• Korean Journal of Plant Resources
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• v.35 no.6
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• pp.796-804
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• 2022

The demand for Chelidonium majus L. subsp. asiaticum H. Hara is expected to increase due to its pharmacological properties such as antibacterial, antioxidant, and anti-inflammatory effects. However, an effective propagation system for this species has not yet been established. This study was conducted to analyze the seed dormancy and germination characteristics of C. majus L. subsp. asiaticum H. Hara native to Korea and establish a mass propagation system. The dormancy type was primarily classified by analyzing the general information of the collected seeds. The seed dormancy breaking was investigated by comparing the effects of cold stratification (0, 2, 4, 8, 10, or 12 weeks) with warm stratification (S, summer temperature, 25/15℃) and intermediate temperature stratification (A, autumn temperature, 15/10℃) of alternating temperature stratification (S12-A4 or S12-A8 weeks). After dormancy break, 500 mg/L GA₃ and GA₄₊₇ treatment replaced cold stratification and improved seed germination. The results of this study are expected to provide basic data for future seed propagation and mass propagation by analyzing the dormancy and germination characteristics of C. majus L. subsp. asiaticum H. Hara seeds.

• Asian Journal of Turfgrass Science
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• v.18 no.1
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• pp.29-36
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• 2004

The efficient refinement of seed is required to reduce the cost and labor input in artificial propagation of wild plant. This study was carried out to develop methods for collecting and refining tiny seeds from wild plants. For obtaining Phragmites communis seeds, the inflorescence was cut into small fragments using a Straw Cutter and subsequently detached pappus hairs from seed coat by Hammer Mill. The primary refined seeds were passed 1.0 mm sieve. The screened seeds were subjected to Seed Blower with wind speed of 0.25 mㆍsec-1 to collected intact and well-ripen seeds. The seeds of Imperata cylindrica were refined as follows. Inflorescences were cut using a Straw Cutter first. The pappus was removed from cut fragments using a Hammer Mill and subsequently subjected to Seed Scarifier at 500rpm for 60 sec. for further separation. The separated seeds were passed 1.0 mm screen and collected after blowing with Seed Blower of wind speed of 0.15 mㆍsec-1. When the amount of seed was too little to refine with Seed Scarifier and Blower, the procedure was slightly modified from the procedure described above. The crude seed mixture obtained from Hammer Mill step was hand-refined roughly and then immersed into cone. (95%) sulfuric acid for 2 min. and collected floating portion after dilution of sulfuric acid solution 100 times with tap water. The collected seeds were dried and passed 0.149 mm sieve. During seed refining process using mechanical or sulfuric acid treatments, a small portion of damaged seed were evolved, however, the amount was not noticeable as compared to the total amount of collected seeds. Because the germination percentages between hand-refined seeds and seeds refined by above methods were not statistically different, the developed procedures for refining tiny seed of wild plants are helpful to reduce the cost and labor input in artificial propagation of two species.

• Plant Biotechnology Reports
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• v.3 no.1
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• pp.1-56
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• 2009

Orchid seeds are nearly microscopic in size. Because of that, many fanciful theories were proposed for the origin of orchids. Almost 400 years separate the time when orchid seeds were seen for the first time and the development of a practical asymbiotic method for their germination. The seeds were first observed and drawn during the sixteenth century. Seedlings were first described and illustrated in 1804. The association between orchid and fungi was observed as early as 1824, while the requirement for mycorrhiza for seed germination was established in 1899. An asymbiotic method for orchid seed germination was developed in 1921. After Knudson's media B and C were formulated, orchids growing and hybridization became widespread. Hybrids which early growers may not have even imagined became possible.

• Korean Journal of Plant Resources
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• v.22 no.6
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• pp.518-521
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• 2009

High productivity of ginger (Zingiber officinale Roscoe) was obtained from the rhizome produced by shoot-tip culture with Korean native variety, Seosanjong. Seed rhizomes induced by shoot-tip culture were successfully established in the field. The rhizomes induced by both plant or rhizome were higher in emergence rate and faster in days to emergence than those of home seed production. The seed rhizome production induced by shoot-tip culture was two times heavier than that of home seed production. These results suggest that shoot-tip culture might be one of mass propagation methods in seed rhizome of ginger plant.

