• 한국식품영양과학회지
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• 제23권6호
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• pp.894-898
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• 1994

To evaluate an effect of dietary protein on the liver damage, the bromobenzene was intraperitoneally injected to the rats fed a low or high protein diet and then the liver weight per body weight and serum levels of alanine aminotransferase (ALT) activities were determined to demonstrate the differences in liver damage between the groups fed low or high protein diet. Hepatic aniline hydroxylase (AH), glutthione (GSH) content and glutathione s-transferase(GST) activity were also determined to clarify causes of liver damage between the two groups. Increases of liver weight per body weight and serum ALT activities were higher in brombenzene treated rats fed low protein diet than those fed high protein diet. The increasing rate of hepatic AH activity was higher in bromobenzne-treated rats fed low protein diet than that in those fed high protein diet. Furthermore , hepatic glutathione contents and GST activities in bromobenzene-treated rats were higher in rats fed high protein diet than those fed low protein diet. In case of control group, the heaptic glutathione content and GST activity were also higher in rats fed high protein diet than those fed low protein diet.

• Journal of Microbiology and Biotechnology
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• 제26권6호
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• pp.1140-1147
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• 2016

The plasma and serum of Crocodylus siamensis have previously been reported to exhibit potent antimicrobial, antioxidant, and anti-inflammatory activities. During wound healing, these biological properties play a crucial role for supporting the formation of new tissue around the injured skin in the recovery process. Thus, this study aimed to evaluate the wound healing properties of C. siamensis plasma and serum. The collected data demonstrate that crocodile plasma and serum were able to activate in vitro proliferation and migration of HaCaT, a human keratinocyte cell line, which represents an essential phase in the wound healing process. With respect to investigating cell migration, a scratch wound experiment was performed which revealed the ability of plasma and serum to decrease the gap of wounds in a dose-dependent manner. Consistent with the in vitro results, remarkably enhanced wound repair was also observed in a mouse excisional skin wound model after treatment with plasma or serum. The effects of C. siamensis plasma and serum on wound healing were further elucidated by treating wound infections by Staphylococcus aureus ATCC 25923 on mice skin coupled with a histological method. The results indicate that crocodile plasma and serum promote the prevention of wound infection and boost the re-epithelialization necessary for the formation of new skin. Therefore, this work represents the first study to demonstrate the efficiency of C. siamensis plasma and serum with respect to their wound healing properties and strongly supports the utilization of C. siamensis plasma and serum as therapeutic products for injured skin treatment.

• 한국지역사회생활과학회지
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• 제15권3호
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• pp.113-120
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• 2004

This study is to investigate the effect of dietary L-carnitine supplementation on lipid metabolism in rats fed with isolated soy protein and casein for their source of protein. Four experimental groups were organized and each group had eight Sprague-Dawley male rats with the initial weight of around 180g. The four groups were CO (casein only supplemented group); CC (casein and 3% L-carnitine supplemented group); ISO (isolated soy protein only supplemented group); ISC (isolated soy protein and 3% L-carnitine supplemented group). All groups were supplemented with the experimental diet for four weeks and carnitine comprised 3% of. their diet. The results were as follows; 1. There was no significant difference in food intake among the groups. 2. Final weight gain was significantly lower in the groups supplemented with isolated soy protein than in the groups supplemented with casein (P<0.05). The groups with supplemented casein and carnitine showed the effect of weight reduction (p<0.05). 3. Food efficiency ratio was lower in the groups supplemented with isolated soy protein than in the groups supplemented with casein (p<0.01). The groups supplemented with casein and carnitine showed low food efficiency ratio. 4. The serum total lipid was higher in the groups supplemented with casein than in the groups supplemented with isolated soy protein (p<0.05). 5. Serum total cholesterol was higher in the groups supplemented with casein than in the groups supplemented with isolated soy protein. 6. There was no significant difference in triglyceride, HDL-cholesterol, and LDL-cholesterol in serum among the groups. 7. Out of the groups supplemented with isolated soy protein the total cholesterol level in liver was low in the groups to which carnitine was supplemented (p< 0.05). However, there was no significant difference of liver total lipid and triglyceride among the groups. 8. There was no difference in TBARS levels and GSH-Px activities in liver among the groups.

