• Journal of Plant Biotechnology
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• 제33권4호
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• pp.263-270
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• 2006

Pongamia pinnata Pierre is a tree legume, having potential in production of raw material for biodiesel. A protocol for in wk propagation of this plant was standardized using seedling explants. Growth regulators (GR) including gibberellic acid $(GA_3),\;N^6-benzylaminopurine(BA)$, thidiazuron (TDZ), and Adenine sulphate (Ads) were tested for optimum germination of seeds. Removal of seed coat prior to germination, controlled fungal growth partially but enhanced bacterial growth. Antibiotic cefotaxime was ineffective in controlling bacterial contamination. Seedling derived nodal explants and cotyledon nodes with attached cotyledons were excised and cultured for induction of shoots. Optimum sprouting and multiplication of shoot buds were obtained in MS medium supplemented with $8.88{\mu}M$ BA. These buds differentiated and rooted on medium devoid of GR. Optimum growth of Pongamia seedling was obtained in cotton plugged culture vessels. Reculturing of the cotyledon node explants produced more shoots from the same site. This process of removing shoots and reculturing of cotyledon node was followed for eight passages yielding 4 to 8 shoots in each cycle. The shoots (75%) rooted on half strength MS basal medium supplemented with 0.22% charcoal. All plants survived on transfer to soil. This is the first report on in vitro regeneration of Pongamia pinnata. This report demonstrates the possibility of coupling more than one parameter in single experiment to hasten the process of standardization. The process of cycling the nodal explant repeatedly for production of large number of shoots from single meristem may find application in genetic transformation experiments wherein meristems are used for transformation.

• ALGAE
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• 제37권2호
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• pp.105-121
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• 2022

Modern seaweed farming relies heavily on seedlings from natural beds or vegetative cuttings from previous harvests. However, this farming method has some disadvantages, such as physiological variation in the seed stock and decreased genetic variability, which reduces the growth rate, carrageenan yield, and gel strength of the seaweeds. A new method of seedling production that is sustainable, scalable, and produces a large number of high-quality plantlets is needed to support the seaweed farming industry. Recent use of tissue culture and micropropagation techniques in eucheumatoid seaweed production has yielded promising results in increasing seed supply and growing uniform seedlings in large numbers in a shorter time. Several seaweed species have been successfully cultured and regenerated into new plantlets in laboratories using direct regeneration, callus culture, and protoplast culture. The use of biostimulants and plant growth regulators in culture media increases the seedling quality even further. Seedlings produced by micropropagation grew faster and had better biochemical properties than conventionally cultivated seedlings. Before being transferred to a land-based grow-out system or ocean nets for farming, tissue-cultured seedlings were recommended to undergo an acclimatization process to increase their survival rate. Regular monitoring is needed to prevent disease and pest infestations and grazing by herbivorous fish and turtles during the farming process. The current review discusses recent techniques for producing eucheumatoid and other valuable seaweed farming materials, emphasizing the efficiency of micropropagation and the transition from laboratory culture to cultivation in land-based or open-sea grow-out systems to elucidate optimal conditions for sustainable seaweed production.

• 생물환경조절학회지
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• 제11권1호
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• pp.12-17
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• 2002

수출용 토마토 하계육묘시 육묘 용기 와 육묘 일수를 달리 함으로써 나타나는 묘소질의 차이를 구명하고, 묘소질이 다른 묘를 정식하여 재배할 경우 생육과 수량에 미치는 영향을 구명코자 하였다. 육묘시의 묘 생육은 폿트 묘가 플러그 묘보다 좋았으며, 폿트 묘는 육묘 일수가 길수록 좋았으나 플러그 묘는 육묘 일수에 따른 차이가 없었다. 정식 60일 후의 생육도 정식 직전과 같은 경향이었다. 1화방 수확 개시기는 폿트 묘가 플러그 묘보다 빨랐으며, 육묘 일수가 길수록 빨랐다. 4개월간 수량은 폿트 묘가 플러그 묘 보다 유의하게 많았는데 수확 2개월까지 초기수량이 현저히 많았다. 폿트 묘는 수확 2개월까지는 45일 묘와 35일 묘는 큰 차이가 없었고 25일 묘가 가장 적었으나 수확 3개월째부터는 육묘일수에 따른 차이가 없었다. 플러그 묘에서는 수확 3개월가지는 35일 묘가 가장 많았고 25일 묘, 45일 묘 순이었으나 수확 4개월째에는 육묘 일수에 따른 차이가 없었다. 따라서 수출용 모모타로 요쿠 재배시, 수출시기와 수출기간에 따라 육묘 용기 용량 및 육묘 일수를 결정하는 것이 좋을 것으로 생각되었다.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.327-327
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• 2017

