• Journal of Nutrition and Health
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• 제12권4호
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• pp.29-42
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• 1979

In order to observe metabolic effects of an oral conceptive, Norinyle, on female Guinea pig, the changes of ascorbic acid amount and alkaline phosphatase activity in the liver and serum were determined, and histochemical changes of the uterus were observed by microscopic and electronmicroscopic methods by administration of Norinyle with or without ascorbic acid. The results obtained are summerized as follows: 1) The metabolic changes were clearly influenced by the administration of Norinyle alone, but the changes were diminshed by administration of Norinyle with ascorbic acid. 2) The adimnistration of Norinyle influenced to increases the requirment of ascorbic acid in the liver. 3) The uterus weight of the Norinyle administered group was much increased, while the weight was less increased in the group of administered Norinyle with ascorbic acid than the control. 4) The Norinyle administration was brought about an atrophy of endometrium, of uterus especially, functional layer, that the formation of glands were inadequately and the fromation of basal layer and stroma were diminished. 5) An acute infarction on the all layers of the uterus was developed at 9th and 25th days of Norinyle administration and 20th day of Norinyle with ascorbic acid administration. 6) A hypertrophy of stromal and endovascular cells were observed on the groups administered of Norinyle alone(group II ) or Norinyle with ascorbic acid(group IV). 7) It was observed that amount of collagen fiber in the basal and muscular layeres of uterus were diminished under a microscopical observation by the special stained specimen on the Norinyle administered group, but the amount and distribution of reticulin fiber were not changed significantly. 8) The fille structure of outer functional layer of the uterus were significantly changed by administration of Norinyle which were shown irregurarity of nuclear membrane, poor development ana significant expansion of enaoplasmic reticulum, decreases of the amount of ribosome due to slip off, increases of the number of dense bodies, obvious formation of vaccule, an4 decreases the amount of collagen in inner and outer layer of the stroma. 9) The amount of ascorbic acid in the serum did not much changed but the amount in the liver was much decreased by the administration of Norinyle, And the administration of Norinyle with ascorbic acid induced for a significant diminishing on the changes of uterus which might be able to developed by the administration of Norinyle alone.

• 생명과학회지
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• 제15권1호
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• pp.1-8
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• 2005

이황화메틸의 반복 흡입노출에 의한 아급성 독성 잠재력을 평가하기 위해 암수 랫드에게 0, 5, 25 및 125 ppm용량으로 21일간 반복 흡입노출하고, 일반증상과 체중, 사료섭취 량, 혈액치, 혈청생화학치 및 부검소견을 관찰하였다. 시험결과, 랫드에게 이황화메틸을 3주간 반복 흡입노출하면 125 ppm의 농도에서 체중증가의 억제와 사료섭취 량의 감소를 유발하나, 혈액 및 혈청생화학치에는 어떠한 이상도 유발하지 않는 것으로 나타났다. 본 시험 조건 하에서 이황화메틸의 표적 장기는 관찰되지 않았으며, 무해용량은 암수 모두 25 ppm으로 사료된다.

• Journal of Nutrition and Health
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• 제13권2호
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• pp.82-91
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• 1980

The aim of this study was to define the range of the menstrual blood loss(MBL), and the upper limit of the MBL associated with iron deficiency anemia in Korean women, For this purpose, healthy unmarried women between the age of 15 to 26 years were selected from July to September, 1979 and results obtained were used for determining baseline MBL and its relationship to iron nutriture. One houndred forteen women were tested for their MBL and hematological and biochemical data such as hemoglobin(Hb) concentration, hematocrit, mean corpuscular hemoglobin concentration(MCHC), and serum iron and ferritin concentration. This study was partly supported by World Health Organization. The results of this study are summarized as follows: 1) The mean values and standard error of mean of MBL were $30.7{\pm}2.19ml$ for total subjects, $30.4{\pm}3.14ml$ for sutjects below 20 years of age and $30.3{\pm}2.49ml$ for subjects aged more than 20 years. No significant variation was noted for amount of MBL between two age groups. 2) The median value and 95th percentile value of MBL were 26.5ml and 69.0ml respectively for total subjects. Maximum number (24.6%) of the subjects fell in group with 10-20ml of MBL while 10.5% showed MBL above 50m1. 3) Mean value of the duration of menstruation for total subjects was 4.34 days. 4) It was observed that mean values of Hb concentration, hematocrit and MCHC were decreased slightly in subjects showing MBL more than 40m1. Serum iron and ferritin levels were markedly decreased in women with MBL above 40m1 and 20-30ml, respectively. 5) In subject population showing MBL above 50ml, the frequency of subjects with hematological and biochemical data below anemia criteria were remarkably increased. 6) Although the number of subjects tested was small and the subject selection was not done by random stratified sampling from a population group, this study suggested that the upper normal limit of MBL of Korean women seems to be in the range of 40-50ml.

