• 제목/요약/키워드: Seed germination

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Presoaking with $GA_3$ Improves Germination of Zoysiagrass (Zoysia japonica Steud.) Seed on Poor Germination Conditions (발아불량 환경조건에서의 $GA_3$ 침지처리에 의한 들잔디의 종자발아촉진)

  • 구자형;윤병한
    • Asian Journal of Turfgrass Science
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    • v.13 no.1
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    • pp.21-28
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    • 1999
  • The seeds of Zoysiagrass (Zoysia japonica Steud.) were soaked with GA3 50 ppm and primed with CaCl2 at -1.0 MPa for 4days at 23$^{\circ}C$ to identify presoaking and priming regimes that may improve germination in saline condition and with PEG 8000 at high temperature. Presoaked, primed, and untreated seeds were then germinated at 30 and $35^{\circ}C$. NaCl salinity stress consistently decreased the rate of germination of zoysiagrass seed. GA3 or CaCl2 alleviated the inhibitory effect of salinity on germination. However, total percent germination (G) and T50 of untreated control seeds significantly decreased and prolonged at $30^{\circ}C$ and $35^{\circ}C$ as NaCl salinity stress increased. Presoaked seeds with GA3 50 ppm for 4 days at $23^{\circ}C$ had significantly higher germination and lower T50 than untreated or primed with CaCl2 at -1.0 MPa for 4days at $23^{\circ}C$m and overcame the inhibitory effect of germination derived from PEG 8000. In addition, presoaked seed had higher, faster, and more uniform germination than untreated seeds after sowing in growing media in greenhouse.

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Effect of anaerobic digestate on seed germination of perennial ryegrass seeds

  • Byun, Ji Eun;Choi, Min Su;Seo, Un Kab;Ryoo, Jong Won
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2017.06a
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    • pp.211-211
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    • 2017
  • This experiments were conducted to investigate seed germination to evaluate the influence of anaerobic digestate on seed germination of perennial ryegrass seeds. The study conducted a germination experiment in petri-dishes, using perennial ryegrass seeds. The treatments were compared: non-treated control treated with distilled water, different concentration of anaerobic digestate. The germination percentage of perennial ryegrass seeds was highest in the fermented anaerobic digestate treatment. Root length of perennial ryegrass seeds was long by 4~5cm in the fermented anaerobic digestate with unfermented anaerobic digestate. In the relative root length ratio was by 30% higher in the in the fermented anaerobic digestate with unfermented anaerobic digestate. The germination index of perennial ryegrass seeds was high by 113% in the fermented anaerobic digestate compared to no treatment. The fermented anaerobic digestate have shown stimulatory effects in germination and development of root. Use of fermented anaerobic digestate can be recommended to farmers as a ecofriendly practice for better germination and growth.

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Effect of Temperature and Sulfuric acid treatment on the Germination of Hovenia dulcis Thunb (온도 및 황산처리가 헛개나무 종자의 발아에 미치는 영향)

  • Lee, Kang-Soo
    • Korean Journal of Medicinal Crop Science
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    • v.9 no.2
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    • pp.166-172
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    • 2001
  • This study was conducted to investigate the optimum condition of breaking dormancy and to increase the germination rate of Hovenia dulcis Thunb. seeds. Germination of seeds which did not germinate at the various constant temperature was induced by the artificial breaking of seed coat, and the germination rate of seeds which the seed coats were broken was the highest(94%) at $15^{\circ}C$. The germination rate of seed was highest when seeds were treated with concentrated $H_2SO_4$ for 40 minutes. When seeds were stored in dry condition, the germination capability was maintained up to 5 years after harvest. However, their germination rate become lowered and average germinating duration was extended. Germination rate of seeds which were stored for 5 years significantly increased by the low temperature treatment more than 30 days.

