• Title/Summary/Keyword: Seed germination

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Studies on the Germination Promotion of the Zoysia japonica seeds. (한국잔디(Zoysia japonica Steud.) 종자의 발아촉진에 관한 연구)

  • 전우방
    • Asian Journal of Turfgrass Science
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    • v.1 no.1
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    • pp.30-36
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    • 1987
  • In order to promote the germination of Korean lawn grass(Zoysia japonica Steud.) seeds a gravimetric seed selection with a mixed chemical solution and the germination promotion with KOH treatment were studied and following results were obtained. 1. When immersed into methanol(S.G., 0.7) 14 percent of immature seeds were precipitated. 2. With water (S.G., 1.0) 36.5 percent of mature seeds were submerged. 3. On a mixture of carbon tetrachloride (S.G., 1.6) and ethylether (S.G., 0.7) in a ratio of 4:6 the seed selection was most effective. 4. When treated with KOH solution the germination rate was 71.01 percent in contrast to 6.0 percent of germination without the treatment. 5. As for concentration of KQH solution, 15 percent depressed the germination (46.7 %) whereas above 20 % the germination rate was gradually increased (79.32 %) up to 40 percent of concentration where the concentration started to depress germination ratio (77.32 %). 6. Finally, in connection with the duration of KOH treatment, a time lapse of up to 20 minutes depressed the germinating ratio(70.02 %) and 30 minutes to 45 minutes increased this ratio to 94.52 percent. 60 minutes of treatment depressed the germination to 75.62 percent.

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Effects of Provenances, Storage Temperature and Duration on Seed Germination of Bombax costatum Pellegr & Vuillet

  • Omoyemi, Ojo Morenike;Olatunji, Asinwa Israel
    • Journal of Forest and Environmental Science
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    • v.37 no.3
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    • pp.235-242
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    • 2021
  • Seed morphology, physiology and environmental conditions have influence on germination of any tropical seeds and their appropriate handling, processing and handling enhances seeds emergent. This study therefore investigated effects of storage durations under different temperatures on germination of Bombax costatum seeds from different provenances. Fresh 25 seeds of B. costatum in four replicates were collected from four different provenances (Aponmu, Oluwa, Ibadan and Oyo). They were surface sterilized, thoroughly washed in distilled water and stored at four different temperature regimes: 28℃, 21℃, 5℃ and -17℃. Samples were taken every 2 months for germination test for 18 months. Germination assessment was carried out daily and recorded. The experimental design was 4×4×10 factorial in 4 replicates. Data were subjected to percentages and analysis of variance (ANOVA). Results showed that there were significant differences among storage temperatures, storage durations and provenances on germination percentage. Freshly collected seeds from Aponmu and Oyo had the highest mean germination of 100% followed by seeds from Ibadan (89.3%). Seeds from Oyo provenance stored 5℃ had germination of 94.0%, 88.70% and 78.7% at 2nd, 4th and 6th month respectively. Seeds from Ibadan and Oyo stored at 28℃, 21℃ recorded 0.0% starting from 8 months of storage. Germination of B. costatum seeds from the four provenances decreased with increase in storage duration at different temperatures.

Germination Percentage and Speed under International Seed Testing Association Conditions of Main Herbaceous Plants Used on the Slope (국제종자검정협회(ISTA) 변온조건에서 비탈면 복원용 주요 초화류의 발아율 및 발아속도)

