• Title/Summary/Keyword: Separation control

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Development and validation of a HPLC method for the simultaneous determination of chlorquinaldol and promestriene in complex prescription (복방제제 내 클로르퀴날돌과 프로메스트리엔에 대한 HPLC 기반 동시분석법의 개발 및 밸리데이션)

  • Lee, Seul-Ji;Shin, Sang-Yeon;Shin, Hae-Jin;Lee, Jin-Gyun;Kim, Dong-Hwan;Lee, Su-Jung;Han, Sang-Beom;Park, Jeong-Hill;Lee, Jeong-Mi;Kwon, Sung-Won
    • Analytical Science and Technology
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    • v.25 no.2
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    • pp.152-157
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    • 2012
  • Currently, many types of compound preparations are being used but the quality control guidelines for their use are lacking. In case of single compound drug, the quality control methods are specified in the pharmacopeia. However, there is no method to simultaneously analyze compound preparations. In this study, a simple validated analytical method for HPLC separation of chlorquinaldol and promestriene is introduced. Validation was divided into categories including linearity, precision, accuracy (recovery) and system suitability. The contents of the products which are on the market were monitored using the validated analytical method and the robustness of the analytical method was tested by conducting an inter-laboratory validation.

Improvement of Immune Activities of Rhodiola sachalinensis A. Bor. by Serial Solvent Fractionization (홍경천의 면역활성이 증진된 용매별 분획 추출물)

  • Ha, Ji-Hye;Kim, Cheol-Hee;Jeong, Hyang-Suk;Jin, Ling;Oh, Sung-Ho;Kim, Seung-Seop;Jeong, Myoung-Hoon;Ma, Choong-Je;Nam, Jong-Hyun;Hwang, Baik;Choi, Geun-Pyo;Park, Uk-Yeon;Lee, Hyeon-Yong
    • Korean Journal of Medicinal Crop Science
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    • v.17 no.3
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    • pp.210-216
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    • 2009
  • This study was performed to compare the effects of immuno-modulating activities of Rhodiola sachalinensis A. Bor. fractionized by consecutive solvent separation. The Cytotoxicity of all fractionized extracts on human kidney cell (HEK293) was lower than crude extracts. Generally, the butanol and chloroform extracts showed less cytotoxicity on about 10.07% and 9.67% than the crude extracts. For human immune B and T cell growth, chloroform fraction showed the highest cell growth compared to the control. The secretion of cytokines (IL-6, $TNF-\alpha$) on human B and T cells were increased by adding chloroform extracts. Also, NK cell growth was significantly improved up to nearly 30% by adding the supernatant of B cell medium grown with the chloroform fraction. It was also found that chloroform fraction could yield higher nitric oxide production from macrophage than untreated control cells. Differentiation of HL-60 cells was increased up to 131.9% after treatment with chloroform fraction extracts, compared to the control. These results indicate that the chloroform fraction of R. sachalinensis have high immune activation activity than others fractions and the crude extracts, implying that this chloroform fractions could be used a new functional material.

The Effect of Genetically Modified Lactobacillus plantarum Carrying Bone Morphogenetic Protein 2 Gene on an Ovariectomized Rat

