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http://dx.doi.org/10.5010/JPB.2020.47.3.235

Micropropagation from root segments to improve seedling quality in Chinese foxglove crops  

Pham, Thanh Loan (Institute of Applied Research and Development, Hung Vuong University)
Nguyen, Van Huy (Institute of Applied Research and Development, Hung Vuong University)
Hoang, Thi Le Thu (Institute of Applied Research and Development, Hung Vuong University)
Ha, Thi Tam Tien (Institute of Applied Research and Development, Hung Vuong University)
Tran, Trung Kien (Institute of Applied Research and Development, Hung Vuong University)
Vu, Xuan Duong (Institute of Applied Research and Development, Hung Vuong University)
Cao, Phi Bang (Faculty of Natural Sciences, Hung Vuong University)
Nguyen, Quang Trung (Faculty of Agriculture, Hung Vuong University)
Publication Information
Journal of Plant Biotechnology / v.47, no.3, 2020 , pp. 235-241 More about this Journal
Abstract
This is the first study to establish a complete protocol for micropropagation of Rehmannia glutinosa from root segments. The study involved investigating the effect of plant growth regulators on in vitro shoot regeneration and rooting and identifying substrates supporting survival and growth performance of ex vitro seedlings. A Murashige and Skoog (MS) medium containing 30 g/L sucrose for shoot induction and 0.2 mg/L indole-3-acetic acid (IAA), 1 mg/L 6-benzylaminopurine (BAP), and 1 g/L polyvinylpyrrolidone (PVP) for shoot multiplication resulted in the highest number of shoots per explant and shoot height. Applying a medium containing 0.5 mg/L IAA and 1 g/L PVP yielded optimal rooting of the shoots grown in vitro. Compost enriched with microbial inoculants and perlite enhanced seedling growth better than that with organic biofertilizer-free substrates (soil and sand). We recommend the continuous production of micropropagated R. glutinosa seedlings from root segments under the aforementioned conditions as a possible propagation technique for crops of this species.
Keywords
IAA; Multiple shoots; NAA; Rehmannia; PVP; Root induction; Tissue culture;
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1 Abohatem M (2011) Arbuscular mycorrhizal fungilimit incidence of Fusarium oxysporum f.sp. albedinis on date palm seedlings by increasing nutrient contents, total phenols and peroxidase activities. Open Hortic J 4:10-16   DOI
2 Akhzari D, Attaeian B, Arami A, Mahmoodi F, Aslani F (2015) Effects of vermicompost and arbuscular mycorrhizal fungi on soil properties and growth of Medicago polymorpha L. Compost Sci Util 23:142-153   DOI
3 Barje F, Meddich A, El Hajjouji H, El Asli A, Ait Baddi G, El Faiz A, Hafidi M (2016) Growth of date palm (Phoenix dactylifera L.) in composts of olive oil mill waste with organic household refuse. Compost Sci Util 24:273-280   DOI
4 Cuenca S, Amo-Marco JB (2000) In vitro propagation of two spanish endemic species of salvia throught bud proliferation. Vitr Cell Dev Biol - Plant 36:225-229   DOI
5 Du J-F, Yin W-J, Zhang Z, Hou J, Huang J, Li J (2009) Autotoxicity and phenolic acids content in soils with different planting interval years of Rehmannia glutinosa. Chinese J Ecol 28: 445-450
6 Hunkova J, Gajdosova A (2019) In vitro rooting and acclimatization of Amelanchier alnifolia (Nutt.) Nutt. ex M. Roem: Testing of auxin, spermidine, and gibberellin for overcoming dormancy. J Berry Res 9:549-561   DOI
7 Faisal M, Ahmad N, Anis M, Alatar AA, Qahtan AA (2018) Auxin-cytokinin synergism in vitro for producing genetically stable plants of Ruta graveolens using shoot tip meristems. Saudi J Biol Sci 25:273-277   DOI
8 Getnet B (2017) In vitro shoot multiplication of two sugarcane (Saccharum officinarum L.) genotypes using shoot apical meristem. Adv Life Sci Technol 53:13-19
9 Gupta S, Kachhwaha S, Kothari SL, Jain R (2020) Synergistic effect of cytokinins and auxins enables mass clonal multiplication of drumstick tree (Moringa oleifera Lam.): a wonder. Vitr Cell Dev Biol - Plant 1-12.