• Journal of Nutrition and Health
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• 제31권3호
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• pp.243-252
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• 1998

This study was conducted to investigated the short-term effects of early weaning and protein intake on organ and cell growth, nitrogen metabolism and physiological functions of rats. Five groups of early weaned rats separated from the dam on the 15th day postpartum were each given one five diets consisting of either one of the three levels of casein-low(8%), -normal (16%), and -high(32%), or a normal level (16%) of isolated soy protein(ISP) or egg yolk protein, for 7 days. The normal weaned rats were fed maternal breast milk for three weeks from birth. On the 22nd day postpartum , all the rats were sacrificed . The weight gain of the early weaned rats, especially the ones fed high protein, was observed to be significantly lower than that of the normal weaned rats. By the 15th day, of early weaning and especially in the ISP-fed rats, the total DNA contents of liver and kidney, which may be said to represent an index of cell numbers, significantly decreased, but their fresh and dry weight and protein/DNA ratio, allegedly representing an index of cell size, significantly increased , not affecting the cell number and cell size of brain. There were no differences in total serum protein and albumin concentrations between early and normal weaned rats. In the early weaned rats observed , the serum urea N and $\alpha$-amino N concentrations significantly increased in high protein-fed rats, and decreased in low protein-fed rats. Another observation was that no significant difference was noticed as regards to serum GOT activity, total bilirubin, uric acid, and creatinine concentration, which may represent indices of liver and kidney functions, among rat groups, GPT activity was an exception . These results suggest that premature weaning and the quality and quantity of dietary protein significantly affect organ and cell growth and nitrogen metabolism but does not seriously affect physiological functions in the neonatal development of rats.

• 한국수산과학회지
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• 제25권5호
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• pp.432-442
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• 1992

동남참게, Eriocheir japonicus의 난황단백전구체 합성부위를 면역조직화학적인 방법으로 구명하기 위하여, 난황단백질을 정제하고, 이 단백질에 대한 항혈청을 제작하여 난황단백질에 대한 면역학적인 성질을 밝힌 후, 난황단백전구체의 항체가 존재하는 항 난황단백혈청을 사용하여 PAP(peroxidase-antiperoxidase)법으로 생식소, 간췌장, 근육조직을 대상으로 면역조직화학 반응을 시켰다. 성숙한 암, 수혈청에 대한 면역전기영동과 Ouch-terlony 면역확산 실험에 의하여 암혈청내에는 암 특이성혈청단백질이 있음이 밝혀졌다. 암, 수혈청에 대한 항혈청과 난황형성중인 암혈청에 대한 조난황단백추출액에 대하여 면역전기영동과 Ouchte-rlony 면역확산 실험을 행한 결과, 암 특이성혈청 단백질은 난황단백전구체임이 밝혀졌다. 조난황단백추출액으로 부터 DEAE 이온교환 chro-matography와 sepharose CL-4B gel 여과법에 의하여 난황단백질을 정제하였으며, 이 난황단백질의 분자량을 sepharose CL-4B gel 여과법에 의하여 추정한 결과 분자량은 약 245,000 dalton이었다. 또한 난황단백질은 면역학적인 분석에 의하여 난황 단백질에 대한 항혈청에는 난황단백전구체에 대한 항체가 존재하고 있음이 증명되었다. 난황단백질에 대한 토끼 항혈청을 이용하여 PAP법에 의한 면역조직화학반응을 시킨 결과, 난황형성중인 난모세포와 성숙한 암참게의 간췌장은 양성반응이 나타는데 반하여 난소내의 여포세포층과 난황형성전 난모세포, 근육층 그리고 성숙한 수참게의 간췌장은 전혀 양성반응이 나타나지 않았다. 따라서 본 종의 난황단백전구체의 합성부위는 간췌장임을 알 수 있다.

• Journal of Nutrition and Health
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• 제27권1호
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• pp.90-99
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• 1994

This study was carried out to examine how dietary protein and calcium levels in rats fed fat-enriched diet affect the total lipid and cholesterol contents of blood and tissues. Male Sprauge-Dawley rats weighing approximately 200g were fed six purified diets which contained 18%(w/w) beef tallow, 1% (w/w) cholesterol, two source of protein, casein or isolated soy protein (ISP) and three levels of dietary calcium, 0.1%, 0.4% and 1.0%, first, for four weeks, and second, for eight weeks. The contents of the total lipid, cholesterol and triglyceride in blood, liver, heart and feces were determined. After four weeks feeding serum lipid and cholesterol concentrations significantly decreased in rats fed 1.0% (w/w) level calcium, regardless of dietrary protein sources. After eight weeks, these concentrations were significantly lower in the rats fed soy protein than in casein-fed rats. As dietary calcium level increased serum and tissue lipid and cholesterol contents were decreased and fecal lipid excretion increased. It is concluded that hypolipidemic and/or hypocholesterolemic effects of soy protein and calcium were partly due to decrease in lipid absorption.