  • Park, Jin-A;Shim, Sang-Ryul
    • Journal of the Korean Society of Environmental Restoration Technology
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    • v.21 no.3
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    • pp.47-56
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    • 2018
  • Research was initiated to investigate germination characteristics and germination pattern of 14 herbaceous plant entries used on the road slope during 30 days. An alternative germination condition for 14 herbaceous plant entries required by International Seed Testing Association(ISTA) was applied in the experiment, consisting of 8-hr light at $25^{\circ}C$ and 16-hr dark at $15^{\circ}C$. Significant differences were observed in the first germination rate(0.3%~40.7%)and in the final germination rate(7.7%~93.3%). Days to the first germination(2~8days), days to the 30% germination(2~6days) and days to the peak germination(6~18days) were different among 14 herbaceous plant entries in the study. From this result, we could find out higher final germination rate of 14 herbaceous plant entries in the following order; First, forage crops and cool-season turfgrasses; Second, herbeceous flowers; Third, wild plants. We could also divide germination rate among 14 herbaceous plant entries as 6 groups(I;very high, II;high, III;medium high, IV;medium low, V;low, VI;very low) based on the final germination rate and divide germination speed as 5 groups(A;very fast, B;fast, C;normal, D;slow, E;very slow)based on days to the peak germination. Considering germination characteristics and pattern of 14 herbaceous plant entries Medicago sativa, Lolium perenne, Festuca arundinacea and Cosmos sulphureus were regard as dominating species while Lespedeza cuneata, Silene armeria, Lotus corniculatus var.japonicus, Coreopsis tinctoria and Centaurea cyanus as competitive species following dominating species. However, Chrysanthemum burbankii, Pennisetum alopecuroides, Chrysanthemum boreale., Artemisia princeps var. orientalis and Arundinella hirta were not almost expected to emerge.

Allelopathic Effect against Seed Germination and Seedling Growth of Picea jezoensis of Leaf Aqueous Extracts from Competition Species (경쟁 수종들의 잎 추출 수용액이 가문비나무 종자발아 및 유묘생장에 미치는 타감효과)

  • Kim, Gil Nam;Han, Sim-Hee;Shin, Soo Jeong;Kim, Du-Hyun
    • Journal of Korean Society of Forest Science
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    • v.105 no.1
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    • pp.86-92
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    • 2016
  • This study was conducted to evaluate the allelopathic effect against the regeneration of the seedling and to identify the presence of allelochemicals in Picea jezoensis natural population in Jirisan. Water-soluble extracts from leaves of different competition plants were collected to test their effects on seed germination and seedling growth of P. jezoensis. Phenolic compounds from leaves were quantified using GC/MS. The seed germination rate and seedling growth of P. jezoensis was reduced by extracts of all competition plants leaves. Monoterpenoids compound, which are generally well known in the allelochemicals has been detected in the leaf extracts. In conclusion, allelopathic chemicals of competition vegetation in P. jezoensis natural population could inhibit the seed germination and seedling growth of P. jezoensis, that is considered as a result of the lower seedling establishment.

Effect of Aging on the Chemical Forms and Phytotoxicity of Arsenic in Soil (비소 오염기간이 토양 내 비소의 존재형태와 식물독성에 미치는 영향)

  • Yang, Woojin;Jho, Eun Hea;Im, Jinwoo;Jeong, Seulki;Nam, Kyoungphile
    • Journal of Soil and Groundwater Environment
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    • v.21 no.3
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    • pp.82-87
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    • 2016
  • This study investigates effects of an aging period on arsenic (As) chemical forms in soils and phytotoxicity using artificially As-contaminated soils with a range of As concentrations (0-300 mg/kg) and aging periods (0 and 3 months). A sequential extraction procedure showed that the increasing As concentration in soils increased the ratio of non-specifically and specifically bound As, which are known to be bioavailable. This resulted in increasing As uptake by tomatoes with increasing As concentration (R2=0.87 for exponential fitting); however, the seed germination was not sensitive to the As concentrations of the soil samples. The seed germination was also statistically similar in the soils with 75 and 150 mg-As/kg regardless of the aging period. The time taken until the seed germination (i.e., lag phase), on the other hand, decreased from 10 d to 3 d with aging for 3 months. This can be attributed to the decreased amount of bioavailable As with aging. Overall, this study shows that when the toxic effects of the As-contaminated soils are assessed using tomato plants, it is better to use more sensitive methods than seed germination such as the As accumulation or the lag phase for seed germination.

