• Journal of Korean Neurosurgical Society
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• 제39권4호
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• pp.271-276
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• 2006

Objective : Despite the recent progress that has been made in intracerebral monitoring, it is still difficult to quantify the exact extent of primary brain damage after severe head injury. In this work, we investigate the role of S-100B protein as a serum marker of brain damage after severe head injury. Methods : 21 patients with severe head injury [GCS score <9] were selected for this prospective study. A venous blood sample was taken as soon as possible after head injury and the serum concentration of S-100B protein was measured daily for five consecutive days. The serum level of S-100B protein was compared with the patients' outcome. The outcome was measured twice, at hospital discharge and after 6 months of follow-up using the Glasgow Outcome Scale[GOS]. Results : Those patients who died within two weeks [after head injury] had a significantly higher serum S-100B value than those who survived [median, 9.64ug/L versus 2.91ug/L]. Seven [78%] of the nine patients who died had a maximum S-100B value of 2ug/L or higher, while three [25%] of the twelve surviving patients showed a maximum S-100B protein value of more than 2ug/L [P<005]. Conclusion : These results indicate that S-100B protein appears to be the most reliable index for estimating the extent of brain damage.

• Journal of Nutrition and Health
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• 제32권8호
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• pp.855-863
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• 1999

This study was performed to investigate the effect of dietary protein level throughout gestation and lactation on milk composition and on postnatal growth in infants, using rats as an animal model. Female Sprague-Dawley rats were provided with either high(25% ISP(Isolated Soy Protein)diet) or low protein diet(10% ISP diet) throughout gestation and lactation. Milk samples were taken for analysis from the lactating rats at days of 7, 14, 21, of lactation. Dams and some pups were killed after 4 weeks from parturtion (Experiment 1). Pups from dams of each diet groups were randomly selected and reared with 25% or 10% ISP diet for 4 more weeks (Experiment 2). In experiment 1, maternal protein intake and body weight gain throughout gestation and lactation was higher in 25% ISP group. Serum protein, Ca, Fe, Zn, K concentrations were significantly higher in 25% ISP group. There was no difference in birth weight between two groups, however the mean body weight at 4 weeks postpartum were significantly higher in 25% ISP group. Serum profiles of pups at weaning were similar to that of dams. Milk compositions were changed during lactation processes and were affected by dietary protein level. Lactose and Ca, Cu, Fe concentrations in milk were higher in 25% ISP group, whereas, lipid, triglyceride were higher in 10% ISP group. In experiment 2, food intake was higher in milk were higher in 25% ISP group but was unaffected by pup's dietary protein level after weaning. The weights of liver and kidney were affected by maternal protein intake. The weight of intestine was affected by pup's dietary protein level after weaning. The weight of femur and scapula were affected by maternal protein intake. There were no differences between four groups in serum profiles. Therefore, as mentioned above, it seemed that the effect of maternal protein malnutrition to fetus was able to be overcome to some extent by high protein diet intake after weaning. In conclusion, 1) Dietary protein level throughout gestation and lactation affected both nutritional status of dams and pups and milk composition: 25% ISP groups supported better nutritional status than 10% ISP group 2) It seemed that effect of dietary protein level after weaning on pups was able to be overcome the influence of maternal diet in fetus to some extent.

• 한국식품영양과학회지
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• 제12권4호
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• pp.368-373
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• 1983

