• Title/Summary/Keyword: Serial Section

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3D Shape Reconstruction from Microscopic Serial Section Images (현미경 섹션 영상으로부터 3차원 형상 복구 기법)

  • 윤일동;이후성
    • Proceedings of the IEEK Conference
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    • 2003.07e
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    • pp.2379-2382
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    • 2003
  • This paper describes the design, implementation and results of a unified non-rigid image registration method for the purposes of 3D shape reconstruction from serial section images. The proposed method uses active contour-based segmentation and compensation of radial distortion. Experimental results show that multiple images can be segmented and reconstructed by active single contour as well as intra- and inter-section registration.

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Design of Digital Calibration Circuit of Silicon Pressure Sensors (실리콘 압력 센서의 디지털 보정 회로의 설계)

  • Kim, Kyu-Chull
    • Journal of IKEEE
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    • v.7 no.2 s.13
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    • pp.245-252
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    • 2003
  • We designed a silicon pressure sensor interface circuit with digital calibration capability. The interface circuit is composed of an analog section and a digital section. The analog section amplifies the weak signal from the sensor and the digital section handles the calibration function and communication function between the chip and outside microcontroller that controls the calibration. The digital section is composed of I2C serial interface, memory, trimming register and controller. The I2C serial interface is optimized to suit the need of on-chip silicon microsensor in terms of number of IO pins and silicon area. The major part of the design is to build a controller circuit that implements the optimized I2C protocol. The designed chip was fabricated through IDEC's MPW. We also made a test board and the test result showed that the chip performs the digital calibration function very well as expected.

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Reconstruction of Neural Circuits Using Serial Block-Face Scanning Electron Microscopy

  • Kim, Gyu Hyun;Lee, Sang-Hoon;Lee, Kea Joo
    • Applied Microscopy
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    • v.46 no.2
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    • pp.100-104
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    • 2016
  • Electron microscopy is currently the only available technique with a spatial resolution sufficient to identify fine neuronal processes and synaptic structures in densely packed neuropil. For large-scale volume reconstruction of neuronal connectivity, serial block-face scanning electron microscopy allows us to acquire thousands of serial images in an automated fashion and reconstruct neural circuits faster by reducing the alignment task. Here we introduce the whole reconstruction procedure of synaptic network in the rat hippocampal CA1 area and discuss technical issues to be resolved for improving image quality and segmentation. Compared to the serial section transmission electron microscopy, serial block-face scanning electron microscopy produced much reliable three-dimensional data sets and accelerated reconstruction by reducing the need of alignment and distortion adjustment. This approach will generate invaluable information on organizational features of our connectomes as well as diverse neurological disorders caused by synaptic impairments.

Composite $G^{1}$ surface construction from 2D cross-sections (2차원 단면 데이터로부터 복합 $G^{1}$ 자유곡면 생성)

  • Park, Hyung-Jun;Na, Sang-Wook;Bae, Chae-Yeol
    • Proceedings of the Korean Operations and Management Science Society Conference
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    • 2004.05a
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    • pp.44-47
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    • 2004
  • This paper proposes an approach for composite surface reconstruction from 2D serial cross-sections, where the number of contours varies from section to section. In a triangular surface-based approach taken in most reconstruction methods, a triangular $G^{1}$ surface is constructed by stitching triangular patches over a triangular net generated from the compiled contours. In the proposed approach, the resulting surface is a composite $G^{1}$ surface consisting of three kinds of surfaces: skinned, surface is first represented by a B-spline surface approximating the serial contours of the skinned region and then serial contours of the skinned region and then transformed into a mesh of rectangular Bezier patches. On branched and capped regions, triangular $G^{1}$ surfaces are constructed so that the connections between the triangular surfaces and their neighboring surfaces are $G^{1}$ continuous. Since each skinned region is represented by an approximated rectangular $G^{2}$ surface instead of an interpolated triangular $G^{1}$ surface, the proposed approach can provide more visually pleasing surfaces and realize more efficient data reduction than the triangular surface-based approach. Some experimental results demonstrate its usefulness and quality.

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Small Target Detecting and Tracking Using Mean Shifter Guided Kalman Filter

  • Ye, Soo-Young;Joo, Jae-Heum;Nam, Ki-Gon
    • Transactions on Electrical and Electronic Materials
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    • v.14 no.4
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    • pp.187-192
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    • 2013
  • Because of the importance of small target detection in infrared images, many studies have been carried out in this area. Using a Kalman filter and mean shift algorithm, this study proposes an algorithm to track multiple small moving targets even in cases of target disappearance and appearance in serial infrared images in an environment with many noises. Difference images, which highlight the background images estimated with a background estimation filter from the original images, have a relatively very bright value, which becomes a candidate target area. Multiple target tracking consists of a Kalman filter section (target position prediction) and candidate target classification section (target selection). The system removes error detection from the detection results of candidate targets in still images and associates targets in serial images. The final target detection locations were revised with the mean shift algorithm to have comparatively low tracking location errors and allow for continuous tracking with standard model updating. In the experiment with actual marine infrared serial images, the proposed system was compared with the Kalman filter method and mean shift algorithm. As a result, the proposed system recorded the lowest tracking location errors and ensured stable tracking with no tracking location diffusion.

