• Journal of the Korean Institute of Landscape Architecture
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• v.23 no.2
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• pp.113-123
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• 1995

This study was conducted to find out appropriate rates of seed mixture of both native and foreign plants in order for stabilization of early afforestation and proper vegetation on road slope. The results are summarized as follows: 1. Characteristics of germination : After seeding, 2 weeks for both Lespedeza bicolor and Lespedeza cuneata, and 4 weeks for both Arundinella hirta and Zoysia japonica were needed for vigorous germination, and 1-4 wee17s for 3 cool-season grasses were spent for gradual germination. 2. Plant height : There was no difference between Lespedeza cuneata and Lespedeza bicolor in plant height by 7 treatments. As mixture rates of cool-season grasses were lowered, plant height of Arundinella hirta became longer. Descending order of co81-season grasses for plant height was Tall fescue, Perennial ryegrass and Creeping redfescue. 3. Number of tillers : The number of tillers tended to increase in the experimental plots where competition was low. 4 Ground cover rate : Ground cover rate was the highest in Mixture IV (96.7%) and was fluctuated from 13.3% on Sept. 13 to 45% on Nev. 3 in Mixture III. Descending order of gronud cover rates in 7 treaments was Mixture IV, Mixture III. Mixture II, Mixture Ⅶ, Mixture V, Mixture Ⅵ, Mixture I, and Mixtur III when measured on Oct. 13 5. Visual assessment: High preferences were observed on Mixture IV and Ⅶ In sun the best seed mixtures were Mixture IV and Ⅶ. It meant that (1) either mixture of 70% the native plants with 30% cool-season graaes or (2) mixture of 80% the native plants with 20% cool-season glasses was best for this study.

• Horticultural Science & Technology
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• v.34 no.3
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• pp.363-371
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• 2016

Tabulis [Astilboides tabularis (Hemsl.) Engl.] has the potential for becoming a useful horticultural crop. This study was conducted to classify the seed dormancy types and to identify the germination conditions that improve the horticultural usefulness of this plant. We found that A. tabularis (Hemsl.) Engl. produced undifferentiated embryos and had a low germination rate of under 50%. In addition, water submersion led to moisture absorption. Therefore, A. tabularis seeds were designated as morphologically dormant. The germination rate was highest (86.3%) at $30^{\circ}C$ under light conditions. Higher temperatures generally led to an acceleration of the germination process, regardless of light condition. To improve the germination rate, seeds were submerged in various concentrations of growth regulators, such as $GA_3$ and kinetin, as well as minerals such as $KNO_3$ and KCl. An analysis of germination characteristics at $30^{\circ}C$ under light conditions revealed that treatment with 200 and $500mg{\cdot}L^{-1}GA_3$ led to an excellent germination rate of 97.0%. Treatment with $100mg{\cdot}L^{-1}GA_3$ led to a better germination rate than the control as well. However, treatment with kinetin, $KNO_3$, and KCl had no effect on germination, regardless of concentration.

• International Journal of Industrial Entomology and Biomaterials
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• v.8 no.2
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• pp.201-205
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• 2004

During the routine survey, the mortality of mulberry seedlings was noticed due to damping-off disease. The disease recognized by rotting of emerged seedlings near the soil line (just below the soil level) resulting in collapse of the seedlings. Two fungi were isolated from affected samples and identified as Alternaria alternata (Fr.) Keissler and Fusarium solani (Mart.) Sacc. Both the fungi were found to be responsible in causing pre and post emergence damping-off of seedlings in mulberry. For management of the disease, an experiment was conducted using fungicides. These fungicides were applied as seed treatment; soil drenching and foliar spray alone and in combination. Among the different treatments, integration of seed treatment and soil application of Dithane M-45 (Mancozeb 75% WP) + Bavistin (Carbendazim 50% WP) followed by foliar spray of these fungicides (after 35 days of sowing) resulted in better survivability of seedlings (93.3 %) on $90^th$ day and controlled the pre and post emergence damping off by 100 and 89.5%, respectively over the check.

