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Isolation and Identification of Antimicrobial Substance from Canavalia gladiata  

Lee, Hang-Young (RNL life Science Ltd)
Jeong, Heon-Sang (Department of Food Science and Technology, Chungbuk National University)
Publication Information
Food Science and Biotechnology / v.14, no.2, 2005 , pp. 268-274 More about this Journal
Abstract
Novel antimicrobial substance was isolated from seed coat of Canavalia gladiata by extraction with 75% methanol. Isolation and purification were conducted with solvent fractionation and chromatography on silica gel and sephadex LH-20 columns. Each fraction of antimicrobial activity was tested by paper disc method. Single compound was obtained from the 4th fraction of sephadex LH-20 column chromatography using chloroform/methanol (1:4, v/v), and identified as 3,4,5-trihydroxybenzoic acid methyl ester (methyl gallate) based on HPLC, GC/MS, FT-IR, $^1H$ NMR, and $^{13}C$ NMR analyses. This is the first report describing the presence of methyl gallate in C. gladiata.
Keywords
Canavalia gladiata; antimicorbial; 3,4,5-trihydroxybenzoic acid methyl ester; methyl gallate;
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Times Cited By Web Of Science : 4  (Related Records In Web of Science)
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  • Reference
1 Handbook of legumes of world economic importance /
[ James, A.D. ] /
2 Antimicrobial constituents of Rhus glabra /
[ Saxena, G.;McCutcheon, A.R.;Farmer, S.;Towers, G.H.N.;Hancook, R.E.W. ] / J. Etnophamacol.   ScienceOn
3 Inhibition of PhIP mutagenicity by catechins and by the aflavins and gallate Ester /
[ Apostolides, Z.;Balentine, D.A.;Harbowy, M.E.;Hara, Y.;Weisburger, J.H. ] / Mutation Res.
4 Isolation of methyl gallate as the antitumor principle of Acer saccharium /
[ Bailey, A.E.;Asplund, R.O. ] / J. Nat. Prod.
5 Methyl gallate and related polyphenols as auxin protectors /
[ Mendez, J.;Mato, M.C. ] / Phytochem.
6 Thin-layer chromatography of gallic acid, methyl gallate, pyrogallol, phloroglucinol, catechol, resorcinol, hydroquinone, catechin, epicatechin, cinnamic acid, p-coumaric acid, ferulic acid, and tannic acid /
[ Sharm, O.P.;Bhat, T.K.;Singh, B. ] / J. Chromatogr.
7 Purification and characterization of Canavalia gladiata agglutinin /
[ Kojima, K.;Ogawa, H.;Seno, N.;Matsumoto, I. ] / Carbohydrate Research
8 Antibiotic susceptibility testing by standardized single disk method /
[ Bauer, A.W.;Kirby, W.M.M.;Sherris, J.C.;Turck, M. ] / Am. J. Clin. Pathol.
9 Techiniques used for the determination of antimicrobial resistance and sensitivity in bacteria /
[ Piddock, L.J.V. ] / J. Appl. Bacteriol.
10 Inhibitors of tyrosinase and melanogenesis from Galla rhois /
[ Kim, H.J.;Jang, D.I.;Choi, S.W. ] / J. Food Sci. Nutr.
11 Antitumor evaluation of methyl gallate isolated from Acer Ginnala Max /
[ Han, D.S.;Han, S.S.;Lee, C.O.;Rim, Y.S. ] / J. Toxicol. Pub. Health
12 Screening of antioxidants from Indonesian medicinal plants /
[ Sun, W.S.;Roh, J.S.;Oh, S.U.;Lee, J.I.;Oh, W.T.;Kim, J.H. ] / Food Sci. Biotechnol.
13 Isolation and characteristics of new phenolic glycosides of chestnut galls /
[ Ozawa, T.;Haga, K.;Kobayashi, D.;Kamiyama, T.;Takino, Y. ] / Agri. BioI. Chem.
14 Literature review of an underutilized legume: Canavalia gladiata L. /
[ Ekanayake, S.;Jansz, E.R.;Nair, B.M. ] / Plant Foods Human Nutri.
15 Canvanine in Canavalia paraguayensis, C. gladiata. and Dioclea paraguayensis /
[ Schluter, M.;Bordas, E. ] / Phytochem.