• Korean Journal of Medicinal Crop Science
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• v.16 no.5
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• pp.287-293
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• 2008

This study was conducted to determine the characteristics of fertilization process and embryo development of Astragalus membranaceus Bunge (Astragali Radix) to provide basic data needed in its breeding. A. membranaceus showed poor seed setting when self-pollination was induced. When artificial pollination was induced, it showed less than 5% bearing in late August, but more than 13% bearing from the beginning of September 4th. The flower size was about $17.0\;mm{\times}4.0\;mm$ and pistils and stamens had the same length of 15.0mm at flowering stage. When self-pollination or cross-pollination was induced, pollen tubes extended to an ovule. While pollen tube was extending to the ovule, reproductive cell split and formed two male generative nuclei and a vegetative nucleus. In the case of self-pollination, fertilized embryo was not observed, but was formed in the case of cross-pollination. A. membranaceus is noted to have zygote self-incompatibility. In the case of cross-pollination, fertilization was observed in 6 to 8 h after pollination, where apical cell derivatives split after fertilization. A spherical pro-embryo was then formed three days after fertilization. The seed attained full shape with a seed coat showing its distinctive contour 15 days after fertilization. Thus, A. membranaceus in Leguminosae family is found to have zygote selfincompatibility although its flower shape is shown to match the self-compatibility plant.

• Journal of Korean Academy of Nursing
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• v.42 no.3
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• pp.369-376
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• 2012

Purpose: The purpose of this study was to identify the effects of a Yoga-focused prenatal program on the stress, anxiety, self confidence and labor pain of pregnant women who had in vitro fertilization (IVF) treatment. Methods: A quasi experimental study with a non-equivalent control group pretest-posttest design was used. The data collection period and meditation program were between January 9 and August 31, 2009. Forty-six women who were pregnant following IVF, and were between 12-20 weeks gestation, participated in the study (23 experimental group, 23 control group). Data were analyzed using Chisquare test, Mann-Whitney U Test, ANCOVA, and Cronbach's alpha coefficients with the SPSS 12.0 for Windows Program. Results: Although the sample size was limited, women who participated in the program showed statistically significant improvements in stress, anxiety, labor pain, and labor confidence for women pregnant after IVF. Conclusion: The result indicate that this 12-week Yoga-focused educational program can be utilized for women pregnant following IVF to reduce their stress, anxiety, and labor pain, and to increase delivery confidence. It is suggested that the Yoga-focused educational program be offered to every pregnant woman.

• Plant Resources
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• v.8 no.2
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• pp.87-90
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• 2005

In tobacco, in vitro pollination has been successfully applied to overcome interspecific incompatibility. The use of this technique will make it possible to introduce DNA into pollen tubes just before fertilization. In this study, we showed improvement of the efficiency of in vitro self-pollination and introduction of foreign genes into pollen tubes by the method of polycation. A plasmid harbouring the GUS gene was introduced into pollen grains and pollen tubes, which had incubated on pollen germination medium(PGM), by polyornithine method. Transient expression of the GUS in pollen grains and pollen tubes that were treated with 0, 2, 5 and $10{\mu}g/m\ell$ DNA was observed. In results, combination of the techniques of polyornithine and in vitro pollination was efficient new technique for genetic transformation through fertilization processes.

• Korean Journal of Soil Science and Fertilizer
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• v.50 no.3
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• pp.195-202
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• 2017