  • Jin, Eun-Sun;Kim, Ji Yeon;Yang, Jung-Mo;Kim, Jun-Sub;Min, JoongKee;Jeon, Sang Ryong;Choi, Kyoung Hyo;Moon, Gi-Seong;Jeong, Je Hoon
    • Journal of Korean Neurosurgical Society
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    • v.65 no.2
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    • pp.204-214
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    • 2022
  • Objective : Osteoporosis result from age-related decline in the number of osteoblast progenitors in the bone marrow. Probiotics have beneficial effects on the host, when administered in appropriate amounts. This study investigated the effects of probiotics expressing specific genes, especially the effects of genetically modified bone morphogenetic protein (BMP)-2-expressing Lactobacillus plantarum CJNU 3003 (LP) on ovariectomized rats. Methods : Twenty-eight female Wistar rats (250-300 g, 12 weeks old) were divided into four groups : the sham (control), the ovariectomy (OVX)-induced osteoporosis group (OVX), the OVX and LP (OVX/LP), OVX and genetically modified BMP-2-expressing LP (OVX/LP with BMP) groups. The three groups underwent bilateral OVX and two of these groups were administered two different types of LP via oral gavage daily. At 16 weeks post-OVX, blood was collected from the heart and the bilateral tibiae were extracted and were scanned by ex-vivo micro-computed tomography and stained with hematoxylin-and-eosin (H&E) and Masson's trichrome stain for pathological assessment. The serum levels of osteocalcin (OC), rat C-telopeptide of type I collagen (CTX-I), BMP-2, and receptor activator of nuclear factor-ĸB ligand (RANKL) were measured. Results : The 3D-micro-computed tomography images showed that the trabecular structure in the OVX/LP with BMP group was maintained compared with OVX and OVX/LP groups. No significant differences were detected in trabecular thickness (Tb.Th) between control and OVX/LP with BMP groups (p>0.05). Furthermore, a tendency toward increased BMD, trabecular bone volume, Tb.Th, and trabecular number and decreased trabecular separation was found in rats in the OVX/LP with BMP groups when compared with the OVX and OVX/LP groups (p>0.05). The H&E and Masson's trichrome stained sections showed a thicker trabecular bone in the OVX/LP with BMP group compared with the OVX and OVX/LP groups. There was no difference in serum levels of OC, CTX and RANKL control and OVX/LP with BMP groups (p>0.05). In contrast, significant differences were found in OC and CTX-1 levels between the OVX and OVX/LP with BMP groups (p<0.05). Conclusion : Our results showed that the expression of genetically modified BMP-2 showed inhibition effect for bone loss in a rat model of osteoporosis.

A Study on the Spatial Control Effect of Panjang in Donggwoldo (동궐도(東闕圖) 판장(板墻)의 공간통제 효과에 관한 연구)

  • HA Yujeong;KIM Choongsik
    • Korean Journal of Heritage: History & Science
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    • v.55 no.4
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    • pp.196-209
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    • 2022
  • This study compared and analyzed the spatial division function and role of partitions by comparing the entire space and the spatial changes before and after the installation of partitions in <Donggwoldo>, which was manufactured in the late Joseon Dynasty. As a research method, a set standard was prepared to decompose the space of <Donggwoldo> into a unit space, and the standard was set according to the role and height of the space by classifying it into a main space, sub space, and transition space. Two convex maps were prepared according to before and after the installation of the Panjang, and the values of connectivity, control, and integration, which are spatial syntax variables, were calculated and analyzed. The results of the study are as follows. First, the partition in <Donggwoldo(東闕圖)> did not affect the overall spatial arrangement and control or connection of Donggwol, but the movement and access of space is limited to specific areas. Second, the partition was a facility intensively distributed in Naejeon(內殿) and Donggung(東宮) to be used actively in the way of space utilization. It shows that the unit space increased rapidly due to the installation of the partition. Since the partition was installed in the spaces that were open and under high control in the case of Naejeon(內殿), it helped to secure private spaces as closed ones under low control. On the other hand, for Donggung(東宮), the spaces were compartmented and divided with the partition to guide the movement path through narrow gates of the partition and increase the depth of the space. This helped to create spaces that are free and can be hidden as it increased the number of spaces coming through. Third, In addition to the functions of "eye blocking, space division, and movement path control" revealed in prior research, the partition has created a "space that is easy to control" within a specific area. The installation of the partition reduced the scale through the separation of spaces, but it occurred the expansion of the movement path and space. Also, the partition functioned to strengthen hiding and closure or increase openness as well through space division. This study is significant in that it revealed the value of the spatial control function of panjang through the analysis of spatial control and depth by analyzing the function of the partition with a mathematical model in addition to the analysis and study of the function and role of panjang. In addition, it is valuable in that it has prepared a framework for analysis tools that can be applied to traditional residential complexes similar to palaces by applying space syntax to <Donggungdo> to create convex spaces according to unit space division and connection types of palace architecture and landscape elements.

