• Journal of Nutrition and Health
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• 제27권10호
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• pp.1048-1057
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• 1994

Nonobese NIDDM patients were studied were studied with respect to changes in visceral protein status, serum glucose and lipids and insulin secretion capacity before and after intake of enteral formula. Patients with renal or hepatic disease, gastrectomy, malabsorption, weight gain over past 6 months and poorly controlled blood glucose level were excluded. Eighteen patients served as case and administered, in addition of their usual diet, 400ml of enteral formula for 8 weeks. Another 18 patients participated in controls and had usual food intake for 8 weeks. In the begining, the levels of fasting and postprandial glucose, glycated hemoglobin, triglyceride, HDL, LDL, total cholesterol, albumin, total protein and transferrin and glucose response area on oral glucose tolerance test were not different between two groups. The response areas of insulin, C-peptide and free fatty acid and serum IGF-1 level were higher in the case than in the control group. Energy intake of patients given enteral formula exceeded their estimated energy requirements(108%) and they consumed a mean of 112g protein per day. Patients given enteral formula showed an increase in body weight(4.4%), serum transferrin(10%), IGF-1(13%) and triglyceride(34%) while controls showed no changes in those parameters at 8 weeks compared to initial values. There were no significant changes after 8 weeks in the levels of glucose, glycated hemoglobin, HDL, LDL, total cholesterol, total protein and albumin and response areas of glucose, insulin, C-peptide and free fatty acid in both groups compared to initial values. This study suggests that nutrition supplement with enteral formula can increase body weight and visceral protein status in nonobese NIDDM patients without changes in blood glucose. However, excessive calorie intake could temporarily increase serum triglyceride. In addition, this study indicates that serum transferrin and IGF-1 are more sensitive indicators to changes of protein intake than serum albumin and total protein.

• 생명과학회지
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• 제9권1호
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• pp.35-43
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• 1999

이탈리안 라이그라스로부터 갈변을 방지시켜 white LP를 제조하여 4주령의 Wistar 계 수컷 흰쥐에 14일 급여, 혈청지질농도 및 혈중 유리 아미노산 패턴에 대하여 단백질원으로 대두단백질 및 카제인 급여와 비교하였으며 혈중 유리 아미노산이 혈청 지질농도에 대하여 어떠한 관련이 있는지를 검토하였다. 갈변을 방지하여 조제한 LP 의 질소 함량 및 소화율이 거의 대두단백질 수준까지 높여 단백질의 품질에 대한 영향을 확인하였다. 혈청 총 콜레스테롤 및 LDL-콜레스테롤은 카제인군에 비하여 이탈리안 라이그라스 white LP 군이 유의적으로 낮고, 대두 단백질군과 같은 수준이었다. 이때 혈중유리아미노산을 비교한 결과, LP 군과 대두단백질군과는 거의 닮은 패턴을 나타내고 카제인군에 대해서는 glycine 만 높고 다른 아미노산은 차가 없든지 유의적으로 낮았다. 그리고, 카제인 수준을 15.0% 로 낮추어 대두 단백질과 비교하였다. 그 결과, 혈청 콜레스테롤 농도는 카제인군이 유의적으로 높고 혈중 glycine 농도가 낮고, methionine 농도의 높은 폐턴은 일치하였다. 이상의 결과로부터 혈중 유리아미노산 패턴은 급여 단백질의 종류에 의해 어느정도 규제되어지며 아미노산 패턴은 혈청지질과 어떠한 관련이 있는 것으로 인정되었다. 더욱이, LP 와 대두단백질의 혈중 유리 아미노산 패턴은 매우 유사한 것이 관찰되어 LP 의 혈청 콜레스테롤 강하작용의 메카니즘은 대두 단백질의 메카니즘과 유사한 것으로 생각되어진다.

