• Restorative Dentistry and Endodontics
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• 제32권4호
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• pp.335-342
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• 2007

C형 근관은 복잡한 근관계의 형태로 인해 임상가가 미리 인지하지 못한 경우 근관치료 시 많은 어려움에 직면하곤 한다. 본 연구에서는 하악 제2대구치의 근관치료 환자에서 C형 근관의 형태별 빈도를 알아보고 방사선 사진 상 C형 근관계의 형태학적 특징과의 관련성을 비교 평가하고자 본 연구를 시행하였다. 1998년부터 2005년까지 조선대학교 치과병원 보존과에 내원한 227례의 하악 제2대구치 근관치료 환자의 의무기록지를 조사한 후 임상에서 근관치료 시 기록된 치수강저의 형태와 해당환자의 방사선 사진을 기초자료로 다음과 같은 결론을 얻었다. 하악 제2대구치 C형 근관은 한국인에서 많은 빈도를 보였으며, 근관치료 시 환자의 방사선 사진을 잘 활용하여 치근단의 형태와 원심근관의 위치를 주의깊게 관찰하면 C형 근관을 미리 예측할 수 있을 것으로 사료된다.

• Restorative Dentistry and Endodontics
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• 제39권2호
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• pp.104-108
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• 2014

Objectives: To evaluate the effect of passive ultrasonic agitation on the cleaning capacity of a hybrid instrumentation technique. Materials and Methods: Twenty mandibular incisors with mesiodistal-flattened root shape had their crowns sectioned at 1 mm from the cementoenamel junction. Instrumentation was initiated by catheterization with K-type files (Denstply Maillefer) #10, #15, and #20 at 3 mm from the working length. Cervical preparation was performed with Largo bur #1 (Dentsply Maillefer) followed by apical instrumentation with K-type files #15, #20 and #25, and finishing with ProTaper F2 file (Denstply Maillefer). All files were used up to the working length under irrigation with 1 mL of 2.5% sodium hypochlorite (Biodyn$\hat{a}$mica) at each instrument change. At the end of instrumentation, the roots were randomly separated into 2 groups (n = 10). All specimens received final irrigation with 1 mL of 2.5% sodium hypochlorite. The solution remained in the root canals in Group 1 for one minute; and ultrasonic agitation was performed in Group 2 for one minute using a straight tip inserted at 1 mm from working length. The specimens were processed histologically and the sections were analyzed under optic microscope (x64) to quantify debris present in the root canal. Results: The samples submitted to ultrasonic agitation (Group 2) presented significant decrease in the amount of debris in comparison with those of Group 1 (p < 0.05). Conclusions: The hybrid instrumentation technique associated with passive ultrasonic agitation promoted greater debris removal in the apical third of the root canals.

• 대한한의학방제학회지
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• 제19권2호
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• pp.109-118
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• 2011

Objectives : The Lycoris squamigera Maxim has been used traditionally for treatment of various diseases. However, the studies on the effect of Lycoris squamigera Maxim have not been carried out. In the present study, extract of Lycoris squamigera Maxim were tested for their anti-inflammatory and anti-oxidation effect. Methods : The anti-inflammatory effect of the various solvent extract was studied in lipopolysaccharide (lps)-treated mouse macrophage cells. RAW 264.7 cells were pre-incubated with Lycoris squamigera Maxim extracts for 4h and treated with $1\;{\mu}g/m{\ell}$ lps for 18h, and then the anti-inflammatory effects of extracts were determined. The anti-oxidation effect of extracts measured by DPPH method, reductive potential test, total phenolics test. Results : Extracted root's ethyl acetate layer showed a significant decrease in nitric oxide. And that layer (root's ethyl acetate extract) was showed decrease in TNF-${\alpha}$ concentration dependently. Separated from Root's ethyl acetate extract was fraction 1 has $0.1{\sim}5\;{\mu}M$ range, fraction 2 has $0.1{\sim}10\;{\mu}M$ range did not showed cytotoxicity. Anti-oxidation result as DPPH test showed the best was root ethyl acetate extract. Redusing power was made a comparison between fractions and standard. They were showed similar value. Fraction's total phenol containing result was better then standard. Conclusions : These results suggest that these extracts can be used as anti-inflammatory, anti-axidation materials.

