• Journal of Food Hygiene and Safety
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• v.38 no.4
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• pp.264-272
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• 2023

The aim of this study was to compare the antioxidant activities and functional contents of Korean conventional and Chinese seed gingers from the Jeollabuk-do Wanju and Chungcheongnam-do Seosan regions. Ginger samples were subjected to steaming treatments for different durations (2-8 h) at 121℃ using an autoclave. The antioxidant activity was evaluated by measuring total polyphenol and flavonoid contents and ABTS and DPPH radical scavenging activities, while functional ingredient contents were analyzed for gingerols and shogaols. The results showed that Wanju conventional seed ginger (WO-2) had the highest total polyphenol (85.24 mg GAE/g) and flavonoid (98.14 RE/100 g) contents, surpassing that of the control in all steamed groups at 6 h. ABTS radical scavenging activity showed a strong correlation with total polyphenol and flavonoid contents. The control groups indicated that Korean conventional seed ginger had 1.0-1.3 times higher gingerol contents compared to Chinese seed ginger. Furthermore, the content of shogaols, considered major functional ingredients, increased significantly with longer steaming durations, reaching the highest content (1,793 mg/kg) at 8 h, which was 1.0-1.8 times higher in Korean conventional seed ginger than that in Chinese seed ginger. These experiments provide valuable data supporting the excellence of Korean conventional seed ginger in the future.

• Journal of Bio-Environment Control
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• v.32 no.3
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• pp.210-216
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• 2023

Conventionally, the seeds of Rehmannia glutinosa (Gaertn.) Libosch. ex Steud. have been directly sown at the field without using the plug seedling method. Plug seedlings have the advantage of promoting germination and convenient transplanting. However, there is little information about propagation of R. glutinosa using the plug seedling method. This study was conducted to investigate the optimal seed rhizome length, diameter, and sowing direction of R. glutinosa for establishing the plug seedling method. Seed rhizome length and diameter were separated by 1, 2, 3 cm and 0.3-0.5, 0.6-1.0, 1.1-1.5 cm, respectively. And seed rhizomes were sown in vertical and horizontal directions. The survival rate in 1 cm length of seed rhizomes was lower than in other treatments. The leaf length, leaf width, number of leaves, SPAD, leaf area, and fresh and dry weights of shoot and roots were the greatest in 3 cm length of seed rhizomes. As the seed rhizome diameter decreased, the growth characteristics of R. glutinosa tended to increase. When R. glutinosa was sown horizontal direction, the leaf length, leaf width, number of leaves, and leaf area were significantly higher than in the vertical direction. In conclusion, when sowing seed rhizome in a plug tray, using a length of 3 cm, a diameter of less than 1 cm, and sowing in a horizontal direction is considered an appropriate sowing method for R. glutinosa.

• Korean Journal of Medicinal Crop Science
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• v.26 no.1
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• pp.55-63
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• 2018

Background: The present study assessed the response of kenaf (Hibiscus cannabinus L., Jangdae) seed to NaCl and the effects of polyethylene glycol (PEG) on kenaf seed germination and vigor. Methods and Results: Seed germination ranged from 11.3% to 58.8% after 24 hours of immersion in NaCl concentrations from 0% to 0.5%. The priming treatments had lower electrical conductivity (EC) values for the seeds than for the control and a deteriorated palisade layer. Priming in 10% PEG for 48 hours increased the germination upto 96.3% in $H_2O$ solution and 98.8% in 0.3% NaCl solution compared to that of the control (78.8%). Germination synchronization, and shoot and root growth of the primed seeds were greater than those of the control. The $T_{50}$ of the control in $H_2O$ and 0.3% NaCl solution was 22 and 28 times, respectively. After priming, nine times was sufficient to reach $T_{50}$ in both solution. The mean number of days to germination (MDG) decreased from 1.43 days for the control to 0.55 days for 0% PEG in $H_2O$ solution and from 1.57 days for the control to 0.56 days for 0% PEG in 0.3% NaCl solution. The dry weight after the 10% PEG treatment was higher than that of the control. Conclusions: Taken together, 10% PEG treatment for 24 hours is recommended for kenaf seed invigoration before planting.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.52 no.2
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• pp.153-161
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• 2007