• Nutrition Research and Practice
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• 제2권3호
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• pp.158-164
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• 2008

This study investigated that the antioxidative effect of freeze-dried cranberry powder against protein and lipid oxidation and ameliorative effect of serum lipid profile in rat fed atherogenic diet. Six weeks old male Sprague-Dawley rats were divided into the following four groups: normal diet group with 5% com oil(control), atherogenic diet group with 5% com oil, 10% lard, 1% cholesterol, and 0.5% sodium cholate(HFC), atherogenic plus 2% cranberry powder diet group(HFC+C2), and atherogenic plus 5% cranberry powder diet group(HFC+C5), and respective diet and water were fed daily for 6 weeks. After the experimental period, the serum lipid profile, such as total cholesterol, HDL-cholesterol, LDL-cholesterol, and triglyceride, ferric reducing ability of plasma(FRAP), plasma phenolics content, superoxide dismutase(SOD) activity, serum protein carbonyl and thiobarbituric acid reactive substances(TBARS) levels were examined. Total phenolic compound and total flavonoid levels in freeze-dried cranberry powder were 9.94 mg/g and 8.12 mg/g, respectively. Serum total cholesterol and LDL-cholesterol levels were not significantly different for cranberry powder treatment, but serum HDL-cholesterol level was significantly increased in HFC+C5 group compared with HFC group. Plasma FRAP value tended to be increased by cranberry powder treatment though there was no significant difference. Plasma total phenol concentrations and SOD activities were not significantly different among all groups. Serum protein carbonyl and TBARS levels were significantly decreased in HFC+C5 group compared with HFC group. Overall results suggested that freeze-dried cranberry powder might have the serum lipid improving effect, as well as anti oxidative effect demonstrated by its protective effect against protein and lipid oxidation.

• Journal of Nutrition and Health
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• 제14권3호
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• pp.136-145
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• 1981

This study was designed to observe the effects of both control and atherogenic diets on the cholesterol and triglyceride (TG) in serum and liver of adult rats fed diets supplying two levels of dietary fat and two different sources of dietary protein in early life. For the first experimental period, the rats were assigned into the four diet groups: High fat, casein (HC); High fat, gluten (HG); Low fat, casein (LC): Low fat, gluten (LG). Each group was subdivided into control and atherogenic groups for the second experimental period. Cholesterol and TG were determined in serum and liver after 7 hr fasting. The body weight gain was greater in the rats of the casein groups than those of the gluten groups tut not influenced by the level of the dietary fat. The difference in body weight from the quality of dietary protein in the first period was not disappeared even after the second period. After the first period, higher serum cholesterol was observed in the rats fed either casein or high fat diets. With the second experimental diet, rats fed atherogenic diet showed higher serum cholesterol concentration but lower serum TG levels compared to those fed control diet, regardless the diets fed in the first period. Serum cholesterol level of the rats of both groups which had been fed high fat diets in early life was increased compared to those of the low fat diet groups. This effect was more pronounced with the atherogenic diet groups than control groups. However, no differences were found in serum cholesterol levels resulted from the different types of dietary protein fed in the first period. Serum TG concentration was not influenced by the quality of protein and level of fat in the diet but seemed to be mere affected by the amount of carbohyrates in the diet. Liver cholesterol per unit weight was greater in the gluten diet groups than in the casein groups but total cholesterol was higher in casein fed rats. There were no differences in liver TG among the groups.

• 한국양식학회지
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• 제11권2호
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• pp.271-278
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• 1998

