• Title/Summary/Keyword: Sequence Analyses

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Identification and Characterization of Gonatobotryum apiculatum Causing Leaf Spot and Blight on Sinowilsonia henryi

  • Gao, Ying;Liu, Hai Feng;Song, Zheng Xing;Du, Xiao Ying;Deng, Jian Xin
    • Mycobiology
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    • v.48 no.1
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    • pp.70-74
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    • 2020
  • Sinowilsonia henryi is a rare and endangered plant, as well as an endemic species in China. In July 2018, leaf spot and blight disease was observed on S. henryi in Yichang, Hubei, China. A fungus isolated from disease tissues was identified as Gonatobotryum apiculatum based on morphology and sequence analyses of ITS and LSU regions. Phylogenetic analyses indicated that the species belongs to Dothioraceae (Dothideales). Morphologically, the species produced two distinct types of conidia from authentic media, both conidia were described here. Pathogenicity tests showed that the fungus is a pathogen causing leaf spots on S. henryi. This is the first report of leaf spot and blight disease on S. henryi caused by G. apiculatum in China.

Petrology and Petrochemistry of Pajoo Acidic Igneous pluton (파주(波州) 산성화성암체(酸性火成岩體)의 암석학적(岩石學的) 연구(硏究))

  • Oh, Mihn Soo
    • Economic and Environmental Geology
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    • v.11 no.1
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    • pp.1-9
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    • 1978
  • The study is on petrology and petrochemistry of Pajoo Acidic Igneous pluton which intruded into Precambrian metasediments of basement of the area. The geologic sequence of studied area was shown in table 1 and 10 modal analyses and 7 chemical analyses on the rock samples taken from the body. Pajoo Acidic Igneous rock consist of hypersthene-quartz-diorite and porphyritic adamellite which based on the classification of the subcommision on systematics of igneous rocks of IUGS. And porphyritic adamellite which occured as a small stock was intruded into hypersthene quartz diorite. The rock forming minerals of hypersthene quartz diorite are composed of plagioclase, perthite, quartz, hypersthene, hornblende, biotite and porphyritic adamellite is composed of perthite, quartz, plagioclase and biotite. And the former is hypidiomorphic granular texture and later is porphyritic texture with microcline phenocrysts. In silica-oxides variation diagram, the Pajoo acidic igneous rocks are similar to the trend of Daly's average composition and equivalent to the calc-alkalic rock series. In AMF diagram, these rocks are stock of fissure filling vein type by cooling in magmatic differentiation.

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Vibration and stability analyses of thick anisotropic composite plates by finite strip method

  • Akhras, G.;Cheung, M.S.;Li, W.
    • Structural Engineering and Mechanics
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    • v.3 no.1
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    • pp.49-60
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    • 1995
  • In the present study, a finite strip method for the vibration and stability analyses of anisotropic laminated composite plates is developed according to the higher-order shear deformation theory. This theory accounts for the parabolic distribution of the transverse shear strains through the thickness of the plate and for zero transverse shear stresses on the plate surfaces. In comparison with the finite strip method based on the first-order shear deformation theory, the present method gives improved results for very thick plates while using approximately the same number of degrees of freedom. It also eliminates the need for shear correction factors in calculating the transverse shear stiffness. A number of numerical examples are presented to show the effect of aspect ratio, length-to-thickness ratio, number of plies, fibre orientation and stacking sequence on the natural frequencies and critical buckling loads of simply supported rectangular cross-ply and arbitrary angle-ply composite laminates.

Environmental Gradient Analysis of Forest Vegetation of Mt. Naejang, Southwestern Korea (내장산 삼림식생의 환경경도분석)

  • 김정언
    • Journal of Plant Biology
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    • v.31 no.1
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    • pp.33-39
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    • 1988
  • The environmental gradient analyses were aplied for the ordination of forest vegetation in Mt. Naejang national park area in Korea. The species population sequence along soil moisture gradient, mesic to xeric, was shown in following order: Zelkova serrata, Celtis sinensis, Lindera erythrocarpa, Cornus controversa, Acer mono, Carpinus tschonoskii, Quercus aliena, Daphniphyllum macropodum, Torreya mucifera, Carpinus laxiflora, Quercus serrata, Quercus variabilis, Quercus mongolica and Pinus densiflora in tree species and Acer pseudo-siebolidianum var. koreanum, Lindera obtusiloba, Styrax obassia, Styrax japonica, Acer pseudo-sieboldianum and Rhododendron schlippenbachii in shrub species. Ten ecological groups of tree were grouped and coincided with the vegetational units in phytosociological classification by Z-M method, associations. Four vegetation types, cove forest with Zelkova serrata and Lindera erythrocarpa, hornbeam forest with Carpinum laxiflora and Carpinum tschonoskii, oak forest with Quercus variabilis and Quercus mongolica and pine forest with Pinus densiflora as the dominant species were separated in mosaic chart by the two dimensional analyses of elevation and soil moisture gradient.

