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Bacterial Reverse Mutation Test Evaluation of Hydrolyzed GMP Powder Containing Highly Concentrated Sialic Acid (23%) produced by Enzyme Separation and Solvent Enrichment Method (효소분리 및 용매정제법으로 제조한 고농도 Sialic Acid(23%)가 함유된 GMP 가수분해분말의 미생물복귀돌연변이시험 연구)

  • Kim, Hee-Kyong;Cho, Hyang-Hyun;Noh, Hye-Ji
    • Journal of Dairy Science and Biotechnology
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    • v.34 no.2
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    • pp.91-98
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    • 2016
  • The goal of this study was to develop hydrolyzed whey protein powder (23%-GNANA) manufactured with high content of sialic acid, a marker compound that is usually present at 7% concentration in GMP obtained from the milk protein. It is a safe food, used worldwide in infant and baby foods, etc. The test substance was prepared using (7% sialic acid containing) GMP as a raw material. Alcalase, an enzyme approved as a food additive, was used after separating sialic acid, with 100% efficiency, and 23%-GNANA (composed of 23% sialic acid and protein; product name: HELICOBACTROL-23), provided by MEDINUTROL Inc. (Korea), manufactured to have high (23%) content through ethanol soaking and enrichment. Bacterial reverse mutation (Ames) test was conducted in accordance with the GLP Guideline using the test substance specified above. To detect its mutagenicity potential in microorganisms, histidine auxotrophic strains of Salmonella typhimurium, TA98, TA100, TA1535, and TA1537, and tryptophan auxotrophic Escherichia coli strain, WP2uvrA, were used. The bacterial reverse mutation (Ames) test was performed using five concentrations of the test substances (0, 61.7, 185, 556, 1,670, $5,000{\mu}g/plate$). The evaluation did not reveal repetitive increase of colony generating values and positive criteria for reverse mutagenicity for any tested concentration in the five strains regardless of the presence of metabolic activation system, and no dose-dependency. In conclusion, the safety of 23%-GNANA test substance was verified by the bacterial reverse mutation test conducted before registration of 23%-GNANA as a food additive.

Multi-User X-Channel Interference Alignment in 5 Generation MIMO Mobile Communications (5세대 MIMO 이동 통신의 다중 사용자 X 채널 간섭 정렬)

  • Kim, Jeong-Su;Lee, Moon Ho
    • The Journal of the Institute of Internet, Broadcasting and Communication
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    • v.17 no.5
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    • pp.61-69
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    • 2017
  • The study on interference channel is very important information theoretically and many studies have been done on it. However, even in the simplest case, even in the case of two user interfering channels, the channel capacity is not yet known except in special cases. Recently, research on the multiplexing gain that shows the tendency of the transmission rate in the high signal to noise ratio (SNR) band has been actively carried out, instead of accurately grasping the channel capacity. Obtaining optimal multiplexing gain can reveal trends in channel capacity at high signal-to-noise ratio bands. In an interfering channel with two users, the best multiplexing gain can be obtained by eliminating the interference. However, recent research shows that when the number of users is more than three, the optimal multiplexing gain can not be obtained only by zero forcing and a new technique called interference sorting is needed. There are two types of interference sorting techniques. Beamforming A method of effectively separating signals and interference by properly selecting matrices and constructing structured codes using rational numbers and irrational numbers. The interference alignment technique can achieve optimal multiplexing gain in various environments such as interference channel, X channel, compound broadcast channel, and multi hop network for multi source multi destination. In recent years, it has also been applied to distributed storage. Lee et al., "Lattice Code Interference Alignment in Cooperative Multipoint Transmission (COMP) for Interference Channels of Three Users", Journal of the Institute of Electronics Engineers, vol.49-TC,no.6,2012. In this paper, the DoF of delayed channel information is obtained.

A Study on the Quality Characteristics of Rice Beer Using Brewing Yeast isolated from Nuruk (누룩에서 분리한 양조용 효모를 이용한 쌀맥주의 품질특성 연구)

  • Lee, Young Bog;Ko, Dong Jun;Cheong, Chul
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.20 no.12
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    • pp.340-347
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    • 2019
  • This study investigated the brewing properties of rice beer made with yeast isolated from traditional whole wheat nuruk. The experimental method was followed by alcohol fermentation and quality analysis for six months after separating the yeast from nuruk. The yeasts isolated from traditional nuruk showed normal fermentation characteristics, like those of commercial yeast, in terms of alcohol production capability, sugar content, and pH reducing power. Especially, the yeast (KCCM 301) isolated from whole wheat nuruk showed higher contents of glycogen and trehalose than that of commercial yeast, and so KCCM 301 yeast has excellent yeast vitality. Meanwhile, the yeast (KCCM 90301) isolated from traditional Nuruk produced significantly higher alcohol and ester contents than that of commercial yeast. This has a positive effect for supplementing the taste and aroma of rice beer. In conclusion, the yeasts isolated from whole wheat nuruk showed the general alcohol fermentation pattern and aroma content of rice beer. These yeasts seem to be effective in strengthening the flavor of rice beer.

