• Journal of Microbiology and Biotechnology
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• 제17권7호
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• pp.1071-1078
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• 2007

Using a 50-1 airlift bioreactor, for the effective production of tylosin from Streptomyces fradiae TM-224 using raw cornmeal as the energy source, various environmental factors were studied in flask cultures. The maximum tylosin concentration was obtained at $32^{\circ}C$ and pH between 7.0 and 7.5. When seed was inoculated after 24 h of culture, the maximum tylosin concentration, 5.7 g/l, was obtained after 4 days of culture. Various concentrations of raw cornmeal were tested to investigate the optimum initial concentration for the tylosin production. An initial raw cornmeal concentration of 80 g/l gave the highest tylosin concentration, 5.8 g/l, after 5 days of culture. Of the various nitrogen sources, soybean meal and fish meal were found to be the most effective for the production of tylosin. In particular, with the optimal mixing ratio, 12 g/l of soybean meal to 14 g/l of fish meal, 7.2 g/l of tylosin was obtained after 5 days of culture. To compare raw cornmeal and glucose for the production of tylosin in the 50-1 airlift bioreactor for 10 days, fed-batch cultures were carried out under the optimum culture conditions. When raw com meal was used as the energy source, the tylosin production increased with increasing culture time. The maximum tylosin concentration after 10 days of culture was 13.5 g/l, with a product yield from raw cornmeal of 0.123 g/g of consumed carbon source, which was about 7.2 times higher than that obtained when glucose was used as the carbon source.

• 한국항해항만학회:학술대회논문집
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• 한국항해항만학회 2002년도 춘계학술대회논문집
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• pp.21-30
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• 2002

Busan Port which is the representative social overhead capital facilities for international trade of goods has been the driving force for economic development in Korea. Therefore, the central government should play the major role in building a rear road to Busan Port in order that it may function as a mojor port of Northeastern Asia through the systemization of mutual assistance among connected facilities, completely equipped with port-related facilities befitting to the principal port of imports and exports. In this study, the validity of container tax is being examined, analyzing container tax which Is considered as an obstacle to the development of Busan Port and its purpose, and grasping the present conditions by the realistic speculation on container tax issues and its abolition. First, the port rear road as a social overhead capital facilities, which connects port and expressway, should be considered as part of port, and port is social overhead capital invested by government. Second, the susan City imposes on container tax. As a result, a shipper and a shipping company are paying a double charge by paying container tax with port dues. Third, someone argues that port rear road is constructed as container truck generates much traffic, but it is unreasonable to conclude that container truck is seed of traffic jam because according to traffic survey of Busan City, container truck occupies only 1.45% of total traffic Forth, it has bad influence upon the competitiveness of Busan Port as Northeastern logistics base, as other ports who are competing with Busan Port like Singapore. Hong kong, Kobe make their competitiveness strong by decreasing the cost of pore dues

• 한국조경학회지
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• 제22권4호
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• pp.95-117
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• 1995

This study was carried out to investigate Korean native trees in the America landscape through arboretums, the plant inventory of America, computer files for accessions from Korea, source lists of plants and seeds, and seed and plant nursery company. The results were as follows; 1. Korean native trees introduced in America and Canada were 119 species, 116 species were being cultivated in arbrids were being sold in nurseries, new cultivars were selected from 32 species, and hybrids were bred from 5 species. 2. Abies koreana, Malus baccata, and Stewartia koreana were being cultivated in many arboretums and sold in many nurseries. Also there were new hybrids as well as new cultivars. 3. 25 species including Albizzia julibrissin were being cultivated in many arboretums and sold in many nurseries. Also there were new hybrids. 4. Ilex integra and Ilex rotunda were being cultivated in many arboretums and sold in many nurseries. There were no new cultivars, but there were new hybrids. 5. 44 species including Abies holophylla were being cultivated in many arboretums and sold in many nurseries. There were no new cultivars or hybrids. 6. Populus tremula var. davidiana was being cultivated in many arboretums. There were new cultivars of 'Erecta', 'Gigas', 'Pendula', but no new hybrids. They were not sold by any nurseries. 7. 41 species including Abies nephrolepsis were being cultivated in many arboretums. But they were not sold by any nurseries. They didn't have any new cultivars or hybrids. 8. Diospyros kaki 'Kyungsan Bansi', Elaeocarpus sylvestris var. ellipticus, and Pyrus serotina 'Dankae' were not being cultivated in arboretums in America but some nurseries were selling those species as well as new cultivars. 9. Only 3 species (Berchemia berchemiaefolia, Carpinus eximia, and Chionanthus retusus var. coreana which are native Korean plants) were not introduced in America or Canada.

