• 제어로봇시스템학회논문지
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• 제6권5호
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• pp.415-425
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• 2000

An open architecture manufacturing strategy intends to integrate manufacturing components on a single platform so that a particular component can be easily added and/or replaced. Therefore, the control scheme based upon the open architecture concept is hardware-independent. In this paper, a modular and object oriented approach for a PC-based open robot control system is investigated. A standard reference model for robot systems, which consists of three modules; hardware module, operating system module, and application software module, is first proposed. Then, a PC-based Open Robot Controller(PC-ORC), which can reconfigure robot control systems in various production environments, is developed. The PC-ORC is built upon the object-oriented method, and allows an easy implementation and modification of various modules. The PC-ORC consists of basic softwares, application objects, and additional hardware device on the PC Platform. The application objects are: sequencer, computation unit, servo control, ancillary equipment, external sensor control, and so on. In order to demonstrate the applicability of the PC-ORC, the proposed PC-ORC configuration is applied to an industrial SCARA robot system.

• 한국수산과학회지
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• 제28권6호
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• pp.728-735
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• 1995

The peptidyl prolyl cis-trans isomerase(PPlase, EC 5.2.2.8) from Bacillus stearothermophilus SIC1 was extracted from the cells treated with by lysozyme. PPlase was purified from the cell extracts by heat treatment, ammonium sulfate precipitation, ion exchange chromatography and finally gel filtration (FPLC). The purity of purified the enzyme after Superose 12 column chromatography was examined by sodium dodecyl sulfate polyacrylamide gel electrophoresis(SDS-PAGE). The molecular weight of the purified PPlase was estimated as 18,000 by SDS-PAGE. The 39 amino acid residues from the N-terminus were determined by the protein sequencer. The enzyme showed the optimum pH at 8.0 and was stable at the range of pH 7.0 to 8.0. The enzyme was considerably stable after heat treatment at $60^{\circ}C$ for 30 minutes, and the enzyme was quite stable up to $65^{\circ}C$. The presence of the PPlase in the refolding solution accelerated the isomerization rate of the assay peptide.

• 대한한의학방제학회지
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• 제19권2호
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• pp.83-92
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• 2011

Objectives : This study was carried out to moniter microbial flora on freeze-dried herbal powder and identify isolated bacteria. Methods : We measured the total number of bacteria and fungi in 29 herbal powder which had made according to the guideline of KFDA. For the identification, we observed microscopic properties and carried out polymerase chain reaction(PCR). The purified DNA was analyzed by DNA sequencer. Results : Among the 29 herbal powders, the fungi were detected only one sample as unacceptable range of total aerobic bacteria. Isolated bacteria were identified as Bacillus cereus, B. subtilis, B. megaterium, B. licheniformis, Erwinia tasmaniensis, E. amylovora, and Pantoea agglomerans by 16S rDNA analysis. E. tasmaniensis was observed 20 herbal samples. Conclusions : According to above results, further studies for the effective sterilization of low herbal materials should be needed.

• 한국진공학회:학술대회논문집
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• 한국진공학회 2011년도 제40회 동계학술대회 초록집
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• pp.237-237
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• 2011

다가 이온원 제조를 위한 ECR 이온원 제작과 더불어 이를 효과적으로 운전 제어할 수 있는 방안을 만들어야 한다. 이 운전 제어 방안은 두 가지 기능을 만족할 수 있도록 구성해야 하는데, 초기 실험실에서 ECR 이온원의 성능 검증을 하기에 유리하도록 알고리즘이나 시퀀스를 보다 용이하게 변경할 수 있도록 만들고 이후 확정된 알고리즘과 시퀀스를 이용하여 이온원의 안전을 보장하면서 최소한의 운전자만으로도 운전 가능하도록 구성해야 한다. 이를 구현하기 위해서 ECR 이온원 운전 제어와 관계된 모든 부대 장치들을 EPICS (Experimental Physics and Industrial Control System)로 운전하고 제어할 수 있도록 통합하면서 알고리즘의 복잡함으로 인해 발생할 수 있는 불확실성을 줄이기 위해 부대 장치를 구성하는 개별 장치 단위로 알고리즘을 만들어 EPICS database로 구현하고 운전 및 장치 보호를 위해 필요한 시퀀스는 EPICS sequencer를 이용하여 구현하였다. 현재까지 ECR 플라즈마 생성, 빔인출 그리고 입자 진단을 위해 사용된 전원 장치들을 운전 제어하기 위해 구현된 내용을 소개하고 이를 바탕으로 개별 장치에 확대하여 적용할 수 있는 방안에 대하여 연구하였다.

• 분석과학
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• 제8권4호
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• pp.465-468
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• 1995

Matrix assisted laser desorption ionization in mass spectrometry is a fast and accurate method to determine the molecular weight of natural and synthetic polymers. Unknown peptides such as elastase inhibitor and $\small{D}$-hydantoinase were analyzed using sinapinic acid as matrix and their molecular weights were compared with the results from protein sequencer and gel filtration chomatography, respectively. Synthetic polymers such as polyethyleneglycol, polypropyleneglycol, polydimethylsiloxane, and polystyrene were analyzed using matrices such as 2,5-dihydroxybenzoic acid, 4-hdroxyazobenzenecarboxylic acid, and 2-nitrophenyl octyl ether. Average molecular weights of polystyrene were compared with molecular weights by gel permeation chromatography.

