• 한국환경복원기술학회지
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• 제13권6호
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• pp.87-96
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• 2010

This study was conducted to select environmentally-friendly and low-cost mulching material that could replace soil molding which can be used to restore vegetation in an abandoned coal mine area. To this end, we established 20 experimental plots (4m ${\times}$ 10m in size) on the steep, south west-facing slope of the abandoned coal mine area in Hwangji-Dong, Taebaek City, Gangwon Province in April 2006. We planted two-year-old 1,600 seedlings (at intervals of 0.6m ${\times}$ 0.8m) of drought-resistant tree species including Betula schmidtii, Betula platyphylla var. japonica, Amorpha fruticosa, and Quercus mongolica in the plots. After planting seedlings, mulching was applied by using five different kinds of material such as HWAP (Teracotem), peat moss, straw mats, wood chips, and control (no-mulching) and the effects of different mulching material on the survival rate and growth performance were compared. Three years after planting, the survival rate was the highest in wood chip mulching, followed by straw-mat, peat moss, HWAP, and control. The survival rate was the highest in Quercus mongolica, followed by Betula schmidtii, Betula platyphylla var. japonica, and Amorpha fruticosa. Meanwhile the height growth was the best in Betula platyphylla var. japonica, followed by Betula schmidtii, Quercus mongolica, and Amorpha fruticosa. The height growth of seedlings was the best in HWAP mulching, followed by peat moss, woody chips, straw mat, and control. From an economic point of view, wood chips are considered to be the best mulching material. The results showed that mulching without soil molding and/or mixing applications would be effective for restoring vegetation in an abandoned coal mine areas.

• 한국양식학회지
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• 제11권2호
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• pp.183-191
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• 1998

조개류의 인공종묘생산시 생멱이생물의 대체먹이원을 개발하기 위해 냉동 EH는 건조한 먹이생물의 먹이효율을 조사하였다. T. suecica를 대상으로 실험구를 생 T. suecica, 건조 T. suecica, 냉동 T. suecica, 생 T. suecica(50%)+냉동 T. suecica(50%), 생 T. suecica(50%)건조 T. suecica(50%)를 구분하여 참굴과 개량조개 유생 그리고 바지락 치패에게 먹이로 공급하며 먹이효율을 비교하였다. 참굴 유생의 경우는 생 T. suecica를 공급한 실험구가 각고 $231.9^{\mu}$m로 성장이 가장 좋았고, 생존율은 72.6%로 가장 높게 나타났으며 다른 실험구와 유의적인 차이를 보였으며 건조 T. suecica를 단독으로 공급한 실험구에 각고 $168.7^{\mu}$m, 생존율 39.3%로 가장 저조한 것으로 나타났다. 개량조개 유생에서는 생 T. suecica를 공급한 실험구가 각장 $213.0^{\mu}$m, 생존율 42.9%로 가장 좋았으나 다른 실험구와 유의적인 차이가 없었다. 바지락 소형 치패의 경우 성장은 생 T. suecica, 생 T. suecica(50%)+냉동T. suecica50%)를 공급한 실험구와 다른 실험구와 유의적인 차이를 보였다. 그리고 대형 치패 경우 성장은 생 T. suecica, 생 T. suecica(50%)+건조 T. suecica(50%) 실험구와 유의적인 차이를 나타냈다. 그러나 바지락 치패의 생존율에서는 유의적인 차이를 보이지 않았다. 이와같이 냉동, 건조된 T. suecica의 먹이효율은 유생의 종류와 성장단계에 따라 다른 것으로 나타났으며 생 T. suecica를 부분적으로 대체할 수 있을 것으로 판단된다.

• 생물환경조절학회지
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• 제29권3호
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• pp.219-230
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• 2020

본 연구는 시설 딸기(Fragaria × ananassa Duch. cv. Maehyang)의 삽목묘 생산을 위해 적절한 배지 및 가습 기간을 구명하기 위해 수행되었다. 2019년 2월에 코이어(CO), 암면(RW), 페놀폼(PF), 유기성형배지(OFM)에 삽수를 삽목하여 0, 3, 6, 9, 12일간 포깅 처리를 하였으며, 2019년 6월에 현장실증 실험으로 코이어(CO), 암면(RW), 페놀폼(PF), 유기성형배지(OFM)에 삽수를 삽목하여 0, 6, 9, 12, 15일간 미스팅 처리를 하였다. 포깅 처리에서 삽목묘의 발근율과 생존율은 포깅 기간이 길어질수록 증가하는 경향을 보였으며, CO 배지에서 9일 이상 포깅 처리하였을 때 발근율과 생존율이 유의적으로 높았다. 미스팅 처리에서 발근율은 CO와 RW 배지에서 9일 이상 미스팅 처리하였을 때, 생존율은 12일 이상 미스팅 처리하였을 때 유의적으로 높았다. 육묘기에 배지 및 가습 처리에 따른 지상부와 지하부의 생육은 유의적인 차이가 없었다. 따라서 발근율과 생존율을 고려하였을 때, CO 배지에서 9일간 가습 처리하는 것이 안정적인 삽목묘의 생산에 유리할 것으로 판단된다.