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Change in Germination and Physiological Properties of Hippophae rhamnoides Seeds by Different Storage Period (갈매보리수나무 종자의 저장기간에 따른 발아 및 생리적 특성)

  • Choi, Chung Ho;Yang, Byeong Hoon
    • Korean Journal of Plant Resources
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    • v.28 no.4
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    • pp.533-540
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    • 2015
  • Germination properties, leachate electrical conductivity (EC), and inorganic compound leaching were analyzed to ascertain the storage ability and change of physiological characteristics during storage of Hippophae rhamnoides seeds. Seeds were placed in an incubator at 25℃ and sown in different soil media (sand, vermiculite and horticultural substrate) after being stored for 6, 18 and 30 months at 2℃. All germination properties decreased in accordance to an increase of the seed storage period. Compared with the seed storage for 18 months, germination percentage (GP), germination performance index (GPI), and germination value (GV) of seeds stored for 30 months decreased by more than 50%. When the seeds were sown in different soil media in a greenhouse, those germination properties were similar to the seeds germinated in an incubator, and mean germination time, GPI and GV had a significant difference except GP among soil media. EC and inorganic ion concentration had a strong positive correlation with the seed storage period, but the ratios of inorganic ions from stored seeds revealed that K+/Mg2+ and Na+/Mg2+ were inversely correlated with the storage period.

The Effect of Seed-borne Mycoflora from Sorghum and Foxtail Millet Seeds on Germination and Disease Transmission

  • Yago, Jonar I.;Roh, Jae-Hwan;Bae, Soon-Do;Yoon, Young-Nam;Kim, Hyun-Ju;Nam, Min-Hee
    • Mycobiology
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    • v.39 no.3
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    • pp.206-218
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    • 2011
  • The seed-borne mycoflora of sorghum and foxtail millet collected from different growing areas in South Korea were isolated and taxonomically identified using dry inspection, standard blotter and the agar plate method. We investigated the in vitro and in vitro germination rates of disinfected and non-disinfected seeds of sorghum and foxtail millet using sterilized and unsterilized soil. The percent recovery of seed-borne mycoflora from the seed components of sorghum and foxtail millet seeds was determined and an infection experiment using the dominant species was evaluated for seedling emergence and mortality. A higher number of seed-borne fungi was observed in sorghum compared to that of foxtail millet. Eighteen fungal genera with 34 fungal species were identified from the seeds of sorghum and 13 genera with 22 species were identified from the seeds of foxtail millet. Five dominant species such as Alternaria alternata, Aspergillus flavus, Curvularia lunata, Fusarium moniliforme and Phoma sp. were recorded as seed-borne mycoflora in sorghum and 4 dominant species (Alternaria alternata, Aspergillus flavus, Curvularia lunata, Fusarium moniliforme) were observed in foxtail millet. The in vitro and in vitro germination rates were higher using disinfected seeds and sterilized soil. More seed-borne fungi were recovered from the pericarp compared to the endosperm and seed embryo. The percent recovery of seed-borne fungi ranged from 2.22% to 60.0%, and Alternaria alternata, Curvularia lunata and 4 species of Fusarium were isolated from the endosperm and embryo of sorghum and foxtail millet. Inoculation of the dominant seed-borne fungi showed considerable mortality of seedlings. All the transmitted seed-borne fungi might well be a primary source of infection of sorghum and foxtail millet crops.

Studies on the Relationship of the Seed Germination Testing Methods to the Field Emergence. (종자의 발아시험방법과 포장출현과의 관계)

  • 전우방
    • Asian Journal of Turfgrass Science
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    • v.3 no.1
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    • pp.13-15
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    • 1989
  • In oder to find out the effective seed germination testing method to the field emergence, an experiment was conducted ; 1. TTC testing results were higher percentage than any other germination testing methods . 2. On the corn seed , field emergence was highly correlated with germinator test, TTC test and AA test hut cold test was lower percentage . 3. Field emergence , on the soybean seeds was highly correlated with AA test and cold test but germinator test and TTC test was higher percentage .

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The Effect of Different Periods of Seed Storage on Germination of Genus Lespedeza (Lespedeza속(屬)의 종자저장기간별(種子貯藏期間別) 발아시험(發芽試驗))

  • Han, Young Chang
    • Journal of Korean Society of Forest Science
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    • v.31 no.1
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    • pp.30-36
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    • 1976
  • In order to guide the project for tree seedling production and direct seeding afforestation by introduction of genus Lespedeza, studies were conducted on the effect of seed storage period and on percentage and rate of germination through various kinds of treatments. L. bicolor, L. cyrtobotrya, and L. japonica. intermedia seed were storeds at different period of time. The results obtained are summarized as follows: 1. Germination capacity was not reduced until 28 months after harvest. However, germination was very poor 40 months after harvest, with less than 30% germination obtained by germination treatments. 2. Removal of seed coat and cone, sulphuric acid treatments were more effective in increasing germination than other treatments. Especially, most seeds germinated one week earlier than other treatments by removal of the seed coat. 3. The seeds stored for a short period of time showed more rapid germination compared to seeds which were stored for long period.

