• BMB Reports
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• v.32 no.5
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• pp.436-439
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• 1999

Shigella sonnei is an important cause of human enteric infections. S. sonnei KNIH104S was previously reported to be isolated from Korean shigellosis patients. We cloned a 2.8-kb KpnI fragment containing the recA gene encoding a recombinase from the chromosomal DNA of S. sonnei KNIH104S. This recombinant plasmid was named pRAK28. E. coli HB101, a recA mutant, cannot grow on Luria-Bertani medium in the presence of the alkylating agent methylmethane sulfonate, however, E. coli HB101 harboring pRAK28 was found to grow on this medium. As far as we know, we are the first to sequence the recA gene from S. sonnei. This gene is composed of 1062 base pairs with an ATG initiation codon and a TAA termination codon. Nucleotide sequence comparison of the S. sonnei recA gene exhibited 99.7% and 99.5% identity with those of S. flexneri and E. coli, respectively.

• Journal of Plant Biotechnology
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• v.3 no.3
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• pp.131-134
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• 2001

In $\lambda$-Zap II vector system, a cDNA library was constructed for the developing secondary xylem mRNA from hybrid poplar, Populus nigra x maximowiczii. A cDNA clone of 1.5 kb in size, pOMTB1.4 encoding a lignin-bispecific O-methyltransferase was screened by plaque hybridization using a probe of 540 bp cDNA amplified by polymerase chain reaction from the cDNA library and identified by nucleotide sequencing. Its nucleotide sequence contains one open reading frame of 366 amino acids. The deduced amino acid sequence in comparison with that of Populus tremuloides showed the differences of 9 amino acids and revealed 85-99% homology among alfalfa, poplar and aspen.

• Asian-Australasian Journal of Animal Sciences
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• v.6 no.4
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• pp.541-547
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• 1993

A phage clone containing the partial ${\alpha}_{S1}$-casein gene was isolated from a bovine genomic library by using mixed probes of ovine ${\alpha}_{S1}$-, ${\beta}$- and ${\kappa}$-casein cDNAs. Restriction enzyme mapping analysis for 14.6 kb revealed that the map was in conflict with the report of Meade et al. (1990), especially in the 3'-end fragment. Sequence analysis of 12.6 kb revealed a high AT/GC ratio (1.64); we have identified eight exon sequences according to the bovine ${\alpha}_{S1}$-casein cDNA sequence. The same exon/intron splice junction sequence was observed between these exons. We suggest that the bovine ${\alpha}_{S1}$-casein gene night contain a minimum of 18 exons and the full length is approximately 18-19 kb.

• Journal of Applied Biological Chemistry
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• v.48 no.4
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• pp.218-221
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• 2005

Nucleotide sequence of Brevibacterium flavum ptsG gene capable of complementing Escherichia coli ZSC113 mutations defective to glucose permease activity of phosphotransferase system was completely determined, and the gene product was compared with other glucose-specific enzyme II ($EII^{Glc}$). A ptsG gene of B. flavum consisted of open reading frame of 2,025 nucleotides putatively encoding polypeptide of 675 amino acid residues and TAA stop codon. Deduced amino acid sequence of B. flavum ($EII^{Glc}$) had high homology with ($EIIs^{Glc}$) of Corynebacterium glutamicum, C. efficiens, and B. lactofermentum. Arrangement of structural domains, IIBCA, of B. flanum ($EII^{Glc}$) protein was identical to that of EIIs belonging to glucose-phosphotransferase system.

• International Journal of Control, Automation, and Systems
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• v.1 no.4
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• pp.444-452
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• 2003

An integration system with multi-measurement sets can be realized via combined application of a centralized and federated Kalman filter. It is difficult for the centralized Kalman filter to remove a failed sensor in comparison with the federated Kalman filter. All varieties of Kalman filters monitor innovation sequence (residual) for detection and isolation of a failed sensor. The innovation sequence, which is selected as an indicator of real time estimation error plays an important role in adaptive mechanism design. In this study, the centralized Kalman filter with adaptive measurement fusion is introduced by means of innovation sequence. The objectives of adaptive measurement fusion are automatic isolation and recovery of some sensor failures as well as inherent monitoring capability. The proposed adaptive filter is applied to the GPS/SDINS integration system with an additional sensor. Simulation studies attest that the proposed adaptive scheme is effective for isolation and recovery of immediate sensor failures.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2002.11b
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• pp.54-54
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• 2002