The effect of different seedling ages and transplanting times on the growth and yield of Indica ${\times}$ Japonica rice for noodle processing was evaluated to develop a high yielding cultivation technology for increasing the competition against the imported foreign rice. Four seedling ages (10-, 20-, 30- and 33-day old) of two Indica ${\times}$ Japonica rice cultivars (cvs. Saemimyeon and Palbangmi) and three transplanting dates (May 20, May 30 and June 9) were used in the study. Our results showed that the growth and rice yield of the two cultivars were significantly affected by the different seedling ages and transplanting times. Dry matter production at the panicle heading of the two rice cultivars were generally higher in the 30-day old seedling than the other seedling age treatments and then gradually decreased as the transplanting time was delayed from May 20 to June 9. Similar high panicle number per square meter were recorded at the 30-day old seedling between May 20-May 30 transplanting times. In contrast, other yield parameters that includes spikelet number per panicle, 1,000-brown rice weight, and ripened grain ratio (except for the June 9 transplanting time of Palbangmi) were not significantly affected. The milled rice yield of Saemimyeon was higher than that of the Palbangmi regardless of seedling ages and it was also the highest at the 30-day old seedling with four seedling ages. The milled rice yield of Saemimyeon was only slightly decreased as the transplanting time was delayed from the May 20 to June 9 but Palbangmi had a significantly lower milled rice yield at June 9 transplanting due to the low ripened grain ratio. The result indicates that the most suitable seedling age for the cultivars tested was at 30-day old seedling for noodle processing rice and recommended transplanting times were between May 20 and May 30 for the high rice yield in the Yeongnam plain area.

• 한국농림기상학회:학술대회논문집
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• 한국농림기상학회 2005년도 추계 학술발표논문집
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• pp.240-246
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• 2005

• 생물환경조절학회지
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• 제29권2호
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• pp.130-140
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• 2020

본 연구는 대체에너지, 생태복원 및 밀원식물용 등으로 이용되어 묘목 수요가 증가되고 있는 쉬나무의 우량한 용기묘 생산을 위한 적정 차광수준을 구명하고자 실시하였다. 공시용기는 임업시설양묘용 플라스틱 용기(350ml/구)를 사용하였다. 차광수준은 전광과 전광의 35%, 55%, 75%로 처리하였다. 실험결과, 간장과 근원경 생장은 전광에서 현저히 높은 값을 보였으며, 차광수준이 강해질수록 낮은 생장값으로 조사되었다. 묘목의 뿌리형태특성을 측정한 결과 전광에서 뿌리발달이 가장 왕성하게 조사되었다. 건물생산량도 전광에서 가장 높았고 전체적으로 간장 및 근원경 생장 결과와 유사한 경향으로 나타났다. 묘목의 품질을 나타내는 지수인 QI(Quality Index)도 차광실험의 경우 전광에서 0.98로 가장 높게 조사되었다. 묘목의 엽록소 함량은 상대적으로 차광수준이 가장 높은 75% 차광처리에서 가장 높은 엽록소 함량을 보였으며, 광합성속도 및 수분이용효율은 전광에서 각각 8.48μmolCO₂·m^-2s^-1, 1.40μmolCO₂·mmolm^-1H₂O 가장 높은 것으로 조사되었다. 연구결과를 종합하면, 쉬나무 용기묘의 우량한 묘목생산을 위한 적정 차광수준은 전광(0%)으로 판단되며, 쉬나무 용기묘의 대량생산을 위한 기초자료로 활용될 것으로 기대된다.

• 한국환경과학회지
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• 제25권6호
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• pp.889-895
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• 2016

In Danseong-myeon (myeon is a subdivision of), Sancheong-gun, and Sugok-myeon, Jinju City, pot seedling culture was used at the rates of 100% and 62%, respectively. Root restriction seedling culture was used relatively more frequently than pot seedling culture in Yangchon-myeon, Nonsan City, Guryong-myeon, Buyeo-gun, and Wolsan-myeon, Damyang-gun. Open-field seedling culture was used at the rates of 80% and 54% in Samnangjin-eup (eup is a subdivision of a gun), Miryang City and Ssangnim-myeon, Goryeong-gun, respectively. In Danseong, Sancheong, and Sugok, Jinju, granite soil was used as the medium for pot seedling culture at the rates of 90% and 80%, respectively. In Yangchon, Nonsan, Wolsan, Damyang, and Ssangnim, Goryeong, commercial bed soil and coir dust were used for seedling culture at the rates of 73%, 64%, and 60%, respectively. At the main production sites, the use of granite soil for seedling culture, highest rate in 2000-2005, continued to decrease; in 2011-2013, granite soil was used at the rate of 37%, while commercial bed soil and coir dust were used at the rates of 32% and 14%, respectively. The bulk density of commercial bed soil, expanded rice hulls, and coir dust was $0.11-0.16g/cm^{-3}$. Coir dust was the lightest material but had the highest and, 36.7% and 21.8%, respectively. In sandy loam soil and granite soil was low. The pH of all growth media was between 5.4 and 7.0, and the values were in the range of $0.15-0.66dS{\cdot}m^{-1}$. However it was necessary, to adjust the pH levels and enhance the of expanded rice hulls, sandy loam soil, and granite soil. Similarly, the fertilizer concentration and air permeability of coir dust need to be adjusted.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2022년도 추계학술대회
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• pp.129-129
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• 2022

