• 한국미생물생명공학회:학술대회논문집
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• 한국미생물생명공학회 1986년도 추계학술대회
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• pp.523.2-523
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• 1986

The hydrolysis of olive oil was attempted with immobilized C. rugosa lipase in the reverse phase solvent system. (i.e. immobilized wet particles is dispersed in continuous phase olive oil or organic solvents containing olive oil). Sephadex LH-20 and LH-60 were used as the supports that can be used in organic solvents. The water content of wet particles of sephadex LH-20 and LH-60 were about 72% (w/w) and 85% (w/w), respectively Both swollen gels with 0.05M buffers adsorbed about 18% of lipase dissolved. They were easily dispersed in liquid olive oil or in organic solvents. The effects of organic solvents on the stability and catalytic activity of the lipase have been examined. The results revealed that isooctane is superior to the other solvents examined for enzymatic fat spliting in reverse phase system. Kinetics of enzymatic hydrolys of olive oil by immobilized lipase has been investigated in a batch reactor. Effects of pH and temperature on the lipase were studied. The substrate concentration was influenced positively on the thermal stability.

• Applied Biological Chemistry
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• 제50권3호
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• pp.204-209
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• 2007

산사 추출물로부터 ${\alpha}-amylase$, ${\alpha}-glucosidase$ 저해활성을 탐색하고 Sephadex LH-20($5{\times}45$ cm)와 MCI-gel CHP-20를 이용하여 저해활성 물질을 정제하고 구조분석을 하였다. 산사 추출물의 페놀화합물의 함량은 물 추출물에서 22.5 mg/g, 60% ethanol 추출물에서 19.2 mg/g으로 나타났으며, 산사 물 추출물 (200 ${\mu}g/ml$)에서 ${\alpha}-amylase$, ${\alpha}-glucosidase$ 저해활성이 각각 100%와 82.6%로 나타났으나, 60% ethanol 추출물(200 ${\mu}g/ml$)에서는 ${\alpha}-glucosidase$ 저해활성이 12%로 낮은 저해활성을 나타내어 산사 물 추출물을 Sephadex LH-20를 이용하여 분획한 결과 I, II, III, IV의 4가지 분획물을 획득하고 다시 Sephadex LH-20과 MCI-gel을 이용하여 nomal phase type인 $EtOH{\rightarrow}H_2O$와 reverse phase type인 $H_2O{\rightarrow}MeOH$로 유기용매의 농도를 증가 시키며 용출하여 A, B, C, D의 compounds를 얻었다. 4가지 compound 중 compound C(200 ${\mu}g/ml$)가 ${\alpha}$-amylase, ${\alpha}$-glucosidase 저해활성이 각각 97.6%, 97.7%로 가장 높게 나타났으며, FAB-MS, NMR과 IR spectrum을 분석한 결과 chlorogenic acid로 동정되었다.

• Journal of the Korean Wood Science and Technology
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• 제29권4호
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• pp.53-59
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• 2001

비자나무 잎을 아세톤-물의 혼합용액으로 추출하고 n-hexane, chloroform, ethylacetate 및 물 분획으로 분류하여 동결건조하고 분말상 화합물로 조제하였다. 그 중에서 ethylacetate 용성 추출물을 메탄올-물 및 에탄올-헥산(n-hexane)의 혼합용액을 사용하여 Sephadex LH-20 칼럼상에서 크로마토그래피를 수행하였다. 비자나무 잎의 추출성분은 대부분 (+)-catechin, (-)-epicatechin, (+)-gallocatechin 및 (-)-epigallocatechin과 같은 flavan 화합물과 3'-O-methyl-(+)-catechin 및 3'-O-methyl-(-)-epicatechin과 같은 flavan의 methyl ether 화합물로 구성되어 있었으며 그 이외에 benzoic acid계 화합물인 protocatechuic acid도 단리 되었다. 단리된 화합물은 $^1H$- 및 $^{13}C$-NMR, NOE와 CI-MS 분석에 의해 화학구조를 구명하였다.

• 대한화학회지
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• 제31권5호
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• pp.457-463
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• 1987

2-브로모프로피오닐화된 수지를 이용해 insulin A (1-21) 사슬을 합성하였다. 시스테인의 결사슬은 각각 acetamidomethyl, benzyl, 그리고 benzhydryl기로 보호하였으며 그루타민과 아스파라긴은 p-nitrophenyl기로 활성화하여 합성에 이용하였다. 매 짝지음단계마다 DCC/HOBT coupling agent로 각 아미노산을 축합하였으며 반응의 완결여부는 닌히드린시험으로 측정하였다. 생성물은 NH$_3$/MeOH-Dioxane(v/v 1:1)로 수지로부터 분리하여 DEAE Sephadex A-25와 Sephadex LH-20으로 정제하였으며 최종생산물은 HPLC, electrophoresis로 확인한 결과 순수한 것(>99.9%)으로 나타났으며 총수득율은 6%이었다.

