• Title/Summary/Keyword: Seed treatment

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Physical Seed Treatment Techniques for Germination Enrichment and Seed Sterilization (발아증진 및 소독을 위한 물리적 방법을 이용한 종자처리 기술)

  • Si-Yong Kang
    • Journal of Radiation Industry
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    • v.17 no.2
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    • pp.199-207
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    • 2023
  • Since seeds can be directly used as food resources as well as for crop cultivation or preservation of genetic resources, it is essential to develop high-quality seed processing technology to increase agricultural productivity. Seed treatment means processing technologies of seeds through physical or chemical treatment processes from after harvesting seeds to before sowing of seeds to improve germination and growth rate, durability, and immunity, etc. Since chemical seed treatment technology using pesticides or plant growth regulators has problems of environmental pollution and human toxicity, it is desired to develop an alternative technology. As a physical seed treatment method, various technologies such as ionizing radiation, plasma, microwave, and magnetic field are being developed, and some of them are being used practically. In this paper, I will summarize the mechanism of seed priming and disinfection, and the advantages and disadvantages of application, focusing on these physical seed treatment methods. Low dose or moderate intensity ionizing radiation, microwave, low-temperature plasma, and magnetic field treatments often promoted seed germination and seedling growth. However, effective removal of direct seed pathogens at these treatment intensities appears to be difficult. And it has been shown that relatively high-dose electron beam treatment using low-energy electron beams kills microorganisms on the seed surface and hull layer while not damaging the inner tissue of the seed, and is also effectively used for seed treatment on a commercial scale. In order to put the physical seed treatment technology to practical use in Korea, it is necessary to develop an economical scale treatment device along with the development of individual treatment technology to each crop.

Studies on Seed Germination of Miscanthus sinensis Native to Jeju Island (제주도 자생 참억새의 종자발아에 관한 연구)

  • Lee, Jong Suk;Han, Seung Won
    • Journal of the Korean Society of Environmental Restoration Technology
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    • v.10 no.1
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    • pp.9-15
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    • 2007
  • The study was conducted to find out the harvesting time, storage duration, cold treatment and sterilization on seed germination of Miscanthus sinensis. The optimum harvesting time of the Miscanthus seed in native to Jeju island was December. And the seed germination passible 3 years after harvesting. When the seeds had cold treatment, seed germination nate was 74% rather than 63% of non-treatment. For seed sterilization treatment, soaking in solution of Benomyl 7% during from 3 hours to 12 hours germinated 9% over. But the non-treatment on 24 hours treatment reduced the seed germination of Miscanthus sinensis.

The Effect of Seed Coat Scarification with Sodium Hypochiorite on Germination of Zoysiagrass Seed (Zoysiagrass 종자 발아에 미치는 Sodiym Hypochlorite의 종피처리 효과)

  • 구자형;김태일;원동찬
    • Asian Journal of Turfgrass Science
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    • v.3 no.2
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    • pp.89-94
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    • 1989
  • Research was conducted to obtain t he optimum treatment Of sodium hypochlorite(NaOC I) at various temperatures in t he seed scarification for stimulating germination of zoysiagrass (Zocysia Japonica Steud ) seed. Morphological changes of seed coat were also examined by scanning electron micros cop(SEM). l. Differences in temperature of scarification with 2 .4% NaOCI showed little influence on promoting germination of seeds but seeds treated with 1% solution at l5˚C germinated less than that of higher temperatures. The promotion effect of 4% solution on germination was diminished when seeds were treated for 8 hours of more. The most favorable seed scarification unaffected hy temperature for enhancing germination was 4-6hours treatment at 4% solution in fresh seeds. 2. $GA_3$, treatment did not enhance germination of water-pretreated control seeds hut germination of seeds pretreated with NaOCI l was increased additional 10% or more hy$ GA_3$, Water pretreated control seeds treated with 50 mM hydrogen peroxide(H'O )germinated about 44%. In NaOCI treated seeds. $H_2$$O_2$ treatment increased germination additional l 0% or more. 3. NaOCI l and KOH treatment softened the seed coat and formed the pores hy removing the scab-like thikenings attached to the seed surface. These results suggest that the modes of action of NaOCl in the promotion of seed germination reside in it increase of the permeability of the seed coat, and in the provision of additional oxygen to the seed.

