• Title/Summary/Keyword: Seedling test

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Effect of Methylotrophic Bacteria in Seedling Development of Some Crops under Gnotobiotic Condition (Methylotrophic bacteria 접종이 작물 유묘 생장에 미치는 영향)

  • Hong, In-Soo;Kim, Jun-Seok;Lee, Min-Kyoung;Yim, Woo-Jong;Islam, Md. Rashedul;Boruah, Hari P. Deka;Chauhan, Puneet Singh;Han, Gwang-Hyun;Sa, Tong-Min
    • Korean Journal of Soil Science and Fertilizer
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    • v.42 no.4
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    • pp.317-322
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    • 2009
  • Healthy seedling generation is the major concern in overcoming adverse effects of biotic and abiotic stresses during tender stage of development in vegetables and horticultural crops. Because of this, priority is given to research leading to the generation of healthy seedlings in crops subjected to transplanting and bedding. In this study, growth pouch experiments were conducted to determine the effect of inoculation of six different strains of Methylobacterium sp. namely, M. oryzae CBMB20, M. phyllosphaerae CBMB27, M. suomiense CBMB120, and Methylobacterium strains CBMB12, CBMB15 and CBMB17 on the seedling development of the vegetable crops cabbage, Chinese cabbage and cucumber; and horticultural crops tomato and red pepper. Crops treated with the test strains generally showed higher seedling dry matter accumulation compared to the control. Significantly higher accumulation was exhibited by CBMB12, CBMB17, and CBMB20 in cabbage, as well as for CBMB27 and CBMB120 on tomato and Chinese cabbage, respectively. Furthermore, all the strains promoted root elongation in cucumber and tomato seedlings while in Chinese cabbage and red pepper, root elongation was observed with CBMB120 and CBMB12 inoculation, respectively. Large scale nursery study is needed to develop a thorough protocol for healthy seedling development with the use of these strains.

Allelopathic Effect of Some Weed Species Extracts and Residues on Alfalfa (몇가지 잡초들의 추출물과 잔유물의 알팔파에 대한 타감작용)

  • III-Min Chung;Ki-June Kim;Kwang-Ho Kim;Joung-Kuk Ahn
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.39 no.3
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    • pp.285-294
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    • 1994
  • Dried top and root extracts of seven different weed species, fresh top and root extracts, and various concentrations of extract (0, 5, 10 and 15%, W/V) and residue rate (0.0, 0.25. 0.5, 0.75 and 1.0%) of velvetleaf (Abutilon theophrasti Medic.) were used to study their allelopathic effects on alfalfa in the laboratory and greenhouse. Top and root aqueous extracts of common lambsquarter (Chenopodium album L.), giant foxtail (Setaria faberii Herrm), redroot pig-weed (Amaranthus retroflexus L), velvetleaf, large crabgrass (Digitaria sanguinalis L), canada thistle (Cirsium arvense L.) and prostrate knotweed (Polygonium aviculare L.) significantly inhibited germination, seedling length, weight, and vigor in alfalfa. Top growth extracts of weeds exhibited greater allelopathic effects than root extracts. Alfalfa test species, WL-320, responded significantly different to the various weed species extracts in terms of allelopathic effect. The regression slopes of various top extracts showed significant variation with respect to germination percentage. Velvetleaf (b=3.69) extracts were the most inhibitory, while large crabgrass (b=2.39) extracts had the least allelopathic effect on alfalfa. When compared the activity of fresh velvetleaf extract to that of dry velvetleaf extract, dry extract was more inhibitory to alfalfa germination and seedling growth. Germination, seedling length and weight of alfalfa were inversely proportional to the concentration of dried velvetleaf extracts. Seedling emergence and survival percentage was inhibited by velvetleaf residue mixture treatment. Also, more of the toxic effects were observed from the dried top extracts, as compared to extracts from fresh top and root. These results demonstrate the allelopathic activity of different weed species extracts and suggest that weeds may affect crop growth and development due to the inhibitory effects of allelochemicals present in weed tissue.

