• Molecules and Cells
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• 제40권9호
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• pp.643-654
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• 2017

Autophagy is a degradation pathway in eukaryotic cells in which aging proteins and organelles are sequestered into double-membrane vesicles, termed autophagosomes, which fuse with vacuoles to hydrolyze cargo. The key step in autophagy is the formation of autophagosomes, which requires different kinds of vesicles, including COPII vesicles and Atg9-containing vesicles, to transport lipid double-membranes to the phagophore assembly site (PAS). In yeast, the cis-Golgi localized t-SNARE protein Sed5 plays a role in endoplasmic reticulum (ER)-Golgi and intra-Golgi vesicular transport. We report that during autophagy, sed5-1 mutant cells could not properly transport Atg8 to the PAS, resulting in multiple Atg8 dots being dispersed into the cytoplasm. Some dots were trapped in the Golgi apparatus. Sed5 regulates the anterograde trafficking of Atg9-containing vesicles to the PAS by participating in the localization of Atg23 and Atg27 to the Golgi apparatus. Furthermore, we found that overexpression of SFT1 or SFT2 (suppressor of sed5 ts) rescued the autophagy defects in sed5-1 mutant cells. Our data suggest that Sed5 plays a novel role in autophagy, by regulating the formation of Atg9-containing vesicles in the Golgi apparatus, and the genetic interaction between Sft1/2 and Sed5 is essential for autophagy.

• Journal of Dental Anesthesia and Pain Medicine
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• 제21권5호
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• pp.461-469
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• 2021

Background: The global population is aging rapidly, and accordingly, the number of patients with dementia is increasing every year. Although the need for dental treatment increases for various reasons in patients with dementia, they cannot cooperate during dental treatment. Therefore, behavioral management, including sedation (SED) or general anesthesia (GA), is required for patients with dementia. Thus, this study aimed to investigate the trends and effects of SED or GA in patients with dementia undergoing dental treatment in South Korea based on the Korean National Health Insurance claims data. Methods: This study utilized customized health information data provided by the Health Insurance Review and Assessment Service. Among patients with records of using sedative drugs during dental treatment from January 2007 to September 2019, patients with the International Classification of Diseases-10 code for dementia (F00, F01, F02, F03, and G30) were selected. We then analyzed the full insurance claims data for dental care. Age, sex, sedative use, and dental treatment of patients were analyzed yearly. In addition, the number of cases of GA or SED per year was analyzed, and changes in behavioral management methods with increasing age were investigated. Results: Between January 2007 and September 2019, a total of 4,383 (male, 1,454; female, 2,929) patients with dementia received dental treatment under SED or GA. The total number of SED and GA cases were 1,515 (male, 528 ; female, 987 ) and 3,396 (male, 1,119 ; female, 2,277) cases, respectively. The total number of cases of dental treatment for 4,383 patients with dementia was 153,051 cases, of which 2.22% were under GA and 0.98% were under SED. Midazolam was the most commonly used drug for SED. Conclusion: Although gingivitis and pulpitis were the most common reasons for patients with dementia to visit the dentist, GA or SED for patients with dementia was frequently used in oral and maxillofacial or periodontal surgery.

• 한국환경과학회지
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• 제12권10호
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• pp.1109-1116
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• 2003

The genetic algorithm is investigated fer parameters estimation of SED (storage - effective drainage) model from the Wi-stream watershed in Nakdong river basin. In the practical application of model, as a number of watershed parameters do not measure directly, it is desirable to make a good estimation from the known rainfall and runoff data. For the estimation of parameters of the SED model using the genetic algorithm, parameters of Green-Ampt equation(SM, K$\_$s/) for the estimation of an effective rainfall and initial storage(y$\_$in/) used in SED model are obtained a regression equation with 5, 10, 20 days antecedent precipitation. And as a consequence of computation, the parameters were obtained to satisfy the proposed objective function. From the comparison of observed and computed hydrographs, it shows a good agreement in the shape and the rising limb, peak, falling limb of hydrograph, so the SED model using the genetic algorithm shows a suitable model for runoff analysis in river basin.

• 천문학회보
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• 제46권2호
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• pp.51.2-52
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• 2021

Pulsar Wind Nebula(PWN)는 radio부터 TeV band까지 넓은 파장에 걸쳐 복사를 하며 이 복사는 Spectral Energy Distribution(SED)으로 측정된다. 관측된 SED는 두 개의 주요한 bump를 보이는데 low-energy emission bump는 synchrotron radiation에 의해 만들어지고 high-energy emission bump는 inverse Compton scattering에 의해 만들어진다. 대부분 PWN들의 SED는 단일 전자 분포로 설명이 가능하지만 최근 연구 결과에 의하면 Crab nebula, G21.5-0.9 같은 일부 young pulsar wind nebula의 X-ray SED에서 단차나 기울기의 변화 등 단일 전자 분포로 설명하기 어려운 부분이 관측되기도 한다. 이런 PWN에 대하여 우리는 이중 전자 분포를 이용해서 broadband SED가 잘 설명이 되는지 확인하고 이를 통하여 PWN 입자 가속의 특성을 이해해보고자 한다.

