• Title/Summary/Keyword: Self-discrepancy

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Stabilization of Dry Immuno-Diagnostic Kit with Immobilized Antibody and Determination of Its Shelf-Life (항체가 건조상태로 고정된 면역진단키트의 안정화와 Shelf-Life 결정)

  • 이창우;조정환;육순학;권오협;박영남;박세환
    • KSBB Journal
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    • v.13 no.5
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    • pp.502-510
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    • 1998
  • Stabilization of antibody, which is specific to Salmonella typhimurium antigens, present in dry states on membranes was accomplished, and its shelf-life, i.e., duration for maintaining minimum 90% of the initial activity, under optimal conditions was determined. To prepare two major components of an immuno-strip, the antibody was not only immobilized on nitrocellulose membrane surfaces but also placed within the pores of glass fiber membrane after conjugating it with old colloids as signal generator. Among potential stabilizers of the immuno-components, a disaccharide, trehalose, showed a significant protection effect of immunoglobulin structure from thermal energy. Optimal concentrations of trehalose for the respective component were significantly different (8-fold higher for the antibody-gold conjugate than for the immobilized antibody), which probably resulted from distinct densities and configurations of antibody present on the membranes. An additional requirement for the gold conjugate was freeze-drying of this substance such that the conjugate can be readily resolubilized upon contact with aqueous medium. By using the components prepared under optimal conditions, immuno-strips were constructed and exposed to thermal energy. Signals with less than 10% decrease in the intensity were maintained for approximately 21 days at 60$^{\circ}C$. Compared to previous reports, this result represented a 2-year shelf-life at room temperature. it was, however, two times longer if determined from thermal acceleration tests based on the theory of inactivation rate of protein. Such discrepancy between the two estimates could be mainly attributed to errors in accurately controlling temperatures and also to changes in the physical properties of membranes due to a high thermal energy.

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A Study on The Grotesque Make-up in the Late 1990′s - Focused on Make-up Advertisements and Collections - (1990년대 후반 그로테스크 메이크업에 관한 연구 - 메이크업 광고와 컬렉션을 중심으로 -)

  • 장미숙;양숙희
    • The Research Journal of the Costume Culture
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    • v.8 no.3
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    • pp.436-499
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    • 2000
  • The purpose of this study is to be inquired social and cultural significance of the Grotesque phenomenon in the late 1990's make-up, as well as its experimentalism and avantgardism. The Grotesque is the aesthetic concept, which is composed of ambivalent structure of humor and horror, interest and disgust through join of heterogenous factors, distortion, extreme and overstatement in the patterns and materials of make-up. The Grotesque in the modern make-up is represented in the make-up advertisements and collections. In this paper, the author classified the Grotesque phenomenon with the Kitsch, the Machine, the Animal and the Devil, focused on heterogenous factors. The results are as follows. 1. The Kitsch in the make-up uses not cosmetics but imitations, ready-made goods, and ordinary materials. It expresses modern culture's laziness through shocking self-expression. 2. The Machine in the make-up means silver or white color's make-up, and represents cyber period's arrival through contrary irony, loss of the human value and the personifications of machine. At the same time, it is understood as the curing of human alinetion. 3. The Animal in the make-up utilizes the wild animal's horn, the reptiles'sputum, and the legend's animal. It points out a human false virtual image, and overturns make-up's traditional aesthetic concept. 4. The Devil in the make-up shows vampire's cuspid, demon's horn, artificial nail, and black color's make-up. It raises social irrationality and discrepancy of capitalism, and expresses paradoxically the nature of human life. The Devil in the make-up expresses the creator's innovation, anxiety of fin-de-siecle, and simultaneously criticizes and cures confused reality. It shows also avantgarde implementation of dissolution of traditional aesthetic concept and acceptance ugliness.

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The Cause and Economic Effects of Overeducation (과잉교육의 원인과 경제적 효과)

  • Oh, Ho young
    • Journal of Labour Economics
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    • v.28 no.3
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    • pp.1-37
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    • 2005
  • This article examines the reasons for the observed discrepancy between worker's actual and required levels of schooling and the resulting economic effects in wage and labor mobility using KRIVET's Graduates Economic Activities Survey in 2005. Overeducated workers in Korea are estimated to be 10.1%-20.7% among junior college graduates and 18.8% among university graduates for their first job according to job analysis method and worker's self-assessment method each. The effects of school quality-measured by the student/teacher ratio, school building area/student ratio-on the overeducation of their graduates are found to be mostly insignificant not only junior colleges but also universities. This founding implies that the increase of education investment from schools does not acquire any recognition from the labor market. Also, the effects of overeducation and school quality on wages are found to be insignificant and job mobility has little effects on improving overeducated workers' job match.