• Journal of Periodontal and Implant Science
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• 제27권1호
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• pp.249-262
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• 1997

The present study investigates the effects of calcium sulfate graft on the periodontal healing in intrabony periodontal defects of dogs. Following the general anesthesia with 30mg/kg pentobarbital injected intravenously, the first premolar was extracted and full-thickness periodontal flap was elevated from the second premolar to the fourth premolar. The portion of premolars coronal to the alveolar crest was removed and mesial and distal roots were separated. Exposed root canals were sealed with Caviton and covered completely with flaps sutured. Following the healing period of 12 weeks, the surgical sited were uncovered and $4{\times}4mm$ intrabony defects were surgically created. Those defects with calcium sulfate graft following the root planing was designated as the test sites and those with flap surgery-only were designated as control sites. The animals were sacrificed after 8 weeks and the healing was histologically analyzed. The results were as follows. 1. No foreign body reaction or inflammation were observed in either groups. Calcium sulfate was completely resorbed in the test sites. 2. New cementum was observed coronal to the notch in both groups. Connective tissue fibers were oriented parallel to the root surface in the controls. Connective tissues were formed in large amount in the sites. 3. Test sites showed marked amount of new bone formation while the control sites showed minimal bone gain. 4. Root resorption was observed in coronal portions of th control Sites. The results suggest that calcium sulfate is a biocompatible graft material with a potential for new bone and cementum formation.

• Journal of Ginseng Research
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• 제44권4호
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• pp.552-562
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• 2020

Background: Ginsenosides are the unique and bioactive components in ginseng. Ginsenosides are affected by the growing environment and conditions. In New Zealand (NZ), Panax ginseng Meyer (P. ginseng) is grown as a secondary crop under a pine tree canopy with an open-field forest environment. There is no thorough analysis reported about NZ-grown ginseng. Methods: Ginsenosides from NZ-grown P. ginseng in different parts (main root, fine root, rhizome, stem, and leaf) with different ages (6, 12, 13, and 14 years) were extracted by ultrasonic extraction and characterized by Liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry. Twenty-one ginsenosides in these samples were accurately quantified and relatively quantified with 13 ginsenoside standards. Results: All compounds were separated in 40 min, and a total of 102 ginsenosides were identified by matching MS spectra data with 23 standard references or published known ginsenosides from P. ginseng. The quantitative results showed that the total content of ginsenosides in various parts of P. ginseng varied, which was not obviously dependent on age. In the underground parts, the 13-year-old ginseng root contained more abundant ginsenosides among tested ginseng samples, whereas in the aboveground parts, the greatest amount of ginsenosides was from the 14-year-old sample. In addition, the amount of ginsenosides is higher in the leaf and fine root and much lower in the stem than in the other parts of P. ginseng. Conclusion: This study provides the first-ever comprehensive report on NZ-grown wild simulated P. ginseng.

• Journal of Ginseng Research
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• 제44권4호
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• pp.563-569
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• 2020

Background: White ginseng consists of the roots and rhizomes of the Panax species, and red ginseng is made by steaming and drying white ginseng. While red ginseng has both polar and nonpolar ginsenosides, previous studies showed white ginseng to have only polar ginsenosides. Because nonpolar ginsenosides are formed through the manufacture of red ginseng from white ginseng, researchers have generally thought that nonpolar ginsenosides do not exist in white ginseng. Methods: We developed a simultaneous quantitative method for six nonpolar ginsenosides in white ginseng using reverse-phase high-performance liquid chromatography coupled with integrated pulsed amperometric detection. The nonpolar ginsenosides of white ginseng were extracted for 4 h under reflux with 50% methanol. Results: Using the gradient elution system, all target components were completely separated within 50 min. Nonpolar ginsenosides were determined in the rhizome head (RH), main root (MR), lateral root, and hairy root (HR) of 6-year-old white ginseng samples obtained from several regions (Geumsan, Punggi, and Kanghwa). The total content in the HR of white ginseng was 37.8-56.8% of that in the HR of red ginseng. The total content in the MR of white ginseng was 5.9-24.3% of that in the MR of red ginseng. In addition, the total content in the RH of white ginseng was 28.5-35.8% of that in the HR of red ginseng Conclusion: It was confirmed that nonpolar ginsenosides known to be specific components of red ginseng were present at substantial concentrations in the HR or RH of white ginseng.

