• KIPS Transactions on Computer and Communication Systems
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• v.2 no.8
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• pp.335-342
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• 2013

Nowadays, the traffic type and behavior are extremely diverse due to the growth of network speed and the appearance of various services on Internet. For efficient network operation and management, the importance of application-level traffic identification is more and more increasing in the area of traffic analysis. In recent years traffic identification methodology using statistical features of traffic flow has been broadly studied. However, there are several problems to be considered in the identification methodology base on statistical features of flow to improve the analysis accuracy. In this paper, we recognize these problems by analyzing the ground-truth traffic and propose the solution of these problems. The four problems considered in this paper are the distance measurement of features, the selection of the representative value of features, the abnormal behavior of TCP sessions, and the weight assignment to the feature. The proposed solutions were verified by showing the performance improvement through experiments in campus network.

• Journal of the Korea Institute of Information Security & Cryptology
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• v.32 no.3
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• pp.555-564
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• 2022

Recently, cyberattacks are using hacking techniques utilizing intelligent and advanced malicious codes for non-face-to-face environments such as telecommuting, telemedicine, and automatic industrial facilities, and the damage is increasing. Traditional information protection systems, such as anti-virus, are a method of detecting known malicious URLs based on signature patterns, so unknown malicious URLs cannot be detected. In addition, the conventional static analysis-based malicious URL detection method is vulnerable to dynamic loading and cryptographic attacks. This study proposes a technique for efficiently detecting malicious URLs by dynamically learning malicious URL data. In the proposed detection technique, malicious codes are classified using machine learning-based feature selection algorithms, and the accuracy is improved by removing obfuscation elements after preprocessing using Weighted Euclidean Distance(WED). According to the experimental results, the proposed machine learning-based malicious URL detection technique shows an accuracy of 89.17%, which is improved by 2.82% compared to the conventional method.

• Asian-Australasian Journal of Animal Sciences
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• v.31 no.11
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• pp.1691-1699
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• 2018

Objective: In Korea, there are three main cattle breeds, which are distinguished by coat color: Brown Hanwoo (BH), Brindle Hanwoo (BRH), and Jeju Black (JB). In this study, we sought to compare the genetic diversity and divergence among there Korean cattle breeds using a BovineHD chip genotyping array. Methods: Sample data were collected from 168 cattle in three populations of BH (48 cattle), BRH (96 cattle), and JB (24 cattle). The single-nucleotide polymorphism (SNP) genotyping was performed using the Illumina BovineHD SNP 777K Bead chip. Results: Heterozygosity, used as a measure of within-breed genetic diversity, was higher in BH (0.293) and BRH (0.296) than in JB (0.266). Linkage disequilibrium decay was more rapid in BH and BRH than in JB, reaching an average $r^2$ value of 0.2 before 26 kb in BH and BRH, whereas the corresponding value was reached before 32 kb in JB. Intra-population, interpopulation, and Fst analyses were used to identify candidate signatures of positive selection in the genome of a domestic Korean cattle population and 48, 11, and 11 loci were detected in the genomic region of the BRH breed, respectively. A Neighbor-Joining phylogenetic tree showed two main groups: a group comprising BH and BRH on one side and a group containing JB on the other. The runs of homozygosity analysis between Korean breeds indicated that the BRH and JB breeds have high inbreeding within breeds compared with BH. An analysis of differentiation based on a high-density SNP chip showed differences between Korean cattle breeds and the closeness of breeds corresponding to the geographic regions where they are evolving. Conclusion: Our results indicate that although the Korean cattle breeds have common features, they also show reliable breed diversity.

• Journal of Animal Science and Technology
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• v.46 no.2
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• pp.155-164
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• 2004

This involves identifying and cloning trapped genes from cultured cells carrying the gene-trap constructs and generating cloned zebrafish using these cells for functional study. Gene-trapping studies in gene-trapped cells were carried out in initial and cloned zebrafish carrying gene-trap events were successfully produced based on the nuclear transplantation technique. Two kind of retroviral gene-trap constructs were adopted. The first one(SA/GFP-TP), constructed in my laboratory, carries a GFP reporter gene containing a splicing acceptor and an internal neo gene. The second one(Neo-TP), obtained from Dr. Hicks (Hicks et al., 1997), contains a promoter-less neo gene located in the LTR sequence of a retroviral vector. The infected cells were subjected to drug selection(neomycin treatment) because the two constructs carry the neomycin resistant gene. All those cells survived the neomycin treatment should carry the proviral insertions. For Neo-TP, Isolated DNA from the neomycin-resistant fibroblast cells infected by Neo-TP, was digested with EcoR1 restriction enzyme and transformed into bacteria after ligation. This procedure led to the isolation of seven clones carrying flanking cellular DNA with a typical retroviral integration signature sequence. These clones contained genomic DNA ranging from 1kb to 7kb and sequences of 300-600 bp were obtained from each of the rescued plasmids. Database searching showed that all of them share high homology to zebrafish sequences. For fish cloning using tagged cells, initially, nucleus donors directly selected from a mixture of cells(Neo-TP cells) were used. A total of 44 embryos(3.7%) out of 1179 transplants were reached blastula stage; 8 of these embryos(0.8%) hatched and 3(0.3%) of them survived to adulthood. One out of three lived cloned zebrafish has an amplified fragment and was labeled with 32P.

• Journal of the Korea Institute of Information Security & Cryptology
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• v.33 no.3
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• pp.499-510
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• 2023

Recently, an intelligent and advanced cyber attack attacks a computer network of a public institution using a file containing malicious code or leaks information, and the damage is increasing. Even in public institutions with various information protection systems, known attacks can be detected, but unknown dynamic and encryption attacks can be detected when existing signature-based or static analysis-based malware and ransomware file detection methods are used. vulnerable to The detection method proposed in this study extracts the detection result data of the system that can detect malicious code and ransomware among the information protection systems actually used by public institutions, derives various attributes by combining them, and uses a machine learning classification algorithm. Results are derived through experiments on how the derived properties are classified and which properties have a significant effect on the classification result and accuracy improvement. In the experimental results of this paper, although it is different for each algorithm when a specific attribute is included or not, the learning with a specific attribute shows an increase in accuracy, and later detects malicious code and ransomware files and abnormal behavior in the information protection system. It is expected that it can be used for property selection when creating algorithms.

• Journal of Life Science
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• v.25 no.3
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• pp.349-356
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• 2015

Thanks to recent advances in next-generation sequencing (NGS) technology, diverse livestock species have been dissected at the genome-wide sequence level. As for cattle, there are currently four Korean indigenous breeds registered with the Domestic Animal Diversity Information System of the Food and Agricultural Organization of the United Nations: Hanwoo, Chikso, Heugu, and Jeju Heugu. These native genetic resources were recently whole-genome resequenced using various NGS technologies, providing enormous single nucleotide polymorphism information across the genomes. The NGS application further provided biological such that Korean native cattle are genetically distant from some cattle breeds of European origins. In addition, the NGS technology was successfully applied to detect structural variations, particularly copy number variations that were usually difficult to identify at the genome-wide level with reasonable accuracy. Despite the success, those recent studies also showed an inherent limitation in sequencing only a representative individual of each breed. To elucidate the biological implications of the sequenced data, further confirmatory studies should be followed by sequencing or validating the population of each breed. Because NGS sequencing prices have consistently dropped, various population genomic theories can now be applied to the sequencing data obtained from the population of each breed of interest. There are still few such population studies available for the Korean native cattle breeds, but this situation will soon be improved with the recent initiative for NGS sequencing of diverse native livestock resources, including the Korean native cattle breeds.