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Evaluation of dental web site usability in Korea (국내 치과 웹사이트에 대한 사용성 평가)

  • Kim, Seon-Yeong;Kim, Yun-Jeong
    • Journal of Korean society of Dental Hygiene
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    • v.16 no.2
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    • pp.241-248
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    • 2016
  • Objectives: The purpose of the study is to evaluate the dental website usability in Korea. The dental website included dental university hospital, network dental hospital and dental office. Methods: The study was carried out by three age groups including twenties, thirties, and forties. Each group consisted of ten customers and evaluated twelve websites of dental hospitals. Each was assigned to four hospitals and three tasks including easiness of online reservation, preventive information, and treatment information. They filled out the self-administered questionnaire. The questionnaire included the easiness of on-line reservation, satisfaction on the quality and quantity of preventive informations, satisfaction on the quality and quantity of treatment informations, predictiveness on the sub-menu, usefulness of site map, information on the main page of web site, usefulness of decision on visit to dentistry, and revisit intention. Results: The easiness of on-line reservation was the highest in the private dental office, and university dental hospital and network dental hospitals followed in conducting task(1). The anticipated value and measurement on the usefulness of web site were the lowest in network dental hospitals and the time interval between two values was 57 seconds. This discrepancy showed the largest difference. The satisfaction on treatment information in task(3) was higher than that of the satisfaction on preventive information in task (2). The revisit intention was the highest in dental university hospitals. Conclusions: This study showed the comparison in usefulness of web site of university dental hospitals, network dental hospitals and private dental office. The web site focused on the treatment information rather than preventive information. This study suggested that the most important function of dental web site would be the preventive information that was mainly operated by the role of dental hygienists rather than treatment information in the future.

Anger Assessment Using State-Trait Anger Expression Inventory in Middle-School Students in Korea and Association with Depression (한국판 상태-특성 분노표현척도를 이용한 한국 청소년의 분노 평가 및 우울과의 관계)

  • Kim, Hee-Yun;Lee, Myung-Hoon;Bae, Jae-Nam;Kim, Chul-Eung;Yoo, Hee Jeong;Lee, Jeong-Seop
    • Journal of the Korean Academy of Child and Adolescent Psychiatry
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    • v.26 no.4
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    • pp.288-294
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    • 2015
  • Objectives : The purpose of this study was to assess anger of middle school adolescents in an urban community using State-Trait Anger Expression Inventory (STAXI)-Korean and Korean Child Behavior Checklist (K-CBCL), and to determine whether specific anger expression of adolescents is associated with certain psychopathology including depression. Methods : Data were collected from 395 middle school students and their parents in Incheon city. The students completed the STAXI, Children's Depression Inventory, Korean version of Mood disorder Questionnaire, and Conners-Wells' Adolescent Self-Report Scale (Short Form), and their parents completed the K-CBCL. Results : No significant correlation was observed between aggression subscale K-CBCL and each scale of the STAXI, except anger out (r=0.704). The scores for STAXI total and respective scales were significantly higher for female students than male students. A stepwise logistic regression model was used to explore the possible predictors for depression in adolescents and lower anger control and higher anger suppression were found to be predictors for depression in adolescence. Conclusion : There is a possibility of a large discrepancy between anger that the adolescents actually feel and parents' judgment of their children's anger. It must be considered in assessment of adolescent anger and use of further structured interviews is necessary. In addition, it may be useful to consider the anger expression style in adolescents who report depressive symptoms.

