• Horticulture, Environment, and Biotechnology : HEB
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• 제59권6호
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• pp.815-826
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• 2018

The intense drought affecting olive production in Northern Chile underscores the need to research non-traditional irrigation strategies to obtain the best crop performance. Accordingly, this study aimed to obtain preliminary data to guide future research on this topic. Different water replenishment levels on crop evapotranspiration ($ET_c$ ; 13.5, 27.0, 40.5, and 54%) were established in a young orchard, cv. Arbequina, from the end of fruit drop (EFD) to full bloom in the next season. We evaluated the influence of plant water status (${\Psi}_{stem}$ ) and crop load, considered as function of fruit number divided by trunk cross-sectional area, on reproductive and productive variables using multiple linear regressions. Our results show that crop load and ${\Psi}_{stem}$ measured from EFD to harvest affected yield components. Nevertheless, ${\Psi}_{stem}$ had the strongest influence on fruit size, pulp development, oil accumulation, and yield. Oil content and yield were reduced by 54% and 50% for each MPa, respectively, from ${\Psi}_{stem\;EFD-H}$ -1.8 MPa, an effect that intensified as crop load increased. During the period of flower development (September-November), the number of flowers per inflorescence and percentage of perfect flowers were reduced when ${\Psi}_{stem}$ was less than -2.0 MPa. These preliminary results showed that bud differentiation, inflorescence and flower formation are highly sensitive to water deficit.

• Geomechanics and Engineering
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• 제30권4호
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• pp.373-382
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• 2022

Unsaturated soil at high suctions is widespread. Many civil engineering projects are related to the hydro-mechanical behavior of unsaturated soils at high suctions, particularly in arid and semiarid areas. To investigate water retention behaviors of nine clayey soils (one is classified as fat clay and the others are classified as lean clay according to the unified soil classification system), the high suction (3.29-286.7 MPa) was imposed on the specimens at zero net stress by the vapor equilibrium technique. In this paper, the effect of void ratio on the water retention behavior at high suction was discussed in detail. Validation data showed that soil types, i.e., different mineralogical compositions, are critical in the soil water retention behavior at a high suction range. Second, the hysteresis behavior at a high suction range is mainly related to the clay content and the specific surface area. And the mechanism of water retention and hysteresis behavior at high suctions was discussed. Moreover, the maximum suction is not a unique value, and it is crucial to determine the maximum suction value accurately, especially for the shear strength prediction at high suctions. If the soil consists of hydrophilic minerals such as montmorillonite and illite, the maximum suction will be lower than 10⁶ kPa. Finally, using the area of hysteresis to quantify the degree of hysteresis at a high suction range is proposed. There was a good correlation between the area of hydraulic hysteresis and the specific surface area.

• Journal of Microbiology and Biotechnology
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• 제27권1호
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• pp.179-188
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• 2017

White-rot basidiomycetes are the organisms that decompose lignin most efficiently, and Trametes villosa is a promising species for ligninolytic enzyme production. There are several publications on T. villosa applications for lignin degradation regarding the expression and secretion of laccase and manganese peroxidase (MnP) but no reports on the identification and characterization of lignin peroxidase (LiP), a relevant enzyme for the efficient breakdown of lignin. The object of this study was to identify and partially characterize, for the first time, gDNA, mRNA, and the corresponding lignin peroxidase (TvLiP) protein from T. villosa strain CCMB561 from the Brazilian semiarid region. The presence of ligninolytic enzymes produced by this strain grown in inducer media was qualitatively and quantitatively analyzed by spectrophotometry, qPCR, and dye fading using Remazol Brilliant Blue R. The spectrophotometric analysis showed that LiP activity was higher than that of MnP. The greatest LiP expression as measured by qPCR occurred on the $7^{th}$ day, and the ABSA medium (agar, sugarcane bagasse, and ammonium sulfate) was the best that favored LiP expression. The amplification of the TvLiP gene median region covering approximately 50% of the T. versicolor LPGIV gene (87% identity); the presence of Trp199, Leu115, Asp193, Trp199, and Ala203 in the translated amplicon of the T. villosa mRNA; and the close phylogenetic relationship between TvLiP and T. versicolor LiP all indicate that the target enzyme is a lignin peroxidase. Therefore, T. villosa CCMB561 has great potential for use as a LiP, MnP, and Lac producer for industrial applications.