제사공장(製絲工場)에서 부산물(副産物)로 나오는 번데기 단백질(蛋白質) 영양가(營養價)를 측정(測定)하기 위하여 이의 아미노산조성을 분석하였고 casein군(群)($D_1$), 대두단백질군(大豆蛋白質群)($D_2$), 대두단백질(大豆蛋白質)+20%번데기단백질군(蛋白質群)($D_3$), 대두단백질(大豆蛋白質)+40%번데기단백질군(蛋白質群)($D_4$), 번데기단백질군(蛋白質群)($D_5$)등(等) 5군(群)의 식이(食餌)로 나누어 동물실험(動物實驗)을 통(通)해 성장률(成長率), protein efficiency ratio, 장기중량(臟器重量), 그리고 hematology 및 total serum protein과 albumin의 함량을 측정(測定)하여 비교(比較)하였다. 번데기단백질(蛋白質)의 아미노산조성은 FAO의 provisional scoring pattern과 비교(比較)하였을 때 필수아미노산 조성이 우수하였으며 특히 lysine과 methionine의 함량이 높은 것은 곡류(穀類)와 두류(豆類) 단백질(蛋白質)에 부족(不足)한 필수아미노산을 보충(補充)하는데 좋은 단백질원(蛋白質源)으로 이용(利用)될 수 있다. 번데기단백질군(蛋白質群)의 체중증가량(體重增加量)과 단백효율(蛋白效率)은 대두단백질(大豆蛋白質)보다 우수하였으며 대두단백질(大豆蛋白質)에 첨가하는 양(量)의 증가(增加)에 따라 성장율과 단백효율(蛋白效率)이 증가(增加)하는 경향을 나타내었다. liver와 spleen의 총 중량은 대두단백질군(大豆蛋白質群)에 비(比)해 번데기단백질(蛋白質) 첨가군(群)에 있어서 더욱 높았으며 RBC, WBC, Hct과 Hb함량(含量)은 5식이군(食餌群) 모두 정상치(定常値)에 속(屬)하였다. Total serum protein과 albumin의 함량(含量)은 번데기단백질(蛋白質)의 첨가로 인해 현저히 증가(增加)하는 결과를 보여주고 있다. 이상의 결과로서 번데기단백질(蛋白質)은 대두단백질(大豆蛋白質)의 질(質)을 향상(向上)시킬 수 있는 단백질원(蛋白質源)으로 사료(思料)된다.

• 한국가금학회:학술대회논문집
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• 한국가금학회 2006년도 제23차 정기총회 및 학술발표회
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• pp.17-27
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• 2006

The effects of dietary betaine on performance, blood compositions, hepatic amino acid concentrations and hormonal secretions were examined in laying hens. Egg production was significantly higher in birds fed the 16.5 % protein diet compared to those fed 14.5 % protein diet(p<0.05), whereas dietary supplementation of betaine did not show any significant effect. The high level of protein and betaine supplementation significantly improved egg weight, egg mass and feed conversion(p<0.05), while eggshell breaking strength, eggshell thickness and Haugh unit were not influenced by betaine and dietary protein levels. Supplemental betaine did not affect serum total protein, albumin and BUN concentration. However, uric acid concentration significantly increased in 600 ppm betaine-fed groups(p<0.05). Concentrations of most hepatic amino acid were influenced by increased protein feeding and dietary betaine supplementation. Hormone studies recorded significantly higher serum and hepatocyte IGF-I concentration in 600 and 1,200 ppm betaine treatments(p<0.05) compared to those of control group. IGF-I mRNA gene expression of hepatocytes revealed statistically correlated increase in 600 and 1,200 ppm betaine-fed groups compared to the controls(p<0.05). Serum IGFBP-3 concentration was significantly elevated in 600 ppm betaine treatments. However, the secretion of IGFBP-1 in hepatocyte of laying hens fed with 600 and 1,200 ppm of betaine showed a significant decrease compared to the control group(p<0.05). Results of these study show that dietary betaine supplementation affects protein and hormone metabolism in laying hens.

• 대한의생명과학회지
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• 제12권3호
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• pp.197-200
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• 2006

