• Animal Bioscience
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• 제35권6호
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• pp.869-883
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• 2022

Objective: The aim of study was to investigate the effects of in-feed supplementation of Bacillus amyloliquefaciens (BA) and Saccharomyces cerevisiae (SC) on growth performance, gut integrity, and microbiota modulations in red-feathered native chickens (RFCs). Methods: A total of 18,000 RFCs in a commercial farm were evenly assigned into two dietary treatments (control diet; 0.05% BA and 0.05% SC) by randomization and raised for 11 weeks in two separate houses. Fifty RFCs in each group were randomly selected and raised in the original house with the partition for performance evaluations at the age of 9 and 11 weeks. Six non-partitioned RFCs per group were randomly selected for analyses of intestinal architecture and 16S rRNA metagenomics. Results: Feeding BA and SC increased the body weight and body weight gain, significantly at the age of 11 weeks (p<0.05). The villus height/crypt ratio in the small intestines and Firmicutes to Bacteroidetes ratio were also notably increased (p<0.05). The supplementation did not disturb the microbial community structure but promote the featured microbial shifts characterized by the significant increments of Bernesiella, Prevotellaceae_NK3B31_group, and Butyrucimonas, following remarkable decrements of Bacteroides, Rikenellaceae_RC9_gut_group, and Succinatimonas in RFCs with growth benefits. Besides, functional pathways of peptidoglycan biosynthesis, nucleotide excision repair, glycolysis/gluconeogenesis, and aminoacyl transfer ribonucleic acid (tRNA) biosynthesis were significantly promoted (p<0.05). Conclusion: In-feed supplementation of BA and SC enhanced the growth performance, improved mucosal architectures in small intestines, and modulated the cecal microbiota and metabolic pathways in RFCs.

• 한국동물위생학회지
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• 제31권4호
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• pp.497-504
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• 2008

A cross-sectional study was undertaken to report prevalence and to identify risk factors of subclinical mastitis of dairy cattle in Sylhet district of Bangladesh. Among 325 dairy farms of the district 12 farms(3.7%) were selected conveniently for this study. All the dairy cows of the 12 farms were selected for sample collection. Fresh milk samples from each of the selected dairy cows were collected aseptically in separate sterilized test tube as RF, RH, LF and LH quarter of the udder. Rapid modified White Side Test(WST) was used to detect subclinical mastitis(SCM). Results of WST and data derived from filled in questionnaire were entered in Microsoft Excel 2003 and transferred to $STATA^{(R)}$, version 8.0/Intercooled(Stata Corporation, Texas, USA, 2003). The overall prevalence of SCM and its distribution in different categories of variables in cow and their exact binomial 95% confidence intervals were calculated in $STATA^{(R)}$. Simple bivariable associations among independent variables were investigated by $x^2$ test in $STATA^{(R)}$. Multiple logistic regression analysis with backward elimination method was used to identify risk factors of SCM. To identify significant variation in quarter SCM, linear regression analysis was performed after arcsine transformation of the data. The overall prevalence of SCM found in this study is 54%. Dairy cows with teat lesions had significantly increased SCM(OR=12342, P value=0.000, 95% CI=762, 199798) than others without teat lesions. The Holstein Friesian X Jersey X Sahiwal breed has significantly decreased(OR=0.18, p=0.03, 95% CI 0.04, 0.85) SCM than other breeds. The prevalence of SCM found in this study is in agreement with others. The injury in the teat increases the probability of getting infected with microbes and thereby mastitis. If the prevalence of teat lesion can be decreased the probability of subclinical mastitis will also be decreased. The negatively associated Holstein Friesian X Jersey X Sahiwall breed may help in planning mastitis control program if this finding can be validated by a more powerful case-control or cohort study design.

• Clinical and Experimental Reproductive Medicine
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• 제41권4호
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• pp.165-167
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• 2014

Objective: The objective of this study is to estimate the effects of Inclear, a feminine cleanser, on sperm motility. Methods: Semen samples were obtained from infertile male patients. Following liquefaction, the raw semen samples were diluted with Ham's F-10 nutrient mixture medium containing 0.4% human serum albumin solution at a ratio of 1:3. The semen samples were subsequently centrifuged to separate the seminal plasma from the serum. The supernatant was discarded, and the pellet was resuspended. The sample was again centrifuged to remove cell debris, and the supernatant was removed. The final pellet was gently loosened by resuspension and incubated in medium alone as a control, and in a 10% solution of the medium plus Inclear. A sampling time of 30 minutes was selected on the basis of sperm transport studies. Sperm motility was evaluated with computer-assisted sperm analysis. Results: A total of 20 samples were analyzed. The mean age of patients was $34.40{\pm}2.96years$. There was no difference in sperm concentration and motility in the two samples at 0 minute and 30 minutes of incubation. In both semen samples, the sperm concentration and motility decreased after an incubation period of 30 minutes. However, there was no statistical difference between the samples. Sperm concentration and motility were not significantly different between the control and Inclear samples after 0 minute and 30 minutes of incubation. Conclusion: Inclear has no negative effects on sperm motility. This product can be recommended to pregnancy planners for vaginal hygiene and as a vaginal lubricant.