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Assessment of Allelopathic Potential of Some Weed Species on Alfalfa(Medicago sativa L.) Germination and Early Seedling Growth (알팔파 발아와 초기생육에 대한 잡초종의 Allelopathic 잠재성 평가)

  • Chung, I.M.;Miller, D.A.
    • Korean Journal of Weed Science
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    • v.15 no.2
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    • pp.121-130
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    • 1995
  • Greenhouse and laboratory studies were conducted to investigate allelopathic potential of some weed species on alfalfa(Medicago sativa L.) germination and seedling growth. In the comparison between top(leaves+stems) and root extracts, top extract exhibited greater allelopathic effects on alfalfa germination than that of root. The various weed species extract differently responded to alfalfa test species, WL-320, in terms of allelopathic effect. Top and root aqueous extracts of lambsquarter(Chenopodium album L.), giant foxtail(Setaria faberii Herrm.), redroot pigweed (Amaranthus retroflexus L.), velvetleaf(Abutilon theophrasti Medic.), crabgrass(Digitaria sanguinalis L.), canada thistle(Cirsium arvense L.) and prostrate knotweed(Polygonium aviculare L.) significantly inhibited germination, seedling length, weight, vigor, and rate of germination of alfalfa. The regression slopes of various top extracts showed that velvetleaf(b=3.69) extracts were the most inhibitory, while large crabgrass(b=2.39) extracts had the least allelopathic effect on alfalfa germination. Germination, seedling length and weight of alfalfa were inversely proportional to the concentration of dried velvetleaf extracts. Also, more of the toxic effects were observed from the dried extracts compared to the fresh extracts. Residue of velvetleaf inhibited significantly alfalfa emergence and survival percentage compared to the control. The emergence and survival percentage of alfalfa were 44%, 57% at 1.0% residue treatment, respectively. When weed residues were mixed with silica sand with incubation time, velvetleaf residue most inhibited alfalfa growth. The degree of inhibition increased as incubation time increased. An incubation for 72h caused the greatest inhibition of alfalfa growth. These results demonstrate the different allelopathic activity of weed species extracts on alfalfa and suggest that weed may affect alfalfa growth and development through the inhibitory effects of allelochemicals present in weed tissue.

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Studies on the Physical Properties of Base Paper for the Manufacture of Mulching Mat for Afforestation Seedling (조림묘목 mulching mat 제조용 base paper의 물리적 특성 연구)

  • Kim, Hyoung-Jin;Oh, Dong-Geun;Yoo, Yeong-Jeong
    • Journal of Korea Technical Association of The Pulp and Paper Industry
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    • v.41 no.1
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    • pp.37-43
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    • 2009
  • In this study, the physical properties of base paper for the manufacture of mulching mat for afforestation seedling were investigated. The base paper for mulching mat was prepared by stock conditions of PAE and AKD addition into the screened slurry of KOCC for the strengthening effects of wet tensile and burst strength. The optimum additions of PAE and AKD were considered at 2% and 0.5%, respectively. The accelerated aging by ISO 5630-1 and wet heat aging method under hot water for 2 kinds of commercial mulching and wet strength paper were compared with the base paper prepared for mulching mat manufacture. The accelerated aging test for the base paper prepared for mulching mat manufacture resulted in the same tendency of physical properties as two kinds of commercial products. However, the results of wet heat aging test under hot water indicated that the physical strength for base paper prepared was much higher than others. In addition, the opacity behavior for base paper prepared was enough effects to obstruct weeds growth by isolating transmission of sunlight.

Effects of Pinus rigida Extract of Isozyme Patterns of Glycine max Callus (대두 캘러스의 동위효소 패턴에 미치는 리기다소나무 추출액의 영향)

  • 김용옥;장남기;이호준;은무영
    • The Korean Journal of Ecology
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    • v.20 no.4
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    • pp.245-250
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    • 1997
  • The seeds and callus induced from the root of Glycine max were used as test materials. When the seed was treated with the different concentrations of Pinus rigida extract, there was a more striking inhibition of seedling growth than of seed germination. The callus of the control group was in good condition, but when treated with 5% extract there was generalized browning and cell division was decreased in the upper part of the callus. There was no difference in the fresh and dry weights in 2% extract treatment but there was dramatic repression at concentrations higher than 5%. The band activity of peroxidase isozyme in treated callus was elevated, while that of esterase was inhibited.

