• Title/Summary/Keyword: Seedling production

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A Simple and Rapid Method for Functional Analysis of Plant Growth-promoting Rhizobacteria Using the Development of Cucumber Adventitious Root System

  • Bae, Yeoung-Seuk;Park, Kyung-Seok;Lee, Young-Gee;Choi, Ok-Hee
    • The Plant Pathology Journal
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    • v.23 no.3
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    • pp.223-225
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    • 2007
  • Many plant growth-promoting rhizobacteria (PGPRs) have been known for beneficial effects on plants including biological control of soilborne pathogens, induced systemic resistance to plant pathogens, phytohormone production, and improvement of nutrient and water uptake of plants. We developed a simple and rapid method for screening potential PGPR, especially phytohormone producing rhizobacteria, or for analyzing their functions in plant growth using cucumber seedling cuttings. Surface-sterilized cucumber seeds were grown in a plastic pot containing steamed vermiculite. After 7 days of cultivation, the upper part 2 cm in length of cucumber seedling, was cut and used as cucumber cuttings. The base of cutting stem was then dipped in a microcentrifuge tube containing 1.5ml of a bacterial suspension and incubated at $25^{\circ}C$ with a fluorescent light for 10 days. Number and length of developed adventitious roots from cucumber cuttings were examined. The seedling cuttings showed various responses to the isolates tested. Some isolates resulted in withering at the day of examination or in reduced number of roots developed. Several isolates stimulated initial development of adventitious roots showing more adventitious root hair number than that of untreated cuttings, while some isolate had more adventitious root hair number and longer adventitious roots than that of untreated control. Similar results were obtained from the trial with rose cuttings. Our results suggest that this bioassay method may provide a useful way for differentiating PGPR's functions involved in the development of root system.

Effects of Seedling Age on Growth and Yield of Machine Transplanted Rice in Southern Plain Region

  • Kim, Sang-Su;Kim, Bo-Kyeong;Choi, Min-Gyu;Back, Nam-Hyun;Choi, Weon-Young;Lee, Sean-Yong
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.44 no.2
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    • pp.122-128
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    • 1999
  • Three rice cultivars, Namweonbyeo (early maturing), Hwaseongbyeo (medium maturing) and Dongjinbyeo (medium-late maturing) were trans-planted with 3 different seedling ages to investigate their growth habits and to improve the cultural method in paddy fields in the southern plain area of the Honam region in 1993. The 10-day old seedlings had more vigorous elongation of plant height and higher tillering ability but lower effective tiller rate, when compared with 35-day or 40-day old seedlings. Leaf area index and top dry weight were lower in 10-day old seedlings up to 40 days after transplanting but thereafter, were not different among seedlings ages. CGR was later in 10-day old seedlings, up to 30 days after transplanting, but in 30 to 40 days after transplanting, it was reversed. RGR was the highest in infant seedlings to 40 days after trans-planting, while in 50 days after transplanting, it was reversed. Panicle number and spikelet number per square meter were the highest in 40-day old seedlings, next highest in 35-day old seedlings and the lowest and in adult seedlings up to 40 days after transplanting, while spikelet number per panicle was vice versa. Milled rice yield did not vary significantly by seedling ages, but among the varieties, it was less in Hwaseongbyeo compared with Naweonbyeo and Dongjnbyeo.

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Effects of Seed Storage Temperature and Pre-Treatment on Germination, Seedling Quality on Wild Trichosanthes kirilowii Maxim and Trichosanthes kirilowii var. japonica Kitam (종자저장온도 및 전처리가 야생 하늘타리와 노랑하늘타리의 종자발아 및 유묘 생육특성에 미치는 영향)