• 한국유기농업학회지
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• 제21권4호
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• pp.725-736
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• 2013

본 실험에 사용한 C. gloeosporioides는 밤나무 재배단지에서 탄저병 증상을 보이는 밤나무 종실로부터 분리하였고, ITS 영역 유전자의 염기서열 및 형태하적 분석을 통하여, C. gloeosporioides로 동정하였다. 병원성 검정실험에서, 80% Eucalyptus oil 및 B. subtilis GG95 10배액 처리구가 C. gloeosporioides에 대한 항균효과가 우수하였고, 최종배합은 Eucalyptus oil 40%, B. subtilis GG95 40% 외 제제화용 첨가제 20%로 하였다. 2012년도 포장실험에서, 자봉 품종 시제품 500배 4회 처리구의 방제가는 무처리구 대비 60.9%로 조사되었고, 2013년도 포장실험에서, 자봉 품종의 방제가는 250배 처리구가 71.2%로 가장 높았다. 조생종 및 중생종보다 만생종에서의 방제가 69.8%로 가장 높은 것으로 보아, 착과이후 종실이 작을 때부터 약제 처리를 하면, 방제가를 높일 수 있을 것으로 기대된다. 포장 500배 4회 처리후 수확물인 조생종 단택에서는 $4^{\circ}C$ 저장에서의 방제효과는 약 61.9%로 조사되었고, $25^{\circ}C$ 저장에서의 방제효과는 63.2%의 방제가로 조사되었고, 실내 단태 품종에 대한 방제가는, 250배에서 81.16%, 500배에서 75.36%로 측정되었으나, 1000배의 효과는 낮은 편이므로, 최소한 500배 이상의 처리가 필요시 된다. 상기의 결과를 바탕으로 밤나무 종실에 발생하는 탄저병을 효율적으로 방제하기 위해서는 착과 이후 개발제품을 500배 희석농도로 4회 경엽처리하고 수확후 250배로 침지 처리한 후 저장하는 것이 가장 효율적인 방법으로 판단된다.

• 융합신호처리학회논문지
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• 제22권3호
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• pp.91-98
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• 2021

기초과학연구원 중이온가속기구축사업단에서는 희귀동위원소(Rare Isotopes, RI) 빔의 생성 및 분리를 위한 ISOL(Isotope Separation On Line) 시스템의 설치 및 성능시험이 진행 중에 있다. ISOL 표적/이온원에서 생성된 다양한 RI 빔은 빔 전송장치 및 분리 장치를 거쳐 사용자가 원하는 RI 빔만 선별되어 ISOL 후단의 초전도 선형가속기로 전송된다. ISOL 시스템에서는 특정 RI 빔을 분리하기 위해 두 개의 분리 장치가 설치되어 있으며 EPICS(Experimental Physics and Industrial Control System)에 의해 제어가 이루어진다. 본 연구에서는 ISOL RI 빔 분리 장치 중 하나인 A/Q 분리기에서 다가(n+) RI 빔의 질량선별을 위해 이극자석의 자기장을 측정하기 위한 EPICS IOC(Input-Output Control)를 개발하였으며, 홀 프로브(Hall Probe)를 이용한 자기장 측정 실험을 통해 A/Q 분리기의 운전 안정성에 대해 평가하였다.

• 대한후두음성언어의학회지
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• 제15권2호
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• pp.81-86
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• 2004

Background and Objectives : In general, ambulatory 24-hour pH monitoring is considered the current gold standard for larynogopharyngeal reflux(LPR). There is no validated instrument whose purpose is to document the physical finding and severity of laryngopharyngeal reflux. The purposes of this study are to revaluate the validity and reliability of the reflux finding score(RFS) and to quantify laryngoscopic findings using reflux finding score. Material and Methods : Thirty-three LPR patients confirmed by dual-probe pH monitoring and thirty patients of control were selected. The RFS was documented for each patient with telescopic laryngoscopy before treatment. For test-retest intraobserver reliability assessment, a blinded laryngologists determined the RFS on two separate occasions. To evaluate interobserver reliability assessment, the RFS was determined by t재 different blinded laryngologists. Results : The mean age of the cohort with pH-documented LPR was 45.8 years and the mean RFS was 11.4. The mean age of cotrol subjects was 52 years and the mean RFS was 5.4. The mean RFS for laryngologist no. 1 was 10.8 at the initial screening and 10.9 at the repeat evaluation. The mean FRS for laryngologist no.2 was 11.1 at the intial test and 10.9 at the repeat evaluation. The correlation coefficient for interobserver variability was 0.93 and intraobserver variability was 0.94. Conclusion : The RFS demonstrates excellent inter-and introaobserver reproducibility and is helpful for quantifying laryngeal finding in LPR. We can be 95% certain that an individual with a RFS greater than 7 has LPR.

