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Bacillus amyloliquefaciens and Saccharomyces cerevisiae feed supplements improve growth performance and gut mucosal architecture with modulations on cecal microbiota in red-feathered native chickens

  • Lee, Tzu-Tai;Chou, Chung-Hsi;Wang, Chinling;Lu, Hsuan-Ying;Yang, Wen-Yuan
    • Animal Bioscience
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    • v.35 no.6
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    • pp.869-883
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    • 2022
  • Objective: The aim of study was to investigate the effects of in-feed supplementation of Bacillus amyloliquefaciens (BA) and Saccharomyces cerevisiae (SC) on growth performance, gut integrity, and microbiota modulations in red-feathered native chickens (RFCs). Methods: A total of 18,000 RFCs in a commercial farm were evenly assigned into two dietary treatments (control diet; 0.05% BA and 0.05% SC) by randomization and raised for 11 weeks in two separate houses. Fifty RFCs in each group were randomly selected and raised in the original house with the partition for performance evaluations at the age of 9 and 11 weeks. Six non-partitioned RFCs per group were randomly selected for analyses of intestinal architecture and 16S rRNA metagenomics. Results: Feeding BA and SC increased the body weight and body weight gain, significantly at the age of 11 weeks (p<0.05). The villus height/crypt ratio in the small intestines and Firmicutes to Bacteroidetes ratio were also notably increased (p<0.05). The supplementation did not disturb the microbial community structure but promote the featured microbial shifts characterized by the significant increments of Bernesiella, Prevotellaceae_NK3B31_group, and Butyrucimonas, following remarkable decrements of Bacteroides, Rikenellaceae_RC9_gut_group, and Succinatimonas in RFCs with growth benefits. Besides, functional pathways of peptidoglycan biosynthesis, nucleotide excision repair, glycolysis/gluconeogenesis, and aminoacyl transfer ribonucleic acid (tRNA) biosynthesis were significantly promoted (p<0.05). Conclusion: In-feed supplementation of BA and SC enhanced the growth performance, improved mucosal architectures in small intestines, and modulated the cecal microbiota and metabolic pathways in RFCs.

Prevalence and risk factors of subclinical bovine mastitis in some dairy farms of Sylhet district of Bangladesh

  • Kahir, Md. Abdul;Islam, Md. Mazharul;Rahman, A.K.M. Anisur;Nahar, A.;Rahman, Md. Siddiqur;Son, Hee-Jong
    • Korean Journal of Veterinary Service
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    • v.31 no.4
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    • pp.497-504
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    • 2008
  • A cross-sectional study was undertaken to report prevalence and to identify risk factors of subclinical mastitis of dairy cattle in Sylhet district of Bangladesh. Among 325 dairy farms of the district 12 farms(3.7%) were selected conveniently for this study. All the dairy cows of the 12 farms were selected for sample collection. Fresh milk samples from each of the selected dairy cows were collected aseptically in separate sterilized test tube as RF, RH, LF and LH quarter of the udder. Rapid modified White Side Test(WST) was used to detect subclinical mastitis(SCM). Results of WST and data derived from filled in questionnaire were entered in Microsoft Excel 2003 and transferred to $STATA^{(R)}$, version 8.0/Intercooled(Stata Corporation, Texas, USA, 2003). The overall prevalence of SCM and its distribution in different categories of variables in cow and their exact binomial 95% confidence intervals were calculated in $STATA^{(R)}$. Simple bivariable associations among independent variables were investigated by $x^2$ test in $STATA^{(R)}$. Multiple logistic regression analysis with backward elimination method was used to identify risk factors of SCM. To identify significant variation in quarter SCM, linear regression analysis was performed after arcsine transformation of the data. The overall prevalence of SCM found in this study is 54%. Dairy cows with teat lesions had significantly increased SCM(OR=12342, P value=0.000, 95% CI=762, 199798) than others without teat lesions. The Holstein Friesian X Jersey X Sahiwal breed has significantly decreased(OR=0.18, p=0.03, 95% CI 0.04, 0.85) SCM than other breeds. The prevalence of SCM found in this study is in agreement with others. The injury in the teat increases the probability of getting infected with microbes and thereby mastitis. If the prevalence of teat lesion can be decreased the probability of subclinical mastitis will also be decreased. The negatively associated Holstein Friesian X Jersey X Sahiwall breed may help in planning mastitis control program if this finding can be validated by a more powerful case-control or cohort study design.