• 화훼연구
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• 제17권3호
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• pp.208-213
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• 2009

1968년 UPOV 발족 이래 회원국들이 증가하고 있으며 품종보호제도가 강화되었다. 우리나라는 UPOV에 50번째 회원국으로 가입하였으며 국내에는 장미와 국화 등 외국으로부터 많은 화훼직물들이 국립종자원에 출원 등록되어 있다. 절화 및 구근 수입국인 우리나라가 외국으로부터 품종을 도입할 경우 로열티가 부담으로 작용한다. 국가별 각 업체별 기본유래품종으로부터 변이체를 발견하였을 때 취급요령과 발견자(또는 재배자)와 육종가와의 관계 등 업계의 정책에 대해서 조사하였다. 그 결과, 대부분의 업체에서 변이 발견자에게는 권리가 없으며 육종가(육종회사)에게 권리가 부여되었다. 둘째 변이 발견자에게 권리는 인정하지만 상업화될 경우 육종가와 상호합의 해야 하는 경우가 있었다. 셋째, 발견자에게 일부 보상제도를 도입되는 경우가 관찰되었다.

• Plant Breeding and Biotechnology
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• 제6권4호
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• pp.354-362
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• 2018

Maize has high food and industrial value, whereas has difficulties in research because of their complex and huge size genome. Nested association mapping (NAM) was constructed to better understand maize genetics. However, most studies were conducted using the reference genome B73, and only a few studies were conducted on tropical maize. Ki3, one of the founder lines of the NAM population, is a tropical maize. We analyzed the genetic characteristics of Ki3 by using RNA sequencing and bioinformatics tools for various genetic studies. As results, a total of 30,526 genes were expressed, and expression profile were constructed. A total of 1,558 genes were differentially expressed in response to drought stress, and 513 contigs of them come from de novo assemblies. In addition, high-density polymorphisms including 464,930 single nucleotide polymorphisms (SNPs), 21,872 multiple nucleotide polymorphisms (MNPs) and 93,313 insertions and deletions (InDels) were found compared to reference genome. Among them, 15.0 % of polymorphisms (87,838) were passed non-synonymous test which could alter amino acid sequences. The variants have 66,550 SNPs, 5,853 MNPs, and 14,801 InDels, also proportion of homozygous type was higher than heterozygous. These variants were found in a total of 15,643 genes. Of these genes, 637 genes were found as differentially expressed genes (DEGs) under drought stress. Our results provide a genome-wide analysis of differentially expressed genes and information of variants on expressed genes of tropical maize under drought stress. Further characterization of these changes in genetic regulation and genetic traits will be of great value for improvement of maize genetics.

• 아태비즈니스연구
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• 제13권3호
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• pp.19-34
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• 2022

Purpose - The purpose of this study is to examine whether a psychological concept enhances healthcare users' service experience. Specifically, the study proposes and empirically examines a model of perceived control in which the user's sense of control is postulated as exerting positive influences upon his/her motivation, self-efficacy associated with his/her role as a patient, and satisfaction with his/her medical service experience. Methodology - Data were collected by a professional research company, using an online survey method. Participants of the study included adults nineteen years or older who had visited a medical service institute at least once during the previous one-year period. For the test of the research hypotheses, structural equation modeling using AMOS was used. Findings - Findings of this study denote a unique insight into the users' comprehension of medical service experiences and their behaviors. First, the concept of perceived control is identified as a factor that enhances the quality of individuals' medical service experiences. A sense of control directly influences medical users' self-efficacy to comply with doctor's recommendations, their motivation to comply with doctor's recommendations, and their satisfaction with the medical service experience. Second, one's perceived self-efficacy is found to exert positive influences upon both motivation and satisfaction. Third, one's motivation to comply with the doctor's recommendation is found to exert a positive influence upon one's satisfaction. Additionally, perceived control is found to exert an indirect influence upon medical service users' satisfaction through the mediation of both self-efficacy and motivation. Research Implications - The findings of the study support the notion that perception of control among medial service users enhances their service experience as patients. The main thrust of this study suggests that it is necessary for healthcare practitioners to consider implementing service encounter strategies that purposefully enhance the sense of control among their patients. The identification of significant inter-relationships among perceived control, motivation, self-efficacy, and satisfaction among medical service customers should also serve as a meaningful seed for further research pursuits.

• 원예과학기술지
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• 제33권6호
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• pp.941-946
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• 2015

호접란 신품종 '화수 5205'는 2002년 경북대에서 Phal. Happy Valentin과 Dtps. Happy Rose를 모부본으로 교배하여 육성한 $F_1$개체 중에서 육성하였다. 꽃은 선명하고 진한 적색의 대륜계이다. 2003-2004년 2년에 걸쳐 실생 300개체를 양성하여 이들 중에서 영양생장과 개화특성이 우수한 개체 02-05-205를 선발하였다. 2004년과 2005년에 1차, 2차 특성검정을 통하여 품종의 안정성과 균일성을 확인하고 '화수 5205'로 명명하였다. '화수 5205'는 화색이 선명하고 진한 적색(PN78B)이며, 화형은 안아피기로 안정되어 있다. 꽃의 길이와 폭은 각각 9.2, 12.0cm로 대형이다. 잎의 길이와 폭은 각각 24.3, 8.5cm이며 엽형은 수평이다. 기내증식율이 높고 변이가 거의 없으며 영양생장 우수하여 재배관리가 용이하다. 2009년 12월 1일 품종등록(등록번호 제2915호)하여 종자산업법에 의해 보호받고 있다.

