• Title/Summary/Keyword: Segregation effect

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Curing and segregation of pSL100 and recombination of its segregants (Plasmid pSL100의 curing, segregation 및 segregants 들의 재조합에 관한 연구)

  • 백형석;김국찬;이세영
    • Korean Journal of Microbiology
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    • v.20 no.1
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    • pp.11-20
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    • 1982
  • A study was undertaken to examine the effect of curing agents on the stability, curing and segregation of R plasmid pSL100. And also the stability, transfer frequency, and recombination of its segregants obtained from curing agent treatment were studied. Ethidium bromide, acridine orange, and mitomycin-C were used as curing agent. The results obtained were as follows ; 1. The curing agent ethidium bromide, acridine orange, and mitomycin-C were not effective for curing the multiple antibiotic resistant determinant of pSL100 in Salmonella typhimurium and Escherichia coli. However, they induced plasmid segregation with high frequency in S.typhimuruim LT-2strains. TcApSmCm, TcSmCmKm, TcApCm, TcAp, TcKm, Tc segregants were obtained. 2. The resistant markers of the segregents were transferred to S.typhimurium LT-2 strains with high frequencies whereas they were transferred to E.coli K-12 only with low frequencies. 3. The transconjugants obtained from conjugation between two different S.typhimurium segregants were similar to the phenotype of the original R factor pSL100 and the resistant markers were transferred to the S.typhimurium LT-2 or E.coli strain with equal frequencies, indicating that they are recombinants. 4. The transconjugants obtained from conjugation between pSL100 segrgants and pKM101, or pBR322 possessed the resistant markers of the two parental plasmids and they were transferred to both S.typhimurium and E.coli K-12 strains with the same frequencies and maintained stably, suggesting that they are also recombinants. 5. The recombinant pSL100 could be also obtained in rec A-strains of E.coli, suggesting that the gene function of rec A is required for the recombination of pSL100 segregants in E.coli.

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Effect of Heat Treatment on the Corrosion Properties of Seamless 304L Stainless Steel Pipe (이음매 없는 304L 스테인리스강관의 부식특성에 미치는 열처리의 영향)

  • Kim, K.T.;Um, S.B.;Kim, Y.S.
    • Corrosion Science and Technology
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    • v.16 no.6
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    • pp.305-316
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    • 2017
  • Austenitic stainless steels have been widely used for various systems of nuclear power plants. Among these stainless steels, small pipes with diameter less than 14 inch have been produced in the form of seamless pipe. Annealing and cooling process during the manufacturing process can affect corrosion properties of seamless stainless steels. Therefore, 12 inch-diameter of as-received 304L stainless steel pipe was annealed and aged in this study. Intergranular corrosion resistance was evaluated by ASTM A262 Practice A, C, and E methods. The degree of sensitization was determined using a DL-EPR test. U-bend method in an autoclave was used to evaluate the SCC resistance in 0.01 M $Na_2S_4O_6$ or 40% NaOH solution at $340^{\circ}C$. As-received specimen showed relatively high degree of sensitization and intergranular corrosion rate. Carbon segregation was also observed near grain boundaries. Annealing treatment could give the dissolution of segregated carbon into the matrix. Aging treatment could induce segregation of carbon and finally form carbides. Microstructural analysis confirmed that high intergranular corrosion rate of the as-received seamless pipe was due to micro-galvanic corrosion between carbon segregation and grains.