The volcanic ash soils from the Jeju province have low available phosphate because of the high phosphate fixing power of the soils. Phosphorus fertilizerwas dressed after cultivating green manure crops and before cultivating potatoes in order to investigate potato yields, the available phosphate contents, and phosphorus balance in the soils. Green manure crops cultivated in this study were hairy vetch alone and hairy vetch and rye. During potato cultivation, four treatments were conducted in the experiments; NF (non fertilizer), P fertilizer ($N-P-K=0-25-0kg\;10a^{-1}$), NK fertilizer ($N-P-K=18-0-19kg\;10a^{-1}$), and NPK fertilizer ($N-P-K=18-25-19kg\;10a^{-1}$). There were no differences in the yields of potato stem and tuber from the cultivation plots of different green manure crops. However, in the plots with single-sown hairy vetch and mixed-sown hairy vetch and rye, the yields of potato stem and tuber were higher in the P fertilizer plot than in NF plot. The yield of tuber among the treatments with mixed-sown hairy vetch and rye was the highest in the NPK fertilizer plot. The available phosphate content in soils initially increased with time in all plots, but began to decrease gradually after Oct. 18. The available phosphate contents were high in the plots for phosphate fertilization, and the difference in available phosphate content between non-phosphorus fertilizer plots and phosphorus fertilizer plots increased with time. In the single-sown hairy vetch and mixed-sown hairy vetch and rye plot, the phosphorus balances in NF plot and NK fertilizer plot were very low, while those in the P fertilizer plot and NPK fertilizer plot were high. In conclusion, available phosphate contents in soil and the potato yields were increased by phosphorus fertilization when potatoes planted after cultivating hairy vetch and rye together, compared to hairy vetch alone.

• Korean Journal of Breeding Science
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• v.43 no.5
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• pp.442-447
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• 2011

This study was carried out to assess the potential of hypocotyl explants for multiple-propagation by intensive sweetener variety with high yielding in stevia of self-fertilization. Agronomical character of self-fertilization shown a height range 72.0~120.7 cm, branch's 4.9~56.6 in stevia. Selection of high content of Rebaudioside A (RA) from segregating population in mixing variety have a variety, 'DA1' (55.2%) from HPLC analysis. On a developing of multiple-propagation in stevia, hypocotyl explants used to select for reasonable medium under the influence of each plant growth regulators concentrations. In a Mix treatment of plant growth regulators, as Murashige and Skoog medium with 1.5 mg/L BA and 0.5 mg/L Kinetin showed the most favorable. We got the multiple-propagation of 30 shoots from one inoculated hypocotyl explants as the result of these bases. Rooting induced superbly on MS medium with 0.1 mg/L IBA with inoculated shoots. All of the rooting plants were adapted to a normal seedling. This method of multi-propagation is used effectively in Rebaudioside A productivity elevation.

• Korean Journal of Plant Resources
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• v.18 no.2
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• pp.302-308
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• 2005

This study was carried out to obtain the basic informations on the characteristics of gametophytes formation and embryo developement in Dicentra spectabilis. Microspore mother cells developed from archesporial cells, start meiosis when flower bud length reaches around 1 mm, formed tetrahedral type tetrad. The 4 microspores were separated. They were developed to male gametophytes, respectively. Megaspore mother cells were observed when flower bud length was $4{\sim}5\;mm$. The developemental type of megaspore was polygonum and embryo sac was amphitropous. Three large and distinctive antipodals did not degenerated and remained after embryo sac was developed. When the male and female gametophytes was fully developed, the length of stamen and style was very similar or stamen was shorter about 0.5 mm than that of style. This result indicates that self-fertilization can be occurred in this species. After fertilization, developing zygotic embryos showed various stages of development from globular to cotyledonary embryos, and zygotic embryo in seed scattering time seemed to have an early cotyledonary stage.

• Journal of Korean Academy of Nursing
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• v.31 no.1
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• pp.55-67
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• 2001

A phenomenological study was conducted to investigate the experiences of mothers pregnant via in vitro fertilization (IVF). Sample of nine mothers participated. Ten theme clusters emerged when the formulated meanings were organized into categories. During the infertility period, the participants were subject to self- depreciation, envy, anxiety, and depression. It changed their priorities in life from a job-oriented life to one where having a baby was the most important thing. After trying numerous alternative therapies, IVF became their last hope in having a baby. Since the success rate for IVF is low (only 20-30%), the participants for the treatment were overwhelmed with uncertainty, and it led to further anxiety, depression and despair. Success of pregnancy gave them extreme satisfaction, but they became very cautious in their day-to-day life because of their fear of abortion and early delivery. Some were even worried about the side effect of IVF during the pregnancy. Finally, the delivery of the baby gave them relief from the obligation of having a baby. Women did not have any difficulties in rearing a "test tube baby" except in the case of twins. Most women had no ethical difficulty in having a baby by IVF. However they did not wish this information to be revealed to other people. They again turned to IVF to have a son(s) when the resultory child(s) was a daughter(s). This is because of the strong preference for sons in Korean society.

