• 환경영향평가
• /
• 제26권3호
• /
• pp.207-216
• /
• 2017

오염퇴적물의 생물영향평가를 위해 다양한 생물검정 연구가 수행되고 있다. 퇴적물에 직접 노출시키는 평가 방법은 실험과정 동안 섭식 및 섭식에 의한 생물의 영향을 배제할 수 없으며 관찰을 위한 실험 생물이 대형생물 또는 저서성 생물에 제한되는 등의 한계가 있다. 본 실험에서는 짧은 생활사를 가지며 많은 연구에서 독성 결과가 축적되어 있는 요각류와 퇴적물의 용출수를 이용해 생물영향평가의 가능성을 확인하고자 하였다. 오염의 정도가 다른 두 정점의 퇴적물 용출수에 해산 요각류를 노출 시켜 개체 및 분자 수준에서 관찰되는 변화를 측정한 결과 해산 요각류의 유생의 성장과 분자생체지표의 발현에서 오염된 퇴적물의 용출수에서 대조군과 유의한 차이를 보였다. 분자생체지표의 발현은 용출수의 희석 정도와 노출 시간에 의존적인 경향을 나타내 용출수를 이용한 생물영향평가의 가능성을 보여주었다. 본 논문 결과를 바탕으로 퇴적물의 오염 및 생물영향평가에 있어 용출수 노출시험이 오염물질의 정량적 분석결과를 보완할 수 있는 방법으로 이용될 수 있을 것을 확인하였으며 향후 많은 자료의 축적과 활용성에 대한 평가 및 기준이 제시되어야 한다고 제안한다.

• Fisheries and Aquatic Sciences
• /
• 제8권1호
• /
• pp.27-33
• /
• 2005

This is a subsequent study to our previous finding that Pacific oysters, Crassostrea gigas, gained a so-called upper plateau concentration, around 30,000 ng/g dry weight digestive gland for benzo(a)pyrene, showed reproductive behavior but produced their ensuing reproductive outputs damaged. A serial dilution of sediment elutriates from Jinhae Bay, Korea, where pollution was progressive, were exposed to gametes of the Pacific oyster for 0, 5, 10, 20, 30, and 60 min to detail the pollutant effects on very young specimens. There was an apparent critical dilution over which adverse effects are evident. This was $10\%$ of the present sediment elutriate, corresponding to 0.3 ng/g on the basis of total polycyclic aromatic hydrocarbons (PAHs) for the oyster. Within the dilution the embryonic development was not influenced by the duration of exposure to its gamete stage. At higher dilutions over the critical dilution, occurrence of abnormality increased dependent on the pollutant dilution and the duration of exposure. Similar trends were also found in larval mortality. However, overall, the chemical toxicity was more significant to morphogenesis than to survival, suggesting a potential recruitment of the pollutants-induced abnormal larvae in the wild population to threaten the population integrity.

• 한국양식학회지
• /
• 제18권3호
• /
• pp.200-206
• /
• 2005

We previously pointed out that the polluted sediment elutriate manifestly affected the early events of reproductive outputs in the Pacific oysters, Crassostrea gigas. A serial dilution of priority xenobiotic sediment elutriates determined by gas chromatography/mass spectrometry (GC/MS) were exposed to gametes of the oyster with different stress burdens to detail the maternal stress transfer to its reproductive outputs. There was an apparent critical concentration over which survival and morphogenesis were significantly affected with more profound damage in morphogenesis. The critical concentration which drives mortality and abnormal morphogenesis of the larvae corresponded to a dilution between 10 and 20% of our elutriate. The adverse effects of the early lives by the xenobiotic exposure over the critical concentration were magnified by the maternal stress from the exposed benzo(a)pyrene (BaP), one of the priority polyaromatic hydrocarbons (PAHs) during the maturation condition. These results indicate that maternal transfer of the xenobiotic compounds from oysters living in the contaminated location might represent a significant adverse effect to their larval population of wild seeds.