• ALGAE
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• v.36 no.4
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• pp.231-240
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• 2021

Saccharina sculpera is highly valued for human consumption and value-added products. However, natural resources of this kelp have decreased sharply and it is in danger of extinction. Resources recovery through cultivation is being trialed to enable the sustainable use of this species. In this study, the temperature range for survival and optimal growth of juvenile S. sculpera was identified and applied to field cultivation. This study investigated the survival and growth of juvenile S. sculpera under six temperatures (i.e., 5, 10, 15, 16, 18, and 20℃) and two light intensities (i.e., 20 and 40 µmol photons m^-2 s^-1) in an indoor culture experiment. In these experiments, the blade length decreased at 16℃ under the both light intensities. The thalli died at 20℃ and 20 µmol photons m^-2 s^-1, and at 18-20℃ and 40 µmol photons m^-2 s^-1. During the field cultivation, early growth of S. sculpera was highest at the 5 m depth and growth decreased as the water depth increased. When the initial rearing depth was maintained without adjustment throughout the cultivation period (from December to October), all the cultivated S. sculpera plants died during August and September. However, S. sculpera plants lowered from 5 to 15 m and grew to 90.8 ± 13.1 cm in July. The seawater temperature at 15 m depth was similar to the upper level of thermal tolerance demonstrated by juvenile S. sculpera in the indoor culture experiments (16℃ or lower). The plants were subsequently lowered to 25 m depth in August, which eventually led to their maturation in October. The present study confirmed that improved growth rates and a delay in biomass loss can be achieved by adjusting the depth at which the seaweeds are grown during the cultivation period. These results will contribute to the establishment of sustainable cultivation systems for S. sculpera.

• Journal of Plant Biology
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• v.39 no.3
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• pp.167-172
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• 1996

Efficient plant regeneration has been achieved via organogenesis in the red pepper plant (Capsicum annum L.). Shoots were induced from seed explants of cultivar 'Friendship' on Murashige and Skoog's (MS) basal medium supplemetned with; NAA or IAA, and BAP or zeatin. Seed explants on the medium supplemented with 0.1-0.3 mg/L IAA and 2-5 mg/L zeatin for 2 weeks vigorously formed normal shoots in more than 90% of the explants. When these were transferred to MS medium containing 0.5-1.0 mg/L GA, 90-100% of the shoots have elongated within 1-2 weeks. The elongated shoots rooted in media supplemented with 0.3 mg/L NAA. It was revealed that this method is very rapid and efficient regeneration system for red pepper and regenerated plants can be obtained after only 5-6 weeks of culture.

• Plant Resources
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• v.8 no.1
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• pp.48-51
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• 2005

Optimmum cultivation period was determined for producing seed bulb of Korean native Allium wakegi Araki in vitro in hydroponic culture. The growth gradually increased during cultivation period. In general, plants grown for 5 months produced significantly the highest bulb number and bulb fresh weight per plant. Raising the cultivation period from 1 to 5 months remarkably increased seed bulb yield.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.30 no.1
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• pp.63-68
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• 1985

This study was conducted to investigate the dry matter production and distribution ratio of dry matter produced for each of 5 botanical types (Virginia-Small Seed, Virginia-Large Seed, Spanish, Valencia, Shinpung) of Peanut (Arachis hypogaea L.) in Peanut culture limiting region. The total dry weight increased in order of Virginia-Large seed, Virginia- Small Seed, Shinpung, Spanish, Valencia type. The maximum Crop growth rates (Cmax) were Virginia-Small seed 18.22-23.41 g/㎡/day, Virginia-Large seed 19.61-20.03 g/㎡/day, Shinpung 16.33-19.77 g/㎡/day, Spanish 13.86-16.28 g/㎡/day, Valencia 13.97-16.25g/㎡/day, respectively. LAI showed the high value at vinyl-mulching than non-mulching. In the early filling stage, distribution ratio of dry matter produced showed the highest at the shinpung type than the other types.

• Korean Journal of Plant Tissue Culture
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• v.28 no.6
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• pp.323-328
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• 2001

In order to optimize the conditions of callus induction and plant regeneration in seed culture of Italian ryegrass, the effects of basic medium and carbon sources on seed culturability and genotypic difference of plant regenerability were investigated. MS medium was better than N6 and B5 medium in enhancing callus growth and plant regeneration. Sucrose was superior to maltose in plant regeneration as carbon source in the medium. The genotypic difference in plant regenerability was obvious among four cultivars of Italian ryegrass tested. 'Rio' and 'Jeanne' showed to have higher regenerability with the frequency of 38% and 56%, respectively.

