• Applied Microscopy
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• v.37 no.2
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• pp.103-109
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• 2007

The ultrastructure of submandibular gland was examined in the Korean spider shrew, Sorex caecutiens. The submandibular gland wat composed of acini and salivary ducts. A submadibular acinus was a mixed gland having serous demilune cells and mucous cells that were filled with well developed rER, mitochondria and large amount of dense secretory granules. Serous acinar granules were oval shape without distinct limiting membrane on the border and it had only coarse specks with various density. Mucous acinar granules were oval shape without distinct limiting membrane and had a variety pattern with several thin or transparent bands into the homogeneous dense matrix. Thus submandibular acinar granules of S. caecutiens belonging to subfamily Soricinae were distinct from the other mammalian species including Crocidurinae, because of the absence of limiting membrane of acinar granules and specific pattern of mucous acinar granules. Granular duct cells had large amount of small granular vesicles and several characteristic structures of granule which were revered with stratified limiting membranes and filled with coarse serous-like granule or homogeneous matrix.

• Applied Microscopy
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• v.35 no.4
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• pp.91-97
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• 2005

The acinar cells and secretory granules of the parotid salivary gland were examined in the lesser white toothed shrew, Crocidura suaveolens and the big white-toothed shrew, C. lasiura. The parotid gland of both species were a serous gland having only one kind of serous acinar cells, and had conventional arrangement of acini and intercalated, granular and striated ducts. In case of C. suaveolens, serous acinar cells had well developed rER, prominent Golgi complex, several large mitochondria and abundant moderate dense secretory granules with various stages of the maturing or fusing process. Immature acinar secretory granules were only or mainly filled with fine strong dense specks and had an indistinct limiting membrane, and mature granules were filled with homogeneous pale large round center and had fine strong dense specks at the periphery of the homogeneous pale center and a distinct limiting membrane. In case of C. lasiula, serous acinar cells had well developed rER, prominent Golgi complex, several large mitochondria and abundant dense secretory granules with maturing or fusing process. Immature acinar secretory granules were only filled with pale rough specks and had an indistinct limiting membrane, and mature granules were only filled with rough dense specks and had a distinct limiting membrane. Eventually The acinar secretory granules of C. suaveolens were seen moderate at the light and ultrastuctural level, those of C. lasiura were strong dense at the light microscopic level and dense at the ultrastructural level.

• The Korean Journal of Cytopathology
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• v.9 no.1
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• pp.1-14
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• 1998

The cytologic distinction of carcinoma cells from reactive mesothelial cells can be difficult, especially in specimens containing abundant reactive mesotheilal cells and inflammatory cells with scant carcinoma cells. This study evaluates the usefulness of mucin and immunocytochemistry for discrimination between reactive mesotheilal cells and carcinoma cells, and sensitivity and specificity of these stains for the detection of metastatic carcinoma in serous effusions. Immunocytochemical panel including mucin cytochemistry with the periodic acid-Schiff(PAS) reaction after or without diastase digestion was undertaken on 127 serous effusion specimens with histologically confirmed diagnoses. The specimens including cell smears and cell blocks were stained with PAS and antibodies to carcinoembryonic antigen(CEA), epithelial membrane antigen(EMA), cytokeratln(CK), and vimentin. The sensitivities of these stains for metastatic carcinoma(127 cases) were 49%(46/94) in PAS, 48%(60/124) in CEA, 89%(97/109) in EMA, 88%(93/106) in CK, and 25%(20/81) in vimentin. The sensitivities of stains for reactive mesothelial cells(36 cases) were 19%(7/36) in EMA, 78%(28/36) in CK, and 75%(27/36) in vimentin. The PAS and CEA stains were not reacted with all cases of benign reactive serous effusions containing abundant reactive mesothelial cells. The specificities of stains for metastatic carcinoma(127 cases) were 100% in PAS, 100% in CEA, 81% in EMA, 22% in CK, and 25% in vimentin. The optimal combination of stains for use in a panel was PAS and CEA. Combined results from these two stains yielded an advanced sensitivity of 8% in PAS and 4% in CEA for metastatic carcinoma. EMA was also cosiderably useful for identification of carcinoma cells. CK and vimentin were not suitable for distinguishing between reactive mesothelial cells and carcinoma cells.