Effects of feminine cleanser Inclear on sperm motility: A prospective study

  • Park, Dong-Wook;An, Jin Hee;Han, Sang Chul;Lee, Jongwon;Lee, Hyo Serk;Seo, Ju Tae
    • Clinical and Experimental Reproductive Medicine
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    • v.41 no.4
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    • pp.165-167
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    • 2014
  • Objective: The objective of this study is to estimate the effects of Inclear, a feminine cleanser, on sperm motility. Methods: Semen samples were obtained from infertile male patients. Following liquefaction, the raw semen samples were diluted with Ham's F-10 nutrient mixture medium containing 0.4% human serum albumin solution at a ratio of 1:3. The semen samples were subsequently centrifuged to separate the seminal plasma from the serum. The supernatant was discarded, and the pellet was resuspended. The sample was again centrifuged to remove cell debris, and the supernatant was removed. The final pellet was gently loosened by resuspension and incubated in medium alone as a control, and in a 10% solution of the medium plus Inclear. A sampling time of 30 minutes was selected on the basis of sperm transport studies. Sperm motility was evaluated with computer-assisted sperm analysis. Results: A total of 20 samples were analyzed. The mean age of patients was $34.40{\pm}2.96years$. There was no difference in sperm concentration and motility in the two samples at 0 minute and 30 minutes of incubation. In both semen samples, the sperm concentration and motility decreased after an incubation period of 30 minutes. However, there was no statistical difference between the samples. Sperm concentration and motility were not significantly different between the control and Inclear samples after 0 minute and 30 minutes of incubation. Conclusion: Inclear has no negative effects on sperm motility. This product can be recommended to pregnancy planners for vaginal hygiene and as a vaginal lubricant.

Biological Control & Isolation of Chestnut Diseases by Colletotrichum Goeosporioides (밤나무 종실에 발생하는 탄저병균의 분리 및 친환경적 방제)

  • Kim, Young-Jae;Kang, Kil-Nam;Kim, Jong-Woo;Kim, Young-Myung;Lee, Sang-Hyeon;Hong, Suk-Il;Lee, Jae-Jun;Lee, Hyun-Su;Kim, Young-Kwon;Park, In-Seo;Cho, Yong-Gu
    • Korean Journal of Organic Agriculture
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    • v.21 no.4
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    • pp.725-736
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    • 2013
  • In order to acquire morphological characteristics and genetic characteristics of pathogen that causes anthrax to chestnut, anthrax was separate and identified in Gongju, chungnam chestnut plantation. Antagonistic microorganisms and plant extracts were selected for control of anthrax. Medium maturing variety treatment of 250 dilution fold in field was control at 71.2% and treatment of 500 dilution fold was control at 64.4% and treatment of 1000 dilution fold was control at 40.7%. Storage control value of Jabong in $25^{\circ}C$ after treatment in field is 61.7% at 250 dilution fold, 62.8% at 500 dilution fold, 40.9% at 1000 dilution fold treatment.

Development of EPICS-IOC Measuring Magnetic Field at A/Q separator for Separating Specific Ions (가속이온 분리를 위한 A/Q Separator에서 자장측정용 EPICS-IOC 개발)

  • Lee, Su-Yeong;Yim, Hee-Joong;Kim, Jae-Hong;Mun, Jun-Yeong;Park, Mi-Jeong;Lee, Sang-Il;Lee, Dong-Hoon
    • Journal of the Institute of Convergence Signal Processing
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    • v.22 no.3
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    • pp.91-98
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    • 2021
  • The installation and performance test of the ISOL (Isotope Separation On Line) system for the generation and separation of Rare Isotopes (RI) beams is in progress at the Rare Isotope Science Project (RISP), Institute for Basic Science (IBS). The various RI beams generated by the ISOL target/ion source go through the beam lines and separators, and only the RI beam desired by the user is selected and transmitted to the superconducting linear accelerator at the downstream of the ISOL. In the ISOL system, two separators are installed to separate a specific RI beam, and control is performed by the Experimental Physics and Industrial Control System (EPICS). In this study, an EPICS IOC (Input-Output Control) was developed to measure the magnetic field of a dipole magnet for mass separation of a multivalent (n+) RI beam in the A/Q separator, which is one of the ISOL RI beam separators. The operational stability of the A/Q separator was tested through a magnetic field measurement using a Hall probe.