• 아시안잔디학회지
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• 제19권2호
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• pp.141-149
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• 2005

본 연구는 국내외 잔디 특허 및 품종 관련특허의 현황을 분석하여 잔디 관련 특허기술의 현황과 사업화에 대한 정보를 제공하고자 하였다. 연구의 시간적 범위는 1948년 11월 20일년부터 2005년 11월 30일까지 57년간 공개된 잔디관련 특허출원 및 등록 기술을 대상으로 분석하였다. 연구의 내용적 범위는 잔디 산업분야 중 잔디 품종 및 생산에 관련된 특허를 중심으로 분석하였다. 특허 자료의 분석은 한국특허기술정보원(www.kipris.or.kr) 홈페이지를 통하여 분석하였으며 그 결과를 요약하면 다음과 같다. 1. 잔디 산업 분야별 국내 특허출원 건수는 잔디비료에 대한 내용이 866건으로 가장 많았다. 잔디 비료에 관한 특허 출원 내용은 잔디 액비, 기비, 추비에 관한 내용이었다. 2. 국가별 특허출원 총 건수는 일본이 1,565건으로 미국의 1,427건보다 많았다. 특히 외국의 특허출원은 우리나라와 달리 잔디생산에 관련한 품종개량, 생산시스템 등에 관한 내용이 많은 것으로 조사되었다. 3. 잔디 산업 주체별 특허출원 건수는 기업이 2,328건으로 가장 높았으며 다음이 개인, 연구소, 교수 순으로 나타났다. 특히 연구의 주체인 공공기관과 대학교수의 특허출원 건수가 상대적으로 낮았다. 4. 국내 특허 등록기술의 사업화 현황은 특허 출원 기술의 $96\%$가 활용되지 않는 것으로 나타나 실용적인 기술에 대한 권리확보가 필요할 것으로 판단된다. 5. 국내 잔디 특허 품종은 건희, 건우, 안양중지, 세녹 등이었으며, 국외 품종은 제니스, 미야코 등이며 안양중지, 제니스, 미야코, 건희 등이 상업화되고 있는 것으로 나타났다.

• 원예과학기술지
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• 제19권2호
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• pp.140-144
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• 2001

고랭지에 적합한 신소득 작목을 개발하고자 최근에 수요가 증가하고 있는 쌈채소중에서 적근대 등 17종을 공시하였다. 파종은 128공 플러그트레이에 30일간 육묘하였으며 비가림하우스에 정식하여 생육, 특성, 수량 등 제반 수량형질과 쌈으로서의 재배 및 이용 가능성을 조사하였다. 정식시 묘소질은 공시작목 17종 모두 묘상 및 본포 생육은 고랭지 환경조건에서 순조로운 양상을 보였다. 이들 중에서 부추, 삼엽채 및 로메인상추 등의 첫수확은 8월 상중순이었으나 나머지 작목은 7월 10일이었다. 삼엽채 등을 비롯한 11종은 7월 중순-8월 하순에 추대하여 대부분 개화에 도달하였다. 로메인상추, 화채 등 일시수확하는 작목을 제외한 전 작목 모두 월별 수확이 진행됨에 따라 상품엽의 크기가 점차 감소하였으며 9월 수확시에 그 감소 정도가 다소 컸다. 7-9월의 상품수량은 적근대, 적겨자, 겨자채, 청경채, 다채, 로메인상추, 적엽치커리, 적치커리, 녹엽치커리 등 9작목이 비교적 높은 수준이었다.

• 원예과학기술지
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• 제33권1호
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• pp.149-154
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• 2015

신품종 Phalaenopsis 'SM 333'은 2002년 상미원에서 Phalaenopsis 'Odoriko'와 Phalaenopsis 'Be Tris'를 모부본으로 인공교배하여 육성한 $F_1$ 개체 중에서 선발한 핑크 소형 다화 분지성이다. 2003년부터 2004년까지 2년에 걸쳐 실생 300개체를 양성하여 이들 중에서 영양생장과 개화특성이 우수한 개체 02-03-33을 선발하였다. 2004년과 2005년에 1차, 2차 특성검정을 통하여 품종의 안정성과 균일성을 확인하고 'SM 333'으로 명명하였다. 'SM 333'은 화색이 화사하고 선명한 핑크(RP69D)이며, 화형은 앉아피기로 안정되어 있다. 꽃의 길이와 폭은 각각 5.0, 5.8cm로 소형이다. 복총상화서로 볼륨이 있으며 소형 캐주얼분화에 적합하다. 잎의 길이와 폭은 각각 20.8, 6.5cm이며 엽형은 수평이다. 기내증식율이 높고 변이가 거의 없으며 영양생장 우수하여 재배관리가 용이하다. 2009년 12월 1일 품종등록(등록번호 제2916호)하여 종자산업법에 의해 보호받고 있다.