• Applied Microscopy
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• v.35 no.2
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• pp.65-72
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• 2005

The ultrastructure of submandibular gland was examined in the lesser white-toothed shrew, Crocidura suaveolens. The submandibular gland of C. suaveolens was a mixed gland composed of serous and mucous acinar cells. Secretory granules from the acini were discharged through the salivary ducts into the oral cavity. Serous and mucous acinar cells had well developed rough endoplasmic reticulum and mitochondria and large amount of granules. In case of serous acinar granules, an immature granule was formless and had only dense specks, and a matured granule was a complete round type delimiting by a single membrane and had a homogeneous dense center with dense specks on the border. In case of mucous acinar granules, while an immature granule was a round type and had an only homogeneous matrix and an indistinct limiting membrane, a mature granule was an even round type having a variety of pattern with several dense bands into the homogeneous matrix and had a distinct membrane. Therefore, a mature mucous acinar granule of C. suaveolens was not only distinct from those of the other mammalian species to have a variety of pattern but also from those of C. lasiura to have an even round type. A great serous-like secretory granules and Myelin-like body were observed in the cytoplasm and lumen of granular duct cells. Myelin-like body, a characteristic structure only reported in salivary gland of three shrews, was discharged from secretory cell into lumen by the manner of exocytosis which has little differences from discharging manner of secretory granules.

• The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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• v.18 no.3
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• pp.95-101
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• 2005

Objective: In children with acute otitis media, we investigated the relationship between state of tympanic membrane and treatment period by video otoscope. Methods: Ten children(6 boys, 4 girls) with acute otitis media were assigned to one of three groups. In the grade I, there were redness in the tympanic membrane. In the grade II, there were bulging, effusion with exudate, dark color in the tympanic membrane. In the grade III, there were effusion with purulent exudate or cholesteatoma with serous exudate. And we investigated the treatment period in each group. Results: Overall, the $mean{\pm}S.D.$ on the treatment period in the grade I was shorter than grade II. It was $12.6{\pm}5.18$ in the grade I versus $26{\pm}2.83$ in the grade II. That in the grade II was shorter than grade III. It was $26{\pm}2.83$ in the grade II versus $148{\pm}43.03$ in the grade III. Conclusions: Although we couldn't have enough cases in each group. we could predict the conclusion that there was the relationship between treatment period and state of tympanic membrane.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• v.28 no.6
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• pp.413-420
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• 2002

Mercury is one of the most frequently used heavy metal in dental clinic. Mercury poisoning rises up when someone is exposed to mercury chronically. In 1818, Amalgam was used for dental restorative procedure, and after then study about mercury toxicity has begun. Clinical signs of mercury toxicity in oral & maxillofacial area were increases of salivation, metallic taste, swelling and pain of tongue, redness and ulceration of oral mucosa, and increased mobility and loss of teeth. After we injected mercury($HgCl_{2}$) into intraperitoneum of rat, studied about histopathological changes of submandibular gland cell. Experimental group was divided into two groups by amount of mercury. (Group 1 was 0.5mg/Kg of mercury injection, group 2 was 1.0mg/Kg of mercury injection.) 1. After 3days of intraperitoneal injection, black granules were observed at macrophage cell in both group. In group 2, author found hyperchromatism of nucleus, and vacuolization of cellular matrix and nucleus of acinar cell. 2. After 1week of intraperitoneal injection, author found severe vacuolization of nucleus and cellular matrix, and irregular granules around nuclear membrane at mucous cell and serous cell in both group. Vacuolization of nucleus and cellular matrix was seen at duct cell in group 2. 3. After 2weeks of intraperitoneal injection, author could found severe vacuolization of cellular matrix, and sometimes nucleus was positioned in central area of cellular matrix at mucous and serous cell in both group. Vacuolization of nucleus and cellular matrix was found at vascular endothelial cell in group 2. 4. After 4weeks of intraperitoneal injection, destruction and distortion of gland cells were distinct. Vacuolization and destruction of nucleus and cellular matrix was found at duct cell in group 2. After intraperitoneal injection of mercury, we found equanimity of mercury and destruction of cellular matrix at serous cell, mucous cell, and duct cell of submandibular gland. So, we thought that metallic taste of mercury poisoning patient would be due to excretion of saliva containing mercury.