• 운동영양학회지
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• 제24권3호
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• pp.1-6
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• 2020

[Purpose] While the anti-obesity effects of exercise and capsiate are well-observed individually, the effect of exercise with capsiate intake has not been systematically explored yet. Therefore, the purpose of this study is to investigate whether the anti-obesity effects of exercise training can be further enhanced by capsiate intake. [Methods] 8-week-old male mice were divided into 3 groups (n = 8 per group): sedentary group (SED; nontrained), exercise-trained group (EXE) and exercise-trained group with 10 mg/kg of capsiate intake (EXE+CAP). All mice were offered high-fat diet and water ad libitum. The mild-intensity treadmill training was conducted 5 times a week for 8 weeks. After 8 weeks, metabolism during exercise and abdominal fat weight were measured. [Results] Body weight and the rate of total abdominal fat were significantly less in EXE+CAP than in SED but not between EXE and SED. The average of respiratory exchange rate during exercise was significantly much lower in EXE+SED (p = 0.003) compared to the difference between EXE and SED (p = 0.025). Likewise, the fat oxidation during exercise was significantly much higher in EXE+SED (p = 0.016) compared to the difference between EXE and SED (p = 0.045). Then, the carbohydrate oxidation during exercise was significantly much lower in EXE+SED (p = 0.003) compared to the difference between EXE and SED (p = 0.028). [Conclusion] In conclusion, the anti-obesity functions of exercise training can be further enhanced by capsiate intake by increasing fat oxidation during exercise. Therefore, we suggest that capsiate could be a candidate supplement which can additively ameliorate obesity when combined with exercise.

• 한국환경과학회지
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• 제23권2호
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• pp.181-192
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• 2014

In Korea, the chemical oxygen demand($COD_{sed}$) in freshwater sediments has been measured by the potassium permanganate method used for marine sediment because of the absence of authorized analytical method. However, this method has not been fully verified for the freshwater sediment. Therefore, the use or modification of the potassium permanganate method or the development of the new $COD_{sed}$ analytical method may be necessary. In this study, two modified $COD_{sed}$ analytical methods such as the modified potassium permanganate method for $COD_{Mn}$ and the modified closed reflux method using potassium dichromate for $COD_{Cr}$ were compared. In the preliminary experiment to estimate the capability of the two oxidants for glucose oxidation, $COD_{Mn}$ and $COD_{Cr}$ were about 70% and 100% of theoretical oxygen demand(ThOD), respectively, indicating that $COD_{Cr}$ was very close to the ThOD. The effective titration ranges in $COD_{Mn}$ and $COD_{Cr}$ were 3.2 to 7.5 mL and 1.0 to 5.0 mL for glucose, 4.3 to 7.5 mL and 1.4 to 4.3 mL for lake sediment, and 2.5 to 5.8 mL and 3.6 to 4.5 mL for river sediment, respectively, within 10% errors. For estimating $COD_{sed}$ recovery(%) in glucose-spiked sediment after aging for 1 day, the mass balances of the $COD_{Mn}$ and $COD_{Cr}$ among glucose, sediments and glucose-spiked sediments were compared. The recoveries of $COD_{Mn}$ and $COD_{Cr}$ were 78% and 78% in glucose-spiked river sediments, 91% and 86% in glucose-spiked lake sediments, 97% and 104% in glucose-spiked sand, and 134% and 107% in glucose-spiked clay, respectively. In conclusion, both methods have high confidence levels in terms of analytical methodology but show significant different $COD_{sed}$ concentrations due to difference in the oxidation powers of the oxidants.

• 천문학회보
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• 제40권2호
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• pp.42.1-42.1
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• 2015

We model the Spectral Energy Distribution (SED) of Class 0 protostar L1527 IRS using a radiative transfer code RADMC-3D. In addition to the photometry data from literatures, we include the Herschel/PACS data which well covers the far-infrared SED peak of L1527 IRS, providing precise constraints to the density structure and other physical properties of its circumstellar envelope. Previously, Tobin et al. (2013) presented a dust continuum modeling results using a rotating and infalling envelope (Terebey and Shu, & Cassen 1984 ; TSC envelope), which originally describes a power-law density profile (${\rho}{\propto}r-{\alpha}$) with the power-law index (${\alpha}$) of 1.5. However, we find that Herschel/PACS data are better fitted with a shallower power-law density profile. This smaller power-law might be attributed to a inner envelope. Thus, we fit the SED of L1527 IRS with a Bonnor-Ebert sphere, which is a combination of the inner flat-topped and the outer power-law (${\alpha}=2$) density profiles. This Bonnor-Ebert sphere is often used to explain the density profile of prestellar cores, which is considered the earliest stages of star formation. The well-fitted SED with a Bonnor-Ebert sphere suggests that L1527 IRS might have collapsed from a Bonnor-Ebert sphere rather than a singular isothermal sphere.