We analyzed total protein (TP), albumin (AL), aspartic aminotransferase (AST), alanine aminotransferase (ALT), urea nitrogen (UN), creatinine (CRE) and serum protein electrophoretic fractions in sera of rural elderly patients to evaluate the health status in rural elderly patients. We observed that the frequencies of patients showed lower level of total protein, albumin, and both total protein and albumin than them of reference range were 20.3%, 22.8% and 19.0%, respectively. The rates of patients showed higher level of AST, ALT, both of AST and ALT, UN, creatinine and both of UN and creatinine than them of reference range were 33.8%, 40.0%, 30.0%, 17.7%, 15.2% and 7.9%, respectively. Comparison of protein fractions of each patient to reference range showed that 77.2% of patients showed normal in all of patterns. Few of patients showed abnormal pattern in albumin (13.6%), ${\alpha}1-globulin (0.0%),\;{\alpha}_2-g10bulin(1.1%),\;{\beta}-globulin(1.9%)\;and\;{\gamma}-globulin(6.2%)$. These data suggest that many of rural elderly patients may suffer from heart, liver and kidney diseases. The serum protein fractions are not typical criterion to evaluate the disease, but production of proteins in rural elderly patients may be affected by liver disease and kidney disease.

• Journal of Nutrition and Health
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• 제19권3호
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• pp.177-189
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• 1986

This study was undertalien to investigate the effects of dietary protein sources on protein, lipid, Ca. metabolism and immune response in college women (20), 16 of whom had Jupito constitutions and 4 had Hespero constitutions based on Kwon's theory . They were divided into 3 groups according to the main dietary protein source ; Beef group, Yellow tailrunner group and Soybean durd group. The menu of experimental diet of 3 group were same except their main dietary protein source(beef, yellow tailrunner, soybean curd). They were fed experimental diet for 4 days and their food intake was not restricted. Beef group in Jupito constitutions and yellow tailrunner group in Hespero constitutions were expected to present better effects than the other groups, because beef and yellow tailrunner are good for Jupito constitutions and Hespero constitutions, respectively. Results of Beef group in Jupito constitutions and Hespero constitutions, respectively. Results of Beef group in Jupito constitutions and yellow tailrunner group in Hespero constitutions were as following. 1) Calcium retention rate, Calcium apparent digestibility, serum Complement 3 concentration and LDL+ VLDLconcentration were higher in both groups. 2) Serum HDL concentration and immunoglobulin G concentration were lower in both groups than in the other groups. According to the main dietary protein sources, noted results were as following. 1) Serum total lipid and total cholesterol concentration were the significantly lowest in Soybean curd group. 2) Nitrogen retention rate was the significantly highest in Beef group.

• 한국농촌생활과학회지
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• 제6권1호
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• pp.31-39
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• 1995

This study was performed to observe how soy protein and calcium in rats fed unsaturated fat-enriched diet affect the total lipid and cholesterol contents of blood and tissues male Sprague-Dawley rats weighing approximately 220g were fed four purified diets which contained 18% (w/w) corn oil, 1% (w/w) cholesterol, two sources of protein : casein or isolated soy protein (ISP) and two levels of dietary calcium : 0.1% or 1.0%, first for four weeks (Expt. 1) and second for eight weeks (Expt. 2). The contents of total lipid, cholesterol and triglyceride in blood, liver, heart and feces were determined. After four weeks feeding, the serum lipid and cholesterol concentration was not significantly different among the groups. After eight weeks feeding, these concentrations were significantly lower in ISP-High calcium group. The serum lipid concentration was influenced by dietary protein sources and calcium levels. These results indicated that the hypolipidemic effects of soy protein and high calcium intake were partly due to decrease in lipid absorption and these effects were not detected at 4 weeks feeding but 8 weeks feeding.

• Nutrition Research and Practice
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• 제5권3호
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• pp.224-229
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• 2011

