• 제목/요약/키워드: Seong-ryong Ryu

검색결과 53건 처리시간 0.033초

Proteomic analysis of rice mutants susceptible to Magnaporthe oryzae

  • Ryu, Hak-Seung;Song, Min-Young;Kim, Chi-Yeol;Han, Muho;Lee, Sang-Kyu;Ryoo, Nayeon;Cho, Jung-Il;Hahn, Tae-Ryong;Jeon, Jong-Seong
    • Plant Biotechnology Reports
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    • 제3권2호
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    • pp.167-174
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    • 2009
  • To identify genes involved in rice Pi5-mediated disease resistance to Magnaporthe oryzae, we compared the proteomes of the RIL260 rice strain carrying the Pi5 resistance gene with its susceptible mutants M5465 and M7023. Proteins were extracted from the leaf tissues of both RIL260 and the mutant lines at 0, 24, and 48 h after M. oryzae inoculation and separated by two-dimensional polyacrylamide gel electrophoresis (2-DE). Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) analysis identified eight proteins that were differently expressed between the resistant and susceptible plants (three down- and five up-regulated proteins in the mutants). The down-regulated proteins included a triosephosphate isomerase (spot no. 2210), a 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (no. 3611), and an unknown protein (no. 4505). In addition, the five up-regulated proteins in the mutants were predicted to be a fructokinase I (no. 313), a glutathione S-transferase (no. 2310), an atpB of chloroplast ATP synthase (no. 3616), an aminopeptidase N (no. 3724), and an unknown protein (no. 308). These results suggest that proteomic analysis of rice susceptible mutants is a useful method for identifying novel proteins involved in resistance to the M. oryzae pathogen.

멀티존 온돌난방제어를 위한 필요공급온수온도 산출법에 관한 연구 (A Study on the Required Supply Water Temperature Calculating Method for the Control of Multizone Radiant Floor Heating System)

  • 최정민;이규남;류성룡;김용이;여명석;김광우
    • 설비공학논문집
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    • 제19권1호
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    • pp.77-85
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    • 2007
  • One of the most common approaches to achieve better thermal comfort with the radiant floor heating system is supply water temperature control, which is that supply water temperature is varied with outdoor air temperature. But the application of this control method was not easy, because there has been no way to determine the supply water temperature. So in this study, a comprehensive, yet simple calculation method to find out the required supply water temperature is suggested by combining the building heat loss equation and the heat emission model of hydronic radiant floor heating system for single zone. And then using this calculation method, the multizone control method is suggested and confirmed through the thermal simulation. It is shown that indoor air temperature is stably maintained around the set point.

Preventive effects of nano-graphene oxide against Parkinson's disease via reactive oxygen species scavenging and anti-inflammation

  • Hee-Yeong Kim;Hyung Ho Yoon;Hanyu Seong;Dong Kwang Seo;Soon Won Choi;Jaechul Ryu;Kyung-Sun Kang;Sang Ryong Jeon
    • BMB Reports
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    • 제56권3호
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    • pp.202-207
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    • 2023
  • We investigated the neuroprotective effects of deca nano-graphene oxide (daNGO) against reactive oxygen species (ROS) and inflammation in the human neuroblastoma cell line SH-SY5Y and in the 6-hydroxydopamine (6-OHDA) induced Parkinsonian rat model. An MTT assay was performed to measure cell viability in vitro in the presence of 6-OHDA and/or daNGO. The intracellular ROS level was quantified using 2',7'-dichlorofluorescein diacetate. daNGO showed neuroprotective effects against 6-OHDA-induced toxicity and also displayed ROS scavenging properties. We then tested the protective effects of daNGO against 6-OHDA induced toxicity in a rat model. Stepping tests showed that the akinesia symptoms were improved in the daNGO group compared to the control group. Moreover, in an apomorphine-induced rotation test, the number of net contralateral rotations was decreased in the daNGO group compared to the control group. By immunofluorescent staining, the animals in the daNGO group had more tyrosine hydroxylase-positive cells than the controls. By anti-Iba1 staining, 6-OHDA induced microglial activation showed a significantly decrease in the daNGO group, indicating that the neuroprotective effects of graphene resulted from anti-inflammation. In conclusion, nano-graphene oxide has neuroprotective effects against the neurotoxin induced by 6-OHDA on dopaminergic neurons.

