• Title/Summary/Keyword: Selection Analysis

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Development and Verification of and Single Nucleotide Polymorphism Markers toDetermine Country of Origin of Korean and Chinese Scapharca subcrenata (한국산과 중국산 새꼬막(Scapharca subcrenata)의 원산지 판별을 위한 SNP 마커의 개발 및 검증)

  • Seong Seok Choi;Seung Hyun Yoo;Yong Bae Seo;Jong Oh Kim;Ik Jung Kwon;So Hee Bae;Gun Do Kim
    • Journal of Life Science
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    • v.33 no.12
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    • pp.1025-1035
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    • 2023
  • In this study, we analyzed SNPs that appear between Korean and Chinese Scapharca subcrenata using the nucleotide sequence data of S. subcrenata analyzed by genotyping by sequencing (GBS). To distinguish the country of origin for S. subcrenata in Korean and Chinese, we developed a primer set as single nucleotide polymorphism (SNP) markers for quantitative real-time PCR (qPCR) analysis and validated by sequencing SNPs. A total of 180 samples of S. subcrenata were analyzed by genotyping by sequencing, and 15 candidate SNPs were selected. SNP marker selection for country of origin were identified through real-time qPCR. Insertion 1 and SNP 21 markers showed the most distinct separation between the sequence types as well as the country of origin through qPCR, with the observed amplification patterns matching the expected outcomes.. Additionally, in a blind test conducted by mixing samples of S. subcrenata at random, Insertion 1 showed 74% accuracy, 52% sensitivity, and 96% specificity, and SNP 21 showed 86% accuracy, 79% sensitivity, and 93% specificity. Therefore, the two SNP markers developed are expected to be useful in verifying the authenticity of the country of origin of S. subcrenata when used independently or in combination.

Comparison Analysis on Characteristics and Components of Various Waste Plastic Pyrolysis Oils by Vacuum Distillation Method (감압증류를 통해 분획된 폐플라스틱 열분해유의 특성평가와 구성성분 비교분석 연구)

  • Hwayeon Jeon;Cheol Hwan Jeon;Sung Joon Park;Jae Woo Lee;Jae-kon Kim
    • Clean Technology
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    • v.29 no.4
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    • pp.262-271
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    • 2023
  • Globally, the amount of waste plastics has been occurring to environmental problems. As a result, it is necessary to research methods that utilize waste plastic pyrolysis oil (WPPO) produced by pyrolysis. One such method being studied is utilizing WPPO as a naphtha feedstock. In this study, five types of WPPO were analyzed to determine whether they can be used as raw materials for naphtha. Because of their wide boiling point range, the WPPOs were fractionated into light and heavy fractions through vacuum distillation, and the separation and purification techniques were analyzed using GC-VUV to determine the content of paraffin, olefin, and other compounds. All WPPOs showed high olefin content regardless of the source and fraction. Aromatic and paraffin content varied depending on the source, and oxygen and other compounds also varied significantly by source and fraction. In addition, the light fraction showed a carbon distribution similar to that of naphtha, whereas the heavy fraction showed a carbon distribution of C11 ~ C14. In conclusion, additional processes and raw material selection are required to utilize waste plastic pyrolysis oil as a raw material for naphtha.

Review on Rock-Mechanical Models and Numerical Analyses for the Evaluation on Mechanical Stability of Rockmass as a Natural Barriar (천연방벽 장기 안정성 평가를 위한 암반역학적 모델 고찰 및 수치해석 검토)

  • Myung Kyu Song;Tae Young Ko;Sean S. W., Lee;Kunchai Lee;Byungchan Kim;Jaehoon Jung;Yongjin Shin
    • Tunnel and Underground Space
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    • v.33 no.6
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    • pp.445-471
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    • 2023
  • Long-term safety over millennia is the top priority consideration in the construction of disposal sites. However, ensuring the mechanical stability of deep geological repositories for spent fuel, a.k.a. radwaste, disposal during construction and operation is also crucial for safe operation of the repository. Imposing restrictions or limitations on tunnel support and lining materials such as shotcrete, concrete, grouting, which might compromise the sealing performance of backfill and buffer materials which are essential elements for the long-term safety of disposal sites, presents a highly challenging task for rock engineers and tunnelling experts. In this study, as part of an extensive exploration to aid in the proper selection of disposal sites, the anticipation of constructing a deep geological repository at a depth of 500 meters in an unknown state has been carried out. Through a review of 2D and 3D numerical analyses, the study aimed to explore the range of properties that ensure stability. Preliminary findings identified the potential range of rock properties that secure the stability of central and disposal tunnels, while the stability of the vertical tunnel network was confirmed through 3D analysis, outlining fundamental rock conditions necessary for the construction of disposal sites.