• Journal of the Korean Wood Science and Technology
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• 제35권6호
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• pp.145-151
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• 2007

산겨릅나무 수피의 성분 연구를 위해 수피를 채취하여 70% 아세톤 용액으로 추출하고 농축한 후 분획깔때기로 헥산, 디클로로메탄, 에틸아세테이트 및 수용성으로 순차 추출하여 동결건조하였다. 에틸아세테이트용성 분획에 대하여 Sephadex LH-20 칼럼크로마토그래피를 반복적으로 수행하여 5개의 페놀성 화합물을 분리하였다. 화합물의 구조는 $^1H-NMR$, $^{13}C-NMR$, 2D-NMR 및 MS 스펙트럼을 분석하여, (+)-catechin (1), (-)-epicatechin (2), (-)-epicatechin-3-O-gallate (3), gallic acid (4) 및 6''-O-galloylsalidroside (5)으로 동정하였다. (-)-epicatechin-3-O-gallate (3), gallic acid (4), 6''-O-galloylsalidroside (5)는 산겨릅나무 수피에서 처음 분리되었다.

• 한국식품저장유통학회지
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• 제8권2호
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• pp.224-230
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• 2001

To develop a material for functional food, the fraction Ⅰ,II and Ⅲ of onion extract were separated by Sephadex LH-20 column. The revealed purification levels of each fraction were 5.2%-6.5%, 12.2%-14.5% and 82.6%-86.7%, respectively. A functional activity test was performed with fraction Ⅲ. The ACE inhibition activity was about 50% at 5mg and about 80% at 10mg. The XOase inhibition activity of Korean Changnyong Daego was 84%, which was the highest. At the experiment of tyrosinase inhibition was detected 21-36% at 10mg. The cholesterol dose in mice blood was as low as low as 79$\pm$5(mg/100 mL) after the intake of fraction Ⅲ. Cholestrol dose in liver was decreased to below 50%. The results show that Sephadex LH-20 column method was very useful as a fraction method for the development of functional food material nsing onions.

• Journal of Microbiology and Biotechnology
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• 제10권2호
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• pp.233-237
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• 2000

As part of a continuing investigation to identify free radical scavengers from the fruit bodies of basidiomycetes, we isolated two p-terphenyl compounds, designated as PAl and PA2l, from cethanolic extract of the fruit body of Pasillus panuoides. The methanolic extract was processed by ethyl acetate extraction and silica gel column chromatography to yield two active fraction. PAl was obtained from one of the fractions through Sephadex LH-20 and silica gel column chromatographies and reverse-phase HPLC. The other fraction was purified by Sephadex LH-20 and reverse-phase column chromatographies to produce PA2. The compounds PA1 and PA2 were identified as leucomentin-4 and leucomentin-2, respectively, on the basis of various spectroscopic analyses. These compounds exhibited strong inhibitory activities against lipid peroxidation in rat liver microsomes with IC_{50}$ values of 0.10 and $0.06{\;}\mu\textrm{g}ml$, respectively.

• Food Science and Biotechnology
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• 제14권2호
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• pp.268-274
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• 2005

Novel antimicrobial substance was isolated from seed coat of Canavalia gladiata by extraction with 75% methanol. Isolation and purification were conducted with solvent fractionation and chromatography on silica gel and sephadex LH-20 columns. Each fraction of antimicrobial activity was tested by paper disc method. Single compound was obtained from the 4th fraction of sephadex LH-20 column chromatography using chloroform/methanol (1:4, v/v), and identified as 3,4,5-trihydroxybenzoic acid methyl ester (methyl gallate) based on HPLC, GC/MS, FT-IR, $^1H$ NMR, and $^{13}C$ NMR analyses. This is the first report describing the presence of methyl gallate in C. gladiata.

• 한국식품영양과학회지
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• 제29권3호
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• pp.395-400
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• 2000

양파 조미액으로부터 ACE 저해활성 물질을 분리하기 위해 hexane, ethylether, ethylacetate, butanol과 물로 분획시, 4.8g의 당 함량과 31.9 mg의 phenol성 물질을 함유한 butanol 분획이 82.1%의 ACE 저해활성을 나타내었고, 70.3%의 ACE 저해활성을 보인 양퍄 조미액보다 높은 저해활성을 보였다. Butanol 분획을 Amberite XAD-2column으로 분리한 결과, ACE 저해활성을 보이는 미흡착 분획(F1)를 얻었다. 활성분획 F1을 Sephadex LH-20column으로 분획한 결과, 4개(F1-1,F1-2,F1-3,F1-4)의 분획을 얻었으며, 이중 F1-3 분획의 ACE 저해활성은 93%로 가장 높은 저해활성을 보였다. Sephadex LH-20 column chromatography에 의해 얻어진 활성분획F1-3을 Supercosil LC-18 column을 이용하여 분리한 결과, 6분대에서 ACE 저해활성을 가지는 단일 peak(F1-3a)를 얻었다. 각 정제 과정에서 얻은 분획들은 전형적인 flavonoid의 band I과 bandII의 피크를 보였다. 또한 ACE에 대한 저해기작은 flavonoid 물질이 보이는 전형적인 비경쟁적 저해양상을 보였다.

• Journal of the Korean Wood Science and Technology
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• 제37권5호
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• pp.480-485
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• 2009

Juglans mandshurica bark was collected and extracted with 70% aqueous acetone. The extracts were concentrated and then sequentially fractionated using n-hexane, $CH_2Cl_2$, EtOAc, and $H_2O$ to be freeze dried. A portion of $H_2O$ fraction was chromatographed on a Sephadex LH-20 column using aqueous methanol to isolate (S)-2,3-HHDP-D-glucopyranoside (1). Some of EtOAc fraction was also treated on a Sephadex LH-20 column using aqueous methanol and EtOH-hexane mixture to purify pedunculagin (2). The structures of these two epimeric ellagitannins were elucidated by spectroscopic tools, NMR and MS.