경북 지역의 향사와 불천위제례의 진설과 제수 비교 - 대구와 안동지역 중심으로 - (Comparison of Hyang-Sa and Bulchunwee Rituals and Food in Kyungbuk - Focused on Daegu and Andong Areas -)

  • 최정희;박금순
    • 한국식품조리과학회지
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    • 제24권6호
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    • pp.801-810
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    • 2008
  • The principal objective of this study was to assess Korea's traditional ritual food culture, and to compare two types of ancestral rites the Hyang-Sa and Bool-cheon-wi rites which were held in the Daegu and Andong regions of Korea. In this study, we describe the performance of the Bool-cheon-wi rites held by two head families located near the Andong area namely, the head family of Seoae Ryu Seong-Ryong(1542-1607)(Seoae) who was well-respected for his writings and personality, and the head family of Dangye Ha Wee-Ji(1412-1456)(Dangye) who was well-known and famous as one of the members of the Sa-ryuk-sin. This research was conducted via diredt engagement in these memorial services and several interviews with the families. The results were summarized as follows; Foods used in the Hyang-sa rites including Mae(bap), Kook, Jaban(Jogee), Po(dried fish), Juck, boiled and seasoned vegetables, fruits, confections, and liquor. Jobap and Ssalbap were used as Mae at SD(Seo Dalsung), and PMPH(Pahoi Myogul Habin Park) used Jo, Hyunmy, Pi and Susu in the raw. The dishes on the table were arranged as follows. A wine cup was placed in the first row, Po(a dried pollack), and jujube and nuts in the second row, Ryukpo(slices of dried beef), Sangeogogi were set at the third row, and Soegogi, pork, Mu, and Minary were placed in the fourth row, and the head of the pig was placed in the center of the table at SD. A wine cup, Soegogi, and pork were positioned in the first row, Mu, Minary, Pi and Susu were placed in the second row, and Jogee, Jo, and Hyunmy were placed in the third row at PMPH. The sacrificial foods offered for Bool-cheon-wi rites were as follows; Mae(bap) Kook noodle Jogee Tang(stew) Po Juck Tucks boiled, seasoned and salted vegetables Jeon fruit confectioneries liquor(chungju). The head family of Seoae Yu Seong-Ryong utilized 5 types of Tang(meat stew, fish stew, chicken stew, vegetable stew, seafood stew), whereas the head family of Dangye Ha Wee-Ji utilized 3 kinds of Tang(meat stew, fish stew, vegetable stew). As a basic Tuck, Shi-roo-tuck(a steamed rice cake), piled up to 25 layers, was primarily utilized. In particular, Jung-Gae(Seoae's favorite food) was placed on the table. For grilled-meat food(Juck), Yu's family used raw meat and Ha's family the half-cooked meat. The main types of Jucks used were meat-Juck, fish-Juck, chicken-Juck, and these were not served one by one. Hyang-sa and Bool-cheon-wi rites have an the educational function, in that they allow for the carrying out of filial duties by a heartfelt performance of performing the ceremony, by taking great care from the pre-rite preparations to the post-rite period. In addition, these rites have various meanings, as events that strengthen the ties of blood relations of ancestors and themselves, and to promote and harmonize family friendships, they may also have religious meaning in the culture, as prayers are offered that all the family's descendants may be blessed, live long and enjoy abundance whlie respecting their ancestors. As for the role of Hyang-sa and Bool-cheon-wi rites in today's nuclear family society, it can be said that these rites remain especially important as a method to strengthen community consciousness by fostering an understanding of the meaning of existence itself, and thus inspiring the roots of consciousness.