An analysis on the development processes of mathematics and the results by dialectical materialism (수학의 발달과정과 그 결과에 대한 변증법적 유물론에 의한 분석)

  • 조윤동
    • Journal of Educational Research in Mathematics
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    • v.13 no.3
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    • pp.329-349
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    • 2003
  • Mathematics education is accomplished by systems such as mathematical curriculum and tools such as a textbook which reflects such systems. Human beings make such systems and tools. Therefore, a viewpoint of mathematics of those who make them is an important factor. The view point of mathematics is formed during doing and learning mathematics, but the already formed viewpoint of mathematics affects doing and teaching mathematics. Hence, it will be a factor which affects basically that those who employ themselves on mathematics education have a certain viewpoint of mathematics. This article presents dialectical materialistic viewpoint as the viewpoint of mathematics which affects fundamentally on mathematical teaching-learning practice. The dialectical materialism is carried through the process and result of mathematics development. This shows that mathematical knowledge is objective. Mathematical knowledge has developed according to three basic rules of dialectical materialism i.e. the transformation of quantity into quality, the unification of antagonistic objects, and the negation of negation. This viewpoint of mathematics should offer the viewpoint of mathematics education which is different from the view point of absolutism, relativism or formal logic. In this article I considered mathematics separating standpoint of mathematics into materialistic viewpoint and dialectical viewpoint. 1 did so for the convenience of analysis, but you will be able to look at the unified viewpoint of dialectical materialism. 1 will make mention of teaching-learning method on another occasion.

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Determination of Rock Cleavages Using AMS (Anisotropy of Magnetic Susceptibility): a Case Study on the Geochang Granite Stone, Korea (대자율이방성(AMS) 분석을 통한 석재 결의 파악: 거창 화강석에서의 사례 연구)

  • Cho, Hyeongseong;Kim, Jong-Sun;Kim, Kun-Ki;Kang, Moo-Hwan;Sohn, Young Kwan;Lee, Youn Soo;Jwa, Yong-Joo;Son, Moon
    • The Journal of the Petrological Society of Korea
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    • v.24 no.3
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    • pp.209-231
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    • 2015
  • In granite quarry, stones are generally quarried along easily separating planes called as 'rock cleavage'. Because orientation and characteristics of the rock cleavage are directly involved with easy quarrying, it is the most important factor on selecting a direction of digging. Using AMS (anisotropy of magnetic susceptibility), we attempt to interpret rock fabrics in Geochang Granite Stone (JS, SD, AR, GD, BW, MD quarry) and discuss about determination of rock cleavages and correlation between the rock fabrics and cleavages. Based on mean susceptibility, thermo-susceptibility curves, and hysteresis parameters, Ti-poor MD and/or PSD magnetites are the main contributor to AMS of the granite stones. The systematic magnetic foliations with sub-vertical dip angle are developed in the whole granite quarries. In most of the granite quarries, the magnetic foliations are significantly consistent with grain plane. In the BW quarry, which has higher $P_J$ values than the others, the magnetic foliations coincide exceptionally with rift plane. These results suggest that rock cleavages in granite stone are related to rock fabrics meaning shape and spatial arrangement of crystals. Magnetic fabrics analysis using AMS method, therefore, can be a quantitative and effective tool for determination of rock cleavages in granite quarry.

Evaluations of Life Cycle Assessment on Indium-Tin-Oxide Electrochemical Recycling Process (디스플레이 투명전극용 인듐-주석-산화물의 전기화학적 재활용 공정에 관한 전과정 평가)

  • Kim, Raymund K.I.;Lee, Na-Ri;Lee, Soo-Sun;Lee, Young-Sang;Hong, Sung-Jei;Son, Young-Keun;Hong, Tae-Whan
    • Clean Technology
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    • v.19 no.4
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    • pp.388-392
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    • 2013
  • Iindium-tin-oxide (ITO) material was had to use in display application as transparent electrode. However it would be problems comes up, the depletion of indium, tin and energy consumption of production process. Therefore recently trend was demanded alternative ITO material and recycling/reused ITO. In this conditions, the environmental impact have to express correct value about recycling/reused ITO process. The life cycle assessment was valuable method in this process. Thus first step was carried out separating in/out put (material) sources and then, exactive data base (DB) was applied. The result of environment impact was calculated by affect categories and recycling rate was set to 34% (This value was measured in previous project). The rate (g) of ITO material was calculated by chemical equivalent. In result, environmental impact were revealed acidification potential and abiotic depletion and if do not recycle/reuse ITO, $ 476 per 1 ton waste in land.

