• Journal of Plant Biology
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• v.13 no.4
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• pp.23-31
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• 1970

The rice variety, Kwanok, was reared in the water and land beds and transplanted to the reclaimed soil area, having an average salt concentration of 0.39%. Two levels of the moderate and late season cultures with 4 treatments were used. The K and Si contents of the stem part of land bed seedlings were somewhat smaller, but total carbohydrate remarkably larger, the C/N ratio was accordingly greater than water bed seedlings. The rooting ability of land bed seedlings was vigorous markedly in culture solutions, to which added various concentrations of NaCl, The rooting ability of each seedling water not much declined in theculturing solution of up to 9.4mmhos/cm, (0.6%) of salt concentration, but it was drastically declined in the salt concentration over that. It seemed that the critical salt concentration for the rooting rice plant. The land bed seedlings in each salty condition markedly decreased compared with the water bed seeldings in transpiration rate and it showed a stronger drought resistance and contained a large amount of chlorophyll at transplanting stage, and also showed higher stability of chlorophyll at rooting stage in the salt treatment. The number of panicles, panicle weight, number of grains per panicle and ratio of matured grains of the rice plant grown by the land bed seedlings were much greater and 1,000 grain weight was less than from water bed seedlings. The cultural practices with the land bed seedlings increased the rough rice yields by 15% and 11%, respectively, compared with the yields of the moderate and late season cultures by water bed seedlings.

• Journal of Ecology and Environment
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• v.32 no.3
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• pp.149-158
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• 2009

American sycamore seedlings were grown in chambers with two different ozone concentrations ($O_3$-free air and air with additional $O_3$) for 45 days. Both the control and the $O_3$ chambers included non-fertilized and fertilized plants. After 18 days of $O_3$ fumigation, seedlings were placed in a clean chamber for 27 days. Seedlings under ozone fumigation showed a significant decrease in pigment contents and photosynthetic activity, and a significant increase in lipid peroxidation. Fertilization enhanced physiological damage such as the inhibition of photosynthetic activity and the increase of lipid peroxidation under ozone fumigation. During the recovery phase, the physiological damage level of seedlings increased with ozone fumigation. In addition, physiological damage was observed in the fertilized seedlings. Superoxide dismutase (SOD) and glutathione reductase (GR) activities of $O_3$-treated seedlings increased up to 33.8% and 16.3% in the fertilized plants. The increase of SOD activity was higher in the fertilized plants than in the non-fertilized plants. Negative effects of ozone treatment were observed in the biomass of the leaves and the total dry weight of the fertilized sycamore seedlings. The $O_3$-treated seedlings decreased in stem, root and total dry weight, and the loss of biomass was statistically significant in the fertilized plants. In conclusion, physiological disturbance under normal nutrient conditions has an effect on growth response. In contrast, in conditions of energy shortage, although stress represents a physiological inhibition, it does not seem to affect the growth response.

• Korean Journal of Soil Science and Fertilizer
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• v.49 no.1
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• pp.44-52
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• 2016

Plants response to phosphate starvation include the changes of activity of some enzymes, such as phosphatases, fructose-1,6-bisphosphatase, sucrose-phosphate synthase and nitrate reductase. In this study, to determine the effects of phosphate starvation on the change of activities of acid and alkaline phosphatase, fructose-1,6-bisphosphatase, sucrose-phosphate synthase, and nitrate reductase were studied in melon seedlings (Cucumis melo L.). The content of the protein and chlorophyll tended to relatively reduced in melon seedlings subjected to phosphate starvation. Acid phosphatase activity in first and second leaves of melon seedlings was relatively higher than that of third and fourth leaves of seedlings in 14 days after phosphate starvation treatment, respectively. Active native-PAGE band patterns of acid phosphatase in melon leaves showed similar to activities of acid phosphatase, whereas alkaline phosphatase activity was different from the change in the activity of acid phosphatase. Inorganic phosphate content in melon seedlings leaves was constant. The changes of Fructose-1,6-bisphosphatase and sucrose phosphate synthase activities showed similar patterns in melon seedlings leaves, and between these enzymes activities and phosphate nutrition negatively related. Fructose-1,6- bisphosphatase and sucrose phosphate synthase activities showed significant difference in second and fourth leaves, but nitrate reductase showed significant difference in first and second leaves in 14days after phosphate starvation treatment. We concluded that phosphate nutrition could affect the distribution of phosphate, carbon and nitrogen in melon seedlings.

