• Title/Summary/Keyword: Separation Behavior Analysis

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Cluster Cell Separation Algorithm for Automated Cell Tracking (자동 세포 추적을 위한 클러스터 세포 분리 알고리즘)

  • Cho, Mi Gyung;Shim, Jaesool
    • Transactions of the Korean Society of Mechanical Engineers B
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    • v.37 no.3
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    • pp.259-266
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    • 2013
  • An automated cell tracking system is used to automatically analyze and track the changes in cell behavior in time-lapse cell images acquired using a microscope with a cell culture. Clustering is the partial overlapping of neighboring cells in the process of cell change. Separating clusters into individual cells is very important for cell tracking. In this study, we proposed an algorithm for separating clusters by using ellipse fitting based on a direct least square method. We extracted the contours of clusters, divided them into line segments, and then produced their fitted ellipses using a direct least square method for each line segment. All of the fitted ellipses could be used to separate their corresponding clusters. In experiments, our algorithm separated clusters with average precisions of 91% for two overlapping cells, 84% for three overlapping cells, and about 73% for four overlapping cells.

Optimization of Radiostrontium Separation Process Using Sr Resin (Sr resin을 이용한 방사성 스트론튬 분리의 최적화)

  • Jung, Yoonhee;Kim, Hyuncheol;Suh, Kyung Suk;Kang, Mun Ja;Chung, Kun Ho
    • Journal of Nuclear Fuel Cycle and Waste Technology(JNFCWT)
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    • v.13 no.2
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    • pp.123-130
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    • 2015
  • For the analysis of 90Sr, which is a pure beta emitter, radiochemical separation from the main interfering elements such as Ca, Ba and Ra is required due to their similarity in chemical behavior to strontium. This study describes a radioanalytical procedure using extraction chromatography for separating Sr from interfering elements. The maximum capacity of the resin for Sr was approximately 6 mg per 1.5 mL of bed volume (BV). The recovery of Sr on the resin 1.5 mL (BV) was quantitative for the calcium level of 200 mg at the flow rate of 1 mL min-1. However the chemical yield declined by increasing the flow rate by up to 5 mL min-1 even at the calcium level of 200 mg. When using the same BV of Sr resin, the performance of the resin was enhanced as the cross-sectional area of the Sr resin column is small.

A Numerical Analysis of the Binary Droplet Collision by Using a Level Set Method (레벨셋 방법을 이용한 액적 충돌에 대한 수치해석)

  • Lee, Sang-Hyuk;Hur, Nahm-Keon
    • Transactions of the Korean Society of Mechanical Engineers B
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    • v.35 no.4
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    • pp.353-360
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    • 2011
  • The prediction of binary droplet collisions is important in the formation of falling drops and the evolution of sprays. The droplet velocity, impact parameter, and drop-size ratio influence the interaction between the droplets. The effect of these parameters results in complicated collision phenomena. Droplet collisions can be classified into four types of interactions: bouncing, coalescence, reflexive separation, and stretching separation. In the present study, the interfacial flow problem of the droplet collision was numerically simulated by using the level set method. 2D axisymmetric simulations on the head-on collisions and 3D simulation on the off-center collisions were performed. The numerical results of droplet behavior after the collision agreed well with the experimental and analytical results. The mixing of the mass of the initial droplets after the collision was also predicted by using different species index of colliding droplets.

Simulation of Preparation Protein Chromatography (제조용 단백질 크로마토그래피의 시뮬레이션)

  • 김인호;이선묵;황우성
    • KSBB Journal
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    • v.14 no.3
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    • pp.371-376
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    • 1999
  • Simulation of preparative protein chromatography becomes necessary for separation as well as optimal operation. A mathematical model describing the behavior of elution peaks in preparative protein chromatography for single and binary component separation was solved numerically using a PDEsolver Macsyma$^{\circledR}$(Macsyma Inc., Arlington, MA, U.S.A.). Band profiles were calculated with the equilibrium-dispersive model of chromatography. The effects of the sample volume, concentrations of solutes in the sample, flow velocity and column length on the band profile of the elution peaks are discussed. The results in this paper suggest the model simulation for the binary mixture can be extended to multicomponent separations.