Workplace Situation and Satisfaction Level of Dental Laboratory Technicians (치과기공사의 직업만족도에 대한 조사 연구 I (근무처에 대한 만족도))

  • Oh, Sae-Yoon;Kim, Ji-Hwan;Kim, Woong-Chul;Yu, Chin-Ho;Kim, Tae-Suk;Lee, Tae-Jung
    • Journal of Technologic Dentistry
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    • v.28 no.1
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    • pp.143-159
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    • 2006
  • Objectives; The purpose of the present study is to investigate workplace satisfaction level of dental technicians and the differences between that of dental lab owner's and that of employed dental technicians, who are working in dental laboratories. Methods; Samples were comprised of 137 dental technicians working in dental laboratories. Data were collected through self-administered questionnaires and analyzed to frequency distribution, correlation, t-test and multiple regression analysis. Results; 1) Dental technicians generally are 'somewhat or a bit satisfied' with their workplace situations, regardless of position, sex, and individual career. 2) Mean values of 4 items out of 11 work situation factors of employed dental technicians were significantly lower than those of lab owner's. The 4 items are the openness of management, working environment, pay, and policy in promotion and raise. They would be sources of dissatisfaction and conflict. 3) Level of satisfaction was significantly correlated with 2 subordinate variables, i.e. working conditions and administration policy. There is a tendency that administration policy is a bit more important for lab owners, while working conditions are a bit more important for employed dental technicians. Conclusion; The employer-employee relationship of dental technicians seems to be sound, as no signs of troublesome problem is detected. But the discrepancy revealed in the above results is telling that there certainly exist some disagreement in the viewpoints of the two groups. It is necessary for the dental technicians to find ways to deal with the possible triggers of discord and discontent.

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Performance of foreign patients attract according to the type, geographical position and scale of hospital (병원의 유형, 지리적 위치, 규모에 따른 외국인환자 유치 성과 연구)

  • Park, Cho-Hee;Ahn, Sang-Yoon
    • Korea Journal of Hospital Management
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    • v.20 no.3
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    • pp.13-23
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    • 2015
  • This research is intended to inquire any discrepancy depending on the variables such as types, geography and size of hospitals of Korea in order to develop differentiated marketing strategy and to investigate how the aforementioned variables affect the management performance such as the increase in the number of foreign patients, their willingness to re-visit, the increase in profit and etc. The survey for this research was conducted for five weeks from July 10th 2014 to August 30th 2014 with 251 participants in charge of foreign patient attraction for 161 domestic hospitals. As the method of the research, a hypothesis was first established based on previous studies, followed by the incorporation of self-administered questionnaire to confirm the formulated hypothesis. Frequency analysis and ANOVA analysis were used to analyze the result of the survey. The outcome of the research and the implications are as follows. First, according to the demography of the persons-in-charge of foreign patient attraction, the proportion of female was superbly high by 76.5%. As for the age, those in their 30s were highest in proportion. Finally, for the education level, college graduates took up the largest portion by 46.5%. Second, in respect to the hypothesis assuming the difference in performance depending on the types of hospitals, national university-affiliated hospitals showed the highest level of the increase in foreign patients by the average of 3.25. Third, in respect to the hypothesis assuming the difference in performance based on geographical position, it was confirmed that hospitals in Ulsan City experienced the largest growth in the number in foreign patients. Fourth, in respect to the sizes of hospitals, those with 201 to 300 sickbeds showed the highest increase by the average of 3.45. The implication of the above research outcome indicates that while the number of foreign patients visiting Korea for medical purposes is on the rise, the number of professionals in place with necessary knowledge and capacity is insufficient and requires improvement.

An Analysis on Clinical Education of Pediatric Nursing (아동간호학 임상실습교육 현황)

  • Kwon In-Soo
    • Child Health Nursing Research
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    • v.8 no.3
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    • pp.344-356
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    • 2002
  • This study was conducted to analyse the current clinical education of pediatric nursing in baccalaurate nursing program, then to give basic data for enhancing the quality of future clinical education of pediatric nursing. Data were collected through self-reported questionnaire by mail from December 2001 to February 2002. The subjects were 29 schools of 50 baccalaurate nursing education programs. The data were analysed by double raters, researcher and assistant researcher. The results were summarized as follows: 1. Twenty-eight schools had the objectives of the clinical education of pediatric nursing, and 28 schools in pediatric ward, 23 schools in nursery, 22 schools in neonatal intensive care unit(NICU), 15 schools in objectives related to profession by clinical site. 2. Credits on clinical education of pediatric nursing were most 15 schools of 3 credits. 3. The clinical sites were mainly the hospital that sick children were admitted in. 4. The clinical teacher were 9 types including pediatric professor and field nurse. 5. On teacher's role, the professor instructed the case study and conference, and field nurse instructed the patient assignment and nursing procedures. 6. All of schools used explanation and conference as a method of clinical education, 1 or 2 schools used PBL or role play or field study. 7. On clinical education content, most of school included Apgar scoring system, physical examination in newborn assessment, respira- tion maintenance, temperature maintenance, infection prevention, nutrition, and bath in newborn care. 8. On clinical education content, most of school included care of incubator, phototheraty, infusion, gavage feeding and how to use the instruments in NICU. Eighteen schools included attachment promotion, and 20 schools case study. 9. On clinical education content, most of school included a checklist of nursing procedures, case study, assessment of growth and development in pediatric ward and other sites. 10.There were various evaluation types in scores, measuring items. In conclusion, the results of this study revealed that there were some discrepancy in the objectives and contents, clinical sites on hospital focused, teacher's role, and diversity of measurement items and ratings in clinical education of pediatric nursing. There is a need for a standardization of content, clinical site, and evaluation tool to improve a quality of clinical education of pediatric nursing based on this study.