• Asian-Australasian Journal of Animal Sciences
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• 제16권5호
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• pp.655-659
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• 2003

Forty eight lambs (24 each of Malpura and Mutton synthetic breeds) were weaned at 90 days of age and divided into two groups. One group of 12 lambs from each of the two breeds was maintained on low energy (52% TDN) and the other group of 12 on high energy (58% TDN) feedlot ration until 6 of the lambs attained 20 kg and the other 6 attained 25 kg live weight in each of the two groups. Daily feed intake and weekly body weights were recorded. Conventional metabolism trials were conducted on the two breeds and the two rations. The growth rate was found to be higher for lambs on high energy ration (p<0.01). The growth rate was also higher for higher target weight groups (p<0.01). The breed did not significantly affect the live weight gains. Mutton synthetic lambs required less number of days to reach 20 kg live weights but took more time to reach 25 kg target weight as compared to Malpura lambs (p<0.01). The lambs of both the breeds reached 25 kg live weight earlier on high energy than those on low energy ration (p<0.01). The dry matter intake, irrespective of breed, was significantly higher (4.57% or 93.4 g per kgW$^{0.75}$) on low energy than that on high energy ration (4.20% or 87.2 g per kg W$^{0.75}$). The digestibility coefficients of all the nutrients excepting crude fibre were significantly higher on high energy diet irrespective of the breed. Lambs on low energy ration, however, digested the crude fibre more than those on the high energy ration (p<0.05). Although there were no significant differences in the intakes of nitrogen, calcium and phosphorus, the balance of nitrogen was higher on high energy ration. The DCP and TDN values were 9.70 and 52.76% for low energy and 9.89 and 57.68 % for high energy ration, respectively. The dressing percentages on live weight basis were 50.2 on low and 51.6 on high energy ration, 50.5 at 20kg and 51.3 at 25 kg slaughter weight and 51.0 in Malpura and 50.8 in Mutton synthetic lambs, respectively. The percent of bones in the carcass was higher on low energy ration in Mutton synthetic lambs at 20 kg slaughter weight than others. It was concluded that the performance of the lambs in respect of mutton production was significantly better on high energy ration fed upto 25 kg slaughter weight with no or little breed differences.

• 한국환경생태학회지
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• 제28권2호
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• pp.161-170
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• 2014

본 연구는 석회암이 나출되어 있는 덕항산지역 주요 산림식생을 중심으로 그 유형을 분류하고 군락지리적 특성을 분석하였다. 덕항산 능선부 일대 산림식생의 유형은 1개 군락군(신갈나무군락군), 3개 군락(개회나무-조록싸리군락, 소나무-철쭉군락, 소사나무-솔체꽃군락), 2개 군(소사나무-회양목군, 까치박달-사창분취군), 2개 소군(들메나무-부채마소군, 털댕강나무-솔나리소군)으로 총 5개 유형으로 구분되었다. 덕항산 군락지리형은 지형적 특이성, 아고산지대와의 인접성, 석회암 지대 등의 특징을 잘 나타내고 있다. 그리고 배수가 잘되는 약건조지에 출현하는 털대사초, 가는잎그늘사초 등의 고상재도는 석회암지대의 토양환경을 잘 대변해 주고 있으며 북방계 식물인 싸라기사초, 돌좀고사리 등은 인접한 아고산 식생의 특성을 보여주고 있다. 그리고 소사나무의 출현은 서식지 특이성에 의한 피난식생 특징을 나타내고 있는 것으로 생각된다. 본 연구 지역의 군락지리학적 특이성은 서식지 및 식물지리적 특이성이 결합되어 나타나고 있다. 그리고 식생학적 측면에서 한반도 군락 및 서식지 유형의 다양성 증진에 기여할 것으로 생각된다.

• Asian-Australasian Journal of Animal Sciences
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• 제15권12호
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• pp.1719-1724
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• 2002