Laboratory Investigation into Factors Affecting Performance of Anaerobic Contact Process for Pear Processing Wastewater

  • Hur Joon Moo;Son Bu Soon;Jang Bong Ki;Park Jong An;Lee Jong Whoa;Kim Joon Hyun
    • Environmental Sciences Bulletin of The Korean Environmental Sciences Society
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    • v.2 no.2
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    • pp.99-108
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    • 1998
  • Results obtained from this research showed that the anaerobic contact process was applicable to pear waste with COD removal efficiencies of up to $95\%$ depending on conditions, provided ammonium and phosphate salts were added as well as other nutrients, present in the commercial fertilizer, Milorganite or in yeast extract. These latter materials were required in minimum concentrations of 5 and 1.5 g/L, respectively, in the feed independent of HRT and volatile solids loading rate, with part of the effect due to the mineral fraction. Digestion was satisfactory over the whole range of volatile solids loading rates and liquid retention time of 30 to 0.5 days tested, although treatment efficiency dropped off noticeably between 1 and 0.5 day liquid retention time because of poorer flocculation and separation of anaerobic bacteria. Settling of anaerobic bacteria including methane producing bacteria was related to settling of mixed liquor suspended solids only at 1 to 5 days liquid retention times, at other liquid retention times anaerobic microorganism settled markedly less efficiently than mixed liquor suspended solids. Further studies are being made to provide information of practical and basic interest. Data on the composition of the active fraction of yeast extract might solve many practical nutrient problems encountered with the anaerobic contact process and improve its economics. Further improvement in the flocculation and settling of anaerobic bacteria as well as other bacteria would improve overall performance and allow the use of shorter liquid retention times with dilute waste. Knowledge about the numbers of methane formers present would allow a degree of understanding and control of the process not presently attainable.

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A STUDY ON THE FLOW CHARACTERISTICS OF AIR-KNIFE USING A CONSTANT EXPANSION RATE NOZZLE (팽창률이 일정한 노즐을 사용한 AIR-KNIFE 유동에 관한 연구)

  • Lee, Dong-Won;Kang, Nam-Cheol;Kim, Guen-Young;Kwon, Young-Doo;Kwon, Soon-Bum
    • Journal of computational fluids engineering
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    • v.13 no.4
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    • pp.1-7
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    • 2008
  • In the process of continuous hot-dip galvanizing, it is well known that the gas wiping through an air knife system is most effective because of its uniformity in coating thickness, possibility of thin coating, workability in high speed, and simplicity of control. However, gas wiping used in the galvanizing process brings about a problem of splashing at the strip edge above a certain high speed of process. It is also known that the problem of edge splashing is more harmful than that at the mid strip surface. For a given liquid(of a certain viscosity and surface tension), the onset of splashing mainly depends upon the strip velocity, the gas-jet pressure, and the nozzle's stand-off distance. In these connections in the present study, we proposed three kinds of air knife system having nozzles of constant expansion rate, and compared the jet structures issuing from newly proposed nozzle systems with the result by a conventional one. In numerical analysis, the governing equations are consisted of two-dimensional time dependent Navier-Stokes equations, and the standard k-${\varepsilon}$ turbulence model is employed to solve turbulence stress and so on. As the result, it is found that we had better use the constant expansion-rate nozzle which can be interpreted from the point view of the energy saving for the same coating thickness. Also, we better reduce the size of separation bubble and enhance the cutting ability at the strip surface, by using an air-knife having constant expansion-rate nozzle.

A study on the color controlled of painter's work (페인트 도장공사의 색관리에 관한 연구)

  • Shim, Myung-Sup;Lee, Hyun-Jeong
    • Journal of the Korea Institute of Building Construction
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    • v.3 no.1
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    • pp.107-114
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    • 2003
  • This study aims to find methods that prevents aging of buildings paint coating and that limits defects in construction. Defects in painting can occur in four stages: pure paint, during painting, after the paint coating has dried, and after some period of time has passed after coating. Paint may become bad due to precipitation of pigments, formation of membranes, and seeding during manufacturing. Therefore, it is important that the paint is well mixed and kept airtight at a cool, dark place. Indents, paint brush strokes, orange peel, separation of colors, and paint running and spreading during the paint work process can be prevented by using high quality materials and applying a high-level of construction method. After the paint coating has dried, boiling, yellowing, poor drying, poor bonding, and/or glen deficiency may occur. These are influenced by the levels of cleanness of the dried product, drying temperature and hydration. Then, when the coating has been left dried for some period of time, cracking, peeling, scaling, swelling, discoloring, and/or rusting may develop due to the ultraviolet and contaminants in the air. Since these defects occur due to inappropriate construction schedule and/or hot and humid condition, one must use weatherproof materials. Furthermore, poor paint color may be caused by contamination in the sample plate, discoloration, and/or discrepancies in colors which are due to material differences, level of glossiness, degree of dispersion, dual color property of metallic colors, precipitation of pigments, etc. One should achieve reduction in construction cost and effectiveness in paint work by limiting contaminations in the construction site and strictly observing to construction regulations.

