• Korean Journal of Plant Resources
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• v.21 no.5
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• pp.347-351
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• 2008

Rhizomes of Acorus gramineus Soland have been used as sedatives, analgesics, and stomachics in Korean medicine. Even though A. gramineus produced in Korea is known as having better efficacy of a remedy than that in China, its cultivation area has not increased because of the lack of seedlings. To solve this problem, seed propagation method was tested. Seeds were harvested and sowed three times in June, 2005. The best results showed at greenish yellow seed stage harvested in mid June, and its germination ratio was 90.7%. Seeds were well stored at $5^{\circ}C$ for 30 days. As for in vitro culture, multiple shoots were induced first in MS basal medium supplemented with $2.0mg{\cdot}L^{-1}$ NAA plus $0.1mg{\cdot}L^{-1}$ BA, and then roots were induced in MS basal medium containing $0.1mg{\cdot}L^{-1}$ BA or $0.5mg{\cdot}L^{-1}$ NAA plus $1.0mg{\cdot}L^{-1}$ BA. Growth of A. gramineus seedlings in Wonyesangto, potting material sold in markets for horticultural plants, was superior to vermiculite.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.37 no.2
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• pp.117-122
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• 1992

This experiment was conducted to determine tuber yield increase of Konjac by sowing time, seed tuber split method and cultural methods. Tuber yield per unit area was generally increased in early planting than conventional planting time. Tuber yield of polyethylene film mulching culture among storage methods was high, while the emergence rate of konjac for seedling plus PE mulching, and tunnel culture were prompted by 20 days, and especially seedling was to be greatly controlled due to temperature difference of the day and night at emergence. All of the cutting methods(two and four split method) were higher than conventional method because of increasing number of bulblet, accordingly, that method using seed tuber was profitable for mass propagation. Both botanical characteristics and tuber yield tend to be increased at 30 to 50 percent shading than natural condition.

• Horticultural Science & Technology
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• v.34 no.3
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• pp.363-371
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• 2016

Tabulis [Astilboides tabularis (Hemsl.) Engl.] has the potential for becoming a useful horticultural crop. This study was conducted to classify the seed dormancy types and to identify the germination conditions that improve the horticultural usefulness of this plant. We found that A. tabularis (Hemsl.) Engl. produced undifferentiated embryos and had a low germination rate of under 50%. In addition, water submersion led to moisture absorption. Therefore, A. tabularis seeds were designated as morphologically dormant. The germination rate was highest (86.3%) at $30^{\circ}C$ under light conditions. Higher temperatures generally led to an acceleration of the germination process, regardless of light condition. To improve the germination rate, seeds were submerged in various concentrations of growth regulators, such as $GA_3$ and kinetin, as well as minerals such as $KNO_3$ and KCl. An analysis of germination characteristics at $30^{\circ}C$ under light conditions revealed that treatment with 200 and $500mg{\cdot}L^{-1}GA_3$ led to an excellent germination rate of 97.0%. Treatment with $100mg{\cdot}L^{-1}GA_3$ led to a better germination rate than the control as well. However, treatment with kinetin, $KNO_3$, and KCl had no effect on germination, regardless of concentration.

• Journal of Plant Biotechnology
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• v.6 no.3
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• pp.165-169
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• 2004

The fruits of Vitex rotundifolia in Korea, known as 'Man Hyung Ja', occupy an important position as traditional oriental medicine in Asian countries. It is known that propagation of this plant by seed is difficult and time-consuming with little success. Attempts were made to develop a method by using nodal culture techniques. Explants of stem node without leaves cultured on Nitsch medium containing 1 ml/L BA, gave the best shoot induction ratio. Also, BA with IAA or TDZ treatment showed positive effect on shoot induction. Half-strength Nitsch medium was supplemented with 0.5 mg/L NAA produced better success than did the others on root formation. It showed that many of the regenerants grew successfully on growth chamber at $24^{\circ}c$.

• Asian Journal of Turfgrass Science
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• v.9 no.1
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• pp.1-10
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• 1995

This study was carried out to investigate the method of propagation of Lindera erythrocarpa Makino. The results were as follows: 1. After the treatment of warm stratification for 1 month followed by cold stratification for 3 months, the germination ratio of seeds of Lindera erythrocarpa was from 84.6 to 93.6 percent. But, dry stored seeds lose their viability Quickly and should be placed in pretreatment im-mediately. Also, the seeds of control district germinated from 68.6 to 74.6 percent. So it was confirmed that the seed of Lindera erythocaeicarpa was not doubly dormant. 2. Softwood cuttings of Lindera erythrocarpa with 5,000 ppm IBA on August 4. 1992 resulted in the best rooting of 95.0 percentage. 3.Twenty percent of the graft union were obtained in the green wood grafting in Lindera erythrocarpa on June 7, 1993. And five percent of the graft union were obtained in the hard wood grafting in Lindera erythrcoar pa on April 6. 1993.

• Journal of the Korean Institute of Landscape Architecture
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• v.21 no.3
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• pp.99-104
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• 1993

The objectives of this study were investigation of the sutiable propagation method by softwood cuttings of female Ilex serrata, the comparison of sex determination of Ilex serrata after seeding and the fruiting percentage of the Ilex serrata male and female. The results obtained were as follows. 85 percentage of the seed were germinated with three months cold stratification. 35 percentage of female plant were observed after seeding. Cuttings should be rooted on late June using a quick dipping of 1,000ppm IBA solution. The rooting rate of the softwood cuttings treated IBA 1,000pm on June 22 in a closed moist chamber with 50% shade screen was 72.2 percentage. The suitable planting of male and female of Ilex serrata was male one versus female thirteen in a distance of 50 meter.