• Journal of Plant Biotechnology
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• v.32 no.4
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• pp.275-279
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• 2005

Tah tasai chinese cabbage (Brassica campestris var. narinosa), a vegetable plant popularly consumed as several-days-old seedlings in oriental countries, can be easily cultivated using a simple appliance. We demonstrated that Agrobacterium-mediated transformation via vacuum infiltration (agroinfiltration) resulted in a successful transient GUS gene expression in tah tasai chinese cabbage seedlings. Pre-germinated seeds were found to be more susceptible to Agrobacterium infection than one-day-old or two-days-old seedlings. We also demonstrated that hydrogen peroxide (HPO) treatment increased GUS expression especially for two-days-old seedlings. In ELISA using seedlings transformed with hepatitis B surface antigen (HBsAg) DNA by agroinfiltration, HBsAg protein synthesis increased more than two folds by HPO treatment to two-days-old seedlings in comparison to the mock-treated pre-germinated seeds.

• Korean Journal of Organic Agriculture
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• v.8 no.1
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• pp.89-99
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• 1999

To do mass multiplication of plug seedlings in potatoes, apical stem cuttings originated from virus-free microtubers were cut to one-two internodes and transplanted into the plug-tray. After 10days, we applied Chitosan and Wood Extraction on rooting and tuber formation of plug seedlings. To improve field adaptability of plug seedlings, rooted cuttings with a height of 20cm after 20days of cutting were transplanted ito the fields, We applied 500~2000ppm Chitosan on growth characteristics and tuber formation of that. The above and underground growths, such as plant height and number of leaves were significantly more vigorous after treatment with 500~1,000ppm Chitosan and 2,000ppm Wood Extraction, the spray treatment was carried out five times at intervals of four days after ten days of transplanting. T-N, K, P, Mg and Na, were higher as the concentrations of chitosan and Wood Extraction were higher. The growth and tuber yield in plug seedlings planting plot and seed potatoes planting plot were effectively highter as foliar application of Chitosan(500~2,000ppm) was done after planting the plot. T-N content in leaves and tuber was higher as the concentration of Chitosan was high. A similar tendency was shown in K, P and Mg. In the small tuber(under 30g), the number of tubers and tuber yield were relatively increased in the seed potatoes planting plot, but the large tubers(over 80g) yield was higher in the plug seedlings planting plot, and in order to increase tuber yield in plug seedlings it was necessary to add plant density to the field.

• Current Research on Agriculture and Life Sciences
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• v.23
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• pp.1-8
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• 2005

The purpose of this study was carried out to determine the optimum sowing date and days after sowing (DAS) in automatic facility located at Uiseong of Gyeoungbuk province. The seedling height was rapidly increased with a delay of sowing time from April to May. The 10-day old seedlings with suitable plant height (> l0cm) for machine transplanting in the facility were obtained when the seeds sowed after May 20 at Uiseong in Gyeoungbuk province. The dry weight-height ratio of 10-day old seedlings was significantly decreased when the seeds sowed after June 20, and 15-days old seedlings did after May 10. No. of panicles/hill was reduced with increasing of days after seeding. But there were no significant difference among other yield components such as no. of grains/panicle, ratio of ripened grains, and 1,000 grain weight. The highest grain yield, 748kg/10a, was attained from the 10-day old seedlings transplanted at May 27 in comparision with those of 15-day and 20-day old seedlings. The grain yield of 10-day old seedlings transplanted at June 26 was also higher than that of 15-day old seedlings.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.16 no.3
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• pp.97-105
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• 2013

Kalopanax septemlobus (Thunb. ex Murray) Koidz. is natively distributed in Korea. The importance of this species has been increased not only for high quality timber but for medicinal and edible uses. However, increasing market demand of K. septemlobus with illegal cutting and overexploitation has resulted in its rapid depletion and destruction of natural habitat. This study was conducted to understand the survival rate and growth characteristics of planted K. septemlobus seedlings with treatment of root pruning, stem cutting and planting density. The survival rate and growth of height and root-collar diameter for one- and two-year old seedlings with different planting densities were investigated in the clear-cut area of a Pinus densiflora stand for five years. One-year-old seedlings were treated with or without root pruning and planted with three density levels (5,000 trees $ha^{-1}$, 10,000 trees $ha^{-1}$, and 40,000 trees $ha^{-1}$). Two-year-old seedlings were treated with and without stem cutting and planted with the density of 5,000 trees $ha^{-1}$. The survival rate of one-year-old seedlings with root pruning treatment in the density of 10,000 trees $ha^{-1}$ was 92%, while that without root pruning in the density of 40,000 trees $ha^{-1}$ was 67% after five years. The height of one-year-old seedlings has been significantly affected only by planting density in the $5^{th}$ year. The survival rate of the two-year-old seedlings with stem cutting was 75.5% and greater than control (67.3%) in the $5^{th}$ year but no difference in height was shown between the two treatments from three years after plantation.