Allelopathic Effect of Ganghwa mugwort (Artemisia spp.) on Seed Germination and Seedling Growth of Plants (강화약쑥 추출물이 종자발아 및 유식물 생장에 미치는 알레로파시 효과)

  • Lee, Joo-Hwa;Byeon, Ji-Hui;Lee, Jeong-Hoon;Park, Chun-Geon;Park, Chung-Berm;Cho, Joon-Hyeong
    • Korean Journal of Organic Agriculture
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    • v.20 no.4
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    • pp.589-605
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    • 2012
  • This study was conducted to identify allelopathic effect of Ganghwa domestic Artemisia spp., named Sajabalssuk and Ssajuarissuk, for various receptor plants including clover (Trifolium repens L.), alfalfa (Medicago sativa L.), lawn grass (Zoysia japonica Steud.), dandelion (Taraxacum platycarpum Dahlst.), and dahurianpatrinia (Patrinia scabiosaefolia Fisch. ex Trevir). Receptor plants were treated with the aqueous and essential oil extract of Artemisia plants. In consequence, their allelopathic effects were evaluated by measuring seed germination rates, seedling growth, and dry weights of the receptor plants. The seed germination and seedling growth of the receptor plants were inhibited by all treatments of both aqueous and essential oil extracts of the Artemisia plants, and, in addition, the inhibitory effects were increased according to the higher concentration. Among the donor plants, A. $sp.^*III$ showed most effective allelopathic effect. Comparing the alleopathic effect among the receptor plants, seed germination was most inhibited in lawn grass while inhibitory effect of seedling growth was comparatively higher in dandelion. Although inhibitory effects were comparatively lower, the allelopathic effects of Artemisia plants were identified in clover and alfalfa since the seedling growth of these plants were inhibited more than 70%. Thus, in result, Ganghwa domestic Artemisia spp. could be possibly used for weed control since natural products of the plants showed inhibitory effects on seed germination and seedling growth of various receptor plants.

Effect of Seed Weight and Storage Method on Germination and Seedling Growth of Camellia japonica (동백나무의 발아 및 유묘 생장에 미치는 종자 무게 및 저장방법의 영향)

  • Kang, Hee-Kyoung;Choi, Su-Ji;Song, Hong-Seon
    • Korean Journal of Plant Resources
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    • v.33 no.1
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    • pp.33-39
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    • 2020
  • This study was carried out to provide basic information for mass breeding and cultivating the saplings of Camellia japonica. The germination rates did not show definite tendency according to the changes of seed weight at room temperature and 4℃ dry storage conditions. The germination rate of 4℃ wet sand storage was increased with seed weight, but showed very low germination rate for 120 days of storage. The germination rate was above 80% in 60 days, 90 days and 120 days, respectively, at 4℃ wet filter paper storage conditions. The average days taken for those germinations were about 30 days at room temperature and 4℃ dry storage conditions, but wet storage (sand, filter paper) condition took the shortly nearly 13 days for those germinations. Leaf number, leaf dry weight, stem diameter and root dry weight were higher than total average by more than 1.21 g for seed weight. Stem length was higher than the average by more than 1.01 g, plant dry weight was higher than the average by more than 0.81 g, and stem dry weight was higher than the average by more than 0.61 g. In the case of seed weight becoming heavier, seedling growth was good but T/R ratio tended to decrease. In order to increase the mass production of seedlings using Camellia japonica seeds, we need to specify the weight and size of those seeds. The 4℃ wet filter paper storage condition was evaluated as the most efficient method for the seed storage used for seeding.

