• 제목/요약/키워드: Seed Culture

검색결과 558건 처리시간 0.035초

Temperature ranges for survival and growth of juvenile Saccharina sculpera (Laminariales, Phaeophyta) and applications for field cultivation

  • Kim, Soo Hong;Kim, Young Dae;Hwang, Mi Sook;Hwang, Eun Kyoung;Yoo, Hyun Il
    • ALGAE
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    • 제36권4호
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    • pp.231-240
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    • 2021
  • Saccharina sculpera is highly valued for human consumption and value-added products. However, natural resources of this kelp have decreased sharply and it is in danger of extinction. Resources recovery through cultivation is being trialed to enable the sustainable use of this species. In this study, the temperature range for survival and optimal growth of juvenile S. sculpera was identified and applied to field cultivation. This study investigated the survival and growth of juvenile S. sculpera under six temperatures (i.e., 5, 10, 15, 16, 18, and 20℃) and two light intensities (i.e., 20 and 40 µmol photons m-2 s-1) in an indoor culture experiment. In these experiments, the blade length decreased at 16℃ under the both light intensities. The thalli died at 20℃ and 20 µmol photons m-2 s-1, and at 18-20℃ and 40 µmol photons m-2 s-1. During the field cultivation, early growth of S. sculpera was highest at the 5 m depth and growth decreased as the water depth increased. When the initial rearing depth was maintained without adjustment throughout the cultivation period (from December to October), all the cultivated S. sculpera plants died during August and September. However, S. sculpera plants lowered from 5 to 15 m and grew to 90.8 ± 13.1 cm in July. The seawater temperature at 15 m depth was similar to the upper level of thermal tolerance demonstrated by juvenile S. sculpera in the indoor culture experiments (16℃ or lower). The plants were subsequently lowered to 25 m depth in August, which eventually led to their maturation in October. The present study confirmed that improved growth rates and a delay in biomass loss can be achieved by adjusting the depth at which the seaweeds are grown during the cultivation period. These results will contribute to the establishment of sustainable cultivation systems for S. sculpera.

Plant Regeneration via Organogenesis from Seed Explants in Red Pepper (Capsicum annuum L.)

  • Lee, Kwang-Woong
    • Journal of Plant Biology
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    • 제39권3호
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    • pp.167-172
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    • 1996
  • Efficient plant regeneration has been achieved via organogenesis in the red pepper plant (Capsicum annum L.). Shoots were induced from seed explants of cultivar 'Friendship' on Murashige and Skoog's (MS) basal medium supplemetned with; NAA or IAA, and BAP or zeatin. Seed explants on the medium supplemented with 0.1-0.3 mg/L IAA and 2-5 mg/L zeatin for 2 weeks vigorously formed normal shoots in more than 90% of the explants. When these were transferred to MS medium containing 0.5-1.0 mg/L GA, 90-100% of the shoots have elongated within 1-2 weeks. The elongated shoots rooted in media supplemented with 0.3 mg/L NAA. It was revealed that this method is very rapid and efficient regeneration system for red pepper and regenerated plants can be obtained after only 5-6 weeks of culture.

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Influence of Cultivation Period on Seed Bulb Production of Korean Native Allium wakegi Araki

  • Jo Man-Hyun;Ham In-Ki;Park Sang-Kyu;Lee Mi-Ae;Kwon Kyeong-Hak;Woo In-Shik
    • Plant Resources
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    • 제8권1호
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    • pp.48-51
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    • 2005
  • Optimmum cultivation period was determined for producing seed bulb of Korean native Allium wakegi Araki in vitro in hydroponic culture. The growth gradually increased during cultivation period. In general, plants grown for 5 months produced significantly the highest bulb number and bulb fresh weight per plant. Raising the cultivation period from 1 to 5 months remarkably increased seed bulb yield.

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땅콩의 초형별 생태적 특성에 관한 연구 III. 초형별 건물생산능력과건물 분배율 (Studies on the Ecological Characteristics for the Plant Types in the Peanut(Arachis hypogaea L.) III. The Differences in Dry Matter Production and Distribution Ratio of Dry Matter Produced among Plant Types)

