• The Journal of Korean Physical Therapy
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• 제10권1호
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• pp.229-239
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• 1998

The purpose of this article is to identify real problems and to have a view of the future in Korean physical therapy of it through the survey. Moreover we make a statement for the justification acquiring the rights of practing Physical therapiests with this fundamental data. The survey was conduced on 368 employed physical therapiests males 164. tamale 190 in korea from April t to 30 in 1998 with self-administered questionnaire. The following results were obtained : 1. Of the 323 cases, $121(37.46\%)$ work in local clinics, $55(17.02\%)$ in hospitals, $103(31.89\%)$ in general hospital and $21(9.60\%)$ in rehabilitation centers or public welfare facilities. 2. In investigation of annual salary, the most common annual salary was more than 20million won in male$(22.61\%)$ and $12\~14$million won in female$(35.36\%)$ 3. The age group of $27\~29$ years accounted for $32.32\%$ in male and $24\~20$ years accounted for $46.77\%$ in female of the total physical therapiests are the highest. 4. In job satisfaction, $44.5\%$ of physical therapiests who work in general hospital are generally satisfied and $25\%$ are 5. The periods of clinical practice for which physical therapiests expect to devote are 164$(44,81\%)$ consider less than 10years, and $67(18.31\%)$ expect 20years. 6. The problems of physical therapy system in korea are deviated administration of a cantral executive committee$(39.94\%)$ and excessive production of resistered physical therapiests.

• 한국식물병리학회:학술대회논문집
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• 한국식물병리학회 2003년도 정기총회 및 추계학술발표회
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• pp.85.1-85
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• 2003

In most ascomycetes, a single mating type locus, MAT, with two alternate forms (MAT1-1 and MAT1-2) called idiomorphs, controls mating ability. In heterothallic ascomycetes these alternate idiomorphs reside in different nuclei. In contrast, most homothallic ascomycetes carry both MAT1-1 and MAT1-2 in a single nucleus, usually closely linked. An example of the latter is Gibberella zeae, a producer of mycotoxins such as trichothecene and zearalenone that threaten human and animal health. We asked if G. zeae could be made strictly heterothallic by manipulation of MAT. Targeted gene replacement was used to differentially delete MAT1-1 or MAT1-2 from a wild type haploid MAT1-1 MAT1-2 strain, resulting in MAT1-1；mat1-2, mat1-1；MAT1-2 strains that were self-sterile, yet able to cross to wild type testers and more importantly, to each other. These results indicated that differential deletion of MAT idiomorphs eliminates selfing ability of G. zeae, but the ability to outcross is retained. To our knowledge, this is the first report of complete conversion of fungal reproductive strategy from homothallic to heterothallic by targeted manipulation of MAT. Practically, this approach opens the door to simple and efficient procedures for obtaining sexual recombinants of G. zeae that will be useful for genetic analyses of mycotoxin production and other traits, such as ability to cause disease.

• Asian-Australasian Journal of Animal Sciences
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• 제28권12호
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• pp.1721-1728
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• 2015

Embryonic stem cells (ESCs) have been used as a powerful tool for research including gene manipulated animal models and the study of developmental gene regulation. Among the critical regulatory factors that maintain the pluripotency and self-renewal of undifferentiated ESCs, NANOG plays a very important role. Nevertheless, because pluripotency maintaining factors and specific markers for livestock ESCs have not yet been probed, few studies of the NANOG gene from domestic animals including bovine have been reported. Therefore, we chose mouse ESCs in order to understand and compare NANOG expression between bovine, human, and mouse during ESCs differentiation. We cloned a 600 bp (-420/+181) bovine NANOG 5'-flanking region, and tagged it with humanized recombinant green fluorescent protein (hrGFP) as a tracing reporter. Very high GFP expression for bovine NANOG promoter was observed in the mouse ESC line. GFP expression was monitored upon ESC differentiation and was gradually reduced along with differentiation toward neurons and adipocyte cells. Activity of bovine NANOG (-420/+181) promoter was compared with already known mouse and human NANOG promoters in mouse ESC and they were likely to show a similar pattern of regulation. In conclusion, bovine NANOG 5-flanking region functions in mouse ES cells and has characteristics similar to those of mouse and human. These results suggest that bovine gene function studied in mouse ES cells should be evaluated and extrapolated for application to characterization of bovine ES cells.

