• Research in Plant Disease
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• v.11 no.2
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• pp.106-114
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• 2005

To breed powdery mildew resistant varieties, 104 accessions of Cucurbita spp. germplasm were evaluated. Powdery mildew resistance and horticultural characteristics of the accessions were investigated in the field conditions. C. martinezii was selected for high resistance to powdery mildew in the field. The growth of powdery mildew pathogen and response of leaf surface tissue to the pathogen were observed after artificial inoculation to resistant C martinezii and susceptible C. moschata cv. 'Wonye No.402'. Mycelium growth was very clearly different between resistant C. martinezii and susceptible C moschata cv. 'Wonye No. 402' at 5 days after inoculation at the adult stage. Interspecific hybridization was attempted to transfer powdery mildew resistance gene(s) from C. martinezii to C. moschata. A single dominant gene action was postulated as deter mining powdery mildew resistance at the seedling stage. But for the effective selection of powdery mildew resistant individuals, it was established that adult plant resistance should be confirmed in the field conditions after the seedling test. Through the seedling and field tests, C. moschata lines resistant to powdery mildew and superior to horticultural characteristics, were selected after 2 backcrossing and 3 selfing generations. The fruit characteristics of newly developed lines were similar to those of the recurrent parent, 'Jaechenjarae'.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.42 no.6
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• pp.821-832
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• 1997

The aim of this study was to separate or purify some bioactive compounds from flowers of alfalfa(Medicago sativa L.) and to test of the isolated compounds on alfalfa for their autotoxicity and on Echinochloa crus-galli for their allelopathy for seed germination and seedling weight. Using thin layer chromatography(TLC) of $CHCl_3$ extracts, the most inhibitory band to alfalfa seed germination was determined. Germination inhibition of this extract suggested a complex chemical interaction. Separation and purification of compounds with CHCl$_3$ extract of fresh alfalfa flowers were conducted by a silica gel TLC, and microcrystalline cellulose TLC(MCTLC), followed by droplet countercurrent chromatography(DCCC) bioassay. Preliminary identification was done by high perfomance liquid chromatography(HPLC) on the most inhibitory fractions in DCCC. Ferulic acid, caffeic acid, vanillic acid, rutin, narringin were identified in fraction 5 and ferulic acid, caffeic acid, vanillic acid, rutin, coumarin in fraction 6. The phytotoxicity of their individual compound was tested on alfalfa and Echinochloa crus-galli seed germination and seedling weight. Coumarin and ferulic acid showed the most inhibitory effect on alfalfa seed germination and Echinochloa crus-galli seedling fresh and dry weight. These compounds may be, at least in part, involved in autotoxicity and allelopathy.

• Journal of Environmental Science International
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• v.30 no.8
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• pp.685-691
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• 2021

This study aimed to find a suitable size and a seedling raising stage for growing cuttings of Euonymus fortunei 'Emerald and Gold' using plug trays. The experimental method, involved cutting two nodes from a solitary branch of E. fortunei 'Emerald and Gold', and the use of 32 (143 mL/cell), 50 (70 mL/cell), 105 (18 mL/cell), 200 (13 mL/cell) plug trays. The cuttings were transplanted to trays after they were filled with a universal horticultural medium. To compare the growths, plant heights, the numbers of leaves, longest root lengths, thickness/radius ratios, dry weights, and fresh weights were measured from July to October, and statistical analyses were performed using both the two-way repeated-measures analysis of variance (ANOVA) and Tukey's post-test. The results confirmed that the size of the plug tray and the seedling raising stage had a significant effect on the growth of E. fortunei 'Emerald and Gold.' In addition, the overall growth was high and the change in growth was relatively rapid in districts 50 and 105. Therefore, it can be considered appropriate to use 50 and 105 trays when growing cuttings of E. fortunei 'Emerald and Gold' on plug trays.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.36 no.1
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• pp.65-69
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• 1991

In order for investigating the factors related to decrease of seedling vigor in the naked barley lines, two naked lines and a covered variety were sprouted with 20 mm rainfall in the natural condition, dried and the sprouted seeds were threshed by hand and small machine thresher, Chiyota. In the case of hand-threshing of the sprouted seeds, the germination rate index (GRI), germination rate, and TTC test value of the different varieties were not different due to the different sprouting degrees, but emergence rate index (ERI) became decreased with stronger sprouting damage. In comparison with hand-threshing, the embryos of sprouted seeds from machine threshing with 700 rpm were partially damaged, and the traits related to seedling vigor were decreased highly significantly. However, the diastatic activity was sharply increased by stronger sprouting and machine threshing. The sprouting degree in the hand-threshing plot was significantly correlated with the diastatic activity(r=0.931＊＊, 0.951＊＊) and GRI(r=0.461), respectively.