Field experiments were conducted to determine the physiological and biochemical basis of the interactive effect of sulphur (S) and nitrogen (N) application on seed and xanthotoxin yield of Ammi majus L. Six treatments were tested ($T_1$ = control-without manure and fertilizers, $T_2$ = manure @ 9 kg $plot^{-1}-10\;t\;ha^{-1},\;T_3=A_0N_{50}K_{25}P_{25},\;T_4=S_{40}N_{50}K_{25}P_{25},\;T_5=S_{40}N_{100}K_{25}P_{25}\;T_6=S_{20+20}N_{50+50}K_{25}P_{25})$). Nitrate reductase (NR) activity and ATP-sulphurylase activity in the leaves were measured at various phonological stages, as the two enzymes catalyze rate-limiting steps of the assimilatory pathways of nitrate and sulphate, respectively. The activities of these two enzymes were strongly correlated with seed and xanthotoxin yield. The highest nitrate reductase activity, ATP-sulphurylase activity and xanthotoxin yield were achieved with the treatment $T_4$. Any variation from this treatment decreased the activity of these enzymes, resulting in a reduction of the seed and xanthotoxin yield in Ammi majus L. The higher seed and xanthotoxin yield achieved in Ammi majus L. at treatment $T_4$ could be due to optimization of leaf soluble protein and photosynthetic rate, as these parameters are Influenced by S and N assimilation.

• Korean Journal of Plant Resources
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• v.22 no.3
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• pp.236-241
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• 2009

Dracocephalum argunense Fischer ex Link (Labiatae) is a perennial herbaceous plant used as valuable materials for ornamentals, honey production, and pharmaceutics. Since seed germination of this species was quite difficult, present studies were conducted to improve the germination rate by subjecting the seeds to various environmental conditions (temperature and light) and treatments (scarification, priming and seed coating). Optimum temperature for adequate germination was 20$^{\circ}C$ though it ranged from 15$^{\circ}C$ to 25$^{\circ}C$, and low temperature treatment improved germination rate. Light was required for higher germination rate in this species. The scarification of seeds resulted in much higher germination, especially by the physical treatment with sandpaper or chemical treatment with sulfuric acid for 30 seconds. Various primers with different concentrations were treated on the seeds and it was demonstrated that low temperature enhanced germination rate, regardless of kinds and concentrations of the primers. Three treatment combinations of the primers, 0.5 mM $GA_{3}$ treated for 48 hours, 0.5 mM IAA for 24 hours, and 1.0 mM IAA for 24 hours, increased the seed germination rate profoundly. Soaking treatment of inorganic salts, $KNO_{3}$ and $KH_{3}PO_{4}$, promoted germination when seeds were subjected to low temperature. Water soluble primers such as sucrose at 0.5 and 3% concentration and solid primer talc powder were effective in enhancing germination rate.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.41 no.3
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• pp.384-394
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• 1996

The experiment was conducted to determine the seed characteristics and preferable methods to enhance the seed germination rate in Bupleurum falcatum. The optimum temperature for the seed germination of Bupleurum falcatum is 2$0^{\circ}C$. Any significant promoting effects were not found in seed germination with hormone treatments and physical methods. At 15$^{\circ}C$, prechilling combined with 50~200ppm of GA$_3$ treatment raised germination rate by 2 times of control ones. The most positive effect was observed in the treatment of 10$^{-2}$ ~10$^{-3}$ M potassium nitrate only at 15$^{\circ}C$ for 12 and 24 hours. The leachate of Bupleurum falcatum didn't inhibit the germination of Lactuca sativa, showing almost 100% of germination rate, which is suggested that no inhibitors contained in the seeds of Bupleurum falcatum. Observation of embryo conditions under stereoscopic microscope showed that the ratio of seeds with or without embryo is almost 50/50. The results suggested that the lower rate of germination in Bupleurum falcatum was caused by embryolessness of seeds.

• Korean Journal of Organic Agriculture
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• v.20 no.4
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• pp.589-605
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• 2012

This study was conducted to identify allelopathic effect of Ganghwa domestic Artemisia spp., named Sajabalssuk and Ssajuarissuk, for various receptor plants including clover (Trifolium repens L.), alfalfa (Medicago sativa L.), lawn grass (Zoysia japonica Steud.), dandelion (Taraxacum platycarpum Dahlst.), and dahurianpatrinia (Patrinia scabiosaefolia Fisch. ex Trevir). Receptor plants were treated with the aqueous and essential oil extract of Artemisia plants. In consequence, their allelopathic effects were evaluated by measuring seed germination rates, seedling growth, and dry weights of the receptor plants. The seed germination and seedling growth of the receptor plants were inhibited by all treatments of both aqueous and essential oil extracts of the Artemisia plants, and, in addition, the inhibitory effects were increased according to the higher concentration. Among the donor plants, A. $sp.^*III$ showed most effective allelopathic effect. Comparing the alleopathic effect among the receptor plants, seed germination was most inhibited in lawn grass while inhibitory effect of seedling growth was comparatively higher in dandelion. Although inhibitory effects were comparatively lower, the allelopathic effects of Artemisia plants were identified in clover and alfalfa since the seedling growth of these plants were inhibited more than 70%. Thus, in result, Ganghwa domestic Artemisia spp. could be possibly used for weed control since natural products of the plants showed inhibitory effects on seed germination and seedling growth of various receptor plants.

