Effect of Low Atmospheric Pressure on Serum Protein of Rats

低氣壓이 흰쥐의 血淸蛋白質에 미치는 影響

Male rats of Sprague-Dawley strain were subjected to low atmospheric pressures (500 and 300 mmHg)for an hour a day during 15 days. The serum protein patterns and the level of total serum proteins were determined at various time intervals during exposure. 1. When compared with control animals maintained at 760 mmHg, the low atmospheric pressure-exposed animal groups at 500 or 380 mmHg showed different changes in the serum protein patterns and the level of total serum proteins. 2. In the level of total serum preteins, a decrease resulted during the 1st day exposure of both groups, and slowly increased thereafter. From 5 to 15 days a higher steady state value than the control was obaserved. 3. In A/G ratio, the sera resulted in an increase in 1st day of both groups, and slowly decreased thereafter. From 5 to 15 days a lower steady state significantly than control value resulted. 4. Specifically, in the serum protein components, $\\beta$-globulin was increased during the experimental periods of both groups.

成熟한 Sprague-Dawley 系인 雄性 흰쥐를 對照群과 實驗群으로 나누어, 對照群은 760 mmHg인 正常氣壓에 實驗群은 500 mmHg 및 380 mmHg의 低氣壓에 15時間(1日當 1時間)曝露시켰다. 曝露期間中 여러 時間區에서 總 血淸蛋白質量을 Kjeldahl 및 Biure 方法에 依해 Coleman Model 295 E Spectrophotometer로서 540 nm에서 測定하였으며, 血淸蛋白質의 電基泳動像은 Grassman-Hang (1960)方法에 依하여 barbital buffer(pH 8.6, 이온强度 0.05)로서 分離 測定하였다. 一般的으로 두 實驗群에 있어서 總 血淸蛋白質量은 對照群에 比하여 1日區에서 減少된 後, 2日區에서부터 增加 傾向이 나타나며, 5日區에서 부터는 對照群에 比하여 높은 恒定 持續性을 나타내었고, 한편 Albumin/Globulin 比에 있어서는 對照群에 比하여 1日區에서 增加를 나타내고, 그 後에는 徐徐히 減少하여 5日區에서 부터는 對照群에 比하여 낮은 恒定 持續性을 나타내었다. 特히 血淸蛋白質分劃에서 beta globulin 含量이 實驗期間中 兩群 共히 有意性있는 上昇을 보였다. 一般的으로 低氣壓인 380 mmHg群은 500 mmHg群에 比하여 顯著한 變化相을 가져왔으며, 上記한 低氣壓에 曝露 5日傾부터 馴化現象을 나타내는 것으로 생각된다.