Effect of Carbon on the Coefficient of Thermal Expansion of As-Cast Fe-3 0 wt.%Ni-12.5wt.% Co-xC Invar Alloys

  • 김봉서;유경재;김병걸;이희웅
    • Transactions of Materials Processing
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    • v.8 no.3
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    • pp.247-247
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    • 1999
  • The segregation (distribution) of nickel and the composition of its constituents influence the low thermal expansion characteristics (Invar effect) in Fe-30 wt.% Ni-12.5 wt.% Co-xC Invar alloy. The change of coefficient of the thermal expansion and magnetic properties were studied as an aspect of carbon addition causing the segregation of Ni in primary austenite of as-cast Fe-30 wt.% Ni-12.5 wt.% Co Invar alloy. The coefficient of thermal expansion of Fe-30 wt.% Ni-12.5 wt.% Co-xC Invar alloy showed its lowest value at 0.08 wt.% carbon, increased with increasing carbon content in the range of 0.08-1.0 wt.%C, kept constant at 1.0-2.0 wt.%C and decreased at carbon higher than 2.0 wt.%. The effective distribution of the coefficient of nickel in as-cast Fe-30 wt.% Ni-12.5 wt.% Co-xC Invar alloy increased with increasing carbon content. The volume fraction of they phase of Fe-30 wt.% Ni-12.5 wt.% Co-xC alloy increased with increasing carbon content. The microstructure of Fe-30 wt.% Ni-12.5 wt.% Co-xC alloy changed with the carbon content was independent of the coefficient of thermal expansion. The Curie temperature changed linearly with the carbon content and was similar to the change of the coefficient of thermal expansion. Moreover, the coefficient of thermal expansion decreased when the ratio of saturation magnetization to Curie temperature ($\sigma_s/T_c$) increased, decreasing the Curie temperature and showed a specific relationship with the magnetic properties of the Fe-30 wt.% Ni-12.5 wt.% Co-xCInvar alloy.

Numerical analysis of the continuous casting process in the presence of thermo-solutal convection (열농도대류를 고려한 연속주조공정의 수치해석)

  • Jeong, Jae-Dong;Yu, Ho-Seon;Lee, Jun-Sik
    • Transactions of the Korean Society of Mechanical Engineers B
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    • v.21 no.3
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    • pp.445-456
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    • 1997
  • Continuous casting process is numerically analyzed using the continuum model in a non-orthogonal coordinate system. Flow damping in the mush is modeled by combining the viscosity dependence on liquid fraction in dilute mush and the permeability dependence on liquid fraction in concentrated mush. The effect of turbulence is indirectly considered by effective diffusivity determined elsewhere by experiment. The main objective is to investigate the effects of casting parameters such as casting speed and tundish superheat on the distribution of surface temperature, shell thickness, metallurgical length and centerline segregation. Some of the computed results are compared with available experiments, and reasonable agreements are obtained.

The effect of the injection condition on liquid segregation in semi-solid die casting process (반응고 다이캐스팅공정에서 사출조건이 액상편석에 미치는 영향)

  • 신현기;서판기;강충길
    • Proceedings of the Korean Society for Technology of Plasticity Conference
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    • 2003.05a
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    • pp.70-73
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    • 2003
  • Semi-solid processing is now becoming of great interest for the production of various parts by pressure die casting. Many advantages are associated with this forming process at the condition that the forming operation is performed under appropriate conditions in terms of alloy microstructure, injection velocity and pressure. In this study, to look into the relation along them, the experiment of semi-solid die casting has been peformed with various condition of injection, and investigated their microstrucure and solid fraction

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Temperature-dependent Morphology of Self-assembled InAs Quantum Dots Grown on Si Substrates (Si 기판 위에 형성된 InAs 양자점의 열처리에 의한 표면 상태의 변화)

  • Yoo, Choong-Hyun
    • Journal of the Korean Institute of Electrical and Electronic Material Engineers
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    • v.20 no.10
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    • pp.864-868
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    • 2007
  • Effect of high-temperature annealing on morphology of fully coherent self-assembled InAs quantum dots' grown on Si (100) substrates at $450^{\circ}C$ by atmospheric pressure metalorganic chemical vapor deposition(APMOCVD) was investigated by atomic force microscopy(AFM). When the dots were annealed at 500 - 600$^{\circ}C$ for 15 sec - 60 min, there was no appreciable change in the dot density but the heights of the dots increased along with the reduction in the diameters. In segregation from the InAs quantum dots and/or from the 2-dimensional InAs wetting layer which was not transformed into quantum dots looked responsible for this change in the dot size. However the change rates remained almost same regardless of annealing time and temperature, which may indicate that the morphological change due to thermal annealing is done instantly when the dots are exposed to high temperature annealing.