• Journal of Microbiology and Biotechnology
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• v.30 no.12
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• pp.1854-1861
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• 2020

Staphylococcus aureus is one of the most common microorganisms and causes foodborne diseases. In particular, biofilm-forming S. aureus is more resistant to antimicrobial agents and sanitizing treatments than planktonic cells. Therefore, this study aimed to investigate the anti-biofilm effects of cell-free supernatant (CFS) of Saccharomyces cerevisiae isolated from cucumber jangajji compared to grapefruit seed extract (GSE). CFS and GSE inhibited and degraded S. aureus biofilms. The adhesion ability, auto-aggregation, and exopolysaccharide production of CFS-treated S. aureus, compared to those of the control, were significantly decreased. Moreover, biofilm-related gene expression was altered upon CFS treatment. Scanning electron microscopy images confirmed that CFS exerted anti-biofilm effects against S. aureus. Therefore, these results suggest that S. cerevisiae CFS has anti-biofilm potential against S. aureus strains.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.47 no.6
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• pp.432-436
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• 2002

This study was conducted in 2001 to determine the influence of seed-piece size (SPS) and number (SPN) per mini-tuber produced by hydroponics on growth and yield of potato (Solanum tuberosum L.). Treatments consisted of the combination of six SPS (3, 5, 7, 9, 11, 13g/seed-piece) and four SPN (whole tuber, and two, three, and four pieces/tuber). Percentage sprout emergence at transplanting was higher for seed pieces weighing 7 to 13 g (83.0 to 85.4%) and for three and four pieces per tuber (85.4 and 84.8%). As SPS were increased from 3 to 9 g/piece, total yields markedly increased from 2,052 to 2,924 kg/10a and seed tuber (31 to 250 g) yield from 1,589 to 2,428kg/10a with no further increase with SPS. As SPN was increased from one to three/ tuber, total yields increased from 1,918 to 2,982 kg/10a and seed tuber yields from 1,422 to 2,579 kg/10a with no further increase with SPN. Average tuber weight increased with SPS (46.3 to 71.5 g/tuber) and SPN (56.5 to 64.9 g/tuber).

• Journal of Korean Society of Forest Science
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• v.87 no.4
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• pp.549-556
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• 1998

To develop the effective methods of flowering stimulation, Hinoki cypress (Chamaecyparis obtusa Sieh. et Zuec.) grafts growing in a seed orchard and in a clone bank in Southern Breeding Station of Cheju were applied with gibberellin treatments, and predicted the seed production potential. In the seed orchard, $GA_{4/7}$ 1.5cc was injected into the stem of drafts and sprayed whale tree crown with $GA_3$ 300ppm and $GA_{4/7}$ 300ppm. Un the other hand, in the clonal archives, drafts were given intrusion of $GA_{4/7}$ 1.5cc into the excised and open inner part of bark wind $GA_3$ 20mg and sprayed with $GA_3$ 300ppm. Additionally, grafts growing in the seed orchard were treated with gibberellins at 3 different periods of time and 3 different treatments during the growing season. The results obtained here are summarized as follows : 1. All of the applications of Gilbberellin promoted female flower formation. Among these, the treatment of intrusion of $GA_{4/7}$ 1.5cc into the excised and open inner part of bark was racist effective, followed by the spraying of $GA_3$ 300ppm. Similarly, the applications of gibberellin promoted male flower formation. 2. Regarding the time of applications, treatment on August 15 was more effective than those of August 31 and September 11 in the stimulation of female flowers. On the contrary, there was no significant difference in the number of male flowers among 3 different time treatments. 3. It was supposed that the application of the intrusion of $GA_{4/7}$ 1.5cc into the excised and open inner part of bark on August 15 showing the best effect in female flower formation can produce 22.12kg seeds per ha. 4. Considerable significant difference existed among clones for both female and male flower formations. 5. Flower formation, especially female flower formation, seemed to be partially associated with the genetic potential of individual trees.

• Journal of Life Science
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• v.13 no.1
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• pp.9-14
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• 2003

Seed germinability might be highly related to seedling establishment. The experiments were carried out to evaluate the effect of presowing treatments of aging, priming, $GA_3$ , prechilling and water imbibition, different temperatures and light quality during germination on germination of gourd (Lagenaria siceraria Standl.) seeds. Aging treatment with different temperatures and periods was done by the accelerated aging method. Priming using KNO$_3$ and $GA_3$ treatment for 24 hours were done at 100 mM and at 0.01 mM before a week prechilling. The germination tests using a week prechilled seeds were done at 10, 20 and $30^{\circ}C$ on the condition of darkness, and also with blue, red, far-red light and darkness as light quality treatments. FR-yongjadaemok and FR-kunghap were used as test cultivars and their daily germination rates were measured at treatment levels. Germination rates were reduced in the seeds aged at $45^{\circ}C$ for 6 days. Prechilling showed the highest and fastest rate among the four presowing treatments but the two other presowing treatments had similar or less rates than water imbibition. Prechilling done before sowing enhanced the low vigor seeds, and accelerated the germination of cv. FR-kunghap at 20 to $30^{\circ}C$ and with blue, red light or darkness during germination although far-red light inhibited their germination.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.39 no.6
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• pp.594-601
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• 1994