Analysis of Ecological Space Connectivity and Forest axis in Daegu and Gyeongsangbuk-do (대구·경북 생태공간 연결성 및 산림축 분석)

  • Jae-Gyu CHA
    • Journal of the Korean Association of Geographic Information Studies
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    • v.26 no.4
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    • pp.80-96
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    • 2023
  • The expansion of human activities and road development has led to the loss and fragmentation of ecological spaces, which is a negative factor for biodiversity. In particular, urban areas where land use and land cover have rapidly changed into urbanization zones are regions where ecological spaces are lost and isolated, making it difficult for wildlife to inhabit. Furthermore, the loss and fragmentation of ecological spaces due to urbanization can have a negative impact on ecosystem services. Therefore, to enhance biodiversity and ecosystem services in urban and national land, it is necessary to establish a practical ecological axis that reflects the current status of the city. Thus, this study analyzed the connectivity of ecological spaces and forest axis that can be used for spatial planning related to urban ecological axis of local governments in Daegu and Gyeongsangbuk-do. The ecological connectivity was analyzed by dividing the Daegu-Gyeongbuk region into 31 local government units, distinguishing between forests and natural areas using land cover data. Subsequently, the study area was divided into 20,483 hexagonal grids of 1 square kilometer each, and the restoration effects for ecological fragmentation within 100 meters were spatially clustered to visualize priority restoration areas. The forest axis was derived by considering regional conditions such as land cover, building area, slope, and others to connect 1,534 forests of 100 hectares or more. The research results are expected to be used as fundamental data for spatial planning, goal setting, and the selection of restoration areas for improving ecological connectivity.

A Qualitative Research on the Evaluation of Healthcare and Welfare Network for Vulnerable Populations : Focusing on the Dalgubeol Health Doctor Services (취약계층 대상 보건의료·복지 네트워크 사업 성과에 대한 질적연구 : 달구벌건강주치의사업을 중심으로)

  • Su-Jin Lee;Jong-Yeon Kim;Jae-Wook Kang;Hye-Jin Lee
    • Journal of agricultural medicine and community health
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    • v.48 no.4
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    • pp.262-274
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    • 2023
  • Objectives: This study examined the evaluation and potential improvements of 'Integrated Healthcare and Social Welfare service model' based on the experiences of practitioners from institutions participating in the 'Dalgubeol Health Doctor Services' and the service recipients. Methods: Qualitative research was conducted from September to November 2022 in this study, focusing on 4 providers from the dedicated Dalgubeol Health Doctor Services Team, 5 contact partners from affiliated organizations, and 6 service beneficiaries. The data gathered underwent thematic analysis. Results: The evaluation indicated that Dalgubeol Health Doctor Services has proven to be effective in addressing the complex needs of vulnerable populations. By providing integrated services through quick and simple beneficiary selection and resource linkage, it has contributed to the resolution of complex demands, recovery of positive attitudes towards life, and improvement in quality of life for users who have fear the use of medical and welfare services. Dalgubeol Health Doctor Services has established an integrated health care system involving not only public but also private organizations, from the referral agency to the service provider. Centered around Daegu Medical Center and involving five tertiary hospitals, it has established a model that supports treatment appropriate to the severity of the patient, from mild to severe. Conclusions: These findings indicate an enhancement in health equity, achieved through the active identification and subsequent health and welfare issue resolution of individuals marginalized from medical benefits.