• Korean journal of food and cookery science
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• v.14 no.4
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• pp.380-387
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• 1998

The stability of red pepper seed oils during storage at 20${\pm}$3$^{\circ}C$, 40${\pm}$3$^{\circ}C$ and heating at 140${\pm}$3$^{\circ}C$ or 180${\pm}$3$^{\circ}C$ were measured to evaluate red pepper seed oil as a cooking oil. Two species of red pepper seeds (native, improved) were dried by either sunlight or heating to prepare the oil samples of NS (native, sunlight-dried), IS (improved, sunlight-dried), NF (native, heated), and IF (improved, heated). During storage at 20${\pm}$3$^{\circ}C$ or 40${\pm}$ 3$^{\circ}C$, acid values (AV) of all red pepper seed oils were higher than that of soy bean oil (SBO), however, peroxide values (POV) were similar to SBO. Antioxidative stability of NS was better than SBO but IF was not. By the heat treatments at 140${\pm}$3$^{\circ}C$ or 180${\pm}$3$^{\circ}C$, NS was identified to have better antioxidative stability than SBO and IF was the lowest. In sensory evaluation of each deep-fat fried potato-chip at 180${\pm}$5$^{\circ}C$, potato-chips fried in NS were better than that of SBO for color, taste, and flavor. NS-fried potato-chips got the highest score in overall acceptance (p<0.05), however, those of IF showed little acceptance. When blended oils (SBO: NOS, 0, 25, 50, 75%) were used, 50% blended oil was the best for taste, color, flavor, cripness, and total acceptance.

• The Plant Pathology Journal
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• v.16 no.6
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• pp.312-317
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• 2000

The selected five plant growth-promoting rhizobacteria (PGPR) strains, WR8-3 (Pseudomonas fluorescens), WR8-6 (P. putida), WR9-9 (P. fluorescens), WR9-11 (Pseudomonas sp.), and WR9-16 (P. putida) isolated in the rhizosphere of watermelon plants were tested on their growth promotion and control effect against gummy stem rot of watermelon. Strains, WR8-3 and WR9-16 significantly increased stem length of watermelon, and there was a little increase in leaf area, fresh weight and root length when strains, WR8-3, WR9-9 and WR9-16 were treated. Generally, seed treatment was better for plant growth promotion than the soil drench, but there was no significant difference. Seed treatment and soil drench of each bacterial strain also significantly reduced the mean lesion area (MLA) by gummy stem rot, but there was no significant difference between the two treatments. At initial inoculum densities of each strain ranging from 10$^6\;to\;10^{15}$ cfu/g seed, approximately the same level of disease resistance was induced. But resistance induction was not induced at the initial inoculum density of 10$^3$ cfu/g seed. Resistance was induced by treating the strains, WR9-9, WR9-11 and WR9-16, on all of four watermelon varieties tested, and there was no significant difference in the decrease of gummy stem rot among varieties. Populations of the strains treated initially at log 9-10 cfu/g seed, followed with a rapid decrease from planting day to 1 week after planting, but the population density was maintained above log 5.0 cfu/g soil until 4 weeks after planting. Generally no or very weak in vitro antagonism was observed at the strains treated excepting WR9-11. Rifampicin-resistant bacteria which had been inoculated were not detected in the stems or leaves, which suggesting that the bacterium and the pathogens remained spatially separated during the experiment. This is the first report of rsistance induction in watermelon to gummy stem rot by PGPR strains.

• Korean journal of applied entomology
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• v.26 no.2 s.71
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• pp.107-111
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• 1987

Experiments were caried out to invesstigate the effects of seed disinfectant and the chemical control method in the paddy field for the rice white-tip nematode, Aphelenchoides besseyi. All three seed disinfectants tested, MEP 50% EC, Fenthion 50% EC, Dasuzin 34% EC showed effective control without any symptom of phytotoxicity. Mixed treatments with fungicides, Benoram 40% Wp, proraz-25% EC, TCM 30% EC also showed the effective control and no phytotoxicity. Effective chemical control methods for the rice white-tip nematode in paddy fields was seed disinfectant before seeding and Carbofuran 3% G. on the day before the transplanting, or seed disinfectatnt + Carbofuran 3% G. water surface treatment on the early stage of injury.