퇴행성관절염(退行性關節炎) 한방치료(韓方治療)에 대(對)한 최근(最新) 연구(硏究) 동향(動向) - 임상연구(臨床硏究) 방법론(方法論)을 중심(中心)으로 - (Systemic review: Herbal Medicines in the Treatment of Osteoarthritis in Pubmed and Chinese Medical Journals)

  • 서병관;류성룡;이송실;허정은;백용현;이재동;최도영;조윤제;김남재;박동석
    • Journal of Acupuncture Research
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    • 제21권3호
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    • pp.265-282
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    • 2004
  • Objective : The aim of this study was to review systemically clinical trials on the effectiveness and safety of herbal medicines in the treatment of osteoarthritis. Methods : Computerized literature searches were carried out on seven electronic databases, and hand-searching on some chinese medical journals in library of Kyung Hee Medical Center. Trial data were extracted in a standardized, predefined manner and assessed independently. Results : 1. Thirty reports of clinical trials and two reports of meta-analyses concerning herbal medicine were collected and reviewed. Among these reports three medical herbs were applied as topical medicine and others as internal medicine. 2. The western studies established NSAIDs or placebo as their control group. Five chinese reports established formulated herb pill(Ruanshnagshenjin pill) as their control group and Six did not establish a control group at all. 3. ACR was the most highly used diagnostic criteria in the western studies while the Chinese used their official criteria established by their government or the criteria of their text books. 4. 20 reports chose the Lequesne functional index, SHAQ, WOMAC OA index, AIMS, and their own unique scoring system as the criteria of analysing the effect. Others chose clinical symptoms, articular functions, and lab finding as their criteria. 5. 7 single herbs and 19 formulated herbs were studied. Among the formulated herbs, Achyranthes japonica was studied in 10 of the studies and Angelica gigantis Radix in 8, making them the most often studied herbs among the studies.

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고출력 LED 패키지용 고밀도 W-20wt%Cu 나노복합체 제조에 관한 연구

  • 류성수;박해룡;김형태;이병호;이혁;김진우;김영도
    • 한국재료학회:학술대회논문집
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    • 한국재료학회 2010년도 춘계학술발표대회
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    • pp.26.2-26.2
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    • 2010
  • 최근에는 차세대 조명용 후보광원인 고출력 백색 LED를 개발하기 위한 경쟁이 치열하며, 이를 위해 업체가 고심하고 있는 가장 큰 문제 중의 하나가 칩에서 발생하는 열을 어떻게 관리하는가 하는 방열의 문제이다. 따라서, LED의 가장 큰 특징인 장수명을 손해보지 않기 위해서는 칩에서 발생되고 있는 열을 외부에 확산시키기 위한 기술 개발이 필수적이다. 다양한 방열소재 중 W-Cu 복합재는 W의 낮은 열팽창계수와 Cu의 높은 열전도도로 인해 방열소재로써 유망한 소재로 주목받고 있으나, 우수한 열적 특성을 발현하기 위해서는 고치밀화를 갖는 W-Cu 복합재 제조가 우선적으로 필요하다. W-Cu 복합체는 일반적으로 액상소결법을 통해 균일한 미세조직을 얻을 수 있으나, 열팽창계수를 낮추기 위해 Cu 함량이 적어지게 되면 치밀화가 어려우며 이를 해결하기 위해 나노입자를 갖는 분말을 이용하고자 하는 연구가 많이 진행되고 있다. 본 연구에서는 W과 Cu 산화물을 이용하는 것이 구성성분끼리의 편석이 발생하지 않으며, 소결성도 우수하여 양산화에 가장 접근한 방법으로 알려져 있다. 그러나, 지금까지의 얻어진 W-Cu 복합체의 경우, 분말상태에서의 얻어진 나노입자가 승온시에 마이크로 크기로 과도한 입자성장이 일어나기 때문에 소결 후에도 나노크기를 유지하기 어려울 뿐만 아니라, 구성상끼리의 응집체가 형성된다. 본 연구에서는 액상소결후에 W 입자가 Cu 기지내에 균일하게 분산되는 동시에 나노크기의 입자를 가지는 고분산 W-Cu 소결체를 얻고자 하였다. 이를 위해 금속산화물 분말의 분쇄를 위해 효과적인 방법으로 알려진 습식상태에서의 고에너지 볼밀링을 통하여 혼합된 텅스텐과 구리 산화물 분말의 수소환원공정을 통해 얻어진 100nm 이하의 입자를 가지는 W-20wt%Cu 나노복합분말을 출발분말로 사용하였다. W-20wt%Cu 나노복합분말의 성형체를 $1050^{\circ}C-1250^{\circ}C$의 온도범위에서 소결거동을 조사하였다. 그 결과, $1100^{\circ}C$ 온도에서 이론밀도에 가까운 소결밀도를 나타내었으며, 이는 기존에 비해 $100^{\circ}C$ 정도 치밀화 온도를 낮추는 결과이다. 소결체의 미세구조 관찰결과, 소결 후 약 200nm의 텅스텐 입자가 Cu내에 균일하게 분산되어 있었다. 제조된 W-Cu 시편에 대해서는 LED 응용성을 조사하기 위해 열전도도와 열팽창계수 등을 평가하였다.