Physical habitat characteristics of freshwater crayfish Cambaroides similis (Koelbel, 1892) (Arthropoda, Decapoda) in South Korea

  • Jin-Young Kim;Yong Ju Kwon;Ye Ji Kim;Yeong-Deok Han;Jung Soo Han;Chae Hui An;Yong Su Park;Dongsoo Kong
    • Journal of Ecology and Environment
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    • v.47 no.4
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    • pp.200-210
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    • 2023
  • Background: Cambaroides similis is an endangered candidate species living in the stream of South Korea. Freshwater crayfish is known to decline rapidly not only domestically, but also internationally. Its decline is projected to be further exacerbated due to climate change. Understanding physical characteristics of the habitat is crucial for the conservation of an organism. However, comprehensive data regarding the distribution and physical habitat characteristics of C. similis are currently unavailable in South Korea. Thus, the objective of this study was to ascertain preferred ranges for water depth, current velocity, and streambed substrate of C. similis using Weibull model. Results: In this study, C. similis was found at 59 sites across 12 regions in South Korea. Its optimal water depth preferences ranged from 11.9 cm to 30.1 cm. Its current velocity preferences ranged from 9.8 cm s-1 to 29.1 cm s-1. Its substrate preferences ranged from -5.1 𝜱m to -2.5 𝜱m. Median values of central tendency were determined as follows: water depth of 21.4 cm, current velocity of 21.2 cm s-1, and substrate of -4.1 𝜱m. Mean values of central tendency were determined as follows: water depth of 21.8 cm, current velocity of 22.0 cm s-1, and substrate of -4.4 𝜱m. Mode values of central tendency were determined as follows: water depth of 21.7 cm, current velocity of 20.1 cm s-1, and substrate of -3.7 𝜱m. Conclusions: Based on habitat suitability analysis, physical microhabitat characteristics of C. similis within a stream were identified as Run section with coarse particle substrate, low water depth, and slow current velocity. Due to high sensitivity of these habitats to environmental changes, prioritized selection and assessment of threats should be carried out as a primary step.

Development and Validation of a Model Using Radiomics Features from an Apparent Diffusion Coefficient Map to Diagnose Local Tumor Recurrence in Patients Treated for Head and Neck Squamous Cell Carcinoma

  • Minjae Kim;Jeong Hyun Lee;Leehi Joo;Boryeong Jeong;Seonok Kim;Sungwon Ham;Jihye Yun;NamKug Kim;Sae Rom Chung;Young Jun Choi;Jung Hwan Baek;Ji Ye Lee;Ji-hoon Kim
    • Korean Journal of Radiology
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    • v.23 no.11
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    • pp.1078-1088
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    • 2022
  • Objective: To develop and validate a model using radiomics features from apparent diffusion coefficient (ADC) map to diagnose local tumor recurrence in head and neck squamous cell carcinoma (HNSCC). Materials and Methods: This retrospective study included 285 patients (mean age ± standard deviation, 62 ± 12 years; 220 male, 77.2%), including 215 for training (n = 161) and internal validation (n = 54) and 70 others for external validation, with newly developed contrast-enhancing lesions at the primary cancer site on the surveillance MRI following definitive treatment of HNSCC between January 2014 and October 2019. Of the 215 and 70 patients, 127 and 34, respectively, had local tumor recurrence. Radiomics models using radiomics scores were created separately for T2-weighted imaging (T2WI), contrast-enhanced T1-weighted imaging (CE-T1WI), and ADC maps using non-zero coefficients from the least absolute shrinkage and selection operator in the training set. Receiver operating characteristic (ROC) analysis was used to evaluate the diagnostic performance of each radiomics score and known clinical parameter (age, sex, and clinical stage) in the internal and external validation sets. Results: Five radiomics features from T2WI, six from CE-T1WI, and nine from ADC maps were selected and used to develop the respective radiomics models. The area under ROC curve (AUROC) of ADC radiomics score was 0.76 (95% confidence interval [CI], 0.62-0.89) and 0.77 (95% CI, 0.65-0.88) in the internal and external validation sets, respectively. These were significantly higher than the AUROC values of T2WI (0.53 [95% CI, 0.40-0.67], p = 0.006), CE-T1WI (0.53 [95% CI, 0.40-0.67], p = 0.012), and clinical parameters (0.53 [95% CI, 0.39-0.67], p = 0.021) in the external validation set. Conclusion: The radiomics model using ADC maps exhibited higher diagnostic performance than those of the radiomics models using T2WI or CE-T1WI and clinical parameters in the diagnosis of local tumor recurrence in HNSCC following definitive treatment.

