• 한국잡초학회지
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• 제15권2호
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• pp.154-159
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• 1995

야생식물인 마디풀과 버드나무의 수용 추출액에는 상치 및 벼종자의 발아를 강하게 억제하는 물질이 존재하여 이들 품질을 GLC로 검정했다. 마디폴에 페놀물질로는 salicylic + vanillic, tannic - gallic, sinapic, p-coumaric, p-cresol 및 catech이 등의 순으로 함유되어 있었고 이들은 강한 활성을 나타냈다. 배양세포에도 유사한 페놀류가 동정되어 2차 대사물질이 세포배양에도 생성됨이 검정되었다. 지방산 가운데는 linolenic산(3.7 mg/g)과 유기산 가운데는 oxalic산(14.288 mg/g)이 가장 많이 함유되어 있었으며 이들도 생리활성을 지닌 물질로 간주된다. 그 밖에 마디풀에는 석유 에테르 추출물도 1.65% 검정되어 생리활성을 발휘하리라 사료되어 계속 연구중이나 알카로이드 함량은 아주 낮았다. 마디풀과 버드나무로부터 MS기본배지에 2,4-D(1.0 mg/l)과 BAP(0.1-0.2 mg/l)조합 처리하면 캘루스의 유기가 잘 되었으며 대량생산을 위한 세포현탁배양에 이용될 수 있는 기초자료가 된다고 사료된다.

• 식물조직배양학회지
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• 제25권2호
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• pp.103-107
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• 1998

중이온 빔을 이용한 방사선 돌연변이의 기초자료를 얻고자 담배의 수분ㆍ수정 직후의 원배에 100 Gy의 중이온빔을 조사하여 $\textrm{M}_{1}$세대의 종자를 얻었다. NaCl 20과 25%를 포함된 MS배지에 $\textrm{M}_{1}$세대의 종자를 파종하여 17개통의 NaCl저항성 식물체를 선발하였다. 저항성 식물체는 BY-4품종에 $^{14}\textrm{N}$빔을 조사한 처리구에서만 관찰되었다. $\textrm{M}_{1}$세대의 저항성 식물체의 경우 영양생장기간 중에는 외형적 변이가 관찰되지 않았으나 생식생장기의 화기에 있어서는 다양한 변이가 관찰되었다. 특히 암술 수술의 길이의 변화, 꽃잎의 변화, 불완전 화분립, 줄기당 꽃의 착생수 감소 등의 변이가 발생하였다. $\textrm{M}_{1}$세대에서 17개의 NaCl저항성 계통 중에서 8계통은 $\textrm{M}_{2}$ 세대에도 저항성형질이 발현되었고, 또한 $\textrm{M}_{2}$세대의 3계통은 Mannitol이 포함된 배지에서 저항성을 보여주었다.

• Plant Biotechnology Reports
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• 제3권3호
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• pp.191-197
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• 2009

Four-leaf white clover is not found easily in nature due to its low appearance rate (1 in 10,000). Because people believe that it brings good luck and like to either keep it or present it to a loved one, it has commercial and ornamental value. To breed four-leaf clover, we exposed its flowers to ${\gamma}-rays$ at the pollination stage. The $M_1$ seeds produced following doses at 25-100 Gy showed an approximately 74% germination rate, with seedling survival at 46%. In the $M_1$ generation of plants irradiated within that dose range, we found an increased frequency of four leaflets. One of them, Jeju Lucky-1 (JL-1), had a frequency of about 60%. To see whether that mutation was somaclonal or genetic, we observed its $M_2$ generation and found that such a phenotype reappeared. Although our results demonstrated that the irradiation of fully mature flowers led to a higher frequency of 4-leaflets, we could not clearly explain the genetic mechanism involved. We suggest that JL-1 is valuable as a new variety, without further genetic fixation, because white clover can be propagated vegetatively by stolons.

• 한국산림과학회지
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• 제78권2호
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• pp.198-208
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• 1989

버드나무의 잎으로부터 유도(誘導)된 callus의 현탁배양(懸濁培養)은 MS 기본배지(基本培地)에 2,4-D 1.0mg/l와 zeatin 0.1mg/l를 첨가(添加)한 액체배지(液體培地)에서 생장(生長)이 가장 좋았다. 액체배지(液體培地)에서 24일간(日間) 세포(細胞)를 배양(培養)한 후(後) 분리(分離)된 배양액(培養液)은 상치, 벼 그리고 피 종자(種子)의 발아(發芽)를 강(强)하게 억제(抑制)하여 배양중(培養中)에 생리활성(生理活性) 물질(物質)이 형성(形成)되는 것으로 추정(推定)되었다. 배양세포(培養細胞)에서는 pyrogallol, sinapic acid, cinnamic acid, tannic+gallic acid 그리고 P-chlorobenzoic acid 등(等)과 같은 여러 종류(種類)의 phenol 화합물(化合物)이 검출(檢出)되어 이들이 강(强)한 발아(發芽) 억제작용(抑制作用)을 하는 것으로 사료(思料)되었다.