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The Effects of Some Environmental Factors on the Seed Germination of Albizzia julibrissin Durazz. (藪種 環境要因이 자귀나무의 種子發芽에 미치는 影響)

  • Lee, Ho Joon;Seon Ho Kim;Hae Won Kang
    • The Korean Journal of Ecology
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    • v.8 no.2
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    • pp.69-73
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    • 1985
  • Germination respone to sulfuric acid treatment, temperature, light and underwater condition were experimented for Albizzia julibrissin Durazz. seed. It took 60 minutes to break the dormancy of impermeable seed for the effective sulfuric acid treatment, and temperature sensitivity was decreased by 90 minutes' treatment. The germination rate of the seed was highest, i.e. 96% at 60 minutes acid treatment in a 21。C growth chamber. On the occasion of light sensitivity, the seed was light indifferent. The imbibition rate of seed was higher at 27。C than 21。C and in proportion to the period of acid treatment time. A. julibrissin Durazz. seed were well germinated at underwagter condition.

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Yield and Seed Quality as Affected by Water Deficit at Different Reproductive Growth Stages in Soybean

  • Kim, Wook-Han;Hong, Byung-Hee;Kim, Seok-Dong
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.44 no.4
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    • pp.321-329
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    • 1999
  • The effect of water deficits on soybean [Glycine max (L.) Merr.] could appear on seed quality through changes of morphological plant characteristics. Two Korean genotypes, Hwangkeum (determinate growth habit) and Muhan (indeterminate growth habit), were used to examine the influences of treatment stage and method of water deficit during reproductive growth period on yield and seed quality of soybean. Water deficit at R5 or R6 stages was as damaging to seed quality as double water-deficit treatments at R2+R5 or R2+R6. However, seed from double water-deficit treatment tended to have lower oxidation-reduction potential compare to the corresponding single water-deficit treatment. In comparison with Muhan, Hwangkeum had significantly greater oxidation-reduction potential value. Seed yield per plant in both genotypes depended greatly on seed yield of branches. However, the proportion of number of branch seed to total seed umber in Hwangkeum was increased as the water deficit was applied during later reproductive stage, whereas, in Muhan the proportion was lower. Water-deficit treatments including the single and double water-deficit treatments and non-stressed treatment were able to be classified into five groups for Hwangkeum and four groups for Muhan based on the influences on yield components, number of pod, number of seed, and single seed weight, using principal component analysis. In both genotypes, R2+R5 water-deficit treatment decreased number of pod and seed, but increased single seed weight. On the contrary, R6 or R2+R6 stress increased the pod and seed number, but decreased single seed weight.

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Comparison of Sodium Hypochiorite and Potassium Hydroxide as Seed Treatment for Stimulating Germination of Korean Lawngrass (Zoysia japonica Steud.) Seed (한국잔디종자의 발아촉진을 위한 Sodium Hypochlorite와 Potassium Hydroxide 처리효과의 비교)

  • 구자형;원동찬;김태일
    • Asian Journal of Turfgrass Science
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    • v.2 no.1
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    • pp.41-48
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    • 1988
  • Research was conducted to obtain easy and secure methods for promoting germination of Korean lawngrass (Zoysia japonic a Steud.) seed. The effect of sodium hypochiorite (NaOCl) treatment on germination of seeds was compared with that of potassium hydroxide (KOH) treatment under conditions of duration of seed storage after harvest and temperature of treatment. Emergence of seedlings from soil and micro-flora infection rate among seed treatments were investigated. 1. The promotion of germination was much more evident in seeds treated with 4% NaOCl for 8 and 10 hours than in seeds treated with 25% or 30% KOH for 30 and 40 minutes. Longer durations of storage before seed treatment in KOH than in NaOCl were required to initiate enough germination. 2. Differences in temperature of treatment with NaOCT did not have much influence on germination of seeds and treatment temperature of 15˚C was better than that of 200˚C and 300˚C for promoting germination. But seed treatment with KOH significantly promoted germination with increasing temperature of treatment from I 5˚C to 20˚C and 30˚C. 3. GA$_3$ treatment enhanced germination in NaOCl-pretreated seeds at early stage of imbibition and in-creased about 10% germination after 10 days of imbibition in KOH-pretreated seeds. 4. NaOC1 treatments significantly decreased the rate of the infection of microflora in seed samples and enhaced emergence of seedlings from soil compared with KOH treatment. 5. NaOC1 treatment had advantage over KOH treatment with respect to ease of preparing, securing and handling in stimulating germinating of Korean lawngrass seeds.