• 한국유기농업학회지
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• 제24권4호
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• pp.665-680
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• 2016

Theoretically, it is said that economies of scope can be realized in the cropelivestock cycling organic farming. Thus, it is also used as the principle of organic farming. However, it is difficult to find the cases of the empirical analysis of it in Korea. In that sense, this study is meaningful in that it analyzed the agricultural data of case farms of obtaining the approval of both organic agricultural products and organic animal products and practicing cycling farming for 4 years and tested the hypothesis. This study measured economies of scope by using the actual measurement value and estimation value farming performance statistics for 4 years of case farms. This farmhouse conducted nutrient cycling in the farm like self-manufacturing and injecting organic agricultural byproduct and wild grass as organic livestock feed and fermenting organic livestock manure to organic compost to return it 100%. The results can be summarized as follows: According to the result of cycling farming of combining and producing organic agriculture and organic livestock, economies of scope were found to be realized in this case farmhouse. That is, although not strong, EOS>0, there were economies of scope. The measurement value appeared as 0.0722, 0.00378, 0.04667 and 0.13127 in 2012, 2013, 2014 and 2015, respectively. It was improved as time passes and the scale gets smaller. Therefore, in order to further improve economies of scope, there should be measures of reducing duplication costs between agriculture-livestock as low as possible and lowering the production cost of organic feed. That is, there is a need for the management strategy to adjust the import function and cost function according to the change in management paradigm and cropping system.

• 동의생리병리학회지
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• 제17권6호
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• pp.1433-1440
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• 2003

This research was performed to investigate protective effect of Sophorae subprostratae Radix and each fractions against ischemic damage using PC12 cells. To observe the protective effect of Sophorae subprostratae Radix on ischemia damage, vibility and changes in activities of Superoxide dismutase (SOD), Glutathione Peroxidase (GPx), Catalase and Production of Malondialdehyde (MDA) were observed after treating PC12 cells with Sophorae subprostratae Radix during ischemic insult. Groups were divided into five groups: no treated (Normal), hypoxia chamber for 48hrs followed by 6h at normoxic chamber (H/R), Sop horae subprostratae Radix total phase treated group with H/R (Total), Sophorae subprostratae Radix water phase treated group with H/R (Water), Sophorae subprostratae Radix BuOH phase treated group with H/R (BuOH), Sophorae subprostratae Radix alkaloid phase treated group with H/R (Alkaloid). The results showed that (1) in hypoxiajreperfusion model using PC12 cell, the Sophorae subprostratae Radix has the protective effect against ischemia in the dose of 0.2 ㎍/㎖, 2 ㎍/㎖ and 20 ㎍/㎖, (2) Sophorae subprostratae Radix increased the activities of glutathione peroxidase and catalase. (3) the activity of Superoxide Diamutase(SOD) increased by ischemic damage, which might represent the self protection. This study suggests that Sophorae subprostratae Radix has neuroprotective effect against neuronal damage following hypoxiajreperfusion cell culture model using PC12 cell and dose dependency effects. In conclusion, Sophorae subprostratae Radix has protective effects against ischemic oxidative damage at the early stage of ischemia.

• 지역사회간호학회지
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• 제11권1호
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• pp.117-126
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• 2000

For basic data to develop health management program of female workers. this study was intended to characterize factors affecting health problems of women at workplace which has less than 50 workers by Cornell Medical Index(CMI). 195 items of CMI questionnaire were simplified to 57 items which were composed of 35 items concerning physical health problems and 22 items concerning mental health problems. The data was collected during 6 months from July 10 to December 30 and analyzed with frequency, percentage, t-test and ANOVA. The 107 female workers included in this study was characterized to be mainly twenties(64.5%), single(69.2%) and working at production position(89.7%). The most frequent physical problem was fatigability and the next, neurological system and cardiovascular system. Main mental complaints were inadquacy. anger and tension. When the health problems were reviewed according to its general characteristics, anger in young age, adaptiveness in single and respiratory complaints in self-recognized unhealthy group were prevalent. According to life styles. the prevalent complaints were musculoskeletal one in alcoholic group and cardiovascular one in group who are sleeping just for four to five hours. The appropriateness of each items of CMI should be evaluated to reveal the reasons why meaningful results could not come from many items of this questionnaire. The repeated study should be carried out to figure out the health problems and its related factors of female workers at small scale workplace. Also, exercise program and abstinence from drinking and smoking should be carried out for female worker's health.

• 한국식생활문화학회지
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• 제12권1호
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• pp.87-96
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• 1997

The purpose of this study is, to examine current foodservice management practices at free meal service organization for elderly people and, to evaluate the attitude of recipients about the service and their ecological background. 6 meal service center as well as randomly selected 120 recipients at Sungnam area were surveyed and interviewed and result were summarized as follow. The cost of each meal (lunch) was ranged from 1,300 won to 1,500 won and number of attendant at meal service were ranged from 50 to 200 persons. Meal time for lunch begins from 10:30 am to noon because greater portion of people (elderly) didn't take breakfast frequently. Most of the center adapted self-service system. Standard recipe was not developed and meal preparation was controlled under the experiences of volunteer’s. Recording system of, nutrition management, production control, storage and inventory control was not well adapted by most of the center. In order to measure the level of storage, sanitation etc., scorin system in survey was adapted in this study and result are as follow: The score of sanitation of kitchen was lower than dinning area and that of food storage was lowest score. It was suggested that not only financial but also systematical support on management by local government may be necessary to meet the goal of supply nutritionally balanced food at the center. The score given by the recipient on the satisfaction of meal service was rate as 4.8 at the 5-point maximum scale. Meeting friends and share social relationship was major reason (41.6% of the total) of visiting to the center. It is suggested that in order to meet the changes of the patterns of change of social and family structure, the service of the center should be extended in urban area and it is necessary to develop systematic management models for the center.

