged. iii) The mean red cell life span was within the normal ranges in patients with normal serum NPN levels, while was shortened in patients with elevated serum NPN levels.