  • Lee, Su Gwang;Kim, Hyo Yun;Ku, Ja Jung
    • Korean Journal of Medicinal Crop Science
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    • v.22 no.1
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    • pp.53-59
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    • 2014
  • This study was conducted to determine the effects of seed storage temperature and pre-treatment on seed germination, seedling quality and vigor index of wild Trichosanthes kirilowii and Trichosanthes kirilowii var. japonica. As a result the highest germination rate of T. kirilowii was the 95% at seed stored in room temperature and then soaking for 24 hours in $dH_2O$. And the highest germination rate of T. kirilowii var. japonica was 96% at seed stored in $2^{\circ}C$ and then soaking for 24 hours in $GA_3$ (100 ppm). But the seed germination rate was non-significance in pre-treatment at seed stored in room temperature $2^{\circ}C$. Seedlings of T. kirilowii and T. kirilowii var. japonica showed not only the best seedling quality but also seedling vigor index in seed stored at $2^{\circ}C$ and then soaking for 24 hours in $GA_3$ (100 ppm). In this research, T. kirilowii and T. kirilowii var. japonica seed were stored in room temperature or $2^{\circ}C$, and then sown in peat moss, seed germination rate was more than 90% and production of superior quality seedlings.

Growth Characteristics of Ginseng Seedling Transplanting by Self Soil Nusery, Nursery or Hydroponic Culture on Main Field (토직, 상토 및 양액육묘에 의해 생산된 묘삼의 본포 이식 후 생장특성)

  • Park, Hong Woo;Song, Jeong Ho;Kwon, Ki Bum;Lee, Ueong Ho;Son, Ho Jun
    • Korean Journal of Medicinal Crop Science
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    • v.25 no.4
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    • pp.238-243
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    • 2017
  • Background: The production method of ginseng seedlings for ginseng cultivation is very important to ensure healthy rooting system as well as high quality, and yield of the resultant plants. This study was carried out to compare the growth characteristics of 2-year-old ginseng plants that were produced from seedlings grown in self soil nursery (SSN), nursery soil (NS) or hydroponic culture (HC). Methods and Results: The shading prop used was composed of four-layered 4 polyethylene (blue 3 + black 1) shade screen. The management of main field was done by inserting oil cake (1,200 kg/10 a) and then allowing Sudan grass to grow for a year. Seedling transplantation was carried out on April 6. Root growth was measured on October 25. Root weight was observed to be excellent at 6.0 g, following SSN transplantation. Root length was 21.2 cm for HC seedlings, but these plants had a physiological disorder (i.e., rusty root), in 83.5% plants of this treatment. The ratio of PD/PT (protopanaxadiol saponins / protopanaxatriol saponins) was higher in NS seedlings. Plant analysis revealed that Fe content was lower in HC seedlings with high rustiness. The growth of 2-years-old ginseng was different following these varying seedling cultivation methods, but seedlings from NS were not different from those grown in SSN. Conclusions: For the propagation of 2-year-old ginseng plants, NS seedlings may be a good substitute for SSN seedlings.

Effect of Micro Bubble on Growth of Ginseng in the shaded plastic houses and Possibility of High Quality Ginseng processing (하우스 종묘삼 재배에서 마이크로 버블(Micro bubble) 사용이 생육에 미치는 영향과 고품질 인삼 가공의 가능성)

  • Ahn, C.H.
    • Journal of Practical Agriculture & Fisheries Research
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    • v.19 no.1
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    • pp.109-117
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    • 2017
  • In the production of organic Panax ginseng, the morphological changes were confirmed by providing general water and microbubble water, respectively. Analysis of seedling ginseng treated with general water and bubbles water revealed that many seedlings were formed in the seedling treated with bubble water, and about 15% weight increase occurred in the growing period. The growth rate of stem, leaf, and root was about 15% higher than that of all. Taken together, the growth of seedling cultivation using bubble water was about 15% overall. In order to process ginseng, the dried ginseng was higher in dry weight than the general water seedling seedlings grown in bubble water. This suggests that more processed products will be produced per unit weight at the time of producing the processed products at the farm, which can directly increase the farm income.