전복의 난황단백질의 특성을 조사하기 위하여 성숙한 참전복 암컷의 난소난 추출물로부터 sepharose Cl-4B gel chromatography을 사용하여 난황단백질을 분리하였다. 암컷과 수컷의 혈청과 난소난 추출물에 대한 항혈청을 이용하여 암수혈청과 난소난 추출물을 면역전기영동과 Ouchterlony 면역확산을 한 결과, 성숙한 암컷 혈청에는 암컷 특이 혈청 단백질(female specific serum protein) 이 존재하였으며, 이것은 난소난 추출물과 동일한 항원성을 가지고 있었다. 한 종류의 난황단백질이 난소난 추출물로부터 분리되었으며, 이 난황단백질은 SDS 전기영동에 의해 분자량이 각각 181kDa과 113kDa이 되는 2개의 subuit로 구성되어 있었다. 이들 난황단백질에 대한 항혈청은 성숙한 암컷 특이 혈청단백질과 간췌장 추출액과 침강반응을 보였으며 서로 교차반응이 일어났으나, 성숙한 수컷 간췌장 추출물에 대해서는 반응이 일어나지 않았다.

• Journal of Pharmaceutical Investigation
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• 제15권1호
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• pp.1-7
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• 1985

Previous studies show that the free (unbound) fraction of disopyramide in human serum is drug concentration dependent~ at corresponding serum disopyramide concentrations that are achieved clinically. $^1^{\sim}^3^)\;Moreover$, disopyramide free fraction values vary several fold at any given drug concentration in human serum and tend to decrease as serum cocentrations of alpha-I-acid glycoprotein(AAG) incrase.$^4^)$ A recent $study^5^)$ demonstrates that the free fraction of disopyramide inhuman serum increases almost 2-fold following the addition of $14.4{\mu}M/L$ mono-N-dealkyldisopyramide. These studies and others. $^6^),\;^7^)$ prompted the present investigation to characterize the protein binding of disopyramide in human serum and solutions of AAG in the presence of mono-N-dealkyldisopyramide (a major metabolite of, disopyramide) and to determine the utility of using commercially available alpha-I-acid glycoprotein for drug protein binding displacement studies. Because many basic and acidic compounds are known to bind to alpha-I-acid $glycoprotein^8^)$ the present study. was performed to determine whteher commercially available AAG would provide a convenient protein source for such binding studies.

• Journal of Nutrition and Health
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• 제34권7호
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• pp.741-747
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• 2001

The effects of fruiting body of Cordyceps militaris on the growth, the food intake, the food efficiency ratio, the lipid metabolism, the serum protein level ad enzyme activity in male rats were studied. Sprague-Dawley rats were fed four types of diets for five weeks, respectiely: a control diet, a control diet supplemented with 2%, 3% or 4% fruiting body of Cordyceps militaris(CF) powder. In rats fed 2% or 3% CF diets the body weight gain, the food intake, the concentratons of hepatic triglyceride and serum LDL-cholesterol, the atherogenic index, and the total lipid, total cholesterol, triglyceride and phospholipid in serum were similar to those in rats fed the control diet. Whereas, in 4% CF diet these were significantly decrased. But the all CF diets feeding could not decrease the food efficiency ratio, the weights of liver, pancreas, spleen, kidney and heart, and the concentration of serum HDL-cholesterol. Also, it was shown that the concentrations of glucose, total protein, albumin, urea and creatinine, and the GOT, GPT, LDH, ${\gamma}$-GTP and ALP activities were the same levels in serum of rats of fed all the experimental diets.

• 농업과학연구
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• 제37권2호
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• pp.267-270
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• 2010

This study was carried out to manufacture black charcoal board from domestic wood waste by using serum protein adhesive which is natural, environment-friendly and human-friendly. For the preparation of the serum protein adhesive, pig blood from slaughterhouse was centrifuged and serum was separated from corpuscles and concentrated to 30% by dry weight basis. The particle size of charcoal from domestic wood waste for this study was #6-60. Hot pressing schedule was $170^{\circ}C$ and 40kgf/$cm^2$ (1 min)-10kgf/$cm^2$ (2.5 min)-40kgf/$cm^2$ (5 min). The black charcoal board made by the addition of 13% serum protein adhesive on dry weight basis gave 41.76kgf/$cm^2$ of bending strength, 8.12kgf/$cm^2$ of internal bonding strength, and excellent gas adsorption and workability.