• Korean Journal of Plant Resources
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• v.25 no.2
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• pp.285-292
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• 2012

Self-incompatibility (SI) system is a genetic barrier that prevents self-fertilization and promotes cross-pollination among different S genotypes. In many of these species, SI is controlled by a single genetic locus known as S locus, which prevents the fertilization by pollen with same locus. S RNases are the products of the S-locus expressed in the stylar tissue of Fuji Apple with gametophytic self-incompatibility system. This study investigated the various types of chemicals in order to select more effective inhibitors and activators. The effect on the inhibition of S RNase of Fuji apples was investigated $in$ $vitro$. The result showed that the enzyme activity was reduced 24.3% by Iron(II) Sulfate, significantly. $In$ $vitro$ studies of pollen growth tube showed that pollen tube growth had a higher germination rate (90%) in 10% Sucrose than in 2% sucrose extension medium. Data on the fruit set of apples treated with inhibitor and activator. Double application of $A^+$(Apple Plus, ISTECH Co. Ltd.,)+Vitamin B6 had the highest central fruit set as 86.1%(Andong). One time application of $A^{++}$Vitamin B1 in Yeongju obtained the highest central fruit set (91.9%).

• KOREAN JOURNAL OF CROP SCIENCE
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• v.51 no.spc1
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• pp.264-268
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• 2006

This study investigated the effect of self-incompatibility control substance 'Apple plus' in improving self-fertilization and quality on apple. Results showed that the rate of fruit setting of disc florets of Fuji apple in the open field increased from 17.2% in 2004 to 44.4% in 2005 for the treated plots and from 0.5% in 2004 to 3.0% in 2005 for the control. In the case of using net covering, the rate of fruit setting increased from 3.3% in 2004 to 5.2% in 2005 for the treated plots and from 0.4% in 2004 to 0.1% in 2005 for the control. For the quality of apple fruits, results showed that there was no significant difference on the fruit L/D and firmness for both control and treated plots. However, the number of seeds was affected by the different substances which produced the following average number of seeds: control, 0.9; IS-1, 1.2; IS-2, 1.2; IS-3, 1.5 and IS-4, 0.7. The SSR genetic analysis showed that the rate of self-fertilization was highest for IS-1 (100%), followed by IS-2 (73%), IS-3 (68%), IS-4 (62%) and control (0%).

• Korean Journal of Plant Resources
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• v.33 no.6
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• pp.682-688
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• 2020

Hemp (Cannabis sativa L.) is a dioecious plant, although monoecious plants are bred in some cultivars for fiber or seed production. Recently, hemp has received attention for medicinal use with some cannabinoids, including cannabidiol. Self-fertilization for breeding inbred lines is difficult because of dioeciousness and anemophily in hemp. This experiment was conducted to develop a self-fertilization method by forming female flowers and seeds from male plants of dioecious hemp. To induce the formation of female flowers on male plants, 500 mg L^-1 of ethephon was sprayed on plants at soon, seven and fourteen days after primordia formation. The plant ratio of female flowers formation and the number of harvested seeds were increased by ethephon treatment. Female flowers of male plants have 5 stigmas in contrast to the dual stigma of female 1plants. Male plant seeds were lighter and smaller than those from female plants. Although the germination rate was lower than that of normal seeds from female plants, the seeds from male plants germinated to grow seedlings. Thus, we suggest that hemp plants should be treated with ethephon at soon after primordia formation to induce the formation of more female flowers on the male plants.