MECHANISATION SYSTEM FOR LARGE SCALE GRAIN MAIZE PRODUCTION IN MALASIA

  • Abu-Hassan, D.;Nor, J.M.;Daham, M.D.M.
    • Proceedings of the Korean Society for Agricultural Machinery Conference
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    • 1993.10a
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    • pp.158-173
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    • 1993
  • The formulated mechanization packages for grain maize production have performed to the expected limit generating encouraging information. Besides physical feasibility , management factors viz ; production operation sequence, operations scheduling and machinery matching with respect to environment can still limit system suitability. A new production operation sequence was introduced to overcome weed problems and limitations of available working days. Proper operations scheduling will improve the initial soil-crop environment for better seedling establishment, and reduce the (). been identified as key factors to reduce capital investment and cost of proudction .

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Production of 8-ketotrichothecenes by Fusarium graminearum on Corn and Barley (옥수수와 보리에서 Fusarium graminearum의 8-ketotrichothecenes 생성)

  • 서영수;서정아;손황배;이인원
    • Korean Journal Plant Pathology
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    • v.14 no.5
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    • pp.418-424
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    • 1998
  • The production of 8-ketotrichothecenes, deoxynivalenol (DON), nivalenol (NIV), and their monoacetyl derivatives was studied in rice and corn cultures using 8 isolates of Fusarium graminearum which were obtained from corn and barley samples. Higher concentrations of trichothecenes were produced on rice than corn, and production of the toxins on rice was enhanced by growing the fungi at $25^{\circ}C$. The isolates were used for evaluation of toxin production and pathogenicity after artificial inoculation to 5 corn and 3 barley cultivars. The kinds and the relative amounts of trichothecenes produced in cultures were consistent with those in infected kernels of corn and barley with some exceptions. As for DON chemotypes, the ratios of 15-acetyl-DON to 3-acetyl-DON were varied among the pathogen-cultivar interactions. The corn and barley cultivars showed the significant differences of resistance to the Fusarium isolates in disease severity and seedling blight, and resistance ranking to the different isolates was varied. However, significant correlations were observed between the total concentrations of trichothecenes in infected kernels of corn and barley and pathogenicities of the Fusarium isolates to the hosts.

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Seedling Production and Soil Physico-Chemical Components of Nursery Field in Ginseng Plantations (농가포장(農家圃場)에서의 묘삼수량(苗蔘收量) 및 상토특성(床土特性))

  • Lee, Jong-Chul;Byen, Jeung-Su;Ahn, Dai-Jin;Kim, Kap-Sik;Park, Hoon
    • Korean Journal of Soil Science and Fertilizer
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    • v.19 no.1
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    • pp.50-55
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    • 1986
  • To get the basic information about ginseng seedling production, yields of ginseng seedling and soil physico-chemical components in 32 fields of Yang-Jik and 19 fields of Ban-Yang-Jik nursery were investigated. Germination rate is $78.5{\pm}3.0%$, $77.8{\pm}2.8%$ in Yang-Jik and Ban-Yang-Jik. Rate of number of mature seedlings to number of seeds planted is $62{\pm}13.5%$, $53{\pm}12.6%$ in Yang-Jik and Ban-Yang-Jik. Rate of number of available seedlings to number of seeds planted (Rate of available seedling) is $42{\pm}12.5%$, $26{\pm}12.1%$ in Yang-Jik and Ban-Yang-Jik. The number of available seedlings per Kan (Kan means $180{\times}90cm$ area) is $627{\pm}187$, $368{\pm}182$ in Yang-Jik and Ban-Yang-Jik. Rate of number of unusable seedlings to number of mature seedlings is 34%, 51% in Yang-Jik and Ban-Yang-Jik. It showed the negative correlation between amount of coarse sand and stem height, and central leaf length, on the other hand, possitive correlation between amount of extremely fine sand, and stem height, and central leaf length respectively. Rate of available seedling was positively correlated with amount of medium, fine and extremely fine sand, whereas negatively correlated with amount of coarse sand in Yang-Jik. Root weight per seedling was positively correlated with amount of medium, fine and extremely fine sand. Contents of organic matter, available phosphate and potassium in Ban-Yang-Jik were higher than those of Yang-Jik, but content of lime was higher in Yang-Jik compared to that of Ban-Yang-Jik. Possitive correlations were showed between leaf area per seedling and content of organic matter, and between stem height and content of lime in Yang-Jik. Root weight per seedling was positively correlated with content of organic matter and magnesium in Ban-Yang-Jik, but there is no correlation between any of soil chemical components and root weight in Yang-Jik.