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Seedling Emergence and Growth Affected by Priming and $GA_3$ Treatments to Three Campanulan Plant Seeds (Priming과 $GA_3$ 처리에 따른 도라지, 더덕 및 만삼의 출아(出芽)와 생장(生長))

  • Kang, Jin-Ho;Kim, Dong-Il;Kang, Shin-Yun;Shim, Young-Do;Han, Kyung-Soo
    • Korean Journal of Medicinal Crop Science
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    • v.5 no.4
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    • pp.307-313
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    • 1997
  • Seed germination test done in laboratory does not coincide with field emergence in general. The experiments were carried out to examine the effect of priming and $GA_3$, treatment to seeds of Platycodon grandiflorum; Codonopsis lanceolata and C. pilosula on lapsed time to first seedling emergence, seedling emergence, morphological characters and growth and the cause of poor emergence of C. pilosula. No-treatment as Control (water), priming or $GA_3$ treatment was done with only distilled water for 2 days, $CA(NO_3)_2$ 150 mM for 2 days or $GA_3$ 0.1 mM for 3 days, respectively. Seedling emergence rate was counted every 2 days but morphological characters and dry weight of shoot and root were measured on 38 days after sowing. Their internal seed structures were examined with Scanning Electron Microscope. C. pilosula had poorer seedling emergence rate than P grandiflorum and C. lanceolata showing nearly same rate: Compared to the other treatment (s) P. grandiflorum displayed higher rate in priming and $GA_3$, treatments but C. lanceolata or C. pilosula did the greatest rate in only $GA_3$ or priming treatment, respectively. $GA_3$ treatment to seeds of P. grandiflorum and C. lanceolata shortened the lapsed time to seedling emergence in comparison with Control, 2-days water imbibition before sowing. In all the species plant height and number of leaves per seedling became shorter and less in priming treatment than the other treatments except plant height of C. Pilosula while their hypocotyl length was nearly same in all treatments. Although priming treatment had nearly similar effect to morphological characters, $GA_3$ treatment forced greater shoot, root and aftermath total dry weight per seedling. Poor seedling emergence of C. pilosula was caused by its seed defect like cleavage or lack of embryo, poor development of embryo and endosperm or their separation.

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Growth of Seedling from Immature Kernel Harvested at Different Days after Flowering in Rye, Triticale, Wheat and Oat Cultivars (맥류미성숙 종자와 초기생육과의 관계)

  • 황종진;하용웅;연규복
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.32 no.2
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    • pp.188-195
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    • 1987
  • This study was conducted to investigate whether seedling growth be affected by immature kernels from different stages after flowering in rye, triticale, wheat and oats cultivars, for two years in Suwon. Kernels reached a near-maximum test weight at 35 days after flowering in Paldanghomil and Dooroohomil (Rye), and 30 days in Sinkihomil (Triticale), Geurumil (Wheat), and Megwiri (Oats). Test weight of immature kernels from different days after flowering were not significantly correlated with germination percentage, but highly positively correlated with their seedling height, fresh and dry weight. However, seedling height is not different among seedlings from kernels harvested at 20, 25 and 30 days (maturing time) after flowering of Sinkihomil and Megwiri, and between 25 and 30 days (maturing time) of Geurumil. Also between seedlings from 25 day and 30 day-kernel after flowering are little difference of fresh and dry weight of Sinkihomil and Megwiri. In Suwon, located at the middle part of Korea, it was suggested that Paldanghomi1 and Dooroohomil could be harvested at 35 days (June 19, and June 24, respectively) after flowering, and Sinkihomil, Geurumil, and Megwiri, at 25days (June 24, June 14 and July 4, respectively) after flowering for seed of for2:ge production.

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A Study on the Optimum Field Preparation Procedures for the Proper Working Performances of Rice Transplanters (논 써레질한 후의 경과일수 및 담수심이 수도이앙기의 작업성능에 미치는 영향)

  • 홍종호;차균도
    • Magazine of the Korean Society of Agricultural Engineers
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    • v.21 no.3
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    • pp.83-91
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    • 1979
  • This study was conducted in order to find out the performance of rice transplanters in accordance with the change of the trans-planting days after pudding and the water depth flooding the paddy field at the time of transplanting : and thus to select the optimum paddy field preparation procedures for an efficient utilization of rice transplanters. The performance factors of the two different types of rice transplanters were measured during the first 6 consecutive days after puddling and with 3 different levels of water depth flooding the paddy fields. The results of this study were analysed and summarized as follows : 1. Wheel sinkage decreased very rapidly from 0 to 2 days after puddling and slowly from 3 to 5 days after puddling. 2. The depth of the test cone penetration decreased rapidly during the first few days after puddling. It was 17.8cm just after puddling, and decreased to 13.4cm one day after puddling. After 2 days, the rate of decrease was dampened, and after 5 days it kept constant value of 9.2cm. 3. Two days after puddling, the hill interval was 15.8cm (98.75% of the preset value) for broadcasted seedling rice transplanter with 3cm flooding depth : This value was the closest to the pre-adjusted value of 16cm. The general performance of broadcasted-seedling type rice transplanter was better than that of strip-seedling type rice transplanter. 4. Usually the working performance of a rice transplanter is evaluated with uniformity and adjustability of the hill intervals. The hill interval was the most uniform and closest to the pre-set value of 16cm when planted two days after puddling with 3cm of water depth. When it was inavoidable to plant 4 days after puddling with stripseedling type rice transplanter, it is advisable to let the water flooded somewhat deeper. 5. The percentage of missing hills including floating and burried seedlings was the highest just after puddling and ie decreased substancially until 3 days after puddling and then it increased again. Hence, the optimal time transplanting is to be between 2 and 3 days after puddling. 6. Better postures of planted seedlings were found when planter 2 days after puddling than 3 days after puddling. Six cm of flooding water depth always gave the best results with respect to the postures of planted seedlings. Broadcasted-seedling rice transplanter, in general, showed better posture of planted seedlings than did strip-seedling type rice transplanter. 7. Judging from the above results, the optimal conditions will be 3cm of flooding depth and transplanting between 2 and 3 days after puddling.