The nucleotide sequence of a Korea isolate of broad bean wilt favavirus from Rehmannia glutinosa Lib., designated BBWV-RE, was determined. Direct amino acid sequencing of the virus coat proteins suggests that a comparison of several favaviruses in terms of nucleotide sequence and amino acid sequences showed that BBWV-2 isolates display high sequence identity. The small coat protein genes of RNA-2 were also determined for three other Japanese isolates(E, L, and 1-2) and two ATCC isolates(PV132 and PV176) of BBWV. the CP sequence suggested distinct evolution lineages. Serotype 2 favaviruses are more prevalent in Asia, Australia and North America, Wheres serotype 1 is more prevalent in europe.(중략)

• Journal of Industrial Technology
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• v.19
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• pp.217-226
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• 1999

A single phase to three phase power converter with cost effective and simple structure is proposed. The converter consists of rectifier and inverter. The rectifier is composed of a half wave rectifier, a dc link capacitor, and a current limiting inductor, and the inverter is of only two switches with PWM control. For negative sequence operation the inverter output voltage leads the line input by $60^{\circ}$, and for positive sequence operation the inverter output voltage leads by $60^{\circ}$. We can see that positive sequence operation shows higher output voltage, slight harmonic distortion(2%), and better performances such as high efficiency and high power factor. A mathematical model for system analysis is provided, and specifications for selection and control scheme both for start-up and for steady state are analyzed. comparison and operational limits of positive and negative sequence operation are performed, and simulations and experiments are executed to verify the proposed.

• Proceedings of the KSMRM Conference
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• 2003.10a
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• pp.22-22
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• 2003

The purpose of this study was to compare the detection rate of brain metastasis according to size of nodule between 1.5T and 3.0T MRI 대상 및 방법： We reviewed 44 patients with primary tumors and clinical symptoms suggesting brain metastasis. After administration of double dose gadolinium-DTPA, MR imaging was performed with 3D SPGR sequence by 3.0T MRI and then with T1 SE sequence by 1.5T MRI. Consequently, comparison was done in 1.5T T1 SE sequence and 3.0T 3D SPGR sequence. With use of the signal intensity (SI) measurements in the metastatic nodules and adjacent tissue, metastatic nodule-to-adjacent tissue SI ratio were calculated. In each patient, the number of metastatic lesions detected in 1.5T and 3.0T, and their size were assessed qualitatively by three blinded readers.

• Nuclear Engineering and Technology
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• v.53 no.12
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• pp.4080-4092
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• 2021

In this study, the performance of sequence tracking methods for multiple interaction events in specific CdZnTe Compton imagers was evaluated using Monte Carlo simulations. The Compton imager consisted of a 6 × 6 array of virtual Frisch-grid CZT crystals, where the dimensions of each crystal were 5 × 5 × 12 mm³. The sequence tracking methods for another Compton imager that consists of two identical CZT crystals arrays were also evaluated. When 662 keV radiation was incident on the detectors, the percentages of the correct sequences determined by the simple comparison and deterministic methods for two sequential interactions were identical (~80%), while those evaluated using the minimum squared difference method (55-59%) and Three Compton method (45-55%) for three sequential interactions, differed from each other. The reconstructed images of a 662 keV point source detected using single and double arrays were evaluated based on their angular resolution and signal-to-noise ratio, and the results showed that the double arrays outperformed single arrays.

• Journal of Korean Elementary Science Education
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• v.31 no.2
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• pp.164-176
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• 2012

The purpose of this study was to find some suggestions for reorganization of contents about particle concept of matter in Korean science curriculum. For the purpose of this study, authors analyzed features of Korean science curriculum and compared science curricula of Korea, USA, UK, Japan and Finland. From the result of this study, authors find some features and important suggestions about reorganization of contents about particle in science curriculum. First, the sequence of contents about particle concepts in 2009 Revised National Curriculum was similar to that in the 6th National Science Curriculum. And the feature of 2009 Revised National Curriculum showed the articulation of contents about particle concept. If contents about particle concept is increased in elementary science curriculum, the total articulation would be increased. Second, the presenting sequence of atomic structure-first and laws about atom-later should be changed to laws about atomic-first and atomic structure-later. This presenting sequence is grounded by science curricula of other countries, history of science and developmental psychology. And science curriculum of Korea was required specific extended concept statement like science curricula of USA or UK. Also, Korean science curriculum could benchmark Finnish science curriculum if we want to develop some integrated learning activities such as those in STS or STEAM program.