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Determination of the Temperature Increasing Value of Seedling Nursery Period for Oryza2000 Model to Applicate Grid Weather Data (Oryza2000 모형 활용을 위한 육묘기 보온 상승온도 결정)

  • Kim, Junhwan;Sang, Wangyu;Shin, Pyeong;Baek, Jaekyeong;Kwon, Dongwon;Lee, Yunho;Cho, Jung-Il;Seo, Myungchul
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.22 no.1
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    • pp.20-25
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    • 2020
  • Spatial simulation of crop growth often requires application of management conditions to each cell. In particular, it is of great importance to determine the temperature conditions during the nursery period for rice seedlings, which would affect heading date projections. The objective of this study was to determine the value of TMPSB, which is the parameter of ORYZA2000 model to represent temperature increase under a plastic tunnel during the rice seedling periods. Candidate values of TMPSB including 0℃, 2℃, 5℃, 7℃ and 9℃ were used to simulate rice growth and yield. Planting dates were set from mid-April to mid-June. The simulations were performed at four sites including Cheorwon, Suwon, Seosan, and Gwangju where climate conditions at rice fields common in Korea can be represented. It was found that the TMPSB values of 0℃ and 2℃ resulted in a large variation of heading date due to low temperature occurred in mid-April. When the TMPSB value was >7℃, the variation of heading date was relatively small. Still, the TMPSB value of 5℃ resulted in the least variation of heading date for all the planting dates. Our results suggested that the TMPSB value of 5℃ would help reasonable assessment of climate change impact on rice production when high resolution gridded weather data are used as inputs to ORYZA2000 model over South Korea.

Relationship Between Yield of Seedling and Soil Physico-Chemical Components of Ban-Yang-Jik Nursery in Ginseng Plantation (산지(産地) 반양직묘포(半養直苗圃)에서의 묘삼수량(苗蔘收量)과 토양이화학성간(土壤理化學性間)의 관계조사(關係調査))

  • Lee, Jong-Chul;Byen, Jeong-Su;Ahn, Dai-Jin
    • Korean Journal of Soil Science and Fertilizer
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    • v.21 no.2
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    • pp.177-181
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    • 1988
  • To get the basic information about ginseng seedling production, yield of ginseng seedling and soil physico-chemical components of Ban-Yang-Jik (semimodified soil) nursery in 29 farmer's field were investigated. The number of available seedling per Kan (Kan means $180{\times}90cm$ area) is $362{\pm}226$. Root weight per seedling was negatively correlated with ammount of fine and extremely fine sand. Positive correlations were shown between pH and OM, K, Ca and Mg, and also between EC and Ca, Mg and ammonium and nitrate nitrogen in soil of nursery. There were significant linear relations between root weight and OM, K, Ca and Mg in soil of nursery. On the other hand, quadratic relation was held between the root weight and $P_2O_5$, but the root weight has no correlation with nitrogen. The nitrogen contents of soil might not influence on the growth of ginseng seedling as greatly as those of $P_2O_5$, K and Ca. The contents of $P_2O_5$, K and Ca in root were increased with increase of the contents of $P_2O_5$, K and Ca in soil of nusery, respectively. It showed the linear correlation between the root weight and $P_2O_5$ and Ca, otherwise quadratic correlation between the root weight and K in root.

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