• 제목/요약/키워드: Serial Section

검색결과 60건 처리시간 0.029초

현미경 섹션 영상으로부터 3차원 형상 복구 기법 (3D Shape Reconstruction from Microscopic Serial Section Images)

  • 윤일동;이후성
    • 대한전자공학회:학술대회논문집
    • /
    • 대한전자공학회 2003년도 하계종합학술대회 논문집 Ⅳ
    • /
    • pp.2379-2382
    • /
    • 2003
  • This paper describes the design, implementation and results of a unified non-rigid image registration method for the purposes of 3D shape reconstruction from serial section images. The proposed method uses active contour-based segmentation and compensation of radial distortion. Experimental results show that multiple images can be segmented and reconstructed by active single contour as well as intra- and inter-section registration.

  • PDF

실리콘 압력 센서의 디지털 보정 회로의 설계 (Design of Digital Calibration Circuit of Silicon Pressure Sensors)

  • 김규철
    • 전기전자학회논문지
    • /
    • 제7권2호
    • /
    • pp.245-252
    • /
    • 2003
  • 디지털 보정 기능을 갖는 CMOS 압력 센서의 인터페이스 회로를 설계하였다. 인터페이스 회로는 아날로그 부분과 디지털 부분으로 구성되어 있다. 아날로그 부분은 센서로부터 발생한 약한 신호를 증폭시키는 역할을 담당하고 디지털 부분은 온도 보상 및 오프셋 보정 기능을 담당하며 센서 칩과 보정을 조정하는 마이크로컨트롤러와의 통신을 담당한다. 디지털 부분은 I2C 직렬 인터페이스, 메모리, 트리밍 레지스터 및 제어기로 구성된다. I2C 직렬 인터페이스는 IO 핀 수 및 실리콘 면적 면에서 실리콘 마이크로 센서의 요구에 맞게 최적화 되었다. 이 설계의 주요 부분은 최적화된 I2C 프로토콜을 구현하는 제어 회로를 설계하는 것이다. 설계된 칩은 IDEC의 MPW를 통하여 제작되었다. 칩의 테스트를 위하여 테스트 보드를 제작하였으며 테스트 결과 예상한대로 디지털 보정기능이 잘 수행됨을 확인하였다.

  • PDF

Reconstruction of Neural Circuits Using Serial Block-Face Scanning Electron Microscopy

  • Kim, Gyu Hyun;Lee, Sang-Hoon;Lee, Kea Joo
    • Applied Microscopy
    • /
    • 제46권2호
    • /
    • pp.100-104
    • /
    • 2016
  • Electron microscopy is currently the only available technique with a spatial resolution sufficient to identify fine neuronal processes and synaptic structures in densely packed neuropil. For large-scale volume reconstruction of neuronal connectivity, serial block-face scanning electron microscopy allows us to acquire thousands of serial images in an automated fashion and reconstruct neural circuits faster by reducing the alignment task. Here we introduce the whole reconstruction procedure of synaptic network in the rat hippocampal CA1 area and discuss technical issues to be resolved for improving image quality and segmentation. Compared to the serial section transmission electron microscopy, serial block-face scanning electron microscopy produced much reliable three-dimensional data sets and accelerated reconstruction by reducing the need of alignment and distortion adjustment. This approach will generate invaluable information on organizational features of our connectomes as well as diverse neurological disorders caused by synaptic impairments.

2차원 단면 데이터로부터 복합 $G^{1}$ 자유곡면 생성 (Composite $G^{1}$ surface construction from 2D cross-sections)

  • 박형준;나상욱;배채열
    • 한국경영과학회:학술대회논문집
    • /
    • 대한산업공학회/한국경영과학회 2004년도 춘계공동학술대회 논문집
    • /
    • pp.44-47
    • /
    • 2004
  • This paper proposes an approach for composite surface reconstruction from 2D serial cross-sections, where the number of contours varies from section to section. In a triangular surface-based approach taken in most reconstruction methods, a triangular $G^{1}$ surface is constructed by stitching triangular patches over a triangular net generated from the compiled contours. In the proposed approach, the resulting surface is a composite $G^{1}$ surface consisting of three kinds of surfaces: skinned, surface is first represented by a B-spline surface approximating the serial contours of the skinned region and then serial contours of the skinned region and then transformed into a mesh of rectangular Bezier patches. On branched and capped regions, triangular $G^{1}$ surfaces are constructed so that the connections between the triangular surfaces and their neighboring surfaces are $G^{1}$ continuous. Since each skinned region is represented by an approximated rectangular $G^{2}$ surface instead of an interpolated triangular $G^{1}$ surface, the proposed approach can provide more visually pleasing surfaces and realize more efficient data reduction than the triangular surface-based approach. Some experimental results demonstrate its usefulness and quality.

  • PDF

Small Target Detecting and Tracking Using Mean Shifter Guided Kalman Filter

  • Ye, Soo-Young;Joo, Jae-Heum;Nam, Ki-Gon
    • Transactions on Electrical and Electronic Materials
    • /
    • 제14권4호
    • /
    • pp.187-192
    • /
    • 2013
  • Because of the importance of small target detection in infrared images, many studies have been carried out in this area. Using a Kalman filter and mean shift algorithm, this study proposes an algorithm to track multiple small moving targets even in cases of target disappearance and appearance in serial infrared images in an environment with many noises. Difference images, which highlight the background images estimated with a background estimation filter from the original images, have a relatively very bright value, which becomes a candidate target area. Multiple target tracking consists of a Kalman filter section (target position prediction) and candidate target classification section (target selection). The system removes error detection from the detection results of candidate targets in still images and associates targets in serial images. The final target detection locations were revised with the mean shift algorithm to have comparatively low tracking location errors and allow for continuous tracking with standard model updating. In the experiment with actual marine infrared serial images, the proposed system was compared with the Kalman filter method and mean shift algorithm. As a result, the proposed system recorded the lowest tracking location errors and ensured stable tracking with no tracking location diffusion.