The prevalence of atopic dermatitis (AD) in school-age children has increased in industrialized countries. As diet is one of the main factors provoking AD, some studies have suggested that food additives in processed foods could function as pseudoallergens, which comprise the non-immunoglobulin E-mediated reaction. Eosinophil cationic protein (ECP) is an eosinophil granule protein released during allergic reactions to food allergens in patients with AD. Thus, serum ECP levels may be a useful indicator of ongoing inflammatory processes in patients with AD. The purpose of this study was to investigate the effect of consuming MSG in processed foods on serum ECP levels among children with AD. This study was performed with 13 patients with AD (age, 7-11 years) who had a normal range of total IgE levels (< 300 IU/ml). All participants ate normal diets during the first week. Then, six patients were allocated to a processed food-restricted group (PRDG) and seven patients were in a general diet group (GDG). During the second week, children in the PRDG and their parents were asked to avoid eating all processed foods. On the third week, children in the PRDG were allowed all foods, as were the children in the GDG throughout the 3-week period. The subjects were asked to complete a dietary record during the trial period. Children with AD who received the dietary restriction showed decreased consumption of MSG and decreased serum ECP levels and an improved SCORing score on the atopic dermatitis index (P < 0.05). No differences in serum ECP levels or MSG consumption were observed in the GDG. Serum total IgE levels were not changed in either group. In conclusion, a reduction in MSG intake by restricting processed food consumption may lead to a decrease in serum ECP levels in children with AD and improve AD symptoms.

• 한국동물학회지
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• 제18권1호
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• pp.9-18
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• 1975

成熟한 Sprague-Dawley 系인 雄性 흰쥐를 對照群과 實驗群으로 나누어, 對照群은 760 mmHg인 正常氣壓에 實驗群은 500 mmHg 및 380 mmHg의 低氣壓에 15時間(1日當 1時間)曝露시켰다. 曝露期間中 여러 時間區에서 總 血淸蛋白質量을 Kjeldahl 및 Biure 方法에 依해 Coleman Model 295 E Spectrophotometer로서 540 nm에서 測定하였으며, 血淸蛋白質의 電基泳動像은 Grassman-Hang (1960)方法에 依하여 barbital buffer(pH 8.6, 이온强度 0.05)로서 分離 測定하였다. 一般的으로 두 實驗群에 있어서 總 血淸蛋白質量은 對照群에 比하여 1日區에서 減少된 後, 2日區에서부터 增加 傾向이 나타나며, 5日區에서 부터는 對照群에 比하여 높은 恒定 持續性을 나타내었고, 한편 Albumin/Globulin 比에 있어서는 對照群에 比하여 1日區에서 增加를 나타내고, 그 後에는 徐徐히 減少하여 5日區에서 부터는 對照群에 比하여 낮은 恒定 持續性을 나타내었다. 特히 血淸蛋白質分劃에서 beta globulin 含量이 實驗期間中 兩群 共히 有意性있는 上昇을 보였다. 一般的으로 低氣壓인 380 mmHg群은 500 mmHg群에 比하여 顯著한 變化相을 가져왔으며, 上記한 低氣壓에 曝露 5日傾부터 馴化現象을 나타내는 것으로 생각된다.

• Maxillofacial Plastic and Reconstructive Surgery
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• 제41권
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• pp.46.1-46.9
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• 2019

Background: Oral squamous cell carcinoma (OSCC) constitutes a group of tumors that exhibit heterogeneous biology, histopathology, and clinical behaviors. Case presentation: A 73-year-old male had a whitish leukoplakia-like lesion around inflamed peri-implant area (#42, #43, and #44), and this lesion had transformed to OSCC within 3 years. He underwent mass resection, selective neck dissection, and reconstructive surgery. To detect any carcinogenesis progression, we examined the removed tumor tissue as well as the patient's preoperative and postoperative sera to identify causative oncogenic proteins using immunoprecipitation high-performance liquid chromatography (IP-HPLC). Conclusions: The protein expression levels of p53, E-cadherin, β-catenin, MMP-10, HER2, NRAS, Met, HER2, and ERb were significantly lower in the serum collected on postoperative day 10 than in the preoperative serum, and if these proteins are consistently not elevated in the serum 3 months after surgery compared with the preoperative serum, these proteins can be potential oncogenic proteins. However, we also found that the serum extracted 3 months after the operation had elevated levels of oncogenic proteins compared with that of the preoperative and 10-day postoperative serum indicating the possibility of tumor recurrence. At postoperative follow-up period, ipsilateral neck metastasis and second primary lesion were found and additional surgery was performed to the patient. IP-HPLC using the patient's serum shows the possibility of oncogenic protein detection. However, follow-up IP-HPLC data is needed to find out patient-specific prognostic factors.