A Study on the Serial-Parallel Resonant DC/DC Converter for Contactless Power Supply System (비접촉 전원장치에 적용한 직.병렬 공진 DC/DC 컨버터에 관한 연구)

  • Hwang, Gye-Ho;Lee, Bong-Sub;Kim, Dong-Hee
    • Journal of the Korean Institute of Illuminating and Electrical Installation Engineers
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    • v.22 no.5
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    • pp.31-40
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    • 2008
  • Recently, Contactless Power Supply(CPS) system has been broadly studied as a power supply system for Flat Panel Display(FPD) material transfer equipments. In mass production line, CPS systems on material transfer equipment are applied only in the straight sections with single operating vehicle. The formal CPS system was not adequate for curved section nor multiple operating vehicles. Therefore, this paper presents CPS system that consists of straight and curved section with multiple operation vehicles. The circuit topology of CPS system consists of full bridge configured serial-parallel resonant DC/DC converter. The control method for CPS system consists of duty control method on the primary power supply system to maintain constant resonant current. And the secondary power supply systems of multiple vehicles are self controled to maintain constant output voltage. Practically, the test result of dual vehicles on straight and curved section of material transfer equipments were satisfactory, and proved it's applicability on commercial use.

Structural Analysis of Exosomes Using Different Types of Electron Microscopy

  • Choi, Hyosun;Mun, Ji Young
    • Applied Microscopy
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    • v.47 no.3
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    • pp.171-175
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    • 2017
  • Negative staining has been traditionally used for exosome imaging; however, the technique is limited to surface topology only and can cause staining artifacts. Therefore, to analyze the internal structure of exosomes, we employed a method of block preparation, thin sectioning, and electron tomography. In addition, an automatic serial sectioning technique with 15-nm thickness through focused ion beam was employed to observe the three-dimensional structure of exosomes of various sizes. Cryo-transmission electron microscopy revealed the near-to-native structure of exosomes.

Nano-Resolution Connectomics Using Large-Volume Electron Microscopy

  • Kim, Gyu Hyun;Gim, Ja Won;Lee, Kea Joo
    • Applied Microscopy
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    • v.46 no.4
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    • pp.171-175
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    • 2016
  • A distinctive neuronal network in the brain is believed to make us unique individuals. Electron microscopy is a valuable tool for examining ultrastructural characteristics of neurons, synapses, and subcellular organelles. A recent technological breakthrough in volume electron microscopy allows large-scale circuit reconstruction of the nervous system with unprecedented detail. Serial-section electron microscopy-previously the domain of specialists-became automated with the advent of innovative systems such as the focused ion beam and serial block-face scanning electron microscopes and the automated tape-collecting ultramicrotome. Further advances in microscopic design and instrumentation are also available, which allow the reconstruction of unprecedentedly large volumes of brain tissue at high speed. The recent introduction of correlative light and electron microscopy will help to identify specific neural circuits associated with behavioral characteristics and revolutionize our understanding of how the brain works.

An Empirical Study on Emotional Space Design-II (감성공간디자인의 실증적 연구-II)

  • Oh, Young-Keun
    • Korean Institute of Interior Design Journal
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    • v.21 no.1
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    • pp.103-110
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    • 2012
  • With a theoretical focus on the emotional experiences created via the interface, and the relationship between human beings and space, this study aims to corroborate and clarify the formation and verification of emotional interactions between human beings and space using body movements. It follows the method of Coining "Movement Phrases" through the analysis of body movements in the experimental space, thereby developing them into a complete scenario to produce the story of emotional expression. This study has hereby generated the following outcomes: First, the "pocket-type" exhibition displays a higher frequency of body movements than the "general" exhibition. It has close connections with emotional vocabularies: "Curious," "interesting," "warm," and "fun." The "general" exhibition records a relatively high frequency of emotional vocabularies like "natural," "efficient," and "free." Second, it is possible to analyze the story of space using a scenario, just like drama, based upon attributes and serial relationships. The "exposition" section reveals a high degree of "curiosity" and a large number of body movements, while the "development" section indicates high degree of "surprise" plus slight body movements. The "transition" sections manifest high "interest" and many body movements, and the "climax" section shows a high frequency of "surprise" and many changes in body movements. The "conclusion" section finally invokes images together with body movements.

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