• Journal of Biosystems Engineering
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• v.4 no.1
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• pp.48-57
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• 1979

In order to obtain a standard reference for designing an adequate power rice transplanter, the cutting forces depending upon variety of seedling, sowing density, seedling age and soil moisture content of mat-type seedling were measured by the rice transplanter installed with force measuring device of dynamic strain gage system in the laboratory. The result of this study are summarized as follows : 1. Cutting velocity and acceleration transplanting hoe obtained from jinematic analysis of planting mechanism was 1.32m/sec and 81.5m/$sec^2$ when planting crank-shaft rpm was 160. 2. Little difference between cutting forces on 30-days old seelings of japonica and Indica type was observed, as the cutting forces determined were 2.0kg per hill for Japonica type and 2.1kg per hill for Indica type. 3. Cutting forces determined on 40-days old seedlings were 2.5kg, 2.3kg, 3.1kg and 2.9kg per hill for Milyang No.15, Tongil, Akibare and Milyang No.23 compared to the other varieties. 4. The cutting force was not greatly affected by the sowing densities , only five percent of differences were observed epending upon the sowing densities. 5. Cutting forces were 2.7kg and 2.0kg per hill on 40-days old seedlings and 30-days old seedlings respectively. About 38 percent of more forces was required in cutting 40-days old seedling than in cutting 30-days old seedlings. 6. More cutting forces were required as soil moisture content of mat-type seedling was decreased. 7. Root length after cutting by the planting hoe and their relationships with soil moisture content on 30-days old seedlings, are as follows ; $y=4.147-11.384x+ 28.854x^2$ where , $y$=root length after cutting. (cm) , $x$=soil ture content of mat type seedlings.(%, d.b.) 8. Cutting forces were varied with the width of cuttings ; those on 40-days old mat type seedlings were 2.7kg and 2.2kg per hill when cutting with 14 mm and 10mm of width respectively, about 32 percent of more forces was required when cuting with 14mm of width compared to 10mm of width.

• Mycobiology
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• v.42 no.4
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• pp.331-338
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• 2014

To examine the effects of water stress and Cenangium ferruginosum (CF) on the fungal endophytic community of needles of Pinus koraiensis (PK), fungal endophytes isolated from the needles of 5-year-old PK seedlings were compared before and after exposure to water stress conditions and artificial inoculation with CF ascospores. Artificial CF inoculation was successfully confirmed using PCR with CF-specific primers (CfF and CfR). For comparison of the degree of water deficit in water-stressed and control groups of PK seedlings infected with CF, the water saturation deficit and water potential were measured. Lower water potential estimates were found in the water-stressed seedlings than in the control group. The fungal endophytes isolated from the second-year needles of non-water-stressed seedlings before and after CF inoculation revealed that primary saprobes were approximately 30% and 71.7%, respectively, and the remaining endophytes were rot fungi or pathogens. Sixty days after CF inoculation, diverse fungal endophytes in the first-year needles were isolated from the water-stressed seedlings. However, some fungal endophytes isolated from the non-water-stressed seedlings were also identified. Fungal endophytes in the second-year needles of the water-stressed and non-water-stressed seedlings were approximately 8% and 71.7% of saprobes, respectively, and the remaining endophytes were rot fungi or pathogens. On the basis of the results, we conclude that water deficit and CF can have an effect on fungal endophytic communities in the needles of PK seedlings.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.23 no.4
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• pp.59-67
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• 2020

In this study, the effects of windbreak fences composed of natural vegetation on one-year-old seedlings were analyzed to develop restoration methods for an endangered subalpine species, the dwarf Siberian pine (Pinus pumila (Pall.) Regel). One-year-old seedlings were planted in 2016 by sowing seeds that had been collected from the Daecheongbong area on Mt. Seoraksan, South Korea, in 2014. The area near Daecheongbong was selected as the experimental site, and treatment and control plots (2m×2m) were installed at the site. To analyze the effects of wind protection, windbreak fences were constructed in the treatment plots using hairy Korean rhododendrons (Rhododendron mucronulatum Turcz. var. ciliatum Nakai) from the surrounding area and weather stations were installed to investigate atmospheric temperature, humidity, and wind speed. In all control plots without windbreak fences, dwarf Siberian pine seedlings were killed by strong winds seven months after planting. In contrast, the average survival rate of the seedlings in treatment plots was 96.7% after seven months, 64.2% after two years, and 45% after three years, with most (85.3%) of the seedlings showing good initial root establishment. Accordingly, windbreak fences composed of natural vegetation are suitable for promoting the early establishment of seedlings in the restoration of dwarf Siberian pine stands.