• 보존과학연구
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• 통권24호
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• pp.153-167
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• 2003

We performed nuclear DNA typing and mitochondrial DNA sequencing analysis based on PCR from an ancient Korean remainsexcavated from Siheung in Korea. 7 bones were collected and partially STR(short tandem repeat) systems, Sex determination Amelogenin kit(Promega co, USA), were used in this study. Mitochondrial DNAs were also amplified and sequenced by ABI 310 DNA sequencer. We know that sample no. 2 and no. 3 were females and also sample no. 2 and no.7 possessed the same maternal inheritance by mitochondrial DNA sequencing results. Throughout this research, the mitochondrial DNA sequencing of human in the middle of Joseon Dynasty in Korea is obtained. In addition, this finding will be an important foundation for the future research.

• Preventive Nutrition and Food Science
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• 제4권2호
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• pp.139-144
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• 1999

Lipoprotein lipase (LPL) I san enzyme that catalyzed the hydrolysis of triacylglycerols of chylomicrons and VLDL to produce 20acylglycerols and fatty acids. The enzyme, LPL, is localized on the surface of the capillary endothelium and is widely distributed in extrahepatic tissues including heart, skeletal muscle and adipose tissue. LPL has been isolated from boving milk by affinity chromatography on heparin-separose in 2 M NaCL, 5mM barbital buffer, pH 7.4. To elucidate the lipid-binding regin, LPL was digested with trypsin and then separated by gel filtration. Lipid binding region of LPL has been investigated by recombining LPL peptides with DMPC vesicles. Proteolytic LPL fragments with DMPC were reassembled and stabilized by cholate. Lipid-binding region of LPL was identified by a PTH-automated protein sequencer, as AQQHYPVSAGYTK. The analysis of the secondary structure of the lipid-binding peptides revealed a higher probability of $\alpha$-helix structure compared to the whole LPL protein. The prediction of hydrophobicity of lipid -binding region was highly hydrophobic (-1.1) compared to LPL polypetide(-0.4).

• 대한전기학회:학술대회논문집
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• 대한전기학회 1996년도 하계학술대회 논문집 B
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• pp.1164-1166
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• 1996

This system is an unified Digital Generator Controller. It has an Electronical Generator Sequencer which control the sequence of a generator, an AVR(Automatic Voltage Regulator) which perform the regulation of generator terminal voltage optimally to user's needs, and an Auto Synchronizer, it can switch the power source on from generally used an the generator to load automatically. It also possesses a Battery Charger which charge a storage battery to appropriate voltage level. Here we describe it's configuration, specifications, the way of control, figure, functions and waveforms.

• 한국통신학회논문지
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• 제35권6B호
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• pp.954-961
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• 2010

스트림 암호는 블록 암호보다 안전성은 떨어지지만 수행 속도가 빠른 것이 큰 장점이었다. 그러나 최근까지 블록 암호의 수행 속도를 개선한 알고리즘 개발로 지금은 AES의 경우 스트림 암호와 수행 속도 차가 거의 없게 되어, 안전하면서 빠른 스트림 암호 개발이 절실히 요구된다. 본 논문에서는 ASR(Arithmetic Shift Register)과 간단한 논리연산으로 구성된 32비트 출력의 고속 스트림 암호 AA32를 제안한다. 제안한 알고리즘은 소프트웨어 구현이 쉽게 디자인된 스트림 암호 알고리즘으로 128비트 키를 지원하고 있으며, 워드와 바이트 단위로 연산을 수행한다. AA32의 전체 구성은 선형 궤환 순서기(Linear Feedback Sequencer)로 ASR 151비트를 적용하였고, 축소함수는 비선형(Non-Linear) 연산을 위한 S-박스를 사용하지 않고 간단한 논리연산을 사용한 크게 두 부분으로 구성되어 있는 매우 간결한 구조의 스트림 암호이다. 제안한 스트림 암호 AA32는 SSC2, Salsa20 보다 수행 속도 테스트결과 빠른 결과를 보여주고 있으며, 안전성 또한 현대 암호 알고리즘이 필요로 하는 안전성을 만족하고 있다. 제안한 암호 알고리즘은 휴대폰과 같은 무선 인터넷 환경과 DRM(Digital Right Management) 등과 같은 실시간 처리가 필요한 분야와 제한된 환경인 무선 센서 네트워크(Wireless Sensor Network)에 사용 가능한 고속 스트림 암호 알고리즘이다.

• 한국환경성돌연변이발암원학회지
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• 제26권1호
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• pp.20-24
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• 2006

식용 달팽이 (Achatina fulica])의 추출물 RM 60을 사용하여 E. coli D31을 대상으로 순수한 항균성 물질을 분리 정제하였다. 정제한 항균성 물질은 MALDI-TOF Mass spectrometra를 사용하여 분자량을 측정한 결과, 1392.64 Da 단일 peak를 얻을 수 있었으며, 이 후 Edman 분해법을 이용한 peptide sequencer를 사용하여 일차구조 분석을 조사하고 있다.