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퇴행성 슬관절염 환자의 증상 중증도 지표와 DITI의 상관성 연구 (Study on the Applicability of Thermography as Severity Measurement in the Patients with Osteoarthritis of the Knee)

  • 서병관;류성룡;강중원;안경애;이재동;최도영;김건식;이두익;이윤호;이상훈
    • Journal of Acupuncture Research
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    • 제22권4호
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    • pp.35-45
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    • 2005
  • Objectives : To investigate the applicability of thermography as severity measurement in the patients with osteoarthritis (OA) of the knee. Methods : Data were obtained from 80 patiens with OA of the knee. They were asked to answer two disease-specific questionnaire (Western Ontario and McMaster Universities (WOMAC) OA index. Lequesne's Functional Index (LFI)), one generic instrument (Korean Health Assessment Questionnaire (KHAQ)), VAS in order to assess the severity of disease, quality of life, and degree of pain and taken thermography in standardized environment. Results : The thermal difference between ipsilateral side and contralaterla side of lateral aspect of knee was correlated with that of medial aspect of knee, and the thermal difference of anterior thigh was correlated with that of lateral, medial aspect of knee and patella region. Age, duration of disease, duration of morning stiffness, sex, crepitus, and painful side of knee were not correlated with the thermal differences of each region. LFI, WOMAC, WOMAC pain subscale, WOMAC stiffness subscale, WOMAC physical function, KHAQ, VAS were not correlated with the thermal differences of each region. Conclusion : Futher study on the thermography on OA of the knee in population with appropriate severity grade and the standardization of analysis of thermographic data were recommended.

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퇴행성 슬관절염 환자 평가 설문도구의 내적 신뢰도, 상관성에 관한 연구 (The Study about Internal Reliability and Correlation among Assessment Instruments of Knee OA)

  • 류성룡;안경애;서병관;강중원;이상훈;이재동;최도영;김건식;이두익;이윤호
    • Journal of Acupuncture Research
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    • 제22권3호
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    • pp.271-280
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    • 2005
  • 2005년 1월 1일부터 3월 1일 까지 신문과 방송 그리고 경희의료원 홈페이지를 통해 모집된 퇴행성 슬관절염 핀자에 VAS, LFI, WOMAC, KHAQ 의 설문을 시행하여 다음과 같은 결론을 얻었다. 1. LFI, WOMAC Pain Subscale, WOMAC Function Subscale, WOMAC Total Score, KHAQ 모두 Cronbach`s alpha가 0.70이상 (p=.000)으로 내적 신뢰도가 높았다. WOMAC Stiffness Subscle 만이 Cronbach`s alpha가 0.6679(p=.000)이나 이는 문항수가 작기 때문이다. 2. LFI, WOMAC 각 항목, WOMAC Total Score, KHAQ는 모두 Pearson correlation coefficient가 0.612(p=.000)이상으로 높은 상관성을 보였으며, 이중 LFI와 WOMAC Pain Subscale의 상관관계가 비교적 높았다. 이는 통증이 관전기능에 큰 영향을 미치며, 관절염 증상의 악화는 곧 삶의 질에 큰 영향을 미친다는 것을 의미한다. 3. 관절염에 의한 통증, 강직, 관절기능의 제한은 환자의 삶의 질에 큰 영향을 미치게 되므로 관절염의 치료는 통증조절 등의 대증요법이외에 환자의 삶의 질에 초점을 두어야 하고 이를 위해 적절한 설문도구를 활용이 필요하다.