• 한국지능시스템학회논문지
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• 제25권6호
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• pp.621-628
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• 2015

본 논문에서는 컴퓨터 비전 및 영상처리 기술을 접목하여 지능형 차량에 적용할 수 있는 실시간 차량 검출 알고리즘을 제안한다. 도로 환경의 빠르게 변화하는 배경과 차량의 다양성 때문에 차량의 실시간 검출은 부정확성 및 계산량 증가의 어려움을 가지고 있다. 본 논문은 기존 방법들의 이러한 문제점들을 해결하기 위하여 먼저, 복잡한 배경이 포함되어 있는 실시간 입력 영상으로부터 수직 에지 정보와 차량의 그림자 색정보를 사용하여 후보군을 검출한다. 다음으로, 검출된 후보군 영역들로부터 HOG 특징점을 추출한 후, 마지막으로 추출된 특징점들을 단일층 전방향 신경망 구조를 기반으로 하는 OS 퍼지-ELM을 사용하여 분류한다. 본 논문에서 제안된 방법을 사용하여 실험을 수행한 결과로써 기존의 ELM 및 OS-ELM 방법보다 계산량 및 정확성면에서 향상되었음을 보인다.

• 물과 미래
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• 제8권1호
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• pp.61-79
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• 1975

본연구는 1974년 건설부 수자원국의 연구사업중의 하나인 $\ulcorner$홍수량 추정을 위한 합성단위유량도유도의 연구$\Ircorner$의 일부로서 건설부에서 제공한 재정적 후원에 감사를 드린다.

• 인터넷정보학회논문지
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• 제10권6호
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• pp.159-171
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• 2009

본 연구의 목적은 초등학교 교육과정에서 활용이 가능한 학습도구로서 프로그래밍이 가능한 로봇의 활용 방안을 알아보기 위함이다. 로봇은 다른 디지털 교구와는 달리 프로그래밍과 이를 통한 조작이 쉽다는 점에서 교수-학습목적의 차별화된 매체적인 특성을 갖는다. 이 연구에서는 초등학교 학생들에게 컴퓨터 프로그램의 핵심인 알고리즘을 가르치기 위한 도구로서 로봇게임프로젝트 개발을 통해 연구목적을 달성하고자 하였다. 본 연구에서 교구로 사용한 레고는 프로그래밍을 할 수 있는 환경을 갖추고 있으며, 중앙제어장치, 센서 및 모터세트를 기계적으로 조합하여 로봇을 만들 수 있다. 개발한 프로젝트는 학생들이 6차시 수업을 통해 3개의 과제를 해결하도록 하는 과정에서 이루어졌다. 이들 각각은 알고리즘의 기본적 원리들을 바탕으로 구성되었으며, 이 프로그램을 교실수업에 적용해 본 결과, 학생들이 상대적으로 학습의 즐거움을 경험하고 몰입의 효과가 나타났음을 알 수 있었다. 이러한 결과는 일반적인 초등학교 교실에서 직접 로봇을 조작하는 활동이 학교교육활동에 새로운 가능성을 가져올 수 있음을 보여준 것이라고 하겠다.

• 의료ㆍ복지 건축 : 한국의료복지건축학회 논문집
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• 제28권2호
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• pp.31-38
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• 2022

Purpose: This study presents the results of the analysis on space utilization of kidney dialysis units in regional public hospitals, which plays a key role in local public medical services. The result aims to achieve safety from infection, allow comfort for the dialysis environment, and stability for medical support. The purpose of this study is to present fundamental data for architectural plans for the kidney dialysis unit, as well as to alleviate potential infectious diseases such as COVID-19. Methods: For research purposes, the investigation and analysis of space utilization were based on architectural floor plans, research papers and literature, related legal systems, and public statistics. Of the main 35 regional public hospitals, in regards to data accessibility, 15 facilities were selected to conduct the survey and analysis for the objective. Results: The space composition by area research results of kidney dialysis units in public hospitals are as follows: Firstly, most targets do not have required rooms in the access and support area, except for the hemodialysis beds in the treatment section. Secondly, the access area requires necessary room and space design that took into consideration of convenience and accessibility for patients. Thirdly, in regards to infection prevention and control, proper circulation and room plan is essential for storage and disposal of contaminated products and linen after use. For the treatment area, the arrangement plan needs to establish a visual connection between the isolation room, the nursing station, and the bed area. Additionally, consideration of circulation in the preparation, treatment, observation, examination, and all other rooms in the facility is required. Lastly, for the support area, the room is designed to consider adequate working and meeting spaces for the medical staff, consultation space for patients or guardians, separate storage and disposal of clean and contaminated items, and the storage of various equipment for dialysis. Implications: In preparation for the increase in chronic kidney failure patients and the spread of infectious diseases, such as COVID-19, the researched data demonstrates the basic guidelines for space composition of kidney dialysis units and the significant role of regional public hospitals.