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Structural, optical, and electrical properties on Cu(In,Ga)$Se_2$ thin-films with Cu-defects and In/(In+Ga) ratio (Cu(In,Ga)$Se_2$ 박막의 Cu 결함 및 In, Ga 비율의 변화에 따른 구조적, 광학적, 전기적 특성 연구)

  • Jeong, A.R.;Kim, G.Y.;Jo, W.;Jo, H.J.;Kim, D.H.;Sung, S.J.;Kang, J.K.;Lee, D.H.;Nam, D.H.;Cheong, H.
    • 한국신재생에너지학회:학술대회논문집
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    • 2011.11a
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    • pp.47.1-47.1
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    • 2011
  • We report on a direct measurement of two-dimensional chemical and electrical distribution on the surface of photovoltaic Cu(In,Ga)$Se_2$ thin-films using a nano-scale spectroscopic and electrical characterization, respectively. The Raman measurement reveals non-uniformed surface phonon vibration which comes from different compositional distribution and defects in the nature of polycrystalline thin-films. On the other hand, potential analysis by scanning Kelvin probe force microscopy shows a higher surface potential or a small work function on grain boundaries of the thin-films than on the grain surfaces. This demonstrates the grain boundary is positively charged and local built-in potential exist on grain boundary, which improve electron-hole separation on grain boundary. Local electrical transport measurements with scanning probe microscopy on the thin-films indicates that as external bias is increases, local current is started to flow from grain boundary and saturated over 0.3 V external bias. This accounts for carrier behavior in the vicinity of grain boundary with regard to defect states. We suggest that electron-hole separation at the grain boundary as well as chemical and electrical distribution of polycrystalline Cu(In,Ga)$Se_2$ thin-films.

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Operational Variables and Performance of Hydrodynamic Separator Treating Rainfall Runoff from Bridge (수리동력학적 분리장치에 의한 교량에서의 비점원 오염물질 처리시 운전변수와 분리효율에 관한 연구)

  • Kim, Yeonseok;Yu, Jianghua;Kim, Youngchul
    • Journal of Korean Society on Water Environment
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    • v.27 no.3
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    • pp.342-348
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    • 2011
  • A hydrodynamic separator using natural free energy provided by bridge was operated for the treatment of stormwater runoff. The separator was automatically controlled by using electronic valve which is connected with pressure meter. Normally the separator was opened during dry days, but it was closed after the capture of first flush. The results indicated that the average pressure and the flow rate were directly affected by the rainfall intensity. The pressure was more than 3 meters as the rainfall intensity was above 5 mm/hr. The percent volume of underflow decreased as the pressure and flow rate increased, but the percent volume of overflow showed an opposite behavior. The concentration of total suspended solids (TSS) in underflow increased as a function of increasing pressure while it decreased in overflow. The TSS separation efficiency was evaluated based on mass balance. It ranged from 30% to 90% with the pressure ranging from 2 to 10 meters, and it was proportional to pressure and flow rate. The analysis of water balance indicated that around 13% of total runoff was captured by the separator as a first flush, and this runoff was separated as underflow and overflow with the respective percent volume of 29% and 71%. The pollutants budget was also examined based on mass balance. The results showed that the percent of TSS, $COD_{cr}$, TN and TP in underflow were 73%, 59%, 7.6% and 49%, respectively.

Three Dimensional Thermal Cycle Analysis of Mold in Repeated Forming Process of TV Glass (TV 유리의 반복 성형공정에서 3차원 금형 열사이클 해석)

  • Hwang, Jung-Hea;Choi, Joo-Ho;Kim, Jun-Bum
    • Proceedings of the KSME Conference
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    • 2000.11b
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    • pp.192-198
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    • 2000
  • Three dimensional thermal cycle analysis of the plunger is carried out in repeated forming process of the TV glass, which is continued work of two dimensional analysis where an efficient method has been proposed. The plunger undergoes temperature fluctuation during a cycle due to the repeated contact and separation from the glass, which attains a cyclic steady state having same temperature history at every cycle. Straightforward analysis of this problem brings about more than 90 cycles to get reasonable solution. An exponential function fitting method is proposed, which finds exponential function to best approximate temperature values of 3 consecutive cycles, and new cycle is restarted with the fitted value at infinite time. Number of cases are analyzed using the proposed method and compared to the result of straightforward repetition, from which one finds that the method always reaches nearly convergent solution within $9{\sim}12$ cycles, but turns around afterwards without further convergence. Two step use is found most efficient, in which the exponential fitting is carried out fer the first 12 cycles, followed by simple repetition, which shows fast convergence expending only 6 additional cycles to get the accuracy within 2 error. This reduces the computation cycle remarkably from 90 to 18, which is 80% reduction. From the parametric studies, one reveals that the overall thermal behavior of the plunger in terms of cooling parameters and time is similar to that of 2 dimensional analysis.