• 한국자원식물학회지
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• 제37권1호
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• pp.11-21
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• 2024

본 연구에서 육계 추출물로부터 cinnamyl alcohol을 분리·정제하였다. 연구 결과, H3 분획100 ㎍/mL에서 부정근 형성율이 286.6%였으며, 표준품 cinnamyl alcohol 1 mM (134.2 ㎍/mL)의 형성율이 290%로 유사한 결과를 나타내었다. 따라서 육계 methanol 추출물에 함유되어있는 cinnamyl alcohol이 녹두의 부정근 형성을 유도하는 주된 화합물일 것으로 판단된다. 육계에서 동정한 cinnamyl alcohol은 현재 식품첨가제 및 향료로써 사용되고 있으므로 안전성이 이미 보장되어 있으며, 한약재로써 사용된 육계 잔류물을 활용하여 부정근 형성 유도물질을 추출한다면비용적인 측면에서 또한 긍정적인 요인으로 작용할 것으로 판단된다. 따라서 육계는 유기농 농자재로써 충분히 사용 가능할 뿐만 아니라 신규 천연 식물생장조절제의 개발용 소재로서도 활용 가능할 것으로 사료된다.

• 정보과학회 컴퓨팅의 실제 논문지
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• 제20권10호
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• pp.555-560
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• 2014

본 논문에서는 SW 분할 실행을 이용한 데스크탑 가상화 환경에서 데이터 보안 방법을 제안한다. 사용자에게 분리된 가상머신을 할당하는 가상화 환경에서는 하나의 가상머신에서 실행되는 어플리케이션이 다른 가상머신에서 실행되는 어플리케이션과 고립된다는 장점이 있지만, 어플리케이션 가상화 서비스를 위한 분할 실행 기술에서는 서버에서 실행되는 어플리케이션들이 서버의 루트 권한으로 실행되므로 사용자 사이의 고립을 지원하지 않아 데이터 보안에 문제가 발생한다. 본 논문에서는 이 문제를 해결하기 위해서 서버 스토리지 필터, 뷰어 필터 및 파일 매핑 테이블을 이용한 데이터 보안 방법을 제안한다.

• 생약학회지
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• 제4권4호
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• pp.193-203
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• 1973

The saponins of two- and four-year-old American ginseng plants (Panax quinquefolium L.) (Araliaceae) collected in July and September were studied. American ginseng saponins (panaquilins) differ from Korean ginseng $(Panax ginseng\;C.A.\;M_{EYER})$ saponins (ginsenosides). The American ginseng saponins separated and named were panaquilins A, B, C, D, E-1, E-2, E-3, G-1, G-2, (c) and (d). One-dimensional thin-layer chromatography did not completely separate panaquilin mixture and was subject to misinterpretation. The panaquilins were more accurately separated and identified by the two-dimensional thin-layer method established. Some differences in American ginseng saponins were dependent upon the plant age, time of collection, and part extracted. The American ginseng sapogenin components are panaxadiol (panaquilins B and C), oleanolic acid (panaquilin D) and panaxatriol (panaquilin G-1). The panaquilins E-1, E-2 and E-3 mixture contained both panaxadiol and panaxatriol. The genins of panaquilins A, (c), (d) and G-2 were not identified. In addition, ${\beta}-sitosterol$ and stigmasterol were identified from the root ether extracts.

• 대한소아치과학회지
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• 제31권1호
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• pp.92-97
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• 2004

청소년기에는 두개악안면부위의 외상성 손상이 흔하다. 그러나 상아질, 백악질, 치수를 포함하는 치아파절로 정의 되는 치근파절을 비교적 드물다. 성장기 아동에 있어서 외상으로 인한 손상은 심리적인 위축감, 안모에 대한 열등감 등의 정서적인 문제와 치조골과 안모의 성장이나 치간 간격의 유지에 영향을 미치는 등의 지장을 초래하기 때문에 보존적인 치료가 요구된다. 첫 번째 증례는 9세의 남아로 상악 우측 중절치의 치수노출을 동반한 치관 파절과 치근단부위에 치근의 수평 파절이 관찰되어 치관부 파절편 치수만 제거하고 수산화칼슘을 이용하여 충전하였다. 6개월 후 방사선 사진에서 dentinal bridge의 형성을 확인할 수 있었고 치근단부는 병적 소견 없이 정상적으로 발육되고 있었다. 1년 3개월 후 방사선 사진에서 치근단부의 형성이 완료된 것을 확인하여 gutta percha를 이용하여 영구 충전을 시행하였다. 두 번째 증례는 7세의 여아로 상악 우측 중절치의 치수 노출을 동반한 치근의 수직 파절이 관찰되어 인위적 발거 후 레진 시멘트를 이용하여 파절편 부착 후 재식하였다. 술 후 8개월 동안의 관찰결과 특이한 임상적 증상은 나타나지 않았다. 이상의 증례에서 치근 파절이 발생한 치아에서 보존적 치료를 시행하였고 이에 양호한 결과를 얻었기에 보고하는 바이다.