• Proceedings of the KOR-BRONCHOESO Conference
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• 1993.05a
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• pp.68-68
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• 1993

Immunohistochemistry combined with electron microscopy was employed to investigate the distribution of substance P -immunoreactive(SP-IR) nerve fibers in the tracheal submucosal gland of cats. SP-IR nerve fibers were found to form network around the glands. Numerous varicosities were also detected within the basement membrane of the acini and secretory tubules. All the intraglandular varicosities showed close spatial contact with serous cells, mucous cells and myoepithelial cells. Our findings suggest that SP-induced mucus secretion from tracheal submucosal glands in cats may be caused not only by glandular contractile response of myoepithelial cells but also by direct stimulation to both serous and mucous cells.

• Journal of Yeungnam Medical Science
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• v.4 no.2
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• pp.185-191
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• 1987

The systemic lupus erythematosus is a self-perpetuating disease with multisystem involvement, ie ; skin, kidney, serous membrane, nervous system and other organs. The mortality in SLE is determined primarily by the extent of renal involvement ana the degree of immunosuppression resulting from the therapy. We experienced two cases of lupus nephritis in SLE with clinical, serologic, immunologic and pathologic evaluations. Renal biopsy revealed focal and segmental proliferative glomerulonephritis and mesangial proliferative glomerulonephritis. Both patients have been improving with prednisolone on follow-up studies.

• Korean Journal of Veterinary Service
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• v.44 no.2
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• pp.113-117
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• 2021

Cocklebur poisoning in livestock can cause sudden death, with clinical signs include depression, inappetite, blindness, reluctance to move, hypersensitivity, ataxia and coma. The cause of cocklebur poisoning is ingestion of cocklebur sprout or seed, which contains carboxyatractyloside. In December 2020, a 47 month-old Hanwoo suddenly developed ataxia, and died after several hours. Hay mixed cocklebur seeds was fed to Hanwoo for 4 days before the symptoms. At autopsy, petechia and ecchymosis were seen on serous membrane of rumen and intestines. Peritoneal cavities contained a yellowish fluid and, hypoglycemia (Glu <20 mg/dL) was measured in blood test result. Microscopic lesions were karyolysis of centriloular hepatocyte and hemorrhage. Based on autopsy, blood and histopathological test, we diagnosed this case as cocklebur poisoning in Hanwoo.

• Pediatric Gastroenterology, Hepatology & Nutrition
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• v.20 no.3
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• pp.198-203
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• 2017

Eosinophilic gastrointestinal disorder (EGID) is a rare disease in children that affects the bowel wall, with eosinophilic infiltration in the absence of any other causes for eosinophilia. The etiology remains unknown, but allergies and immunological imbalance are suspected triggers. We encountered a case of serosal EGID presenting as intractable vomiting and ascites in a 9-year-old girl, after influenza virus infection. Peripheral eosinophilia was not present. The diagnosis was confirmed by biopsy of the bowel wall through laparotomy and endoscopy, and controlled by 2 courses of steroid therapy due to recurring symptoms. Influenza virus infection was assumed to play a role in the onset of EGID through a Th2 response that stimulated eosinophilic infiltration in the GI tract. We therefore report this case along with a literature review.