An investigation was made to evaluate the native range of western India in terms of nutrient supply during different physiological stages in goats. One hundred female and 5 male Kutchi adult goats were grazed on a 35 ha plot of native range. They were maintained on sole grazing. Four experiments, one in a defined physiological stage was carried out. Stage I, when maximum number of females were bred; stage II, when bucks were used for breeding purpose; stage III, 3-months old (weaner) kids were allowed to graze on ranges and stage IV, when the goats were in early stage of lactation. During oestrous period, diet contained 15.2% crude protein (CP), 54.0% neutral detergent fibre (NDF), 31.5% acid detergent fibre (ADF) and 12.6% acid detergent lignin (ADL). Digestibility coefficient of dry matter (DM), CP, NDF and ADF was 0.586,0.531, 0.431 and 0.239, respectively. DM, digestible crude protein (DCP) and metabolizable energy (ME) intakes were $82.7,\;6.71g\;kg^{-1}\;W^{0.75}$ and $0.99MJ\;kg^{-1}\;W^{0.75}$. Diet of buck was constituted by $230g\;kg^{-1}$ DM of grasses and forb and $770g\;kg^{-1}$ DM of tree foliage. CP, NDF, ADF and ADL contents of the diet were 13.6, 57.8, 43.5 and 20.0%, respectively. DM, DCP and ME intakes were $57.9,\;4.13g\;kg^{-1}\;W^{0.75}$ and $0.82MJ\;kg^{-1}\;W^{0.75}$. Digestibility coefficient of CP, NDF and ADF were 0.496, 0.432 and 0.346, respectively. Diet of kids was constituted by Prosopis cineraria ($900g\;kg^{-1}DM$) and Zizyphus nummularia ($100g\;kg^{-1}DM$) leaves. Digestibility of CP, NDF and ADF were 0.456, 0.415 and 0.201, respectively. DM, DCP and ME intakes were $85.8,\;6.44g\;kg^{-1}\;W^{0.75}$ and $1.22MJ\;kg^{-1}\;W^{0.75}$. Lactation phase of goat fell between October-December and tree foliage formed the major portion of the diet. Diet of lactating goat contained only 6.8% CP and 16.5% ADL. Digestibility of DM, CP, NDF and ADF was 0.567, 0.221, 0.307 and 0.233, respectively. DM, DCP and ME intakes were $108.2,\;1.62g\;kg^{-1}\;W^{0.75}$ and $1.50MJ\;kg^{-1}\;W^{0.75}$, respectively. Present study establishes that goats on semiarid range with sufficient number of fodder trees were able to meet the nutrient requirement of ewes during oestrous and bucks during breeding season. However, during lactation and active growth phase, only DM requirement is met. Thus, it is recommended to supplement concentrate mixture to goats grazing on range during lactation and growth phase.

• Asian-Australasian Journal of Animal Sciences
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• 제31권4호
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• pp.529-536
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• 2018

Objective: Fresh sugarcane has been a new roughage source for ruminant's in semiarid regions, a function of the decline of sugar and alcohol industry in recent years. However, there is little data published regarding lambs fed sugarcane associated with spineless cactus. This study evaluated the effect of sugarcane replacement with spineless cactus (0%, 33%, 66%, and 100%) in the diet of Santa $In\hat{e}s$ lambs on carcass characteristics. Methods: Thirty-six non-castrated Santa Ines lambs at four months of age and an initial body weight of $22{\pm}2.3kg$ were assigned in a randomized block design and slaughtered after 70 days of confinement. The effects of spineless cactus as a replacement for sugarcane in the diet of the lambs on the carcass characteristics, commercial cut weight and yield, leg tissue composition, and carcass measurements were studied. Results: The study revealed quadratic behavior in slaughter body weight, and hot and cold carcass weight, with maximum values of 38.60, 18.60, and 18.11 kg and replacement levels of 40.18%, 44.42%, and 43.14%, respectively. The cold carcass yield presented an increasing linear behavior. The compactness index of carcass and leg presented a quadratic effect, with estimated maximal values of 0.28 and 0.57 kg/cm and replacement levels of 43.37% and 45.5%, respectively. The weights of commercial cuts of leg, loin, shoulder, and breast showed quadratic behavior, with maximum values of 2.79, 0.852, 1.46, and 1.30 kg and replacement levels of 49.5, 45.32, 39.0, and 40.7, respectively. For tissue composition, quadratic behavior was verified for leg weight, subcutaneous fat, and total fat. Conclusion: The replacement of sugarcane by spineless cactus at level 44% is recommended for finishing lambs considering that this level improved most of the carcass characteristics, weights, and yields of commercial cuts and leg tissue composition.

• 한국조경학회지
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• 제41권2호
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• pp.1-10
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• 2013

이 연구는 서부 영화에서 황야를 재현하는 방식과 그 과정에서 발견되는 미학적 특성을 해석한다. 서부 영화는 자연 경관을 본격적으로 다룬 최초의 영화 장르로서, 시각 재현 매체가 자연 경관을 재현하는 방식의 원형을 보여준다. 서부 영화는 먼저 동부의 황야를 배경으로 제작되다가 이후에 서부의 황야를 포착하였고, 경관의 물리적 특징에 적합한 재현 방식을 통해 독특한 미적 특질을 조직하였다. 서부 영화에서 황야는 카메라에서 먼 거리에 위치하여 시각적 관조의 대상으로 재현되었다. 동부의 숲 경관의 재현은 허드슨 강 화파의 회화 양식의 영향을 받았고, 초월적 숭고의 미학을 구현하였다. 서부의 반건조지대의 재현에서는 높은 지대에서 아래를 내려다보는 하향적 시선이 등장하였고, 이때의 황야는 버크가 정의한 숭고의 특질을 보여준다. 서부의 사막은 한편으로 지면 높이에서 포착되어 지평선이 부재하고 무한함이 강조됨으로써 불모지로서의 황야를 표상하였다. 다른 한편으로 모뉴먼트 밸리는 장엄한 규모와 오랜 시간성의 측면에서 숭고함의 범주에 포섭되고, 광활한 사막에 지평선과 함께 시각적 상징물로서 재현되었다. 서부 영화의 황야 재현 방식에서는 안전한 거리를 두고 경관을 관찰하려는 인간의 생물학적 욕구인 조망과 은신의 관계가 발견되고, 이러한 점에서 서부 영화는 자연 경관의 구성 방식에 관한 원형을 보여준다고 할 수 있다.