• 한국자원식물학회지
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• 제12권4호
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• pp.260-268
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• 1999

황련(Coptis japonica)의 추출액을 공시하여 Phytophthora capsici, Fusarium oxysporum, Colletotrichum dematium, C. truncatum, Botrytis cinerea, Botryosphaeria dothidea과 Alternaria porri에 대해 항균 활성 검정, 몇가지 작물에 대한 병해 방제 효과를 조사하고 항균 활성 물질을 동정하였다. 황련 추출액은 P. capsici, F. oxysporum, C. dematium, B. cinerea, B. dothidea 및 A. porri에 대해서 항균력이 우수하였다. Methanol을 용매로 추출하여 분리된 물질과 berberine-Cl 표준품의 항균활성을 비교할 때, 황련추출액으로부터 분리된 물질은 표준품 berberine-Cl과 비슷하게 항균력을 나타내었다. 황련 추출액은 고추 역병(83.3%), 참깨 모잘록병 (92.0%) 및 파 검은무늬병(87.5%)에 대해서 방제효과가 인정되었다. 고추 잎과 과실, 파 잎 및 딸기 과실에는 약해가 나타나지 않았으나 고농도에서는 고추 및 참깨의 발아를 억제하거나 유근의 신장을 억제하는 것으로 나타났다. Methanol로 추출한 황련 추출액으로부터 황색 분말 4.24g/100g을 얻을 수 있었다. 이들 물질을 HPLC로 분석한 결과 항균성을 나타내는 물질은 berberine-Cl로 동정되었다.

• Journal of Plant Biology
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• 제20권1호
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• pp.7-14
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• 1977

The seeds of bean(Glycine max M.) and corn(Zea mays L.) soaked in 5000ppm lead solution for 24 hours were sowed in the flowerpots being placed sandy-clay soil under the field condition. The fixed concentrations of various anions and 2000 ppm lead were supplied alternately in the sandy-clay soi of the flowerpots at two days interval from May to July in 1976. After the plants were harvested prior to the flowering stage, the lead contents of plnat and soil were analyzed by atomic absorption spectrophotometer. The lead contents absorbed by the plant roots showed the highest in an weak acid soil of the best suitable condition of plant growth. The absorption of lead by the plant roots was inhibited by the various anions, especially divalent anions of the soil. Some phosphate anions inhibited lead absorption more than otehr various anions in the soil. The more various anions were in the soil, the more plants could be protected from the lead toxicity. In the case of lead supply in the soil, 99.5% of lead was accumulated in the upper layer of the soil(0-10cm), and 0.5% of lead accumulated in the lower layer (10-20cm). Therefore, the yellow-brown and white symptoms on the leaves and the inhibition of root growth by lead toxicity was increased in the early stage of the germination, however decreased in accordance with the progress of the growing stage becuase of the root growth toward beneath the lower layer of the soil. In spite of the contents of 3773ppm lead in the soil, the symptoms of lead toxicity was not found in the grown plants. At that time the lead contents of the plants absorbed from the soil were minimum 0.78ppm and maximum 3.64ppm through the growing stage.

• Journal of Forest and Environmental Science
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• 제37권4호
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• pp.323-330
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• 2021

Zygotic embryo culture was performed to propagate evergreen oak, Quercus myrsinifolia, which has recalcitrant seeds and is difficult to propagate by cuttings. Zygotic embryos appeared in WPM medium after 14 days, and after 56 days, they developed into complete plants with cotyledons and roots. The medium suitable for zygotic embryo culture was 1/4 WPM medium, showing a shoot growth of 2.43 cm and root growth of 8.7 cm after 8 weeks of culture. As a result of investigating the effect of GA₃ on the growth of plants germinated from zygotic embryos through GA₃ treatment, the best growth was shown in 0.5 mg/l GA₃ treatment. The in vitro rooting and growth of IBA-treated zygotic embryo-derived plants were good in the 0.5 mg/l IBA treatment and rooting and shoot growth were not observed at higher concentrations. And the callus induction rate also increased as the concentration of IBA increased. Plants grown in vitro were transferred to a plastic pot containing artificial soil and acclimatized in a greenhouse for about 4 weeks, resulting in more than 90% survival. As a result of this study, the zygotic embryo culture method was confirmed to be effective for mass propagation of Q. myrsinifolia. The results of this study are expected to contribute significantly to the mass propagation of elite Q. myrsinifolia.