• Journal of The Korean Society of Agricultural Engineers
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• v.65 no.2
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• pp.73-80
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• 2023

This study was conducted to analyze changes of irrigation water and soil volumetric water content by irrigation method of field soil in Saemangeum reclaimed tideland. The main test irrigation methods was surface drip irrigation, sprinkler irrigation, and sub drip irrigation. In addition, the correlation between irrigation amounts and crop yield by irrigation method was investigated. For soil volumetric water contents increases by 25%, surface drip irrigation took 1.5 hour, sprinkler irrigation took 2.0 hours, and sub drip irrigation took 3.0 hours. As a result of analyzing the irrigation amounts according to the yield, the surface drip irrigation was 2.66 mm/day in the seedling stages, 3.31 mm/day in the vegetative growth stages, and 5.09 mm/day in the flowering stages. Sprinkler irrigation was 2.90 mm/day in the seedling stages, 3.87 mm/day in the vegetative growth stages, and 7.11 mm/day in the flowering stages. Sub drip irrigation was 2.42 mm/day in the seedling stages, 3.09 mm/day in the vegetative growth stages, and 4.87 mm/day in the flowering stages. It was analyzed that there was a statistically significant difference in irrigation amounts by fresh weight and irrigation method (F=4.002, p=0.022), and irrigation amounts by dry weight and irrigation method (F=3.499 p=0.034). Surface drip irrigation was judged to be more appropriate than sprinkler irrigation or sub drip irrigation for field crops in Saemangeum reclaimed land.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.59 no.3
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• pp.341-349
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• 2014

Field emergence of Azuki bean is poor due to hard seed coat as compared to other legumes. In this study, an attempt was made to develop prediction method with regression analysis based on various seed vigor tests in laboratory for field emergence of azuki bean. Azuki bean seeds artificially aged to provide various levels of seed quality were evaluated by the standard germination test (SGT), cold germination test (CT), cool germination test (CGT), complex stressing vigor test (CSVT), tetrazolium(TZ) vigor test and electroconductivity test. The SGT was suitable for predicting the field emergence in the unaged high vigor seeds. The abnormal seedling percentage and shoot length in the CGT were highly correlated with field emergence of moderate vigor seeds artificially aged for 2 days. Electroconductivity, seed viability in the CSVT, and vigor and predicted germinability in the tetrazolium vigor test were also useful for predicting field emergence. Percent of ungerminated seed in the CSVT was correlated with field emergence in the low vigor seeds artificially aged for 4 days. In a stepwise multiple regression analysis, seed viability in the SGT, normal seedling percentage and dry matter weight in the CGT accounted for 86.9% of the predicted value of field emergence in azuki bean.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.34 no.2
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• pp.134-141
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• 1989

In order to estimate field seedling emergence and grain yield by the information collected from various laboratory seed vigor tests, each two malting and naked barley cultivar seeds were artificially aged according to the standard aging treatment suggested by the Association of Official Seed Analysts. The seed vigor tests adopted were warm- and cold-germination test, tetrazolium vigor test and electrocon. ductivity test. Field emergence of malting barley (Y) was estimated by Y=-2.962+0.229X$_1$ (% warm germination) -0.001X$_2$ (vigor of warm germination test: WGT) +0.354X$_3$ (vigor of cold germination test: CT) -0.558X$_4$ (% cold germination). The multiple correlation coefficient indicated that % warm germination. was contributed 64 % of the variation in seedling emergence rate of malting barley. The vigor of warm and cold germination tests, and % cold germination contributed additional 4, 7, and 9%, respectively, upon addition of the variables into regression. For naked barley, the regression equation of emergence rate was less efficient(R$^2$=54%) than that of malting barley(R$^2$=84%). A model to predict grain yield by the results of various seed vigor tests was not evaluated for both malting and naked barlev.

• Proceedings of the Korean Society for Agricultural Machinery Conference
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• 1993.10a
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• pp.256-266
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• 1993

History , achievement and future of direct-wet-rice-seeding technique in Korea and problems encountered in on-farming research were introduced. Some farmers are very eager to lower production cost and save labor. Their effort results in several direct-wet-seeding methods and seeding machines. The design problems and improvement points related to the direct -wet-rice-seeding machines were drawn out of field test.

• Journal of Plant Biology
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• v.17 no.1
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• pp.9-14
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• 1974

Inhibitory effect of alantolactone and isoalantolactone was shwon in Avena straight growth test and in the formation of adventitious root in Phaseolus seedling. However, di-, and tetrahydroalantolactones given no effect on the elongation and the rooting. Inhibitory effect of alantolactone could partly be removed by cysteine, cystine, and reduced glutthione. The plant materials were made less sensitive to alantolactone by the pretreatment of cysteine, but cysteine supplied after the treatment of alantolactone brought about no effect on the action of alantolactone. A new spot was shown on TLC plate from the mixture of alantolactone and cysteine, indicating that alantolactone can be inactivated by cysteine, not cystine, without any biological processes.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.43 no.4
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• pp.234-238
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• 1998

To select new germplasm for salinity tolerance from new plant type (NPT) breeding lines, the sixty F$_4$ lines selected from the crosses between Korean cultivars and IRRI's NPT lines were evaluated for salinity tolerance at the seedling stage with salinized culture solution (EC=12 dS/m) in the controlled conditions. Two NTP lines derived from a cross between 'Ilmibyeo' and 'IR66152-AC5-1', 'HR15258-7-1' and 'HR15258-27-1', were found to have good tolerance. The salinity tolerance of the lines was compared to their parents and the sensitive ('IR29') and tolerant ('Pokkali') checks in three salinity levels, no salinity (control) and an EC of 12 and 16 dS/m. Visual salinity score, shoot Na+ and Na-K ratio in two NPT lines was significantly low compared with the parents and IR29. Indicating that salinity tolerance of the lines might be derived from a transgressive segregation. The relative water content of the lines was higher than Pokkali, and the dry weight of shoot and root was proportionally decreased to salinity score and salinizing concentration. The visual salinity scores were significantly correlated with shoot Na concentration, Na-K ratio, relative water content, and reduction of dry weight (P<0.01). Their tolerance was attributed to root and shoot characteristics that led to high shoot water content, thus diluting the toxic effect of salts.