  • 이정일;박용환;박연규
    • 한국작물학회지
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    • 제30권1호
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    • pp.63-68
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    • 1985
  • 땅콩의 초형, 재배양식에 따른 건물생산능력 및 건물분배율을 구명하고저 virginia소립종, virginia대립종, spanish, valencia, shinpung의 5초형에 속하는 10품종을 공시, 재배양식 2수준(피복, 무피복)으로 하여 시험을 실시하였던 바 그 결과를 요약하면다음과 같다. 1. 건물생산량은 피복재배의 경우 virginia-large seed 1,556g/$m^2$, virginia-small seed 1,347g/$m^2$, shinpung 1,307g/$m^2$, spanish 1,170g/$m^2$, valencia 1,120g/$m^2$이었으며 무피복재배에서는 virginia-large seed 1,389g/$m^2$, virginia-small seed 1,267 g/$m^2$, shinpung 1,192g/$m^2$, spanish 1,066g/$m^2$, valencia 1,035g/$m^2$였다. 2. 최대건물생장속도(CGR)는 virginia-large seed 19.61~20.03g/$m^2$/day, virginia-small seed 18.22~23.4lg/$m^2$/day, shinpung 16.33~19.77g/$m^2$/day, spanish 13.86~16.28g/$m^2$/day, valencia 14.97~16.25g/$m^2$/day였다. 3. 엽면적지수(LAI)는 무피복재배보다 피복재배에서 높았는데, 피복재배의 경우 virginia-large seed 17.2 >virginia-small seed 7.0 > spanish 5.5 > valencia=shinpung 5.4순이었고, 무피복재배에서는 virginia-large seed 6.1 > virginia - small seed 5.8 > spanish 5.0 > valencia 4.9 > shinpung 4.4 순으로 virginia-large seed가 피복, 무피복재배에서 모두 높았다. 4. 경, 엽, 근의 건물분배율은 생육이 진전됨에 따라 감소하였고 협실의 건물분배율은 증가하는 경향으로등숙초기(7월말)에 있어서 피복재배의 경우 shinpung 86%> virginia-small seed 64%>virginia-large seed 63%>spanish 45%>valencia 44%순이었으며 무피복재배에서는 shinpung=spanish=valencia 60%> virginia - large seed 45%> virginia-small seed 40%순으로 shinpung type이 등숙초기에 건물분배율이 가장 높았다.

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이탈리안 라이그래스의 종자배양에 있어서 식물체 재분화에 미치는 몇 가지 요인 (Factors Affecting Plant Regeneration from Seed-Derived Calli in Italian ryegrass (Lolium multiflorum Lam.))

  • 이효신;강경민;조진기
    • 식물조직배양학회지
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    • 제28권6호
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    • pp.323-328
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    • 2001
  • 이탈리안 라이그래스의 종자배양에서 캘러스 형성 및 식물체 재분화 체계를 확립하기 위하여 기본배지와 탄소원의 종류별 식물체 재분화 정도 및 배양효율의 품종 간 차이 등에 대한 실험을 수행하여 얻어진 결과를 요약하면 다음과 같다. 기본배지의 종류에 따른 캘러스 형성 및 식물체 재분화율은 MS 배지가 N6 배지나 B5 배지보다 높게 나타났다. 배지 내에 첨가되는 탄소원의 종류에 따른 식물체 재분화율은 sucrose가 maltose보다 효과적이었다. 이탈리안 라이그래스의 종자배양에 있어서 모식물의 genotype에 따라 식물체 재분화 능력은 큰 차이를 보였으며, 공시품종 중 'Rio' 와 'Jeanne' 의 재분화 능력이 각각 38%와 56%로 가장 높았다.

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Production of a Phytotoxic Compound, 3-Phenylpropionic Acid by a Bacterial Endophyte, Arthrobacter humicola YC6002 Isolated from the Root of Zoysia japonica

  • Chung, Eu-Jin;Park, Joo-Hwang;Park, Tae-Soon;Ahn, Jong-Woong;Chung, Young-Ryun
    • The Plant Pathology Journal
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    • 제26권3호
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    • pp.245-252
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    • 2010
  • An endophytic bacterial strain, Arthrobacter humicola YC6002, was isolated from a surface sterilized root of Korean turf grass (Zoysia japonica) collected from Jinju, Korea. This strain showed inhibitory effect on germination and shoot growth of radish. The inhibition of germination and shoot growth of radish seeds varied depending on the age of culture and the temperature at which it was incubated. The culture filtrate of 1/10-strength Tryptic Soy Broth medium, incubated for 48 hours at $30^{\circ}C$, showed the highest inhibitory effect on radish seed germination and shoot growth (92% inhibition as compared to control). The active compound with seed germination and shoot growth inhibition was purified and identified as 3-phenylpropionic acid. The purified compound had 53% and 93% inhibitory effect on seed germination and shoot growth of radish for 500 and 1000 ppm solutions, respectively.

설기떡의 품질향상을 위한 부재료 첨가의 효과 (Effect of Addition of Minor ingredients for the Quality Characteristics of Sulgiduk)

  • 이가순;이주찬;이종국;박원종
    • 한국식생활문화학회지
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    • 제16권5호
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    • pp.399-406
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    • 2001
  • This study was carried out to investigate the effects of addition ratio(0, 5, 10 and 20%) of various minor ingredients(prosomillet, glutinous barley, oat, wheat, yellow and black soybean, safflower seed, chocolate etc.) on mechanical and sensory characteristics of Sulgiduk. In mechanical evaluation, hardness, cohesiveness and gumminess were increased with increasing the content of prosomillet, glutinous barley, oat and wheat to be added, and did not show any relationship the content of them to be added on yellow and black soybean, safflower seed and chocolate. In sensory evaluation, quality characteristics of Sulgiduk added with prosomillet, oat, yellow and black soybean and chocolate with $5{\sim}10%$, respectively was better than those of Sulgiduk without the minor ingredients. Change of hardness on Sulgiduk added with oat, yellow soybean, black soybean and chocolate was lower than those of Sulgiduk without them during storage of room temperature.