Separation and Identification of Antimicrobial Substances from Green Tea Extracts (녹차추출물로부터 항균물질의 분리 및 구조동정)

  • Shin, Young-Hee;Lee, Seung-Cheol;Choi, Sung-Gil;Heo, Ho-Jin;Cho, Sung-Hwan
    • Food Science and Preservation
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    • v.16 no.6
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    • pp.924-928
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    • 2009
  • Green tea has been shown to have multifunctional health-promoting properties including cholesterol level control an antidiabetic effect and anticancer, antioxidant, and antimicrobial properties, inboth in vivo and in vitro experiments.We earlier reported antifungal and antibacterial effects of green tea extract by investigating cell membrane functions. The purpose of the present study was to purify and identify antimicrobial substances from green tea extract. Such materials were extracted from green tea (Camellia sinensis. var. sinensis) and purified by high-performance liquid chromatography. The antimicrobial substances in the extract were identified as epicatechin gallate and epigallocatechin gallate by nuclear magnetic resonance spectrophotometry.

$Fasciola$ $gigantica$ Fatty Acid Binding Protein (FABP) as a Prophylactic Agent against $Schistosoma$ $mansoni$ Infection in CD1 Mice

  • Aly, Ibrahim Rabia;Diab, M.;El-Amir, A.M.;Hendawy, M.;Kadry, S.
    • Parasites, Hosts and Diseases
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    • v.50 no.1
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    • pp.37-43
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    • 2012
  • Although schistosomicidal drugs and other control measures exist, the advent of an efficacious vaccine remains the most potentially powerful means for controlling this disease. In this study, native fatty acid binding protein (FABP) from $Fasciola$ $gigantica$ was purified from the adult worm's crude extract by saturation with ammonium sulphate followed by separation on DEAE-Sephadex A-50 anion exchange chromatography and gel filtration using Sephacryl HR-100, respectively. CD1 mice were immunized with the purified, native $F.$ $gigantica$ FABP in Freund's adjuvant and challenged subcutaneously with 120 $Schistosoma$ $mansoni$ cercariae. Immunization of CD1 mice with $F.$ $gigantica$ FABP has induced heterologous protection against $S.$ $mansoni$, evidenced by the significant reduction in mean worm burden (72.3%), liver and intestinal egg counts (81.3% and 80.8%, respectively), and hepatic granuloma counts (42%). Also, it elicited mixed $IgG_1/IgG_{2b}$ immune responses with predominant $IgG_1$ isotype, suggesting that native $F.$ $gigantica$ FABP is mediated by a mixed Th1/Th2 response. However, it failed to induce any significant differences in the oogram pattern or in the mean granuloma diameter. This indicated that native $F.$ $gigantica$ FABP could be a promising vaccine candidate against $S.$ $mansoni$ infection.

A Study on 'Line Balancing' of Women's Jacket Production (여성복 재킷 생산라인의 라인 밸런싱에 관한 연구 - 공정편성 효율을 중심으로 -)

  • Shim, Kue-Nam;Kim, Jin-Seon;Oh, Ji-Yeong;Suh, Eun-Joung
    • Fashion & Textile Research Journal
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    • v.16 no.6
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    • pp.979-986
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    • 2014
  • This study establishes basic data for operations management by organizing processes and measuring time in the mini line for female jackets to improve productivity, ensure competitiveness, and maintain operator competency and the line process flow balance between apparel manufacturing companies. The results of this study are as follows. Sewing operations are divided into preparation functions, arrangement, partial tasks, and assembly that consist of 84 processes. The results from time measurement indicate that 3238.41seconds (sec) were required to produce a single jacket and that the average time required for operators was 231.32 sec. A control limit was established to increase the reliability of the measured value for net time. After outside values were removed, the operation time was measured to be 3176.35 sec. This accounted for 98.08% of the total operation time, with net time decreasing by 62.06. Skill and effort level coefficients were applied to measure the operator performance, the total real time was calculated to be 3415. The requirement for preparation and arrangement operations were 1233.35 sec, and 2182.22 sec for partial tasks and assembly operations. Process separation and organization were performed after the bottleneck operation was selected to identify the maximum line balance. Consequently, process efficiency of preparation and arrangement operations increased from 79.19% to 93.00%, and the partial tasks and assembly operations increased from 62.36% to 90.93%.