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A new species and a new record of Meghimatium Slugs (Pulmonata: Philomycidae) in Korea

  • Park, Gab-Man
    • Korean Journal of Environmental Biology
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    • v.39 no.3
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    • pp.399-405
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    • 2021
  • Until now, five species (Meghimatium bilineatum, M. fruhstorferi, Limax flavus, L. marginatus, Deroceras reticulatum) in three genera of families Philomycidae and Limacidae have been reported in Korea. Philomycidae is a family of air-breathing land snails. Meghimatium hongdoensis sp. nov. is described based on its middle size (60-80 mm in body length), its body coloration (dark-red yellow), no dorsal with streaks, its genitalia, and 16rDNA sequence analysis. Specimens of this new species were collected from Hongdo island, Sinan-gun, Jeollanam-do, Korea. This species is only known from the type locality at Hongdo to date. It could be found in high-humidity sites. Meghimatium uniforme (Laidlaw 1937) was the first one reported in Korea. It was also collected from Gageodo island, Sinan-gun, Jeollanam-do, Korea. In this study, morphological characteristics including their radula and genital structures of these two species were described. Preliminary results of mitochondrial 16S rDNA sequencing and phylogenetic analyses indicated that these species belonged to the Korean clade.

Multigene Phylogeny and Morphology of Ophiocordyceps alboperitheciata sp. nov., A New Entomopathogenic Fungus Attacking Lepidopteran Larva from Yunnan, China

  • Fan, Qi;Wang, Yuan-Bing;Zhang, Guo-Dong;Tang, De-Xiang;Yu, Hong
    • Mycobiology
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    • v.49 no.2
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    • pp.133-141
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    • 2021
  • A new fungus, Ophiocordyceps alboperitheciata, parasitic on the larva of Noctuidae (Lepidoptera) was identified from a survey of entomopathogenic fungi in Kunming Wild Duck Forest Park, Yunnan Province, China. It can be primarily distinguished from relatives by its longer fertile parts, sterile tips, superficial perithecia, narrower asci, and smaller septa of ascospores. As revealed from phylogenetic analyses inferred from nrSSU, nrLSU, tef-1α, rpb1, and rpb2 sequence data, O. alboperitheciata belongs to the Hirsutella citriformis clade in the genus Ophiocordyceps of Ophiocordycipitaceae, and forms a separated clade from other related species. The uniqueness of the taxon is significantly evidenced by both molecular phylogeny and morphology. Furthermore, the interspecific relationships in the H. citriformis clade are discussed.

Revisiting Rhytisma lonicericola: Morphological Characterization and Molecular Phylogenetic Analysis

  • Jung, Bok-Nam;Park, Ji-Hyun;Shin, Hyeon-Dong
    • Mycobiology
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    • v.50 no.2
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    • pp.150-154
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    • 2022
  • Rhytisma lonicericola was identified as a tar spot fungus on Lonicera sp. in 1902, and has since been recorded on several species of Lonicera in China, Japan, and Korea. Most of the previous records of R. lonicericola have been based on a list of disease occurrences in the absence of any formal morphological identification or molecular analyses. Using six newly obtained specimens collected in the past 2 years, we confirmed the tar spot fungus found on L. japonica in Korea as R. lonicericola based on morphological examinations and molecular phylogenetic analyses. This fungus was distinguished from R. xylostei, another tar spot fungus on Lonicera, by ascospore size and geographical distributions. We present detailed mycological information and, for the first time, DNA sequence data useful for the identification of R. lonicericola.

Pseudomonas sp. strain DJ77에 존재하는 Glutathione S-Transferase 아미노 말단잔기의 Site-directed Mutagenesis

  • Woo, Hee-Jong;Park, Yong-Chjun;Kim, Sungje;Chung, Yong-Je;Chung, An-Sik;Kim, Young-Chang
    • Microbiology and Biotechnology Letters
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    • v.25 no.4
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    • pp.374-378
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    • 1997
  • Glutathione S-transferase (GST) was purified from Pseudomonas sp. DJ77, and its N-terminal sequence was determined to be MKLFISPGACSL. A specific tyrosyl residue in the vicinity of the N terminus is conserved in all the known cytosolic GSTs and has been shown to function as a catalytic residue in $\alpha$, $\mu$, $\pi$ class GSTs from mammals. However, Pseudomonas sp. DJ77 GST has the Phe-4 and Ile-5 instead of Tyr in N-terminus. Its replacement with tyrosine did not significantly affect the enzyme activity. Results from in vitro biochemical analyses were confirmed by the in vivo activity-based CDNB growth inhibition analyses. Our results clearly indicate that GST of Pseudomonas sp. DJ77 has a novel reaction mechanism different from that of mammalian GSTs.

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New Finding of Golovinomyces salviae Powdery Mildew on Glechoma longituba (Lamiaceae), Besides Its Original Host Salvia spp.

  • In-Young Choi;Lamiya Abasova;Joon-Ho Choi;Young-Joon Choi;Hyeon-Dong Shin
    • The Korean Journal of Mycology
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    • v.51 no.3
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    • pp.239-243
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    • 2023
  • The Golovinomyces biocellatus complex is known to consist of powdery mildew from the Golovinomyces genus, associated with host plants from the Lamiaceae family. Recent molecular phylogenetic analyses have resolved the taxonomic composition of this complex, and Golovinomyces biocellatus sensu stricto is considered to be a pathogen of Glechoma species, globally. However, this paper presents a new finding of Golovinomyces salviae on Glechoma longituba, besides its original host species of Salvia. This information was inferred by molecular phylogenetic analyses from the multi-locus nucleotide sequence dataset of intergeneric spacer (IGS), internal transcribed spacer (ITS), large subunit (LSU) of rDNA, and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene. Further, the asexual morphology of this fungus is described and illustrated.