• 한국산학기술학회논문지
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• 제17권2호
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• pp.386-393
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• 2016

최근, 복부초음파로 인해 담낭용종의 발견률이 높아지고 있다. 담낭용종의 위험인자에 대한 다양한 연구가 보고 되었지만 생화학검사, 생활습관을 포함한 대사증후군과 담낭용종의 관계에 대한 연구가 미흡한 실정이다. 본 연구의 목적은 생활습관, 생화학적 혈액검사를 포함한 대사증후군과 담낭용종과의 관련성을 알아보는데 있다. 2013년 1월부터 2013년 12월까지 건강검진 수진자 중 복부초음파검사를 시행 받아 담낭용종의 진단을 받은 596명을 대상으로 나이, 성별, 흡연, 음주, 운동의 일반적인 특성과 신장, 체중, 혈압, 허리둘레, 체질량 지수 등의 신체 계측을 하였으며, 동시에 채혈을 통한 공복 시 혈당과 여러 가지 혈액검사 및 대사증후군 여부를 분석하여 담낭용종과의 관계를 알아보았다. 그 결과 남성, 흡연자, 비음주자에서 유의하게 높았으며, 체질량지수가 높을수록, 대사증후군이 있는 집단이, HBsAg 양성군에서 높았고, 위험도는 남성이 여성보다 1.8배, 대사증후군이 있는 군이 없는 군보다 2.3배, HBsAg이 양성인 군에서 2.6배 높게 나타났다.

• 동의신경정신과학회지
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• 제23권2호
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• pp.99-120
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• 2012

Objectives : The oriental medicine Jangwonhwan, a boiled extract of 12 medicinal herbs/mushrooms, has been prescribed to patients with cognitive dysfunction, as originally described in the Korean medical text, DonguiBogam(amnesia chapter). Recently, a modified formula of Jangwonhwan (LMK02-Jangwonhwan) consisting of seven medicinal plants/mushrooms, was shown to reduce the ${\beta}$-amyloid deposition in the brain of Tg-APPswe/PS1dE9 mouse model for Alzheimer's disease. The toxicity of LMK02-Jangwonhwan was investigated in SD rats, by a daily oral administration for 13 weeks and NOAEL(No observed adverse effect dose), a definite toxic dose and target organ, as well. Methods : Quality control of the tablet form of LMK02-Jangwonhwan was established by estimating the indicative components, Ginsenoside Rg3 of Red Ginseng and Decursin of Angelicagigas Nakai. The toxicity of LMK02-Jangwonhwan was investigated in 6 week old, specific pathogen free (SPF), Sprageu-Dawley rats by oral administration. Each test group consisted of 10 male and 10 female rats. The groups received doses of 500, 1,000 or 2,000 mg/kg/day of test substance for 13 weeks. The clinical signs, death rate, body weight, food consumption, ophthalmic examination, urinalysis, hematological and serum biochemistry, organ weight and pathological changes were examined and compared with those of the control group. Results : The 13-week repeated oral treatment doses didn't result in any specific symptoms or death. There were no significant changes in the rat's weight and food consumption. Further, ophthalmic examination, urinalysis, hematological, serum biochemistry test and organ weight revealed no significant differences. Conclusions : The no-observed-adverse-effect level(NOAEL) of LMK02 for male and female Sprague-Dawley rats was determined as 2,000mg/kg/day and the target organ wasn't confirmed. Because no significant adverse effects were observed, the target organ could not be determined.