• Korean Journal of Plant Tissue Culture
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• v.24 no.4
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• pp.239-256
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• 1997

Plants belonging to the category of 2n apomixis or agamospermy form embryos and seeds without the processes of normal meiosis and syngamy. Seeds produced in this way have identical genotype of their maternal parent. Three different types of agamospermy are recognized: diplospory, apospory, and adventitious (adventive) embryony. $F_1$ hybrid cultivars cannot be used as seed sources in the next ($F_2$) generation because this generation would be extremely variable as a result of genetic segregation. Hybrid vigor is also reduced in the $F_2$ generation. Therefore, parental stocks for hybrid seed production need to be maintained and cross must be continuously repeated. Agamospermic 2n apomixis would make it possible to fix the genotype of a superior variety so that clonal seeds faithfully representing that genotype could be continuously and cheaply produced independent of pollination. That is, $F_1$ hybrid seeds could be produced for many generations without loss of vigor or genotype alteration. Production of apomictic $F_1$ hybrid seed would be simplified because line isolation would not be necessary to produce seed or to maintain parental lines, and the use of male-sterile lines could be avoided. Overall, apomixis would enable a significant reduction in hybrid seed production costs. Additionally, the production of clonal seed is not only important for seed propagated crops, but also for the propagation of heterozygous fruit trees and timbers. Clonal seed would help avoid costly and time-consuming vegetative propagating methods that are currently used to ensure the large-scale production of these plants. Apomixis is scattered throughout the plant kingdom, but few important agricultural crops possess this trait Therefore, most research to date has centered on introgressing the trait of apomixis into agricultural crops such as wheat, maize, and some forage grasses from wild distant relatives by traditional cross breeding. The classical breeding approach, however is slow and often impeded by many breeding barriers. These problems could be surmounted by taking mutagenesis or molecular approach. Arabidopsis thaliana is a tiny sexually reproducing plant and is convenient in constructing and screening in molecular researches. Male-sterile mutants of Arabidopsis are particularly suitable genetic background for mutagenesis and screening for apomictic mutants. Molecular approaches towards isolating the genes controlling the apomictic process are feasible. Direct isolation of genes conferring apomixis development would greatly facilitate the transfer of this trait to wide variety of crops. Such studies are now in progress.

• Journal of Bio-Environment Control
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• v.32 no.3
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• pp.210-216
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• 2023

Conventionally, the seeds of Rehmannia glutinosa (Gaertn.) Libosch. ex Steud. have been directly sown at the field without using the plug seedling method. Plug seedlings have the advantage of promoting germination and convenient transplanting. However, there is little information about propagation of R. glutinosa using the plug seedling method. This study was conducted to investigate the optimal seed rhizome length, diameter, and sowing direction of R. glutinosa for establishing the plug seedling method. Seed rhizome length and diameter were separated by 1, 2, 3 cm and 0.3-0.5, 0.6-1.0, 1.1-1.5 cm, respectively. And seed rhizomes were sown in vertical and horizontal directions. The survival rate in 1 cm length of seed rhizomes was lower than in other treatments. The leaf length, leaf width, number of leaves, SPAD, leaf area, and fresh and dry weights of shoot and roots were the greatest in 3 cm length of seed rhizomes. As the seed rhizome diameter decreased, the growth characteristics of R. glutinosa tended to increase. When R. glutinosa was sown horizontal direction, the leaf length, leaf width, number of leaves, and leaf area were significantly higher than in the vertical direction. In conclusion, when sowing seed rhizome in a plug tray, using a length of 3 cm, a diameter of less than 1 cm, and sowing in a horizontal direction is considered an appropriate sowing method for R. glutinosa.

• Journal of Plant Biotechnology
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• v.1 no.2
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• pp.97-100
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• 1999

In order to establish in vitro propagation method of sundew, Drosera rotundifolia L., the effects of MS medium concentration, cytokinin type and concentration, pH, and auxin type and concentration on shoot proliferation and root formation were investigated using shoots at 3 month after seed germination. The highest shoot production was obtained with the half strength of MS ($\frac{1}{2}$ MS) medium than with any other strength of MS medium tested. Addition of kinetin or BA in $\frac{1}{2}$ MS medium was strongly suppressed shoot proliferation. The suppression of shoot proliferation was more effective in BA-supplemented $\frac{1}{2}$ MS medium than kinetin-supplemented. The optimum pH of the media for shoot proliferation was pH 5.7-6.7. Shoots were subcultured in $\frac{1}{2}$ MS medium supplemented with 0.5mg/L 2,4-D for rooting every 8 weeks. All subcultured shoots produced extensive root systems after 5 to 6 week culture. Plantlets after root development were planted in plastic pots filled with moss. The survival rate of plantlets was almost 100%. On subculturing every 8 weeks, hundreds of the plants were propagated from a single plant within a year.

• 한국약용작물학회:학술대회논문집
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• 2007.05a
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• pp.89-90
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• 2007