• 대한예방한의학회지
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• 제4권1호
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• pp.17-31
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• 2000

In order to investigate the effects of emitted-qi therapy(EQT) and cold water therapy(CWT) on the inhibition of the blood loss, the protein loss and the electrolyte loss in burned rats. The white blood cell, hematocrit and hemoglobin values in blood, and the total protein, albumin, globulin, $Na^+,\;Cl^-,\;K^+\;and\;Ca^{2+}$ values in serum were measured. The results were obtained as follows; 1. White blood cell count in blood was significantly increased in CWT group compared with control group. 2. Hematocrit value in bolld was significantly increase in EQT and CWT group compared with control group. 3. Hemoglobin value in blood was significantly increased in CWT group compared with control group. 4. Total protein value in serum was significantly increased in CWT group compared with control group. 5. Albumin value in serum was significantly increased in EQT and CWT group compared with control group. 6. Globulin value in serum tended to be increase in EQT and CWT group compared with control group, but it was not significant. 7. A/G ratio in serum was significantly increased in EQT and CWT group compared with contort group. 8. $Na^+$, value in serum was significantly increased in EQT and CWT group compared with ontrol group. 9. $K^+$, value in serum tended to be increased in EQT group compared with control group, but it was not significant. 10. $Cl^-$ value in serum was significantly increased in EQT and CWT group compared with control group. 11. $Ca^{2+}$ value in serum tended to be increased in EQT and CWT compared with control group, but it was not significant. According to the above results, the EQT and CWT inhibited the blood loss, the protein loss and the electrolyte loss. Therefore, it is considered that the EQT and CWT can be applied to the burn therapy.

• 대한한의학회지
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• 제18권2호
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• pp.283-315
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• 1997

The effects of Geumguesingitang and Dohongsamultang on rats with nephrosis induced by a single tail-intravenous injection of puromycin aminonucleoside(PAN), 2.5mg/l00g of body weight were evaluated in the present study. The effects of Geumguesingitang and Dohongsamultang on PAN nephrosis were evaluated by measuring (1)the concentrations of albumin, total protein, total lipid, cholesterol, triglyceride, creatinine, blood urea nitrogen(BUN) and uric acid in the serum, (2)the concentrations of protein, creatinine, glucose, occult blood and volume of the 24 hours urine and (3)the volume of intake water. The results are summerized as follows; 1. In the Control group as compared to the Normal. the amount of protein of 24 hours urine was significantly increased, the concentrations of albumin and total protein were significantly decreased. Total lipid, cholesterol and triglyceride in the serum were significantly increased. The concentrations of creatinine, BUN, uric acid in the serum, the amount of glucose and occult blood of 24 hours urine were also increased significantly. 2. In the Geumguesingitang group as compared to the Control, the increase in the amount of urinary protein during 24 hours induced by PAN was significantly suppressed, and the concentrations of total protein and albumin in the serum were significantly increased. The concentrations of total lipid, cholesterol and triglyceride in the serum were significantly inhibited. The decrease of the concentrations of creatinine and uric acid in the serum were also observed significantly. 3. In the Dohongsamultang group as compared to the Control, the increase of the amount of protein and glucose of the 24 hours urine induced by PAN were significantly inhibited, and the concentrations of total protein and albumin in the serum were increased significantly. The concentrations of total lipid, cholesterol and triglyceride in the serum were decreased significantly. The decrease of the concentrations of creatinine and uric acid in the serum were observed significantly. It can be inferred that Geumguesingitang has effects on improving proteinuria, hypoproteinemia effectively. It has an effect on hyperlipidemia significantly relieved. And relieving azotemia when nephrotic syndrome is accompanied by the acute renal failure. It can be inferred that Dohongsamultang improves hyperlipidemia effectively. It has effects on proteinuria, hypoproteinemia in nephrotic syndrome significantly relieved. And relieving azotemia when nephrotic syndrome is accompanied by the acute renal failure.