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구약감자의 종서처리 및 재배방법이 수량에 미치는 영향 (Effects of Seed Tuber Processing and Cultural methods on Tuber yield of Amorphophallus Konjac. K.)

  • 이희덕
    • 한국작물학회지
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    • 제37권2호
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    • pp.117-122
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    • 1992
  • 본실험은 구약자의 수량증수를 위해 파종기별 저장 방법과 재배양식 절단방법과 자구의 크기, 차광효과를 구명코자 시험하였던바 결과를 요약하면 다음과 같다. 1. 파종기에 있어서 4월 20일 전파종양식에서보다 조기파종 4월 5일이 수량성이 증가하였다. 2. 저장방법시험에서는 일반저장 PC film에서 수량성이 가장 높았고 입모율은 우수하였으나, 반면 구약감자 출현율은 육아재배 PE멀칭과 PE터널에서 20일 촉진할수 있으나 특히 입모율은 주야간 기온차로 특별한 재배관리가 요망된다. 3. 자구절단방법 2절, 4절은 관행보다 자구수가 증가하였고 종자소요량과 대량번식이 유리하였다. 4. 구약감자 재배는 자연광(관행)보다 30%, 50% 차광처리에서 생육특성과 종자수량이 증가하였다.

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Addition Effect of Seed-associated or Free Linseed Oil on the Formation of cis-9, trans-11 Conjugated Linoleic Acid and Octadecenoic Acid by Ruminal Bacteria In Vitro

  • Wang, J.H.;Song, M.K.;Son, Y.S.;Chang, M.B.
    • Asian-Australasian Journal of Animal Sciences
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    • 제15권8호
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    • pp.1115-1120
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    • 2002
  • The effects of seed-associated or free linseed oil on fermentation characteristics and long-chain unsaturated fatty acids composition, especially the formation of conjugated linoleic acid (CLA) and octadecenoic acid (trans-11 $C_{18:1}$, $t-C_{18:1}$) by mixed ruminal bacteria were examined in vitro. Concentrate (1% of culture solution, w/v, as-fed basis) with ground linseed (0.6% of culture solution, w/v, DM basis) or linseed oil as absorbed onto ground alfalfa hay was added to 600 ml mixed solution consisting of strained rumen fluid and artificial saliva at the ratio of 1:1 in a glass culture jar. The culture jar was covered with a glass lid with stirrer, and placed into a water-bath ($39^{\circ}C$) and incubated anaerobically up to 24 h. Seed-associated or free linseed oil did not significantly affect the pH and ammonia concentration in the culture solution. Molar percent of acetate tended to increase while that of propionate decreased with the addition of free oil treatment throughout the incubation. Differences in bacterial number were relatively small, regardless of the form of supplements. Decreasing trends in the compositions of linoleic acid ($C_{18:2}$) and linolenic acid ($C_{18:3}$) but increasing trends of stearic acid ($C_{18:0}$), $t-C_{18:1}$ and CLA compositions were found from culture contents up to 12h incubation when incubated with both ground linseed and linseed oil. The compositions of $C_{18:0}$, $C_{18:2}$ and $C_{18:3}$ were greater but those of oleic acid ($C_{18:1}$), $t-C_{18:1}$ and CLA were smaller in a culture solution containing ground linseed than those containing linseed oil. The ratio of $t-C_{18:1}$ to CLA was lower in the culture solutions containing linseed oil up to 12h incubations as compared to those containing ground linseed.

명아주(Chenopodium album)와 바랭이( Digitaria sanguinalis)의 경쟁이 에너지 분해에 미치는 효과 (The Effect of Energy Allocation on Competition of Chenopodium album and Digitaria sanguinalis)

  • Park, Bong-Kyu;Kim, Ok-Kyung
    • The Korean Journal of Ecology
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    • 제9권1호
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    • pp.73-78
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    • 1986
  • The effects of density and soil nutrient regime on competition between individuals in pure and mixed populations of two annuals. Chenopodium album and Digitaria sanguinalis were investigated at the level of enegy allocation. Seedling emergence of two species was extremely high (>90%) in both pure and mixed culture irrespective of the density and nutrient regime, but percentage of seedling establishment or fertile plant became gradually low with increasing density owing to 'self-thinning'. The mean plant dry weight was significantly reduced with increasing density and decreasing soil fertility. The dry matter production of D. sanguinalis in mixture was markedly greater than in pure culture under medium and high density. Also, as considered the number of seed production as reproductive allocation, relationship between them and the number of seed production per plant showed a similar tendency. Therefore, these results indicated that D. sanguinalis had a greater competitive advantage than C. album and energy allocations to variious organs were regulated by plastic response rather than determined genetically.

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