• Korean Journal of Plant Resources
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• v.14 no.1
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• pp.53-59
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• 2001

This study was carried out to investigate mass propagation technique by using seed and vegetative propagation of Kalopanax pictus Nakai. For developing seed propagation, seed stratification and 1$0^{\circ}C$ after-ripening treatment induced embryo growth within 1 weeks, resulted in increasing germination rate of seeds up to more than 65% when planted. The softwood cutting using one year old shoot increased rooting rate to 69% whereas more than 1 year old shoot looked like inappropriate for cutting propagation. In the cutting timing, the rooting rate on June, 13 cutting of the first growth shoot was the highest, followed by June 20 and July 4. The most efficient cutting timing seemed to be the middle of June. When cutted shoots were soaked for 30 minute with IBA and NAA 1000mg.$L^{-1}$, rooting rate was increased above 70%. As the concentrations of plant hormone were increased above 2000mg.$L^{-1}$, the rooting rate was slowly decreased.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.11 no.3
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• pp.12-19
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• 2008

This study was conducted to investigate the draft data for the habitat environment and massive propagation method of Miscanthus changii, which is classified as rare species. The in-site analysis of the native soil give a indication of pH 5.24~5.48, electric conductivity (EC)0.03~0.02 $dS{\cdot}m^{-1}$, organic matter (OM) 1.13~1.15%, Ca 3.4 $mg{\cdot}kg^{-1}$, K 0.25 $mg{\cdot}kg^{-1}$, Mg 0.53 $mg{\cdot}kg^{-1}$, N 0.23%, P 2.15 $mg{\cdot}kg^{-1}$ showed low and 0.33~9.12 $cmol{\cdot}kg^{-1}$ CEC. Growth and tiller number in rhizome propagation of Miscanthus changii showed that horticulture media (perlite 1+vermiculate 1+peat moss 1) is the most suitable soil to habitat. As for seed propagation, germination percentage is 0% (0/20) at first seeding, 2% (1/50) at second seeding and 2.1% (6/280) at third seeding. The average germination percentage was below 2%. This is the reason why Miscanthus changi disappear at habitat of poor soil environment and low germination. We gained 61 plants that 60 rhizome propagation and 1 by seed propagation, renatural to habitat.

• Journal of applied mathematics & informatics
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• v.9 no.1
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• pp.303-310
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• 2002

The cyryptographic strength of a Feistel cipher depends strongly on the properties of its S-boxes, which are the strict avalanche criterion(SAC), the propagation criterion(PC) and GAC(the global avalanche criterion). In this paper global avalanche characteristics of S-boxes of the SEED are in-vestigated and compared to global avalanche characteristics of S-boxes of S-boxes of the Data Encryption Standard(DES).

• Proceedings of the Korean Society of Plant Biotechnology Conference
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• 2002.04a
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• pp.33-40
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• 2002

We, Tong Yang Moolsan Co. Ltd. (TYM) set up the mass-production system for virus-free seed garlic via tissue culture technique. TYM's tissue culture technique is called as 'Multiple shoot propagation technique'. This technique can lead mass propagation of genetically homogeneous seed garlic in a short period because of its highly proliferation rate of in vitro shoots ($15^{10}$ /year). TYM researchers applied the technique to some selected garlic cultivars with superior characteristics and carried out field test of productivity in the inside and outside of the country for several years. According to the yearly results of field test with virus-free seed garlic, we ascertained that virus-free seed garlic can produce the highly yield increase (max. above $50\%$) and also can enhance the product quality. Consequently, we estimated that TYM's seed garlic will contribute to farmers with increase of income and can elevate the national position of garlic market in the world for its competitive power of technical and production cost.

• Korean Journal of Medicinal Crop Science
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• v.9 no.1
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• pp.55-61
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• 2001

In this study, effects of wiling peduncle on production yield and crude saponin content in Anemarrhena asphodeloides Bunge were investigation. The peduncle cutting were conduct on May 30, June 15, June 30, non-cutting of seed propagation type and young plant propagation type in Anemarrhena asphodeloides Bunge. The highest rhizome yield was 276.9g/plant on May 30, cutting puduncle in seed propagation type. The rhizome yield at seed propagation type was increased about 40.8%, compared to that at non-cutting. Crude saponin content in rhizome was 7.50% on May 30, cutting puduncle in seed propagation type. The crude saponin content at seed propagation type was increased about 32.2%, compared to that at non-cutting.

• Journal of the Korean Institute of Landscape Architecture
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• v.25 no.2
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• pp.9-19
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• 1997

These studies were carried out to investigate seed propagation method and conducted using stem cuttings of Styrax japonicus for using as a woody landscape plant. The results are obtained as follows. After the H\sub2\SO\sub 4\ and GA\sub 3\ treatment to the seeds of Styrax japonicus, three months of warm treatment followed by cold stratification for four months increased seed germination rate. The rooting rate of Styrax japonicus was increased by the treatment with concentrated IBA, especially highest at 7,000ppm. Rooting percentages were highest for cuttings taken on July 15, 1995. Therefore, the most effective method for rooting of Styrax japonicus was treatment with 7,000ppm IBA on July 15 cuttings, which showed rooting rate of over 90%. Half-leaf-remained cuttings were more effective than those with two leaves on rooting of Styrax japonicus. Dipping for 10 seconds in IBA solution resulted in rooting rate of over 70%. The longer the treatment time the worse the rooting.