• The Plant Pathology Journal
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• v.26 no.3
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• pp.245-252
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• 2010

An endophytic bacterial strain, Arthrobacter humicola YC6002, was isolated from a surface sterilized root of Korean turf grass (Zoysia japonica) collected from Jinju, Korea. This strain showed inhibitory effect on germination and shoot growth of radish. The inhibition of germination and shoot growth of radish seeds varied depending on the age of culture and the temperature at which it was incubated. The culture filtrate of 1/10-strength Tryptic Soy Broth medium, incubated for 48 hours at $30^{\circ}C$, showed the highest inhibitory effect on radish seed germination and shoot growth (92% inhibition as compared to control). The active compound with seed germination and shoot growth inhibition was purified and identified as 3-phenylpropionic acid. The purified compound had 53% and 93% inhibitory effect on seed germination and shoot growth of radish for 500 and 1000 ppm solutions, respectively.

• Journal of the Korean Society of Food Culture
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• v.16 no.5
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• pp.399-406
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• 2001

This study was carried out to investigate the effects of addition ratio(0, 5, 10 and 20%) of various minor ingredients(prosomillet, glutinous barley, oat, wheat, yellow and black soybean, safflower seed, chocolate etc.) on mechanical and sensory characteristics of Sulgiduk. In mechanical evaluation, hardness, cohesiveness and gumminess were increased with increasing the content of prosomillet, glutinous barley, oat and wheat to be added, and did not show any relationship the content of them to be added on yellow and black soybean, safflower seed and chocolate. In sensory evaluation, quality characteristics of Sulgiduk added with prosomillet, oat, yellow and black soybean and chocolate with $5{\sim}10%$, respectively was better than those of Sulgiduk without the minor ingredients. Change of hardness on Sulgiduk added with oat, yellow soybean, black soybean and chocolate was lower than those of Sulgiduk without them during storage of room temperature.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.37 no.2
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• pp.117-122
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• 1992

This experiment was conducted to determine tuber yield increase of Konjac by sowing time, seed tuber split method and cultural methods. Tuber yield per unit area was generally increased in early planting than conventional planting time. Tuber yield of polyethylene film mulching culture among storage methods was high, while the emergence rate of konjac for seedling plus PE mulching, and tunnel culture were prompted by 20 days, and especially seedling was to be greatly controlled due to temperature difference of the day and night at emergence. All of the cutting methods(two and four split method) were higher than conventional method because of increasing number of bulblet, accordingly, that method using seed tuber was profitable for mass propagation. Both botanical characteristics and tuber yield tend to be increased at 30 to 50 percent shading than natural condition.

• Asian-Australasian Journal of Animal Sciences
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• v.15 no.8
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• pp.1115-1120
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• 2002

The effects of seed-associated or free linseed oil on fermentation characteristics and long-chain unsaturated fatty acids composition, especially the formation of conjugated linoleic acid (CLA) and octadecenoic acid (trans-11 $C_{18:1}$, $t-C_{18:1}$) by mixed ruminal bacteria were examined in vitro. Concentrate (1% of culture solution, w/v, as-fed basis) with ground linseed (0.6% of culture solution, w/v, DM basis) or linseed oil as absorbed onto ground alfalfa hay was added to 600 ml mixed solution consisting of strained rumen fluid and artificial saliva at the ratio of 1:1 in a glass culture jar. The culture jar was covered with a glass lid with stirrer, and placed into a water-bath ($39^{\circ}C$) and incubated anaerobically up to 24 h. Seed-associated or free linseed oil did not significantly affect the pH and ammonia concentration in the culture solution. Molar percent of acetate tended to increase while that of propionate decreased with the addition of free oil treatment throughout the incubation. Differences in bacterial number were relatively small, regardless of the form of supplements. Decreasing trends in the compositions of linoleic acid ($C_{18:2}$) and linolenic acid ($C_{18:3}$) but increasing trends of stearic acid ($C_{18:0}$), $t-C_{18:1}$ and CLA compositions were found from culture contents up to 12h incubation when incubated with both ground linseed and linseed oil. The compositions of $C_{18:0}$, $C_{18:2}$ and $C_{18:3}$ were greater but those of oleic acid ($C_{18:1}$), $t-C_{18:1}$ and CLA were smaller in a culture solution containing ground linseed than those containing linseed oil. The ratio of $t-C_{18:1}$ to CLA was lower in the culture solutions containing linseed oil up to 12h incubations as compared to those containing ground linseed.

• The Korean Journal of Ecology
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• v.9 no.1
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• pp.73-78
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• 1986

The effects of density and soil nutrient regime on competition between individuals in pure and mixed populations of two annuals. Chenopodium album and Digitaria sanguinalis were investigated at the level of enegy allocation. Seedling emergence of two species was extremely high (>90%) in both pure and mixed culture irrespective of the density and nutrient regime, but percentage of seedling establishment or fertile plant became gradually low with increasing density owing to 'self-thinning'. The mean plant dry weight was significantly reduced with increasing density and decreasing soil fertility. The dry matter production of D. sanguinalis in mixture was markedly greater than in pure culture under medium and high density. Also, as considered the number of seed production as reproductive allocation, relationship between them and the number of seed production per plant showed a similar tendency. Therefore, these results indicated that D. sanguinalis had a greater competitive advantage than C. album and energy allocations to variious organs were regulated by plastic response rather than determined genetically.