• 동의생리병리학회지
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• 제24권6호
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• pp.1034-1041
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• 2010

The oriental medicine Jangwonhwan, which is a boiled extract of 12 medicinal herbs/mushroom, has been prescribed for patients with cognitive dysfunction and it is originally from the Korean medical text, DonguiBogam(amnesia chapter). Recently, a modified recipe of Jangwonhwan (LMK02-Jangwonhwan) consisting of seven medicinal plants/mushroom, was shown to reduce ${\beta}$-amyloid deposition in the brain of Tg-APPswe/PS1dE9 mouse model of Alzheimer disease. The toxicity of LMK02 was investigated in SD rats by oral repeated adminstration for 4 weeks and we tried to determine test does for 13 weeks repeated study. Quality control of tablet form of LMK02 was established by estimating indicative components, Ginsenoside Rg3 of Red Ginseng and Decursin of Angelicagigas Nakai. The toxicity of LMK02 was investigated in 6 weeks old specific pathogen free (SPF) Sprageu-Dawley rats by oral administration. Each test group were consist of 5 male and 5 female and they received doses of 500, 1,000 and 2,000 mg/kg/day of test substance for 4 weeks. The clinical signs, death rate, body weight, food consumption, ophthalmic examination, urinalysis, hematological and serum biochemistry, organ weight and pathological changes were examined and compared with those of control group. Urinalysis : We observed increase of PRO(p<0.01), SG(p<0.01) in female rats of 1,000 mg/kg/day and 2,000 mg/kg/day(p<0.01). Also, we observed increase of pH and KET in female rats of 1,000 mg/kg/day(p<0.05) and of 2,000 mg/kg/day(p<0.01). WBC in female rats in 1,000 mg/kg/day and 2,000 mg/kg/day were on increase. Hematological test : We observed increase of MCV in male rats of 250 mg/kg/day. (p<0.05) Serum biochemistry test : We found increase of CHO in female rats of 2,000 mg/kg/day(p<0.05). During the experimental period, there were no animals dead or moribund. There were no treatment related changes of general symptom, food and water consumption, organ weight and autopsy According to the results of 4-week repeated dose range finding study, the highest dose was established as 1000 mg/kg for 13-week repeated dose toxicity study and we determined to put 2 more groups by common ratio two.

• 한국수정란이식학회지
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• 제21권3호
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• pp.225-231
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• 2006

본 연구는 제주도의 흑우에서 다배란을 유기한 후 발정기 동안 혈중 호르몬의 농도 검사, 혈액 생화학 검사, 체내 수정란 회수율 등을 관찰하였다. FSH를 4일간 8회 50 mg씩 주사하여 다배란을 유기하였다. 성호르몬의 측정은 radioimmunoassay (RIA)법으로 측정하였으며 혈액 생화학치는 자동혈청 분석기로 측정하였다. 인공 수정 후 7일에 수정란을 비외과적 방법으로 회수하였다. 본 연구의 결과는 다음과 같았다. 1. 혈중 호르몬의 농도를 측정한 결과 progesterone농도는 day -11에 $7.2{\pm}3.8ng/ml$ 이었으며 day -2에 급격히 감소하여 day 0에는 $0.2{\pm}0.1ng/ml$로 최저치를 유지하다가 day 2 후 상승하였다. Estradiol의 농도는 day -11에 $10.6{\pm}4.3pg/ml$였으며 day 0에는 $15.0{\pm}2.1pg/ml$ 로 최고치를 기록했다. 2. 다배란처리 후 발정 주기 동안의 AST, ALT, BUN, creatine, cholesterol, albumin 및 total protein등 혈액 생화학치의 변화를 측정한 결과 각 혈액 생화학치의 변화는 정상 소의 정상 범위 내 있었으며 유의적 차이는 없었다. 3. 공란우 12두에서 총 62개의 수정란을 회수하였다. 이중 이식 가능한 수정란은 37개(59.7%)였다. 본 연구의 결과로 제주흑우에서 다배란을 유도한 후 발정기 중 호르몬과 혈액생화학치의 변화양상을 알 수 있었다.

• 한국임상수의학회지
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• 제23권1호
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• pp.61-64
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• 2006