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플라스틱 온실 죔쇠의 미끄럼 저항력 평가를 위한 실험 방법 (Test Methods for Evaluation of Slip-Resistance Force of Clamp for Plastic Greenhouse)

  • 최만권;송호성;조명환;유인호;신영안;임성윤;류희룡
    • 생물환경조절학회지
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    • 제25권3호
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    • pp.193-199
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    • 2016
  • 본 연구에서는 플라스틱 온실에서 사용하는 죔쇠의 성능 평가 시 시험편 끝단의 구속조건이 미끄럼 저항력에 미치는 영향을 분석하기 위해 시험편 구속조건을 실험변수로 판형죔쇠의 미끄럼 저항력을 측정하였다. 각 구속조건별로 측정한 미끄럼 저항력의 평균값은 서까래 고정-고정단이 가장 컸으며, 내재해형 죔쇠의 평가 기준 값과 비교한 결과에서도 서까래 고정-고정단의 경우에만 기준을 만족하는 것으로 나타났다. 그러나 서까래 고정-고정단 구속조건은 다른 구속조건들에 비해 산포도가 상대적으로 크게 나타나 신뢰성이 떨어지는 결과를 보였다. 또한, 일원분산 분석결과에서도 서까래 고정-고정단은 다른 구속조건들과 비교해 미끄럼 저항력이 유의한 차이를 보였다. 따라서 시험편 구속조건이 미끄럼 저항력 평가에 영향을 미친다고 판단하며 연구자 및 기술자들이 죔쇠의 미끄럼 저항력을 측정할 때에는 시험편의 구속조건이 결과값의 신뢰성에 미치는 영향을 면밀히 검토해야 할 것으로 판단된다.

측정방법에 따른 노동시간의 차이: 자기기입식 질문법과 시간일지법을 중심으로 (The Discrepancy of Work Time according to the Measures: Self-reported Questions vs. Time-diary Method)

  • 유성용
    • 한국인구학
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    • 제31권1호
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    • pp.99-125
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    • 2008
  • 본 논문에서는 자기 기입식 질문법에 의한 평가 노동시간과, 시간일지법에 의한 일기 노동시간 간에, 노동시간의 길이에 의한 체계적인 편차가 존재하는지 밝히고자 하였다. 노동시간의 길이의 측정 및 계산에 있어서, 자기 기입식 질문법은 그 측정의 용이성 등의 장점에 의해 주로 사용되어 온 반면, 시간일지법은 응답자의 행동들을 그 연속성 하에서 기록함으로써 보다 실제에 가까운 생활시간을 구할 수 있다는 장점에 의해 사용되어 왔다. 2004년도 생활시간조사 자료를 이용해 분석한 결과, 평가 노동시간을 기준으로 했을 때에는 평가 노동시간이 증가함에 따라 노동시간의 과대평가의 경향이 강해진다는 결론이 나오는 반면, 일기 노동시간을 기준으로 했을때에는 일기 노동시간이 증가함에 따라 노동시간의 과소평가의 경향이 강해진다는 결론이 나오는, 모순으로 보이는 결과가 나왔다. 공통된 조사에서 나온 자료임에도 불구하고 서로 정반대의 결과를 나타내는 이유는 평균에의 회귀 현상에 의해 무작위 오차가 서로 반대의 방향으로 작용 하기 때문이다. 따라서 평가 노동시간을 기준으로 한 결과만을 근거로 장시간 노동을 하는 사람 일수록 자신의 노동시간을 과대평가하는 경향이 강하다고 주장할 수는 없다. 즉, 노동시간의 증가에 따른 체계적인 편차는 존재하지 않는다는 사실이 확인되었으며, 따라서 이러한 편차의 존재를 근거로 자기 기입식 질문법에 의한 평가 노동시간을 통해 구해진 자료들의 신뢰성에 의문을 제기하는 것 역시 불가능하다.