비접촉 전원장치에 적용한 직.병렬 공진 DC/DC 컨버터에 관한 연구 (A Study on the Serial-Parallel Resonant DC/DC Converter for Contactless Power Supply System)

  • 황계호;이봉섭;김동희
    • 조명전기설비학회논문지
    • /
    • 제22권5호
    • /
    • pp.31-40
    • /
    • 2008
  • 최근 FPD 산업에서 물류이송장비의 전원장치로 비접촉 전원장치에 관한 연구가 활발히 진행되고 있다. 양산 라인에 적용되고 있는 물류장비의 비접촉 전원장치는 주로 직선구간을 왕복하고, 이송대차가 한 대인 경우에 적용하고 있다. 그러나 곡선 구간과 이송대차가 다수인 경우의 물류이송장비에는 적합하지 않다. 따라서 본 논문은 직 곡선 구간이 혼용되어진 구조이고, 이송대차가 한 대 이상으로 구동되는 물류이송장비의 비접촉 전원장치 시작품에 적용하였고, 적용된 비접촉 전원장치의 회로 구조는 풀 브릿지 구조를 갖는 직 병렬 공진 DC/DC 컨버터 회로로 하여 회로해석, 제어방법, 특성평가, 실험을 통한 적용 가능성에 대한 연구를 행하였다. 또한 비접촉 전원장치의 전체 제어는 1차측 전원공급장치에 일정한 공진전류를 제어하기 위해 듀티 제어를 사용하였고, 2차측 전원공급장치의 출력전압을 일정하게 유지하기 위해 다수의 이송대차가 자체 제어를 하도록 구성하였다. 실제 2대의 이송대차를 제작하여 직 곡선 구간을 구성하고, 물류이송장비를 운용하여 양호한 특성으로 적용 가능함을 알 수 있었다.

Structural Analysis of Exosomes Using Different Types of Electron Microscopy

  • Choi, Hyosun;Mun, Ji Young
    • Applied Microscopy
    • /
    • 제47권3호
    • /
    • pp.171-175
    • /
    • 2017
  • Negative staining has been traditionally used for exosome imaging; however, the technique is limited to surface topology only and can cause staining artifacts. Therefore, to analyze the internal structure of exosomes, we employed a method of block preparation, thin sectioning, and electron tomography. In addition, an automatic serial sectioning technique with 15-nm thickness through focused ion beam was employed to observe the three-dimensional structure of exosomes of various sizes. Cryo-transmission electron microscopy revealed the near-to-native structure of exosomes.

Nano-Resolution Connectomics Using Large-Volume Electron Microscopy

  • Kim, Gyu Hyun;Gim, Ja Won;Lee, Kea Joo
    • Applied Microscopy
    • /
    • 제46권4호
    • /
    • pp.171-175
    • /
    • 2016
  • A distinctive neuronal network in the brain is believed to make us unique individuals. Electron microscopy is a valuable tool for examining ultrastructural characteristics of neurons, synapses, and subcellular organelles. A recent technological breakthrough in volume electron microscopy allows large-scale circuit reconstruction of the nervous system with unprecedented detail. Serial-section electron microscopy-previously the domain of specialists-became automated with the advent of innovative systems such as the focused ion beam and serial block-face scanning electron microscopes and the automated tape-collecting ultramicrotome. Further advances in microscopic design and instrumentation are also available, which allow the reconstruction of unprecedentedly large volumes of brain tissue at high speed. The recent introduction of correlative light and electron microscopy will help to identify specific neural circuits associated with behavioral characteristics and revolutionize our understanding of how the brain works.

감성공간디자인의 실증적 연구-II (An Empirical Study on Emotional Space Design-II)

  • 오영근
    • 한국실내디자인학회논문집
    • /
    • 제21권1호
    • /
    • pp.103-110
    • /
    • 2012
  • With a theoretical focus on the emotional experiences created via the interface, and the relationship between human beings and space, this study aims to corroborate and clarify the formation and verification of emotional interactions between human beings and space using body movements. It follows the method of Coining "Movement Phrases" through the analysis of body movements in the experimental space, thereby developing them into a complete scenario to produce the story of emotional expression. This study has hereby generated the following outcomes: First, the "pocket-type" exhibition displays a higher frequency of body movements than the "general" exhibition. It has close connections with emotional vocabularies: "Curious," "interesting," "warm," and "fun." The "general" exhibition records a relatively high frequency of emotional vocabularies like "natural," "efficient," and "free." Second, it is possible to analyze the story of space using a scenario, just like drama, based upon attributes and serial relationships. The "exposition" section reveals a high degree of "curiosity" and a large number of body movements, while the "development" section indicates high degree of "surprise" plus slight body movements. The "transition" sections manifest high "interest" and many body movements, and the "climax" section shows a high frequency of "surprise" and many changes in body movements. The "conclusion" section finally invokes images together with body movements.

  • PDF