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Cyclic Alternating Pattern : Implications for Insomnia (불면증에서 순환교대파형의 의미)

  • Cyn, Jae-Gong
    • Sleep Medicine and Psychophysiology
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    • v.17 no.2
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    • pp.75-84
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    • 2010
  • The cyclic alternating pattern (CAP) is a periodic EEG activity in NREM sleep, characterized by sequences of transient electrocortical events that are distinct from background EEG activities. A CAP cycle consists of two periodic EEG features, phase A and subsequent phase B whose durations are 2-60 s. At least two consecutive CAP cycles are required to define a CAP sequence. The CAP phase A is a phasic EEG event, such as delta bursts, vertex sharp transients, K-complex sequences, polyphasic bursts, K-alpha, intermittent alpha, and arousals. Phase B is repetitive periods of background EEG activity. The absence of CAP more than 60 seconds or an isolated phase A is classified as non-CAP. Phase A activities can be classified into three subtypes (A1, A2, and A3), based on the amounts of high-voltage slow waves (EEG synchrony) and low-amplitude fast rhythms (EEG desynchrony). CAP rate, the percentage of CAP durations in NREM sleep is considered to be a physiologic marker of the NREM sleep instability. In insomnia, the frequent discrepancy between self-reports and polysomnographic findings could be attributed to subtle abnormalities in the sleep tracing, which are overlooked by the conventional scoring methods. The conventional scoring scheme has superiority in analysis of macrostructure of sleep but shows limited power in finding arousals and transient EEG events that are major component of microstructure of sleep. But, it has recently been found that a significant correlation exists between CAP rate and the subjective estimates of the sleep quality in insomniacs and sleep-improving treatments often reduce the amount of CAP. Thus, the extension of conventional sleep measures with the new CAP variables, which appear to be the more sensitive to sleep disturbance, may improve our knowledge on the diagnosis and management of insomnia.

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Assessment of Readability and Appropriate Usability Based on the Product Labelling of Over-The-Counter Drugs in Korea (일반의약품 설명서의 이해도와 적정 사용가능성 평가)

  • Lee, Iyn-Hyang;Lee, Hyung Won;Je, Nam Kyung;Lee, Sukhyang
    • YAKHAK HOEJI
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    • v.56 no.5
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    • pp.333-345
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    • 2012
  • A product labelling is one of key tools in ensuring that a patient uses drugs safely and effectively in self-care without professional support. This study aimed to explore the readability and comprehensibility of the information contained on two package inserts of medicines. Two package inserts were tested with first year college students. Fifty-one potential consumers underlined words they could not understand and answered 10 scenario questions. Any differences among groups with different characteristics were statistically tested. Secondly, the readability of two package inserts was assessed with comparison to the level of the 6th grade Korean textbooks. As results, more than 80% of participants properly replied to straightforward questions concerning indication, dosage, duplication, use in pregnancy and contraindication, and 73% about formulation. Less than half answered correctly in multiple choice questions about pediatric use (41%) and side effects (35%). Little discrepancy was observed in the comprehensibility between participants' characteristics. Drug inserts contained about 20% more professional-level words than 6th grade textbooks. In conclusion, Korean consumers may face challenges to understand drug information due to professional terminology and outdated expressions in the current package inserts. To secure safe and effective use of over-the-counter agents, greater efforts should be made to develop more consumer friendly labels. In the other hand, educational supports are required to prepare consumers in a proper level of knowledge for the safe use of drugs.