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Facile Synthesis of In2S3 Modified Ag3PO4 Nanocomposites with Improved Photoelectrochemical Properties and Stabilities

  • Zeng, Yi-Kai;Bo, Shenyu;Wang, Jun-hui;Cui, Bin;Gu, Hao;Zhu, Lei;Oh, Won-Chun
    • Korean Journal of Materials Research
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    • v.30 no.11
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    • pp.601-608
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    • 2020
  • In this work, Ag3PO4/In2S3 nanocomposites with low loading of In2S3 (5-15 wt %) are fabricated by two step chemical precipitation approach. The microstructure, composition and improved photoelectrochemical properties of the as-prepared composites are studied by X-ray diffraction pattern (XRD), field emission scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS), photocurrent density, EIS and amperometric i-t curve analysis. It is found that most of In2S3 nanoparticles are deposited on the surfaces of Ag3PO4. The as-prepared Ag3PO4/In2S3 composite (10 wt%) is selected and investigated by SEM and TEM, which exhibits special morphology consisting of lager size substrate (Ag3PO4), particles and some nanosheets (In2S3). The introduction of In2S3 is effective at improving the charge separation and transfer efficiency of Ag3PO4/In2S3, resulting in an enhancement of photoelectric behavior. The origin of the enhanced photoelectrochemical activity of the In2S3-modified Ag3PO4 may be due to the improved charge separation, photocurrent stability and oriented electrons transport pathways in environment and energy applications.

Simultaneous analysis method of 89Sr and 90Sr in liquid sample using automated separation system (자동핵종분리장치를 이용한 액체시료 중 89Sr, 90Sr 동시분석법 연구)

  • Kim, Heewon;Lee, Yong-Jin;Kim, Sun-Ha;Lee, Jin-Hong;Lim, Jong-Myoung;Kim, Hyuncheol
    • Analytical Science and Technology
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    • v.33 no.6
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    • pp.274-284
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    • 2020
  • This study described the analytical method for simultaneous determination of 89Sr and 90Sr in liquid sample using automated separation system. Radiostrontium in 0.5 kg of liquid sample was concentrated as SrCO3 to reduce the volume of sample, and purified from the sample using Sr-resin 2 mL (BV, Bed volume). The behavior of Sr and interferences such as Ba, Ca and Y were estimated with various flow rate ranging from 1 to 4 mL min-1. The detailed procedure for the purification of Sr on Sr-resin was presented. The purified radiostronitum was measured in Cerenkov mode and then measured in Scintillation mode by mixing scintillation cocktail. The measured value in both modes were used to calculate the activity of 89Sr and 90Sr. The performance tests were carried out the lab-control-sample having various activity ratio of between 89Sr and 90Sr. The recovery of Sr was ranged from 68 to 94 %. The relative bias of 89Sr activity was ranged from -5 to 20 %, and it was ranged from -10 to 10 % for 90Sr.

Development of Vanadium Recovery Process Using Reduction Pre-treatment from Vanadium Titanium-Magnetite (VTM) Ore (VTM광으로부터 환원 전처리를 이용한 바나듐 회수 공정 개발)

  • Go, Byunghun;Jeong, Dohyun;Han, Yosep;Kim, Seongmin;Chu, Yeoni;Kim, Byung-su;Jeon, Ho-Seok
    • Resources Recycling
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    • v.31 no.2
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    • pp.12-19
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    • 2022
  • The study was conducted to develop a vanadium recovery process using reduction pre-treatment in the Vanadium TitanoMagnetite (VTM) The sample for the research was provided by the Gwan-in Mine in Pocheon, Gyeonggi-do. The vanadium content of the sample is 0.54 V2O5% and vanadium is concentrated mainly in magnetite and ilmenite. Magnetic separation of the sample can increase vanadium content up to 1.10 V2O5%. To increase the vanadium content further, reduction pre-treatment was performed, which is a process of concentrating vanadium present in the iron by reducing iron in magnetite using carbon(C). Based on this reduction pre-treatment, the magnetic separation process was developed, which achieved a vanadium grade of 1.31V2O5% and 79.68% recovery. In addition, XRD analysis of the vanadium concentrate before and after reduction and the final vanadium concentrate was performed to confirm the behavior of vanadium by reduction pre-treatment.