Phenotypical Characteristics Investigation and Selection of Superior Individuals from Natural Habitats of Sageretia thea in South Korea (국내 자생 상동나무 표현형 특성조사 및 우량 개체 선발)

  • Dae Hui Jeong;Hae Yun Kwon;Young Ki Kim
    • Korean Journal of Plant Resources
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    • v.37 no.2
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    • pp.214-224
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    • 2024
  • In this study, the growth, physical characteristics of fruit and soil chemistry were investigated by grouping S. thea populations. The soil for each group was sandy loam or sandy loam, and it was analyzed to be slightly acidic to neutral, with a pH ranging from 5.6 to 7.0. Additionally, the leaf size was highest in the G7 group, and overall fruit growth was highest in the G1 group, confirming differences between the groups. The sweetness ranged from 16.8° Brix to 12.3° Brix, indicating a higher sweetness compared to Vaccinium oldhamill, blueberry, and blackberry. The correlation analysis between soil chemistry and S. thea growth characteristics revealed a significant negative correlation between calcium (Ca) and fruit growth characteristics. Furthermore, a significant positive correlation was observed between sodium (Na) and fruit acidity, as well as between cation exchange capacity (CEC) and fruit hardness.

Surgical Outcomes of Cervical Esophageal Cancer: A Single-Center Experience

  • Yoonseo Lee;Jeonghee Yun;Yeong Jeong Jeon;Junghee Lee;Seong Yong Park;Jong Ho Cho;Hong Kwan Kim;Yong Soo Choi;Young Mog Shim
    • Journal of Chest Surgery
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    • v.57 no.1
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    • pp.62-69
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    • 2024
  • Background: Cervical esophageal cancer is a rare malignancy that requires specialized care. While definitive chemoradiation is the standard treatment approach, surgery remains a valuable option for certain patients. This study examined the surgical outcomes of patients with cervical esophageal cancer. Methods: The study involved a retrospective review and analysis of 24 patients with cervical esophageal cancer. These patients underwent surgical resection between September 1994 and December 2018. Results: The mean age of the patients was 61.0±10.2 years, and 22 (91.7%) of them were male. Furthermore, 21 patients (87.5%) had T3 or T4 tumors, and 11 (45.8%) exhibited lymph node metastasis. Gastric pull-up with esophagectomy was performed for 19 patients (79.2%), while 5 (20.8%) underwent free jejunal graft with cervical esophagectomy. The 30-day operative mortality rate was 8.3%. During the follow-up period, complications included leakage at the anastomotic site in 9 cases (37.5%) and graft necrosis of the gastric conduit in 1 case. Progression to oral feeding was achieved in 20 patients (83.3%). Fifteen patients (62.5%) displayed tumor recurrence. The median time from surgery to recurrence was 10.5 months, and the 1-year recurrence rate was 73.3%. The 1-year and 3-year survival rates were 75% and 33.3%, respectively, with a median survival period of 17 months. Conclusion: Patients with cervical esophageal cancer who underwent surgical resection faced unfavorable outcomes and relatively poor survival. The selection of cases and decision to proceed with surgery should be made cautiously, considering the risk of severe complications.

Changes in the Teaching Expertise of Teachers Participating in an In-School Professional Learning Community for Elementary Science Instructional Research (초등과학 수업 연구를 위한 학교 안 전문적 학습공동체 참여 교사들의 수업 전문성 변화 양상)

  • Kim, Eun Seo;Lee, Sun-Kyung
    • Journal of Korean Elementary Science Education
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    • v.43 no.1
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    • pp.185-200
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    • 2024
  • This study explored the changes in the elementary science teaching expertise of teachers who participated in an in-school professional learning community for elementary science instructional research. Six elementary school teachers from grades 4, 5, and 6 at an 18-class S elementary school in a medium-sized city in Chungcheongbuk-do conducted collaborative instructional research on elementary science lessons as part of an in-school professional learning community, which was held 26 times over 7 months in 2020. During the professional learning community, video and audio recordings of the activities, research lessons, course materials, and professional learning community reflection activities were collected for analysis. The collected data were analyzed using qualitative research methods; data processing, reading, note-taking, description, classification, interpretation, reporting, and visualization; and the instructional professionalism elements were extracted based on the instructional professionalism framework. In the early professional learning community activity stages, the participating teachers first discussed their teaching perspectives, their experiences, and their goals for teaching science, which resulted in a selection of research questions. The teachers then collaboratively designed and implemented research lessons for each grade level, after which lesson reflections were conducted. The teachers' abilities to engage in qualitative reflection on the research questions improved after each reflection iteration. It was found that this professional learning community collaborative lesson study experience positively contributed to teaching expertise development. Based on the study findings, the implications for using professional learning communities to improve elementary teachers' science teaching expertise are given.