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In Fluence Chemicals From Artemisis argyi on the Growth of Selected Species of Plants and Microorganisms (황해쑥에 함유된 화학물질이 다른 식물과 미생물의 생장에 미치는 영향)

  • 길봉석;윤경원;이순엽;한동민
    • The Korean Journal of Ecology
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    • v.17 no.1
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    • pp.23-35
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    • 1994
  • To investigate phytotoxic substances in Artemisia argyi, the donor plant, and their biological activities, seed germination and seedling growth of receptor plants such as Arundinella hirta, Echinochloa crus-galli, Rumex crispus and Lactuca sativa were examined at different concentrations of aqueous extracts of the donor plant. Germination of four receptor species was inhibited by the extracts, while seedling growth was decreased to a lesser degree than in the germintion test. Germination, seedling growth and dry weight growth of Achyranthes japonica grown in pot were proportionally inhibited by the extracts. Volatile substances emitted from A, argi plant caused slight inhibition in the germination and seedling growth of the receptor species. Essential oil of the plant extracted by Karlsruker's apparatus inhibited growth of microorganisms and callus growth of Pinellia ternata and Oryza sativa. The GC /MS method was employed for analysis and identification of allelochemicals from A. argyi leaves. Sixty-one chemical substances such as a-pinene, camphene, 1. 8-cineol, etc. were identified from essential oil of A. argyi. The results of this experiment on seed germination, seedling growth, microorganism culture and tissue culture indicated that naturally occurring chemical substances from A. argyi would be responsible for the growth inhibition of plants studied.

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The Early Growth Performances of Pinus densiflora and Larix kaempferi Seedlings Under Open-field Experimental Warming and Precipitation Manipulation (실외 실험적 온난화 및 강수 처리에 따른 소나무와 낙엽송 유묘의 초기 생장 특성)

  • Kwon, Boram;Cho, Min Seok;Yang, A-Ram;Chang, Hanna;An, Jiae;Son, Yowhan
    • Journal of Korean Society of Forest Science
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    • v.109 no.1
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    • pp.31-40
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    • 2020
  • This study aimed to investigate the effects of climate change on the survival and growth performance of Pinus densiflora and Larix kaempferi seedlings using open-field experimental warming and precipitation manipulation. We measured the survival rate, root-collar diameter, and height, and then calculated the seedling quality index (SQI) of 2-year-old seedlings under 6 treatments [2 temperatures (TC: Control; TW: Warming) × 3 precipitation manipulations (PC: Control; PD: Decreased; PI: Increased)] and performed a two-way ANOVA to test for differences.The air temperature of the warming plots was 3℃ higher than that of the control plots, while the precipitation manipulation plots received ±40% of the precipitation received by the control plots. Temperature and precipitation treatments did not significantly affect the survival rate of P. densiflora; however, the SQI of P. densiflora decreased with increasing precipitation. In contrast, the mortality rate of L. kaempferi increased with increasing temperature and decreasing precipitation. Furthermore, in L. kaempferi, TC × PI treatment resulted in the lowest SQI with a significant interaction effect observed between the two factors. In summary, low seedling production and quality should be expected in P. densiflora as precipitation increases and in L. kaempferi as temperature increases or precipitation decreases. These results indicate species-specific sensitivities to climate change of two plant species at the nursery stage. With the occurrence of global warming, the frequencies of drought and heavy rainfall events are increased, and this could affect the survival and seedling quality of tree species. Therefore, it is necessary to improve nursery techniques by establishing new adaptation strategies based on species-specific growth performance responses.