Revaluation of Reflux Finding Score(RFS) in Laryngopharyngeal Reflux(LPR) (인후두역류증의 진단에 있어서 후두내시경검사 소견 점수화의 유용성에 대한 재검증)

  • Kwon, Kee-Hwan;Ban, Jae-Ho;Lee, Kyung-Chul
    • Journal of the Korean Society of Laryngology, Phoniatrics and Logopedics
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    • v.15 no.2
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    • pp.81-86
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    • 2004
  • Background and Objectives : In general, ambulatory 24-hour pH monitoring is considered the current gold standard for larynogopharyngeal reflux(LPR). There is no validated instrument whose purpose is to document the physical finding and severity of laryngopharyngeal reflux. The purposes of this study are to revaluate the validity and reliability of the reflux finding score(RFS) and to quantify laryngoscopic findings using reflux finding score. Material and Methods : Thirty-three LPR patients confirmed by dual-probe pH monitoring and thirty patients of control were selected. The RFS was documented for each patient with telescopic laryngoscopy before treatment. For test-retest intraobserver reliability assessment, a blinded laryngologists determined the RFS on two separate occasions. To evaluate interobserver reliability assessment, the RFS was determined by t재 different blinded laryngologists. Results : The mean age of the cohort with pH-documented LPR was 45.8 years and the mean RFS was 11.4. The mean age of cotrol subjects was 52 years and the mean RFS was 5.4. The mean RFS for laryngologist no. 1 was 10.8 at the initial screening and 10.9 at the repeat evaluation. The mean FRS for laryngologist no.2 was 11.1 at the intial test and 10.9 at the repeat evaluation. The correlation coefficient for interobserver variability was 0.93 and intraobserver variability was 0.94. Conclusion : The RFS demonstrates excellent inter-and introaobserver reproducibility and is helpful for quantifying laryngeal finding in LPR. We can be 95% certain that an individual with a RFS greater than 7 has LPR.

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Vision-based Vehicle Detection Using HOG and OS Fuzzy-ELM (HOG와 OS 퍼지-ELM를 이용한 비전 기반 차량 검출 시스템)

  • Yoon, Changyong;Lee, Heejin
    • Journal of the Korean Institute of Intelligent Systems
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    • v.25 no.6
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    • pp.621-628
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    • 2015
  • This paper describes an algorithm for detecting vehicles detection in real time. The proposed algorithm has the technique based on computer vision and image processing. In real, complex environment such as one with road traffic, many algorithms have great difficulty such as low detection rate and increasing computational time due to complex backgrounds and rapid changes. To overcome this problem in this paper, the proposed algorithm consists of the following methods. First, to effectively separate the candidate regions, we use vertical and horizontal edge information, and shadow values from input image sequences. Second, we extracts features by using HOG from the selected candidate regions. Finally, this paper uses the OS fuzzy-ELM based on SLFN to classify the extracted features. The experimental results show that the proposed method perform well for detecting vehicles and improves the accuracy and the computational time of detecting.

A Synthesis of Unit Hydrograph by a Correlation Analysis between the Basin Characteristics and the Runoff-Characteristics - Han and Geum River Basin - (유역특성과 유출특성간의 상관관계 해석에 의한 단위유량도의 합성 - 한강 및 금강유역 -)