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Seed Characteristics and Accelerating Method of Germination in Bupleurum falcatum (시호 종자의 특성 및 발아촉진 방법 연구)

  • Eunil, Lee;Seok Hyeon, Kim
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.41 no.3
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    • pp.384-394
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    • 1996
  • The experiment was conducted to determine the seed characteristics and preferable methods to enhance the seed germination rate in Bupleurum falcatum. The optimum temperature for the seed germination of Bupleurum falcatum is 2$0^{\circ}C$. Any significant promoting effects were not found in seed germination with hormone treatments and physical methods. At 15$^{\circ}C$, prechilling combined with 50~200ppm of GA$_3$ treatment raised germination rate by 2 times of control ones. The most positive effect was observed in the treatment of 10$^{-2}$ ~10$^{-3}$ M potassium nitrate only at 15$^{\circ}C$ for 12 and 24 hours. The leachate of Bupleurum falcatum didn't inhibit the germination of Lactuca sativa, showing almost 100% of germination rate, which is suggested that no inhibitors contained in the seeds of Bupleurum falcatum. Observation of embryo conditions under stereoscopic microscope showed that the ratio of seeds with or without embryo is almost 50/50. The results suggested that the lower rate of germination in Bupleurum falcatum was caused by embryolessness of seeds.

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Effect of Presowing Seed Treatments on Germination and Seedling Emergence in Taraxacum platycarpum (민들레 종자의 파종전 처리가 발아율 및 입모율에 미치는 영향)

  • Jeon, Seung-Ho;Son, Daniel;Ryu, Yeong-Seop;Kim, Seok-Hyeon;Chung, Jong-Il;Kim, Min-Chul;Shim, Sang-In
    • Korean Journal of Medicinal Crop Science
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    • v.18 no.1
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    • pp.9-14
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    • 2010
  • Several presowing seed treatments have been used to facilitate the seed germination and seedling emergence. The study was performed to design a model of presowing seed treatments in Taraxacum platycarpum. Germination and seedling growth responses to temperature, drying, chilling, $GA_3$ and priming were investigated to know the optimum germination conditions and presowing treatment. The optimum temperature condition for germination and seedling growth were $15{\sim}20^{\circ}C$ at dark condition. The seeds exposed to infra red light, however, did not germinate. The moisture content of seeds dried at $35^{\circ}C$ for 3.5 hours was returned to the level of unimbibed seeds. The germination of seeds dried under dark condition was better than those dried under infra red light. Prechilling treatment imposed for 6 weeks showed higher germination rate than $GA_3$ treatment or priming. In field test, the highest field emergence was observed in the seeds dried after prechilled for 6 weeks and followed by drying followed by $GA_3$ > low temperature > $KNO_3$ priming. Imbibition with 0.1 mM $GA_3$ solution for 2 hours then drying at 35 for 3.5 hour under dark condition was effective to the practical presowing treatment for Taraxacum seeds.

Vitality and germination of Bletilla striata Rchb.f. seed according to treatment time and concentration of NaOCl (NaOCl 처리 농도 및 시간에 따른 희귀식물 자란(Bletilla striata Rchb.f.)의 종자 발아 및 활력 연구)

  • Jung, Young Ho;Kim, Jinki;Lee, Hayan;Song, Se-kyu;Bae, Kee Hwa
    • Journal of Plant Biotechnology
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    • v.47 no.1
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    • pp.73-77
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    • 2020
  • This study was conducted to examine the vitality of Bletilla striata Rchb.f. seed treated with different concentrations sodium hypochlorite (NaOCl) for different lengths of time. This study also examined the effect of NaOCl treatment times and concentrations on swelling formation and seed germination of B. striata seed. The non-treated B. striata seed had the highest survival rate (82.7%) Treatment with more than 1.5% NaOCl negatively affected the seed survival rate, as compared to concentrations of less than 1%. The swelling formation and seed germination percentages were highest (90.1% and 94.1%, respectively) when seeds were treated with 0.5% NaOCl for 20 min. These results can be used as important basic data for the growth and restoration of B. striata and further suggest the possibility of individual restoration in habitats.