Quantitative analysis of impurity concentration in purification of Al by segregation method (편석법에 의한 Al정련시 불순물농도의 정량적계산에 관한 연구)

  • Kim, Kyoung-Min;Yoon, Eui-Pak
    • Journal of Korea Foundry Society
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    • v.15 no.5
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    • pp.507-513
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    • 1995
  • The effect of forced convention on the solute redistribution of the Al ingot was studied quantitatively in an effort to fabricate high purity aluminum using a segregation method. Based on the experimental results, the solute concentration in the solid phase tended to decrease at the early state of solidification, and then increased gradually as solidification proceeded. Fe and Si concentrations decreased as growth rate decreased and as revolution speed increased. The solute redistribution obtained from the BPS model incorporated with the tangential flow component as well as the axial flow component within the melt, agreed well with the measurements.

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UBV CCD PHOTOMETRY OF INTERMEDIATE AGE OPEN CLUSTER M11 I. STATISTICAL ANALYSIS

  • SUNG HWANKYUNG;LEE SEE-WOO;LEE MYUNG GYOON;ANN HONG BAE
    • Journal of The Korean Astronomical Society
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    • v.29 no.2
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    • pp.269-278
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    • 1996
  • We present the color-magnitude diagrams (CMD) of more than 24,000 stars in the field of an intermediate age open cluster M11, based on wide field CCD imaging. The morphology of the CMDs varies strikingly as the distance from the center of the cluster increases. From the surface number density analysis, we confirm the mass segregation effect in this cluster: the bright, massive stars are centrally more concetrated than the faint, low mass stars. The slope of the field-corrected surface density with respect to magnitude progressively increases as the radius increases, up to r = 5'. Most of the field stars in or near the cluster main sequence band and in the bright part of the red stars in the CMDs appear to be nearly at the same distance as M11, and they are considered to be the major component of disk stars in the Sagittarius-Carina arm.

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Rational Theories of Discrimination and the Implications for Employment Relations and Transactions (합리적 차별이론과 고용관계 및 거래 관계에 대한 영향)

  • 이세재
    • Journal of Korean Society of Industrial and Systems Engineering
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    • v.24 no.69
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    • pp.36-49
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    • 2001
  • Group level discriminations are observed in employment relations and other transactions in the form of residential occupational and production segregation and differential treatments. Recent developments in the rational theories of discrimination both on the market level and the non-market level are reviewed in terms of their relative strengths, weaknesses and complementarities. Taste discrimination could remain much suppressed in the market but could effect much chain reaction through various ways of statistical discrimination, price discrimination, human capital investment and segregation. Taste discrimination could also take the more structured form of co-workers' requiring compensation for reduced productivity due to increasing interactions with members of different language and culture in a non-segregative system. If could also be viewed in the framework of brand learning models. Non-market models of discrimination are seen to be an essential part to explain extended modes of discrimination.

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Effect of Al-5Ti-B on the Microstructure of Rheology Material (Al-5Ti-B가 레오로지 소재의 미세조직에 미치는 영향)

  • Yang Z.;Seo P. K.;Kang C. G.
    • Proceedings of the Korean Society for Technology of Plasticity Conference
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    • 2005.05a
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    • pp.299-302
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    • 2005
  • Semisolid A356 slurries were prepared by electromagnetic stirring casting and by inoculation of Al-5Ti-B master alloy. As stirring time and addition of Al-5Ti-B are different, the grain size of the primary phase is different. Through the experiment of rheocast in a Buhler horizontal die casting machine, it was found that the finer the equiaxed primary dendrites, the smoother the die filling and better cast quality. Small equiaxed primary dendrite also results in less liquid segregation on the surface.

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