Effects of translocation on seed yield and carbon-nitrogen compounds at five leaf-pod ratios of soybean [Glycine max. (L.)Merr. ] culti bars, 'Paldalkong', 'Baekunkong', and 'Danyeobkong' were measured. The upper 50 and lower 50% of leaves and pods were subjected to treatments at growth stage R3. Three soybean cultivars showed the similar trends on changes in dry matter accumulation and on the contents of soluble sugar, starch and protein in seeds among the treatments. Mean stem dry weight was increased with upper leaf-lower pod and lower leaf-upper pod removals, and decreased with upper leaf and lower leaf removals. Leaf dry weight was appeared higher at the upper leaves among the treatments. Seed numbers and dry weights were decreased with leaf and leaf-pod removals, and were higher in lower part of the plants. Soluble sugar and starch contents in seeds were also showed slightly higher in lower part. Protein content of seeds was decreased in upper part with upper leaf removal and in lower part with lower leaf removal, however, that of the upper seeds was the highest with lower leaf removal. The results of this study are assumed that carbon and nitrogen compounds were translocated opposite directions and protein source was weak in remobilization for the long distance transport during the reproductive growth period of soybean plants.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.64 no.1
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• pp.18-24
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• 2019

Isoflavones, a group of secondary metabolites present in plants and especially abundant in soybeans, are garnering increasing interest because of their medicinal properties. Numerous environmental factors influence isoflavone accumulation in plants. Successful attempts to increase isoflavone contents in soybean seeds have not been widely reported. In this report, we examined an effective method for producing a high amount of isoflavones during soybean seed germination, by treating the seed with 4 abiotic factors. Soybean seeds were exposed to ultrasound (50 Hz) and heat ($40^{\circ}C$) for 30minutes, then germinated for 5 days in darkness. Another sample was subjected to water deficit during germination in darkness, and the other sample was subjected to light conditions during the germination period. The results showed that the ultrasound treatment increased isoflavone content during soybean germination. Three days after germination, the ultrasound-treated seeds presented the highest isoflavone content (6,042.1 ug/g dry weight) compared to the other treatments, which was also 1.3-fold increases over the content of the raw seed's before germination. With respect to each type of isoflavone group, aglycone (partially genistein) content was markedly higher, whereas glucoside was lower than that of the untreated samples. In other experiment, total isoflavone content after heat, water deficit, and light treatments was 5,600.0, 4,740.1, and 5,631.4 ug/g, respectively. These present work suggests that ultrasound treatment is a novel approach for improving the production of isoflavones in soybean seeds during germination.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.18 no.4
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• pp.1-14
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• 2015

A manipulation of zoysiagrass's lateral stems and soil-media; used for slope revegetation, is expected to facilitate the production of high-quality grass. To study the influences on the growth of zoysiagrass depending upon various soil-media conditions, two different types of soil are used. The results obtained - through investigation of its cover rates, leaf color and number - are summarized as follows. In mountain regions soil, there are no significant differences in growth and development of grass in treatments: zoysiagrass's lateral stems treatments with 1cm, 2cm, and 3cm soil-media and treatment with only seeding. Zoysiagrass, in most of the treatments, show about the same growth rates, and at the end, fair visual quality. Zoysiagrass's lateral stems treatments with 2cm, 3cm show slightly better growth, however, thickness of soil-media need not be more than 1cm to obtain an expected quality of lawn. In decomposed granite soil, there appears statistical significance in growth of the grass in treatments: zoysiagrass's lateral stems treatments with 1cm, 2cm, and 3cm soil-media and treatment with only seeding. The thicker the soil-media, the better the growth of grass, and that in treatment with seeding-only shows poor quality in general. And therefore, it is efficacious to plant in 3cm soil-media when quick formation of lawn is necessary; however, using 2cm soil-media is ultimately the most cost-efficient way of formation. But, when time allows - that is, more than three months - 1cm soil-media in decomposed granite soil is reasonable to formate just as effective lawn. And so when performing seeding, additional covering, fertilization or increasing the quantity of seed must be considered.