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Improvement of Electrodeposition Rate of Cu Layer by Heat Treatment of Electroless Cu Seed Layer (Cu Seed Layer의 열처리에 따른 전해동도금 전착속도 개선)

  • Kwon, Byungkoog;Shin, Dong-Myeong;Kim, Hyung Kook;Hwang, Yoon-Hwae
    • Korean Journal of Materials Research
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    • v.24 no.4
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    • pp.186-193
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    • 2014
  • A thin Cu seed layer for electroplating has been employed for decades in the miniaturization and integration of printed circuit board (PCB), however many problems are still caused by the thin Cu seed layer, e.g., open circuit faults in PCB, dimple defects, low conductivity, and etc. Here, we studied the effect of heat treatment of the thin Cu seed layer on the deposition rate of electroplated Cu. We investigated the heat-treatment effect on the crystallite size, morphology, electrical properties, and electrodeposition thickness by X-ray diffraction (XRD), atomic force microscope (AFM), four point probe (FPP), and scanning electron microscope (SEM) measurements, respectively. The results showed that post heat treatment of the thin Cu seed layer could improve surface roughness as well as electrical conductivity. Moreover, the deposition rate of electroplated Cu was improved about 148% by heat treatment of the Cu seed layer, indicating that the enhanced electrical conductivity and surface roughness accelerated the formation of Cu nuclei during electroplating. We also confirmed that the electrodeposition rate in the via filling process was also accelerated by heat-treating the Cu seed layer.

Studies on Establishment of Oversown Pasture Seed I. Effects of coating materials and minerals on germination (겉뿌린 목초종자의 정착에 관한 연구 I. 각종 증량재 및 미량광물질의 종자피복이 발아에 미치는 영향)

  • 이효원;정병용;김희경
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.7 no.2
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    • pp.113-119
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    • 1987
  • This experiment was carried out in laboratory to find out the effect of coating materials, minerals and insecticides on germination of pasture seed. Seed coating was made by specially made machine and seed germination in petridishes was also determined from Sep. 1986 to May 1987. The results were summarized as follows; 1. Coating seed gave bad germination compared with the control. There were significant difference between treatment, coating materials and adhesives in white and red clover while Phleum pratense germination was improved by coating materials. 2. In mineral coating treatments, on Mg alone or Mg mixed treatment, germination was deepressed. Generally germination was lowed by mineral treatment, but the difference was small. 3. Uncoating treatment with insecticide was superior to coating treatment in terms of germination. Among the insecticide Ballen gave more serious effect to seed germination.

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Seed Dormancy and Germination Characteristics of Endemic Elder Species (Sambucus racemosa subsp. pendula) and Common Elder Species (S. williamsii) in Korea

  • Hyo-In, Lim
    • Journal of Forest and Environmental Science
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    • v.38 no.4
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    • pp.284-289
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    • 2022
  • This study was conducted to determine the seed dormancy types of Sambucus racemosa subsp. pendula Nakai and S. williamsii Hance. Low temperature stratification (1, 2, 3, 4 months) and GA3 treatment (1,000 mg/L) were performed on seeds to determine the type of seed dormancy. After the treatment, seeds were placed on a petri dish at 25℃ under light conditions. The germination rate and mean germination time were investigated. Results showed that cold stratification was effective in breaking the dormant state of the seed in both species. In the low temperature stratification treatment, the seed germination performance was improved as the treatment period was prolonged. Gibberellin treatment was effective in breaking the dormant state of S. racemosa subsp. pendula without low temperature stratification. However, S. williamsii did not break the dormant state of the seed by gibberellin treatment without low temperature stratification treatment. In the gibberellin treatment, germination performance was improved according to the low temperature stratification treatment period. As a result of this study, the seeds of S. williamsii have both an intermediate complex and a deep complex morphophysiological dormancy (MPD). In comparison, it was found that the S. racemosa subsp. pendula had intermediate composite MPD.

Plant Protective and Growth Promoting Effects of Seed Endophytes in Soybean Plants

  • Jiwon Kim;Seong-Ho Ahn;Ji Sun Yang;Seonwoo Choi;Ho Won Jung;Junhyun Jeon
    • The Plant Pathology Journal
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    • v.39 no.5
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    • pp.513-521
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    • 2023
  • Seed-borne diseases reduce not only the seed germination and seedling growth but also seed quality, resulting in the significant yield loss in crop production. Plant seed harbors diverse microbes termed endophytes other than pathogens inside it. However, their roles and application to agricultures were rarely understood and explored to date. Recently, we had isolated from soybean seeds culturable endophytes exhibiting in-vitro antagonistic activities against common bacterial and fungal seed-borne pathogens. In this study, we evaluated effects of seed treatment with endophytes on plant growth and protection against the common seed-borne pathogens: four fungal pathogens (Cercospora sojina, C. kikuchii, Septoria glycines, Diaporthe eres) and two bacterial pathogens (Xanthomonas axonopodis pv. glycines, Pseudomonas syringae pv. tabaci). Our experiments showed that treatment of soybean seeds with seed endophytes clearly offer protection against seed-borne pathogens. We also found that some of the endophytes promote plant growth in addition to the disease suppression. Taken together, our results demonstrate agricultural potential of seed endophytes in crop protection.