10 Jan B, Ali A, Wahid F, Shah SNM, Khan A, Khan F (2014) Effect of Arbuscular mycorrhiza fungal inoculation with compost on y ield and phosphorous uptake of Berseem in alkaline calcareous soil. Am J Plant Sci 05:1359-1369   DOI
11 Jimenez-Marina L, Fonseca-Arias M, Garcia-Alcantara A, Infante-Fonseca S, Vazquez-Rodriguez J (2019) Effect of different concentrations of indole acetic acid (IAA) in the in vitro rooting of Dahlia sp. Cultiv Trop 40:e11
12 Kang K, Kim C, Sohn D, Yoon H, Jung H (2011) Inhibitory effect of Rehmannia glutinosa pharmacopuncture solution on $\beta$-hexosaminidase release and cytokine production via immunoglobulin receptor signaling in RBL-2H3 Cells. JAMS 4:269
13 Knudson L (1946) A new nutrient solution for the germination of orchid seed. Amer Orchid Soc Bull 15:214-217
14 Murashige T, Skoog F (1962) An ellagic compound and iridoids from Cornus capitata root cultures. Physiol Plant 15:473-497   DOI
15 Liu C, Cheng L, Ko C, Wong C, Cheng W (2012) Bioassay-guided isolation of anti-inflammatory components from the root of Rehmannia glutinosa and its underlying mechanism via inhibition of iNOS pathway. J Ethnopharmacol 143:867-875   DOI
16 Mao WY, Li XG, Zhu BM (1983) Studies on the meristern culture of Rehmannia glutinosa. Chin Bull Bot 1:44-46
17 Mrabet S El, Ouahmane L, Mousadik A El, Msanda F, Abbas Y (2014) The effectiveness of arbuscular mycorrhizal inoculation and bio-compost addition for enhancing reforestation with Argania spinosa in Morocco. Open J For 04:14-23
18 Nguyen TTT (2016) Establishment Rehmannia glutinosa crops for medicinal materials followed GACP-WHO guidelines and processing some medicinal products. Final report. Bac Giang Pharmacy Joint-Stock Company
19 Piatczak E, Grzegorczyk-Karolak I, Wysokinska H (2014a) Micropropagation of Rehmannia glutinosa Libosch.: Production of phenolics and flavonoids and evaluation of antioxidant activity. Acta Physiol Plant 36:1693-1702   DOI
20 Piatczak E, Kuzma L, Sitarek P, Wysokinska H (2014b) Shoot organogenesis, molecular analysis and secondary metabolite production of micropropagated Rehmannia glutinosa Libosch. Plant Cell Tissue Organ Cult 120:539-549   DOI
21 Podwyszynska M, Sowik I, Machlanska A, Kruczynska D, Dyki B (2017) In vitro tetraploid induction of Malus domestica Borkh. using leaf or shoot explants. Sci Hortic (Amsterdam) 226:379-388   DOI
22 Shoyama Y, Nagano M, Nishioka I (1983) Clonal multiplication of Rehmannia glutinosa. Planta Med 48:124-125   DOI
23 Praveena C, Veeresham C (2014) Multiple shoot regeneration and effect of sugars on growth and nitidine accumulation in shoot cultures of Toddalia asiatica. Pharmacogn Mag 10:S480-S486
24 Prayudyaningsih R, Sari R (2016) The application of arbuscular mycorrhizal fungi (AMF) and compost to improve the growth of Teak seedlings (Tectona grandis Linn. f.) on limestone post-mining soil. J Penelit Kehutan Wallacea 5:37-46   DOI
25 Sang UP, Yong KK, Sook YL (2009) Improved invitro plant regeneration and micro propagation of Rehmannia glutinosa L. J Med Plants Res 3:031-034
26 Sarwar G, Schmeisky H, Hussain N, Muhammad S, Ibrahim M, Safdar E (2008) Improvement of soil physical and chemical properties with compost application in rice-wheat cropping system. Pakistan J Bot 40:275-282
27 Schultz C (2001) Effect of (vesicular-) arbuscular mycorrhizs on survival and post vitro development of micropropagated oil palms (Elaeis guineensis Jacq.). Doctoral dissertation, University of Gottingen, Germany
28 Srikanth N, Mangal AK, Mhase AG, Gurav AM, Kolhe R, Goli PP, Nirawane RR (2019) Multiplication of Bharangi - Clerodendrum serratum (L.) Moon: An ayurvedic important plant. J Drug Res Ayurvedic Sci 4:126-131   DOI
29 Thi TMN, Thwe AA, Tuan PA, Chae SC, Park SU (2012) Shoot organogenesis and plant regeneration of Rehmannia elata L. Life Sci J 9:882-885
30 Vacin EF, Went FW (1949) Some pH changes in nutrient solutions. Bot Gaz 110:605-613   DOI
31 Zhang R, Li M, Jia Z (2008) Rehmannia glutinosa: Review of botany, chemistry and pharmacology. J Ethnopharmacol 117:199-214   DOI
32 Wu L, Chen J, Khan MU, Wang J, Wu H, Xiao Z, Zhang Z, Lin W (2018) Rhizosphere fungal community dynamics associated with Rehmannia glutinosa replant disease in a consecutive monoculture regime. Phytopathology 108:1493-1500   DOI
33 Xu XH, Davey MR (1983) Shoot regeneration from mesophyll protoplasts and leaf explants of Rehmannia glutinosa. Plant Cell Rep 2:55-57   DOI
34 Yu H, Seo S, Kim Y, Lee H, Park R, So H, You Y (2006) Protective effect of Rehmannia glutinosa on the cisplatin-induced damage of HEI-OC1 auditory cells through scavenging free radicals. 107:383-388   DOI
35 Zamir R, Rab A, Sajid M, Khalil SA, Shah ST (2017) Effect of different auxins on rooting of semi hard and soft wood cuttings of guava (Psidium guajava L.) CV . Safeda. The Nucleous 54:46-51
36 Zhang B, Weston PA, Gu L, Zhang B, Li M, Wang F, Tu W, Wang J, Weston LA, Zhang Z (2019) Identification of phytotoxic metabolites released from Rehmannia glutinosa suggest their importance in the formation of its replant problem. Plant Soil 441:439-454   DOI
37 Zhang Z, Lin W, Yang Y, Chen H, Chen X (2011) Effects of consecutively monocultured Rehmannia glutinosa L. on diversity of fungal community in rhizospheric soil. Agric Sci China 10:1374-1384   DOI