• KSBB Journal
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• 제13권2호
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• pp.125-132
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• 1998

Protein affinity membrane was prepared via the coating of chitosan gel on the porous flat polysulfone membrane surface, followed by the immobilization f the reactive dye (Cibacron Blue 3GA) to the chitonsan gel. The maximum protein binding capacity of affinity membrane was about 70${\mu}g/cm^2$ determined by the batch adsorption experiments of human serum albumin (HSA). Using module of this membrane, the characteristics of protein chromatography were investigated through the experiments of elution and frontal chromatography of HSA. This membrane module promises as a chromatography column, since it represented a lower pressure drop and a greater reproducibility. The protein separation ratio was significantly influenced by the flow rate of mobile phase and the injection quantity of HSA. The dynamic protein binding capacity of module decreased from the equilibrium binding capacity with increasing flow rate and approached the value of 15 - 20 ${\mu}g/cm^2$ for flow rates above 6 mL/min.

• The Korean Journal of Ecology
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• 제10권4호
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• pp.205-213
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• 1987

In order to investigate the effects of red ginseng extract to methyl mercury toxicities in mice, the serum lipoproteins, tissure protein patternsm and growth rates were studied. Animals wee divided into 3 groups of the control, group I treated with methyl mercury chloride only, and group II treated together with methyl mercury chloride and red ginseng extract. In serum lipoprotein fractions of group I, beta lipoprotein fraction was increased and pre-beta lipoprotein fraction was decreased in comparision to those of the control. However, there was almost no difference in quantities of serum lipoprotein fractions between the control and group II. Total pretein contents of groups I and II were increased in liver and those of groups I and II in the kidney were decreased. However, in comparison to group I, total protein contents of group II in the liver and kidney were similar values with those of the control. Percentage of tissue protein fractions between control and group I in the liver and kindey showed considerable difference. On the other hand, the percentage of protein fractions of group II approximated to that of the control. Daily average growth rate of body weight in group II was similar to the control, but that of group I was decreased significantly in comparison to the other 2 groups.

• 한국식품영양과학회지
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• 제27권3호
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• pp.525-530
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• 1998

The effects of godulbaegi(Ixeris sonchifolia H.) powder on body weight gain, lipid and protein concentrations, and enzyme activities were studied in growing male rats for 4 and 8 week. The fats (Sprague-Dawley) were fed diets containing 5% godulbaegi or without. The body weght gain, food efficiency ratio, protein efficiency ratio, hemoglobin concentration and hematocrit value of rats fed godulbaegi diet were similar to the control diet. The ratio of spleen weight to body weight in godulbaegi group for 8 weeks was greater than in that of control group, but they were similar both feeding groups in liver and kidney. The concentrations of the total lipid and triglyceride in serum were increased in rats fed godulbaegi diet for 4 week than in those fed control diet, but those were decreased in rats fed godulbaegi diet for 8 week than in those fed the control diet. The concentrations of the phospholipid, total cholestrol and HDL-cholestrol in serum were decreased in rats fed godulbaegi diet for 8 week than in those fed control diet. The HDL-cholestrol/total cholestrol ratio and athergenic index were similar to both diet groups. There were no differences in growth, serum total protein, albumin concentrations, aspartate aminotransferase and alanine aminotransferase activities between rats fed godulbaegi diet and those fed the control diet.

• 대한가정학회지
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• 제30권4호
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• pp.89-105
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• 1992

The purpose of this study was to see the effect of oral administration of reserpine (2mg/d) and tryptophan (40.35mg/d) on the serum amino acid concentrations and organ composition, food consumption, body weight, blood hematocrit(Hct) and hemoglobin(Hb) levels in Sprague-Dawley rats fed 6% or 20% casein diet. Any adverse effects of reserpine and tryptophan were not observed in animals, except that liver fat contents were increased in low protein group. In other words the administration of typtophan decreased liver fat contents in 6% casein and reserpine-treated 20% casein groups, but increased in reserpine-treated 6% casein group. But the low protein diet had significant adverse effects in animals. The 6% casein diet, therefore, had a tendency to decrease food consumption and body weight. The simillar tendency was shown in serum essential amino acid concentrations, organ weight and protein contents of liver and muscle. From the results, it would be safe to conclude that the oral administration of large deses of reserpine and tryptophan did not induce such a signifcant malnutrition as the low protein diet did.