  • 윤용남;선우중호
    • Water for future
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    • v.8 no.1
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    • pp.61-79
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    • 1975
  • An attempt is made to develope a scheme for synthesizing unit hydrograph for any arbitrary small watershed in the Han or Geum River basin, which can be applied in determining various sizes of design flood for flood control prijects. Stage gauging stations, seven in the Han and five in the Geun River basin with rating curves, were selected as subbasins for the analysis. Unit hydrographs of 2-hour duration were derived for several heavy storm events using the storm and the corresponding flood runoff data for each subbasin. The Clark method programmed by the Hydrologic Engineering Center, U.S. Corps of Engineers, was utilized for derivation of instantaneous unit hydrographs which were, in turn, converted into 2-hour unit hydrograph. By averaging the 2-hour unit hydrographs from several storm events a representative 2-hour unit hydrograph was determined for each subbasin and hence a separate derivation of dimensionless unit hedrograph was also possible for the Han and Geum River basins. The physiographic characteristics such as stream length, distance to the centroid of each watershed were correlated with the characteristic parameters of the derived unit hydrograph for the subbasins within two large basins. correlation analyses between the characteristic parameters were also made. These correlation analyses resulted a series of four equations and a dimensionless unit hydrograph for the two large basins, which made it possible to draw a synthetic 2-hour unitgraph for any small watershed within the Han or Geum River basin. A detailed procedure for aplying the derived method for an arbitrary basin is summarized with one sample computation for each of the two basins. A comparison of the actually derived 2-hour unit hydrogrograph and the synthesized one showed a fair agreement. A recommendation is made for the further study.

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A Study on the Design and Development of Robot Game-based Project for Teaching Children to Program Computers (프로그램교육 목적의 로봇게임 프로젝트 학습 구안에 관한 연구)

  • Shin, Seung-Young;You, Sang-Mi;Kim, Mi-Ryang
    • Journal of Internet Computing and Services
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    • v.10 no.6
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    • pp.159-171
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    • 2009
  • The objective of this research is to explore a method to utilize a programmable robot, as a potential learning tool in the elementary school's curricula. Due to their programmability and operational ease of use, programmable robots are among digital toys that today offer specially instructive features. In this research, we developed the robot game-based project contents as a tool for teaching the elementary school children to learn the algorithm, the essential part of computer programming. The LEGO material, selected as the construction kit for robot, consists of a mechanical assembly system, a set of sensors and actuators, a central control unit, a programming environment. The project requires the children to complete 3 separate tasks, each of which is developed based on the principles of algorithm. The classroom feedback supports that the robotic experiences provided the children with fun and absorption. It is likely that implementing learning with robot in regular classroom in elementary school can bring new possibilities to the educational system, provided that a thorough preparation backs up the plan.

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A Study on the Space Composition for Department of Kidney Dialysis in Regional Public Hospital(1) (지역거점 공공병원의 인공신장부 공간구성에 관한 연구(1))

  • Chai, Choul Gyun;Park, Kyeong Hyeon
    • Journal of The Korea Institute of Healthcare Architecture
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    • v.28 no.2
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    • pp.31-38
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    • 2022
  • Purpose: This study presents the results of the analysis on space utilization of kidney dialysis units in regional public hospitals, which plays a key role in local public medical services. The result aims to achieve safety from infection, allow comfort for the dialysis environment, and stability for medical support. The purpose of this study is to present fundamental data for architectural plans for the kidney dialysis unit, as well as to alleviate potential infectious diseases such as COVID-19. Methods: For research purposes, the investigation and analysis of space utilization were based on architectural floor plans, research papers and literature, related legal systems, and public statistics. Of the main 35 regional public hospitals, in regards to data accessibility, 15 facilities were selected to conduct the survey and analysis for the objective. Results: The space composition by area research results of kidney dialysis units in public hospitals are as follows: Firstly, most targets do not have required rooms in the access and support area, except for the hemodialysis beds in the treatment section. Secondly, the access area requires necessary room and space design that took into consideration of convenience and accessibility for patients. Thirdly, in regards to infection prevention and control, proper circulation and room plan is essential for storage and disposal of contaminated products and linen after use. For the treatment area, the arrangement plan needs to establish a visual connection between the isolation room, the nursing station, and the bed area. Additionally, consideration of circulation in the preparation, treatment, observation, examination, and all other rooms in the facility is required. Lastly, for the support area, the room is designed to consider adequate working and meeting spaces for the medical staff, consultation space for patients or guardians, separate storage and disposal of clean and contaminated items, and the storage of various equipment for dialysis. Implications: In preparation for the increase in chronic kidney failure patients and the spread of infectious diseases, such as COVID-19, the researched data demonstrates the basic guidelines for space composition of kidney dialysis units and the significant role of regional public hospitals.