Production of a Phytotoxic Compound, 3-Phenylpropionic Acid by a Bacterial Endophyte, Arthrobacter humicola YC6002 Isolated from the Root of Zoysia japonica

  • Chung, Eu-Jin;Park, Joo-Hwang;Park, Tae-Soon;Ahn, Jong-Woong;Chung, Young-Ryun
    • The Plant Pathology Journal
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    • v.26 no.3
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    • pp.245-252
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    • 2010
  • An endophytic bacterial strain, Arthrobacter humicola YC6002, was isolated from a surface sterilized root of Korean turf grass (Zoysia japonica) collected from Jinju, Korea. This strain showed inhibitory effect on germination and shoot growth of radish. The inhibition of germination and shoot growth of radish seeds varied depending on the age of culture and the temperature at which it was incubated. The culture filtrate of 1/10-strength Tryptic Soy Broth medium, incubated for 48 hours at $30^{\circ}C$, showed the highest inhibitory effect on radish seed germination and shoot growth (92% inhibition as compared to control). The active compound with seed germination and shoot growth inhibition was purified and identified as 3-phenylpropionic acid. The purified compound had 53% and 93% inhibitory effect on seed germination and shoot growth of radish for 500 and 1000 ppm solutions, respectively.

Molecular Identification and Fine Mapping of a Major Quantitative Trait Locus, OsGPq3 for Seed Low-Temperature Germinability in Rice

  • Nari Kim;Rahmatullah Jan;Jae-Ryoung Park;Saleem Asif;Kyung-Min Kim
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2022.10a
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    • pp.283-283
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    • 2022
  • Abiotic stresses such as high/low temperature, drought, salinity, and submergence directly or indirectly influence the physiological status and molecular mechanisms of rice which badly affect yield. Especially, the low temperature causes harmful influences in the overall process of rice growth such as uneven germination and the establishment of seedlings, which has become one of the main limiting factors affecting rice production in the world. It is of great significance to find the candidate genes controlling low-temperature tolerance during seed germination and study their functions for breeding new rice cultivars with immense low-temperature tolerance during seed germination. In this study, 120 lines of Cheongcheong/Nagdong double haploid population were used for quantitative trait locus analysis of low-temperature germinability. The results showed significant difference in germination under low different temperature conditions. In total, 4 QTLs were detected on chromosome 3, 6, and 8. A total of 41 genes were identified from all the 4 QTLs, among them, 25 genes were selected by gene function annotation and further screened through quantitative real time polymerase chain reaction. Based on gene function annotation and level of expression under low-temperature, our study suggested OsGPq3 gene as a candidate gene controlling viviparous germination, ABA and GA signaling under low-temperature. This study will provide a theoretical basis for marker-assisted breeding.

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Effect of Capsule Position on the Seed Germination and Juvenile Growth of Sesame (Sesamum indicum L.) (삭과의 착생부위(着生部位)가 참깨종자(種子)의 발아(發芽) 및 초기생육(初期生育)에 미치는 영향(影響))

  • Kim, Choong Soo
    • Korean Journal of Agricultural Science
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    • v.9 no.1
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    • pp.223-230
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    • 1982
  • To obtain the information on the germination and initial growth of the sesame, the seed of 6 sesame cultivars obtained from upper and lower position of plants were seeded and the results on the germination and initial growth were summarized as follows; 1. When the seeding time was delayed, the days to flower of all sesame cultivars were significantly decreased. And the shortening rate of days to flower by delaying seeding time showed significant differences among the sesame cultivars. 2. At all cultivars excluding IS 103, the germination percentage of the seeds obtained from lower position of sesame plant was significantly higher than those from upper position. 3. Germination percentage of IS 103 seeds from lower position of sesame plant was 20.0% at petri dish experiment and 75.0% at pot experiment and those from upper position of the stem was 17.0% at petri dish experiment and 72.5% at pot experiment, respectively. 4. The germination speed of all cultivars showed a same tendency as germination percentage, but the germination speed of cultivars at pot experiment was higher than those at petri dish experiment. 5. The sesame seedlings from lower position seed were increased in stem length, root length, leaf length and fresh weight than those from upper position seed. The germination percentage and the initial growth showed significant differences by the seed weight in same cultivar, however did not show any varietal differences.

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