체중 4.65kg, 나이 13 개월의 수컷 페키니즈 견이 무뇨 증상으로 건국대학교 수의과대학 부속 동물병원에 내원하였다. 신체 검사상에서 복부 촉진을 통해 심각한 방광 팽창을 확인 하였다. 방광 내 요도 카테터 삽입을 시도 하였으나 실패 하였다. 혈액 화학 검사에서 BUN농도(35.6mg/dl)와 크레아틴농도(1.9mg/dl)가 각각 상승된 것으로 나타났다.단순 방사선 및 요도조영술에서 심한 방광팽창 및 막성 요도의 시작 부분이 폐쇄된 소견을 보였다. 수술동안 투시기를 이용 방광요도조영술과 요도조영술을 동시에 실시하여 본 바 폐쇄 부분 길이가 13mm임을 확인하였다. 골반강내 위치한 요도 폐쇄 부위가 피부절개선상까지 견인으로 노출이 가능하여 치골절골술 없이도 요도절제 및 요도문합을 실시할 수 있었다. 요도를 5-0 polyglycolic acid로 문합하고 이 부위를 대망막으로 감쌌다. 수술 후 첫째 날, 식욕이 회복 되었고, 5일 째 비정상 범위를 보였던 혈청 화학 수치들이 정상을 보였다. 그러나 요도 카테터를 제거한 후 약간의 요실금이 관찰 되었다. 7일 째 뇨 분석 결과가 정상 수치를 나타냈고, 14일 째 배설성 요로 조영술을 통해 수술 부위에 소변 누수가 없음을 확인 하였다. 21일 째, 더 이상의 요실금이 확인 되지 않았으며, 1년이 지난 현재 재발없이 정상적인 배뇨와 건강한 상태임을 확인할 수 있었다.

• 생명과학회지
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• 제14권4호
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• pp.547-552
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• 2004

본 연구는 살충제 amitraz의 피하투여 급성독성을 평가하기 위해 0, 2, 10 및 50 mg/kg용량으로 1회 피하투여한 후, 사망률과 일반증상, 체중, 혈액 및 혈청생화학 및 부검소견에 미치는 영향을 평가하였다. 시험결과, 비글 개에 있어서 amitraz의 단회 피하투여는 식욕부진과 침울, 구토, 유루, 운동성감소, 보행실조, 투여부위의 부종, 종괴 및 농양형성, 사지의 부전마비, 횡와위, 빈사, 사망, 체중감소, 투여부위의 근육출혈과 염증, 그리고 간과 신장의 충혈을 유발하는 것으로 나타났다. 반면, 투여 후 14일째에 생존동물의 혈액 및 혈청생화학치에서는 시험물질의 투여와 관련된 어떠한 변화도 인정되지 않았다. 결론적으로 본 시험조건하에서 비글 개에 대한 amitraz의 반수치사량은 22.3 mg/kg (95% 신뢰 한계: 산출되지 않음)이고, 무해용량(no-observed-adverse-effect level)은 2 mg/kg 이하로 사료된다.

• Journal of Animal Science and Technology
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• 제66권1호
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• pp.79-92
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• 2024

Global warming is a key challenge subjecting animals to heat stress conditions resulting in multiple physiological alterations in tropical climate. Dietary approach seems to be the more friendly approach to curb the adverse effects of heat stress in rabbits. Some herbs have been categorized to have high potential for promotion of immune responses for amelioration of heat stress. Thus, this research aims to evaluate the potential of Mistletoe (Viscum album), Moringa (Moringa oleifera) and Phyllanthus (Phyllanthus amarus) leaf meal as herbal supplements for the alleviation of heat stress in female rabbits by measuring improvement in sex and stress hormonal responses in serum biochemistry. 80 Rabbit does were exposed to 4 dietary groups supplemented with each of Mistletoe, Moringa, Phyllanthus and a control in an 84-day trial at the summit of thermal stress in South west Nigeria. Growth indices were monitored throughout the study, blood samples were compiled at the end of the trial to assess serum biochemistry, stress and sex hormonal responses of the Does using standard protocols. The results revealed that final weight and weight gain of Does fed on Phyllanthus were significantly (p < 0.05) higher (11.46% and 14.25%, respectively) than Does on control. The herbal supplements enhance glucose, protein, albumin and globulin, reduced cholesterol, and creatinine of Does under heat stress conditions. Among the herbal treatment groups, mistletoe, moringa and phyllanthus had 12.42%, 18.39% and 16.90%, respectively, lower corticosterone than control groups which had 39.76ng/ml. Triiodothyronine of Does fed control were significantly (p < 0.05) lower than Does on Moringa oleifera and Phyllanthus amarus supplements. Estradiol and Follicle stimulating hormone of rabbit Does fed on moringa supplement were significantly (p < 0.05) higher than other treatments. In conclusion, the herbal supplements tend to mitigate the detrimental outcome of thermal stress on Does by suppressing stress hormones. Moringa oleifera and Phyllanthus amarus enhanced sex hormones while Phyllanthus amarus confered growth promoting effects on the Does.