• 한국지형학회지
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• 제20권4호
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• pp.15-28
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• 2013

본고는 2012년 11월 14일부터 25일까지 10일간 남미대륙의 파타고니아와 아타카마 지역을 중심으로 한 안데스 산지를 답사한 후 그곳에서 관찰된 지형경관에 대해 지오투어리즘 관점에서 고찰한 것이다. 파타고니아 지형경관은 칠레의 토레스델파이네 국립공원과 아르헨티나의 로스 빙하국립공원에서, 그리고 아타카마 지형경관은 칠레의 산페드로데아타카마를 중심으로 한 지역에서 주로 관찰되었다. 파타고니아는 세계에서 가장 빠른 융기율을 나타내고 있는 곳 중의 한 곳으로 위의 두 개 국립공원에는 반건조지형과 융기지형 그리고 빙식지형이 매우 잘 발달되어 있다. 특히, 파이네와 피츠로이 암봉군은 관입에 의한 화강암 빙식지형의 백미를 보여주고 있어 두 곳 모두 유네스코 세계자연유산으로 등록된 곳이기도 하다. 한편, 아타카마 사막은 악지와 염호, 간헐천 등 사막과 화산경관이 공존해 있는 독특한 지형경관이 발달된 곳이다. 그 이유는 아타카마 사막이 산페드로데아타카마 동쪽의 화산호(弧)와 해성층의 융기로 형성된 대륙붕단 사이의 전호분지에 위치하고 있기 때문이다. 또한 응결응회암 완사면, 선상지면 등으로 형성된 페디플레인이 곳곳의 페디멘트와 어우러져 건조지형의 전형을 잘 나타내 주고 있기도 하다. 파타고니아와 아타카마는 남미판과 태평양판의 충돌로 인해 형성된 안데스 산지가 만든 매우 다양한 지형경관이 발달된 최적의 지리여행지이다.

• 한국작물학회지
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• 제59권4호
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• pp.463-469
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• 2014

유색 수수인 황금찰수수를 도정할 때 grain과 함께 발생하는 도정부산물인 hull과 bran의 4가지 용매 추출물의 총 폴리페놀 함량, 항산화 활성, 천연색소 함량과 RAW 264.7 세포주를 이용하여 항염증 활성을 검정하였으며 결과를 요약하면 아래와 같다. 1. 도정부산물인 hull의 100% 메탄올과 70% 에탄올 추출물에서 총 폴리페놀 함량이 $29.4{\pm}0.4$와 $29.7{\pm}0.2mg\;GAE/100g$으로 가장 많았고, ABTS 라디컬 소거능($IC_{50}$)이 $6.8{\pm}0.4$, $6.3{\pm}0.1{\mu}g/mL$로 가장 높은 항산화 활성을 나타내었다. 2. 총 천연색소 함량에서도 수수 hull의 0.5% 염산 함유 100% 메탄올 추출물에서 $322.6{\pm}14.5mg/100g$으로 가장 많은 색소 함량을 나타내었고 hull, bran, grain 모두의 경우에서 0.5% 염산을 함유한 100% 메탄올을 용매로 추출한 경우에서 가장 많아 수수를 이용하여 천연색소를 추출할 경우 수수 부산물인 hull을 산을 함유한 메탄올로 추출하였을 때 가장 많은 색소 추출물을 얻을 수 있었다. 3. 수수 도정부산물과 grain 추출물을 LPS로 NO 생성을 유도한 RAW 264.7 세포에 처리하였을 때, 세포 독성이 없는 100 ug/mL 농도 이하에서의 NO 생성 억제량은 hull, bran, grain 추출물의 순으로 나타났고 hull 추출물의 경우에 농도에 따라 가장 큰 NO 생성 억제를 보였으며 천연색소 화합물 중에서는 apigeninidin의 NO 생성 억제 활성을 확인하였다. 4. 위 결과를 종합해 볼 때, 수수 도정부산물 중 hull은 grain에 비해 총 폴리페놀 화합물과 천연색소 함량이 높고 항산화 활성과 RAW 264.7 대식세포에서의 NO 생성량 억제 활성이 우수하여 앞으로 수수 부산물이 항산화 및 항염증 소재로 가능성이 있음을 확인하였다.