• 약학회지
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• 제34권1호
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• pp.11-14
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• 1990

$NH_2-terminal$ amino acid sequence analysis of monoclonal antibody is very important to identify gene family and diversities of antigen-antibody recognition. When we used the PVDF (Polyvinylidene difluoride) membrane blotting method, we could easily analyze $NH_2-terminal$ sequence of monoclonal-antibody which specifically binds to phosphatidylinositol 4,5-biphosphate. PVDF membrane is an ideal solid-phase support for sequence analysis, especially when used with electroblotting method. This method is superior to continual method and will be applied to the sequence analysis of picomole quantities of proteins by gel electrophoresis.

• 정보과학회 논문지
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• 제44권1호
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• pp.57-62
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• 2017

기존의 전통적인 한국어 형태소 분석 및 품사 태깅 방법론은 먼저 형태소 후보들을 생성한 뒤 수많은 조합에서 최적의 확률을 가지는 품사 태깅 결과를 구하는 두 단계를 거치며 추가적으로 형태소의 접속 사전, 기분석 사전 및 원형복원 사전 등을 필요로 한다. 본 연구는 기존의 두 단계 방법론에서 벗어나 심층학습 모델의 일종인 sequence-to-sequence 모델을 이용하여 한국어 형태소 분석 및 품사 태깅을 추가 언어자원에 의존하지 않는 end-to-end 방식으로 접근하였다. 또한 형태소 분석 및 품사 태깅 과정은 어순변화가 일어나지 않는 특수한 시퀀스 변환과정이라는 점을 반영하여 음성인식분야에서 주로 사용되는 합성곱 자질을 이용하였다. 세종말뭉치에 대한 실험결과 합성곱 자질을 사용하지 않을 경우 97.15%의 형태소 단위 f1-score, 95.33%의 어절단위 정확도, 60.62%의 문장단위 정확도를 보여주었고, 합성곱 자질을 사용할 경우 96.91%의 형태소 단위 f1-score, 95.40%의 어절단위 정확도, 60.62%의 문장단위 정확도를 보여주었다.

• BMB Reports
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• 제30권1호
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• pp.41-45
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• 1997

The present study was performed to analyze the molecular mechanism which dictates the transcription regulation of the $O^6$-methylguanine-DNA methyltransferase (MGMT) gene in Saccharomyces cerevisiae. Previously we identified one possible upstream repressing sequence (URS) in MGMT promoter by promoter deletion and competition analysis. In this paper we report another regulatory element (UAS: upstream activating sequence. -213 to -136) which affects the transcription activity of MGMT promoter. Gel mobility shift assay and Southwestern blot analysis using UAS probe showed several specific proteins which were able to bind to this sequence.

• 한국조경학회지
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• 제19권3호
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• pp.55-71
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• 1991

The purpose of this thesis is to suggest objective basic data for the environmental design through the quantitative analysis of the visual quality included in the physical environment of Basemsagol valley sequence landscape. For this, visual volumes of physical elements have been evaluated by using the mesh analysis, spatial images structure of physical elements have been analyzed by factor analysis algorithm, and degree of visual quality have been measured mainly by questionnaires. Also, this study aims to understand semiotics and to grope the possibility of application to the sequence landscape assessment. A semiological approach suggests a new dimension in sequence landscape assessment, which is a contrast to the existing scientific evaluation methods. Result of this thesis can be summarized as follows. Visual volumes of the immediate vegetation, rock, bridge, road and distant vegetation are found to be the main factor determining the visual quality. Factors covering the spatial image of natural park sequence landscape have been found to be the overall synthetic evaluation, potentiality, natural quality, spatial, appeal and dignity. By using the control method for the number of factors, T.V. has been obtained as 40.22%. The characteristics of the semiological approach is qualitative, open, holistic, and experiential, whereas that of the scientific approach is quantitative, closed, reductive, and experimental.