The production area of Italian ryegrass feed is gradually increasing and labor-saving technologies are being developed. If sowing and fertilization are carried out agricultural drones, working hours and labor are reduced. The purpose of this study is to suggest an appropriate seedling amount for feed production by drone spreading of Italian ryegrass in spring. In addition, we would like to review the productivity of the drone seeding machine that is being developed by Rural Development Administration(RDA) of Korea. Italian ryegrass was sown by a drone in February at the NICS located in Gyehwa-hwa, Jeollabuk-do, South Korea. In Experiment 1, 50kg/ha, 60, 70, and 80 seeding rates were sown with a horizontal spreader drone. In Experiment 2, uniform spreaders type drone and horizontal spreader type were sown with the same seeding amount and compared. The drone was sown using the AF-52 aircraft. The higher the seeding amount, the higher the emergence rate. As the seeding amount increased, the plant length increased, but the number of tillers per individual decreased. The dry matter weight of the feed was the highest at 1,326kg/10a at the seeding rate of 70kg/ha, and decreased by 20.5% at the seeding rate of 80kg/ha. The coverage ratio was the highest at 96 at the seeding rate of 70kg/ha, which was the most advantageous for spring sowing. In the comparative experiment according to the spreader type, the uniform spreader had a high emergence rate per unit area. When the uniform spreader was used, the dry matter weight of the feed was 17% higher than that of the horizontal one, and the coverage was about 5% higher.

• 한국유기농업학회지
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• 제10권2호
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• pp.111-120
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• 2002

제주지역에서 가을 감자재배시 식물활력물질인 Chitosan 처리횟수와 감자 종묘종류가 지상부생육 및 괴경수량성 향상에 미치는 효과를 검토하였다. 공시품종으로는 제주지역 감자장려품종인 대지(Dejima)를 선정하였으며, 제주대학교 시험포(해발 280m)에서 수행하였다. 시험구 처리는 키토산 액제 2,000ppm 무처리, 1, 3, 5회 등 처리횟수를 주구, 경삽 플러그묘, 소서 플러그묘 및 일반 종서 등 감자 종묘종류를 세구로 하였다. 키토산 액제의 처리는 정식 10일 후부터 10일 간격으로 처리구에 따라 스프레이를 이용하며 엽면살포하였다. Chitosan을 3∼5회 처리함에 따라 초장 및 지상부 건물중은 유의하게 증가하는 결과를 보였다. 80g이하의 괴경수 및 괴경수량은 일반증서 파종구와 소서 플러그묘 정식구가 많았으나, 80g이상의 괴경수 및 괴경수량은 경삽플러그묘 정식구가 현저하게 많은 것으로 나타났다. 엽중의 총질소 함량은 키토산 처리횟수가 많아질수록 높아졌으며, 그 외 K, P Ca, Mg, Na 등의 함량도 유사한 경향을 보였다. 경삽 플러그묘, 소서플러그묘 정식구 및 일반증서 파종구 모두 키토산 3∼5회 처리구에서는 생육형질 및 괴경수량성에서 양호해지는 결과를 나타내었다.

• 한국산림과학회지
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• 제100권2호
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• pp.305-313
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• 2011

시비는 시설양묘과정에서 반드시 실시되어야 하지만, 토양 및 계류수 오염의 문제를 야기할 수 있다. 이에 따라 본 연구에서는 시설양묘과정에서 시비량 조절에 따른 친환경적 경제적 시비방법 구명을 위해 Constant rate, Three stage rate 및 Exponential rate 등의 3 가지 시비 처리를 실시하였으며, 시비 처리에 따른 생장 특성 및 광합성, 엽록소 형광반응, 엽록소 함량 등을 조사 분석하였다. 그 결과, 근원경, 간장 및 물질생산량은 Constant 처리에서 가장 우수하였다. 묘목품질지수 또한 같은 경향을 보였지만, 유의적 차이는 나타나지 않았다. Exponential 처리구에서도 50%의 시비량으로 규격묘 생산이 가능했다. 광합성 능력은 세 처리구 모두 우수하였으며, 광화학 효율과 엽록소 함량은 Exponential 처리구에서 유의적으로 가장 낮게 나타났다. Exponential 시비 방법으로 생리 및 생장에 문제없는 규격묘를 생산할 수 있었다. 즉, 최소 비용의 최대 생장 효과와 함께 친환경적 시비 방법이라 할 수 있다.