A Study on Rainfall-Runoff Analysis by Geomorphological Instantaneous Unit Hydrograph (GIUH) (지형학적 순간단위도(GIUH)에 의한 강우-유출해석)

  • Choi, Hung-Sik;Park, Chung-Soo;Moon, Hyung-Geun
    • Journal of the Korean Society of Hazard Mitigation
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    • v.6 no.1 s.20
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    • pp.49-58
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    • 2006
  • Rainfall-runoff characteristics are analysed based on the geomorphological instantaneous unit hydrograph(GIUH) derived by geomorphological parameters using geographical information system in watershed ungaged or deficient of field data. Observed data of Seom river experiment watershed at upstream of Hoengseong dam and variable slope method for hydrograph separating of direct non are used. The 4th stream order of Seom river experimental watershed is developed with a regular correlation referred to the Horton-Strahler's law of stream order. The characteristic velocity to determine shape parameter of GIUH is 1.0m/s and its equation is modified for accurate results. Hydrograph at the outlet of 4th stream order of Maeil gage station and at the outlets of 3rd stream order of Sogun and Nonggeori gage stations show a little differences in falling limb of hydrograph but agree well to the observed data in general. The results by hydrological routing with HEC-HMS to the outlet of 4th stream order of Maeil gage station which the hydrograph by GIUH obtained at Sogun and Nonggeori gage stations of 3rd stream oder are applied as upstream inputs give better agreement with observed data than those by hydrograph by GIUH obtained at Maeil gage station of 4th stream order. In general, the rainfall-runoff by GIUH has applicability to the watershed routing of ungaged project regions.

Speech Animation Synthesis based on a Korean Co-articulation Model (한국어 동시조음 모델에 기반한 스피치 애니메이션 생성)

  • Jang, Minjung;Jung, Sunjin;Noh, Junyong
    • Journal of the Korea Computer Graphics Society
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    • v.26 no.3
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    • pp.49-59
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    • 2020
  • In this paper, we propose a speech animation synthesis specialized in Korean through a rule-based co-articulation model. Speech animation has been widely used in the cultural industry, such as movies, animations, and games that require natural and realistic motion. Because the technique for audio driven speech animation has been mainly developed for English, however, the animation results for domestic content are often visually very unnatural. For example, dubbing of a voice actor is played with no mouth motion at all or with an unsynchronized looping of simple mouth shapes at best. Although there are language-independent speech animation models, which are not specialized in Korean, they are yet to ensure the quality to be utilized in a domestic content production. Therefore, we propose a natural speech animation synthesis method that reflects the linguistic characteristics of Korean driven by an input audio and text. Reflecting the features that vowels mostly determine the mouth shape in Korean, a coarticulation model separating lips and the tongue has been defined to solve the previous problem of lip distortion and occasional missing of some phoneme characteristics. Our model also reflects the differences in prosodic features for improved dynamics in speech animation. Through user studies, we verify that the proposed model can synthesize natural speech animation.

A Biological Complex Soil Treatment Process Using Selected Soil Bacterial Strains (현장 미생물을 이용한 생물학적 복합토양정화공정에 관한 연구)

  • Cha, Minwhan;Lee, Hanuk;Park, Jaewoo
    • Journal of the Korean GEO-environmental Society
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    • v.11 no.5
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    • pp.5-13
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    • 2010
  • The research is intended to develop and verify a biological complex soil treatment process to treat and restore soil and groundwater which is contaminated with oil, heavy metals, and nutrients through experiments with the series of treatment process such as bioreactor, rolled pipe type of contact oxidation system(RPS), and chemical processing system. 5 microbial strains were separated and selected through experiment, whose soil purification efficiency was excellent, and it was noted that anion- and nonion-series of complex agent was most excellent as a surfactant for effectively separating oils from soils. Method to mix and apply selected microbes after treating the surfactant in the contaminated soil was most effective. The removal efficiencies of total petroleum hydrocarbon (TPH)-contaminated soil about 5,000mg/L and above 10,000mg/L were approximatly 90.0% for 28 days and 90.7% for 81 days by soil remediation system and the average removal efficiencies of BOD, $COD_{Mn}$, SS, T-N, and T-P in leachate were 90.6, 73.0, 91.9, 73.8, 65.7% by the bioreactor and RPS. The removal efficiency was above 99.0% by chemical processing system into cohesive agents.

Segmentation Method of Overlapped nuclei in FISH Image (FISH 세포영상에서의 군집세포 분할 기법)

  • Jeong, Mi-Ra;Ko, Byoung-Chul;Nam, Jae-Yeal
    • The KIPS Transactions:PartB
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    • v.16B no.2
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    • pp.131-140
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    • 2009
  • This paper presents a new algorithm to the segmentation of the FISH images. First, for segmentation of the cell nuclei from background, a threshold is estimated by using the gaussian mixture model and maximizing the likelihood function of gray value of cell images. After nuclei segmentation, overlapped nuclei and isolated nuclei need to be classified for exact nuclei analysis. For nuclei classification, this paper extracted the morphological features of the nuclei such as compactness, smoothness and moments from training data. Three probability density functions are generated from these features and they are applied to the proposed Bayesian networks as evidences. After nuclei classification, segmenting of overlapped nuclei into isolated nuclei is necessary. This paper first performs intensity gradient transform and watershed algorithm to segment overlapped nuclei. Then proposed stepwise merging strategy is applied to merge several fragments in major nucleus. The experimental results using FISH images show that our system can indeed improve segmentation performance compared to previous researches, since we performed nuclei classification before separating overlapped nuclei.