• Geomechanics and Engineering
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• 제13권5호
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• pp.845-860
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• 2017

It is very important to study displacement prediction in geotechnical engineering. Nowadays, the grey system method, time series analysis method and artificial neural network method are three main methods. Based on the brief introduction, the three methods are analyzed comprehensively. Their merits and demerits, applied ranges are revealed. To solve the shortcomings of the artificial neural network method, a new prediction method based on new evolutionary neural network is proposed. Finally, through two real engineering applications, the analysis of three main methods and the new evolutionary neural network method all have been verified. The results show that, the grey system method is a kind of exponential approximation to displacement sequence, and time series analysis is linear autoregression approximation, while artificial neural network is nonlinear autoregression approximation. Thus, the grey system method can suitably analyze the sequence, which has the exponential law, the time series method can suitably analyze the random sequence and the neural network method almostly can be applied in any sequences. Moreover, the prediction results of new evolutionary neural network method is the best, and its approximation sequence and the generalization prediction sequence are all coincided with the real displacement sequence well. Thus, the new evolutionary neural network method is an acceptable method to predict the measurement displacements of geotechnical engineering.

• ALGAE
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• 제20권1호
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• pp.25-30
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• 2005

To clarify some confusions concerning identification of the Korean Peridinium species, genotypic analysis was performed with their SSU rDNA sequences. PCR was used to amplify the partial SSU rDNA of Peridinium isolates collected from three different Korean waters (Juam, Sang-sa and Togyo Reservoirs). The PCR products were allowed directly to sequence, which revealed each 942 bp of rDNA sequence. Analyses of the rDNA sequences showed that all the Korean isolates had the same genotype (100% sequence homology), and they were nearly identical to a Japanese strain of P. bipes f. occultatum (NIES 364; 99.8% sequence similarity). The sequence-based comparisons could clearly resolve P. bipes f. occultatum isolated from three different Korean waters.

• 한국식물병리학회지
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• 제11권1호
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• pp.87-90
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• 1995

Complementary DNA of the movement protein (MP) gene of tobacco mosaic virus Korean pepper strain (TMV-KP) was synthesized from purified TMV-KP RNA by using the reverse transcription and polymerase chain reaction (PCR) system. The synthesized double stranded cDNA was cloned into the plasmid pUC9 and transformed into Escherichia coli JM110. The movement protein gene of TMV-KP of the selected clones was subjected to sequence analysis by Sanger's dideoxy chain termination method. The complete sequence of viral MP gene from TMV-KP strain was 807 nucleotides long. The nucleotide of MP gene from TMV-KP has thirteen and two nucleotide differences from TMV vulgarae (TMV-OM) and Korean (TMV-K) strains, respectively. Thus, the nucleotide sequence of TMV-KP MP gene showed higher homology of 99% with that of TMV-K MP gene.

• Journal of Welding and Joining
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• 제31권3호
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• pp.54-59
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• 2013

Several methodologies analyzing welding distortions of large shell structures like ship blocks have been developed and utilized in shipyards for a long times ago. In general, one of objects of thermal distortion analysis is to find welding sequence making least-deformation without any suppliance, and it can be solved easily and rapidly by EP strain-boundary method. But after assembly construction, there are usually more than 10 weldments in each process, and the number of sequence will follow the Factorial calculation. In this research, a method has been suggested to decide the best welding sequence by minimum analysis. Using this method, welding deformation could be reduced just analyzing some cases as many as the number of weldments. Experiments and Analysis of all cases were also done, and their best results are good agreements with predictation by suggested methodology.

• 한국가스학회지
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• 제5권1호
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• pp.21-28
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• 2001

회분식 공정의 HAZOP 분석 전문가 시스템은 연속 공정의 HAZOP 분석 기법과는 달리 time과 sequence라는 이산적인 변수들을 고려해야 한다. 따라서 본 연구에서는 이산 변수들과 연속 변수들의 관계를 이용하여 회분식 공정의 time과 sequence 해석을 수행하는 회분식 공정의 HAZOP 분석 자동화를 위한 전문가 시스템을 개발하였다. 제안된 전문가 시스템을 Latex 제조 공정에 적용하여 그 실효성을 검토하였다.

• Journal of Yeungnam Medical Science
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• 제19권1호
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• pp.1-10
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• 2002

With the establishment of rapid sequence analysis of 16S rRNA and the recognition of its potential to determine the phylogenetic position of any prokaryotic organism, the role of 16S rRNA similarities in the present species definition in bacteriology need to be clarified. Comparative studies clearly reveal the limitations of the sequence analysis of this conserved gene and gene product in the determination of relationship at the pathogenic strain level for which DNA-DNA reassociation experiments still constitute the superior method. Since today the primary structure of 16S rRNA is easier to determine than hybridization between DNA strands, the strength of the sequence analysis is to recognize the level at which DNA pairing studies need to be performed, which certainly applies to similarities of 97% and higher.