• 한국산림과학회지
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• 제95권6호
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• pp.636-642
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• 2006

야외에서 새로운 송이균환을 만들기 위하여 2000년부터 2005년까지 소나무를 이용한 송이 감염묘 생산과 이식 및 이식 후 송이 균의 활착을 높일 수 있는 방법을 연구하였다. 송이 간염묘 생산을 위해서는 11월에 균환 선단에 심을 경우 활착률 97%를 나타낸 반면 4월에 심을 경우에는 활착률이 80%를 나타냈다. 송이균의 감염은 식재 후 2년 이상 경과하여야 완전히 이루어지는 것으로 나타났으며, 천연치수를 이용한 경우는 17.6%의 송이균 생존률을 나타낸 반면, 양묘를 이용한 경우는 송이균의 생존율이 극히 낮았다. 한편, 생산된 감염묘의 이식 시 송이균의 생존율은 4월에 이식할 경우 22%, 10월과 11월에 이식할 경우에는 5%를 나타냈다. 이식한 송이 감염묘에서 송이균의 생존은 이식전 50% 내외의 송이균 감염율을 가진 감염묘가 가장 높은 생존율을 니타냈다. 이식한 송이 감염묘에서 송이균의 생존 여부 확인을 위하여 송이 감염묘를 완전히 파내어 확인하는 방법은 살아있던 송이균도 이후에 사멸하게 되는 원인이 되었다. 이에 따라 이식한 감염묘의 송이균 확인은 색대를 이용하는 방법을 도입하였으며, 이식 후 2년 이상 경과한 후에 검정하는 것이 송이균의 생존에 유리할 것으로 판단되었다.

• 한국수산과학회지
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• 제32권5호
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• pp.654-658
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• 1999

패류 인공종묘생산시 양질의 먹이를 원활하게 공급하기 위하여 장기간 보관이 가능한 건조먹이를 생산하여, 굴 유생을 대상으로 이용 가능성을 조사하였다. 이를 위하여 건조먹이의 생화학성분과 지방산 조성을 분석한 결과, 생화학성분 조성은 생먹이 및 농축먹이와 차이가 없었다. 지방산 조성은 건조먹이가 생먹이보다 PUFA DHA 및 EPA의 비율이 높았으며 (p<0.05) 특히 EPA는 생먹이보다 7.35배 높았다. 농축먹이에 건조먹이를 비율별로 혼합하여 굴 유생을 15일간 사육한 후 유생의 생존율과 지질 함량을 분석한 결과, 건조먹이를 $30\%$까지 혼합하여 사용하여도 생먹이만을 공급한 것보다 굴 유생의 생존율이 향상되었으며, 그중 건조먹이를 $10\%$ 혼합하여 사육하면 생먹이만으로 사육한 것보다 생존율이 2.1배 향상되었다(p<0.05) 15일째 굴 유생의 지질 함량은 건조먹이를 $10\%$ 혼합하여 사육한 경우 생먹이만을 공급한 것보다 약 1.6배 높았다. 따라서 패류의 종묘를 생산하지 않는 시기에 먹이생물을 배양하여 건조먹이 형태로 보관하여 두었다가, 농축먹이와 혼합하여 사용한다면, 보다 간편하게 먹이를 공급할 수도 있으며 아울러 유생의 생존율도 향상시킬 수 있을 것으로 기대된다.

• 한국토양비료학회지
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• 제46권6호
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• pp.606-609
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• 2013

To investigate the effect of partial tillage seeding of hairy vetch on green manure biomass and rice yield, on-farm experiment was conducted at eco-friendly hairy vetch cultivation area located in Yesan-gun, Chungcheongnam-do. Seeding methods of hairy vetch consisted of partial tillage seeding (PTS) and broadcasting before rice harvesting (BBRH). Hairy vetch was incorporated into soil on May 18 and rice seedling was transplanted on May 26. The growths of hairy vetch before overwintering were investigated on November 11. Plant height of BBRH plot was longer than that of PTS plot, but somewhat larger number of seedling stand was found in PTS. Biomass and N production of hairy vetch were investigated on May 18. Results of the investigation showed no difference between two seeding methods. Although the seeding time was 21 days late in PTS, the biomass of hairy vetch and rice yield were equivalent to those of BBRH seeding, so we conclude that PTS could improve overwintering survival of hairy vetch in rice cropping system.