• 한국초지조사료학회지
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• 제42권3호
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• pp.146-154
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• 2022

This experiment was conducted to evaluate the growth characteristics and productivity of silage corn varieties developed in Korea. Corn cultivation was carried out using the experimental field in the Pyeongchang campus of Seoul National University (550 m above sea level). There have 10 domestic cultivars (Gwangpyeongok, Dacheongok, Yanganok, Jangdaok, Cheongdaok, Daanok, Sinhwangok, Sinhwangok II, Pyeonggangok, and Hwangdaok) with one imported cultivar (P1543) which tested as a control, and randomized block design with three replications. Among the 100-grains weight of the seeds, Dacheongok was the heaviest, and the germination rate for each variety was 74.6% on average, while that of Daanok and Sinhwangok were over 90%. Sinhwangok was the fastest in tasseling and silking date. The number of days required to be silking date was as slow as 85 days in Dacheongok, Cheongdaok and Pyeonggangok, and as fast as 80 days or less in Sinhwangok, Sinhwangok II and Hwangdaok. The plant height of P1543 was the highest as 344cm, and Hwangdaok and Daanok were short. In terms of the ratio of ears, Daanok had the highest rate of 60.18%, and Jangdaok and Dacheongok had the lowest. There was no significant difference in dry matter content in stover, but P1543 was generally higher in ear and total dry matter content. The dry matter yield was highest in P1543, and the yield of TDN was significantly higher in P1543 and Yanganok. There was a significant difference in the crude protein content of ears and the dry digestibility of stems (p<0.05), while there was no significant difference in the content of each part or element. Combining the above results, Yanganok was the highest in terms of yield, and Dacheongok, Sinhwangok and Pyeonggangok were also recommended for domestically grown corn varieties in the mountainous regions of Gangwon-do.

• 한국자원식물학회지
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• 제35권4호
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• pp.508-514
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• 2022

Bulbophyllum auricomum Lindl. is very popular among orchid growers due to the attractive fragrance of its flowers and has become an endangered orchid in Myanmar. In this study, we carried out an aseptic technique that can be used to conserve this endangered orchid species. The seeds of B. auricomum Lindl. were obtained from artificial pollination and cultured in MS basal medium for seed germination. The effect of coconut water and BAP in MS basal medium on callus induction was investigated. The highest callus induction was found at 2.0 mg/L BAP. The maximum growth of protocorm-like bodies (PLBs) was evaluated, and the best response was observed on MS medium supplemented with 150 mL/L coconut water at pH 5.6. MS basal medium supplemented with 150 mL/L coconut water along with 2.0 mg/L BAP and 1.0 mg/L NAA (MCBN) showed the highest number of plantlets at 15℃ at the second week of culture. At the second and third week of culture, MS medium supplemented with 2.0 mg/L BAP and 1.0 mg/L NAA (MBN) showed the best result in terms of the number of leaves and the longest leaves at 15℃ and 25℃, respectively. The present study showed evidence of successful in vitro propagation of B. auricomum Lindl.

• 생태와환경
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• 제55권4호
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• pp.406-414
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• 2022

Bolboschoenus planiculmis has been acknowledged as a key species in whooper swans (Cygnus cygnus) habitat by providing food for this migratory waterfowl. B. planiculmis wetlands are being degraded by water shortages and salinization caused by anthropogenic activities and climate changes. In 2004, the distribution of B. planiculmis in the tidal flats of the Nakdong Estuary was 2,475,568 m², and in 2021, the distribution area was 798,731 m², which decreased by 32.3%. In order to restore the degraded B. planiculmis wetlands, shoot transplantation and seed sowing were tentatively used in three places with different salinity and water levels. The average density per unit area in September at the optimal growth period after planting were A (fresh water level 50 cm) 58±15.65 m^-2, B (brackish water level 0~5 cm) 188±63.83 m^-2, C (brackish water level 0 cm or less) 188±45.13 m^-2. The tubers were observed as A 0 g dw m^-2, B 25.32±2.94 g dw m^-2, and C 13.39±0.91 g dw m^-2. Tubers were distributed in the soil, with only 3.0% at the 10~20 cm depth but 97.0% at the 0~10 cm depth. In contrast, the germination rate of B. planiculmis seeds was observed to be 0%. Results of this study provide technical support for the restoration of B. planiculmis wetland and the improvement in the quality of whooper swans habitat.