• 한국보건간호학회지
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• 제14권1호
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• pp.147-160
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• 2000

This study was carried out to investigate awareness and attitude of monitors in 206 small sized industries in Seoul and Kyeonggi area toward the occupational health programs using a specially prepared questionnaire. The industries had been running Government-Funded Subsidiary Program since 1998. The questionnaire was designed to be self-administered by respondents. Data were collected for two months from November to December, 1998. Major results are as follows: 1. Between two groups of industries, there were no significant differences in classification, scale, methods of production, existence of trade union, but in type of working pattern and facility ownership there were significant differences. 2. Between two groups of monitors, there were no significant differences in gender, age, marital status, rank, years of education, but in duration of working there was a significant difference. 3. In awareness and attitude of monitors toward their activities and occupational health programs, there was no significant difference by groups of industries, but a significant difference by type and scale of industry, gender, age, years of education 4. It was found that newly subsidized industries were more necessary to provide education programs on occupational health than old industries, and there was a significant difference between two groups of industries in the contents of education needed. In conclusion, it is desirable that the government should develop specific training programs for monitors on occupational health to provide them practical knowledge and specific skills which monitors can apply to their specific situation, and implement the training programs and continue Government-Funded Subsidiary Program for years until small scale industries would have been able to develop and keep their own health management system spontaneously.

• 대한공업교육학회지
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• 제32권2호
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• pp.155-170
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• 2007

본 연구에서는 수리능력, 조직이해력, 기술능력, 문제해결력, 대인관계능력, 의사소통능력, 자기관리 및 개발능력, 자원활용능력, 정보활용능력을 직업기초능력의 영역으로 설정하여 자동차 분야 학생과 자동차 생산 관리 분야 산업체 인사의 직업기초능력 수준을 평가하였다. 각 영역의 직업기초능력의 수준을 설정하기 위해서는 자동차 분야 산업체와 대학의 직업기초능력의 수준을 설정할 수 있는 근거가 필요하므로 이를 위해 자동차 분야 산업체 인사가 보유하고 있는 직업기초능력의 수준을 조사하였다. 이를 토대로 자동차 분야의 2년제 대학 학생이 보유하고 있는 직업기초능력과의 차이를 분석하였다. 연구 결과 본 연구에서 정한 직업기초능력 수준 설정의 근거가 타당함을 검증하였고, 설정된 직업기초능력 수준을 적용시킬 때 자동차 분야 산업체와 대학 각각의 수준 평가가 가능하고 유효한 것임을 알 수 있었다.

• 대한한의학회지
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• 제24권1호
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• pp.29-40
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• 2003

Object : This research was performed to investigate the protective effect of Aurantii Immaturus Fructus against ischemic damage using PC12 cells and global ischemia in gerbils. Methods : To observe the protective effect of Aurantii Immaturus Fructus on ischemia damage, viability and changes in activities of superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase and production of malondialdehyde (MDA) were observed after treating PC12 cells with Aurantii Immaturus Fructus during ischemic insult. Gerbils were divided into three groups : a normal group, a 5-min two-vessel occlusion (2VO) group, and an Aurantii Immaturus Fructus administered after 2VO group. The CCAs were occluded by microclip for 5 minutes. Aurantii Immaturus Fructus was administered orally for 7 days after 2VO. The histological analysis was performed at 7 days after the surgery. For histological analysis, the brain tissue was stained with 1% cresyl violet solution. Results : The results showed that 1. Aurantii Immaturus Fructus had a protective effect against ischemia in the CAI area of the gerbil hippocampus 7 days after 5-minute occlusion, 2. In the hypoxia/reperfusion model using PC12 cells, the Aurantii Immaturus Fructus had a protective effect against ischemia in the dose of $0.2{\;}\mu\textrm{g}/ml,{\;}2{\;}\mu\textrm{g}/ml{\;}and{\;}20{\;}\mu\textrm{g}/ml$ 3. Aurantii Immaturus Fructus increased the activities of glutathione peroxidase and catalase, 4. The activity of superoxide dismutase (SOD) was increased by ischemic damage, which might represent self protection. This study suggests that Aurantii Immaturus Fructus has some neuroprotective effect against neuronal damage following cerebral ischemia in vivo with a widely used experimental model of cerebral ischemia in Mongolian gerbils, and it also has protective effects on a hypoxia/reperfusion cell culture model using PCq2 cells. Conclusions : Aurantii Immaturus Fructus has protective effects against ischemic brain damage at the early stage of ischemia.