• 농업과학연구
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• 제46권4호
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• pp.781-790
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• 2019

Changes in the physical and chemical properties of soil materials during the nursing process have a great influence on the quality of containerized seedlings and on growth and survival after planting. In this study, the effect of biomaterials and their mixed ratios on the growth of Zelkova serrata seedlings in a containerized seedling production system was investigated. Mushroom sawdust, pine bark, and carbonized rice husk were used as biomaterials. The mixed ratios were 10% and 20% volume ratio of the growing medium volume, including the untreated controls. There was no significant difference in the height growth of the Zelkova serrata seedlings according to the biomaterials. The root collar diameter was the highest with the 20% carbonized rice husk and the lowest with the mushroom sawdust. The difference between the highest quality index and the lowest quality index was 30% in the order of the carbonized rice husk, pine bark, control, and mushroom sawdust, but there was no statistical significance. In this study, if the growing medium mixed with biomaterials does not reduce the seedling growth compared with the control, it is considered that the biomaterial can replace a part of the growing media. Therefore, the results show that some of the growing media can be replaced with carbonized rice husk or pine bark when producing Zelkova serrata seedlings.

• Journal of Ecology and Environment
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• 제41권8호
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• pp.202-212
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• 2017

Background: To reveal establishment strategy of Sparganium erectum, we tried to find realized niche of adults through field survey and effects of water level on the establishment process through mesocosm experiments. Results: In the field survey, the height and coverage of community living in deeper water were greater than those of community living in shallow water. There was no statistically significant difference (p > 0.05) in the means of water and soil properties between the two communities. In mesocosm experiments, we found no correlation between water levels and germination rates, but S. erectum seedlings have characteristics of post germination seedling buoyancy when S. erectum seeds germinated in inundation conditions. Shoot height, total leaf length, and survival rates of sinking seedlings in shallow water levels at -5, 0, and 5 cm were higher than those in deeper water levels at 10 and 20 cm. Floating seedlings established in water levels of 3 and 6 cm only. The seedlings could live up to 6 weeks in floating state but died if they were unable to establish. Conclusions: The water level around adult S. erectum communities in the field were different from the water level at which S. erectum seedlings can survive in the mesocosm experiments. The findings provided not only understanding of S. erectum habitat characteristics but also evidence to connect historical links between the early seedlings stage and adult habitat conditions. We suggested the logical establishment strategy of S. erectum based on the data.

• Journal of Plant Biotechnology
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• 제47권3호
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• pp.235-241
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• 2020

This is the first study to establish a complete protocol for micropropagation of Rehmannia glutinosa from root segments. The study involved investigating the effect of plant growth regulators on in vitro shoot regeneration and rooting and identifying substrates supporting survival and growth performance of ex vitro seedlings. A Murashige and Skoog (MS) medium containing 30 g/L sucrose for shoot induction and 0.2 mg/L indole-3-acetic acid (IAA), 1 mg/L 6-benzylaminopurine (BAP), and 1 g/L polyvinylpyrrolidone (PVP) for shoot multiplication resulted in the highest number of shoots per explant and shoot height. Applying a medium containing 0.5 mg/L IAA and 1 g/L PVP yielded optimal rooting of the shoots grown in vitro. Compost enriched with microbial inoculants and perlite enhanced seedling growth better than that with organic biofertilizer-free substrates (soil and sand). We recommend the continuous production of micropropagated R. glutinosa seedlings from root segments under the aforementioned conditions as a possible propagation technique for crops of this species.

• Journal of Biosystems Engineering
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• 제26권4호
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• pp.379-384
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• 2001

To enhance the smooth graft-taking of grafted seedlings, the excessive evapotranspiration from grafted seedlings just after grafting should be avoided. A measurement system of the evapotranspiration rate of grafted seedling under artificial lighting was set up to investigate the effect of physical environment on the evapotranspiration and graft-taking characteristics of grafted seedlings quantitatively. The evapotranspiration rate of grafted seedlings affected by relative humidity and light intensity were analyzed using the measurement system. The hypocotyl of watermelons (Citrullus vulgaris cv. Sweetdew, Hungnong Seed Co.) was slantly cut and then inserted into a hole on the stem of rootstock (Lagenaria siceraria cv. FR-King, Hungnong Seed Co.). Grafted seedlings were healed and joined for 5 days under cool-white fluorescent lamps (FL20SEX-d/18, Keumho Electric Co.) with photoperiod of 12h$.$d$\^$-1/ except dark period for one day after grafting in a closed graft-taking enhancement system developed by Kim(2000). The evapotranspiration rate and graft-taking of grafted seedling at air temperature of 23$\^{C}$ and air current speed of 0.1m$.$s$\^$-1/ was highly affected by relative humidity. But light intensity showed higher effect on the stem length of scion than relative humidity. In conclusion, it was suggested that relative humidity should be controlled at higher than 90% with photosynthetic photon flux of 50$\mu$mol$.$m$^2$$.$s$\^$-1/ to increase the survival of grafted seedlings and to produce healthy seedlings.