• 생명과학회지
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• 제33권3호
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• pp.242-251
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• 2023

마우스 모델을 사용하여, 8주 케톤체(베타-하이드록시뷰티레이트, β-HB)가 지구성 운동 수행능력과 골격근의 단백질 합성 및 분해에 미치는 영향을 조사하였다. 48마리의 수컷 ICR 마우스(8주령)를 무작위로 4개 그룹으로 나누었다: 비운동 통제군(Sed+Con), 비운동+베타-하이드록시뷰티레이트(Sed+β-HB), 운동 통제군(Exe+Con), 운동+베타-하이드록시뷰티레이트(Exe+β-.HB). β-HB 투여를 위해 β-HB를 PBS (150 mg/mL)에 용해시켜 8주 동안 매일 피하 주사(250 mg/kg)하였다. 운동 훈련을 위해 마우스는 8주 동안 20분 트레드밀 달리기 운동 훈련을 주 5일 수행하였다. 훈련은 10° 경사도에서 10 m/min 속도에서 5 min 동안 실시하고 나서, 매 1 min 마다 1 m/min의 속도를 나머지 15 min 동안 증가시켰다. 8주간의 처치 후, 내장 지방량과 골격근량, 혈액 매개변수, 자가포식 및 단백질 합성 마커가 분석되었다. 데이터는 SPSS 21 프로그램을 사용하여 ANOVA (p<0.05)로 분석되었다. Exe+β-HB 그룹의 혈중 젖산 수치는 모든 3개 그룹(Sed+Con, Sed+β-HB 및 Exe+β-HB)보다 유의하게 낮았다(p<0.05). Sed+Con 및 Exe+Con 그룹에 비해, 8주 Exe+β-HB 처치는 최대 달리기 수행 시간(탈진 시간)을 유의하게 증가시켰다(p<0.05). 8주 β-HB 투여는 마우스의 골격근에서 자가포식 유동과 자가포식 관련 단백질을 유의하게 감소시켰다(p<0.05). 반대로, β-HB와 지구력 운동 훈련의 조합된 처치는 단백질 합성(mTOR 신호 및 번역 속도)을 증가시켰다(p<0.05). 8주간의 β-HB 처치와 지구력 운동 훈련은 마우스 골격근에서 지구력 수행능력 증가, 단백질 합성 증가, 단백질 분해 감소 효과를 보여주었다.

• Steel and Composite Structures
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• 제34권5호
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• pp.671-679
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• 2020

The new energy-based criterion, named Reinforcement Strain Energy Density (ReiSED), is proposed to investigate the fracture behavior of the cracked orthotropic materials in which the crack is embedded in the matrix along the fibers. ReiSED is an extension of the well-known minimum strain energy density criterion. The concept of the reinforced isotropic solid as an advantageous model is the basis of the proposed mixed-mode I/II criterion. This model introduces fibers as reinforcements of the isotropic matrix in orthotropic materials. The effects of fibers are qualified by defining reinforcement coefficients at tension and shear modes. These coefficients, called Reduced Stress (ReSt), provide the possibility of encompassing the fiber fraction in a fracture criterion for the first time. Comparing ReiSED fracture limit curve with experimental data proves the high efficiency of this criterion to predict the fracture behavior of orthotropic materials.

• 천문학회지
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• 제52권5호
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• pp.173-180
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• 2019

We present IR flux density measurements, models of the broadband SED, and results of SED modeling for the Pulsar Wind Nebula (PWN) 3C 58. We find that the Herschel flux density seems to be slightly lower than suggested by interpolation of previous measurements in nearby wavebands, implying that there may be multiple electron populations in 3C 58. We model the SED using a simple stationary one-zone and a more realistic time-evolving multi-zone scenario. The latter includes variations of flow properties in the PWN (injected energy, magnetic field, and bulk speed), radiative energy losses, adiabatic expansion, and diffusion, similar to previous PWN models. From the modeling, we find that a PWN age of 2900-5400 yrs is preferred and that there may be excess emission at ${\sim}10^{11}Hz$. The latter may imply multiple populations of electrons in the PWN.