• Journal of the Korean Wood Science and Technology
• /
• 제34권6호
• /
• pp.1-11
• /
• 2006

접목 과정에서 형성되는 새로운 2차목부의 특징을 광학현미경 및 전자현미경적 차원에서 조사하고자 해송(Pinus thunbergii)을 사용하여 자가접목(autograft)을 실시하였다. 조직학적으로 접목은 괴사층의 형성, 치유조직(callus)의 형성 및 발달, 새로운 형성층의 분화 및 새로운 2차조직의 형성 등 4단계를 거치는 것으로 나타났다. 접목 초기에 형성된 괴사층은 펙틴과 리그닌 성분으로 구성되어 있었으며, 점진적으로 분해되어 대목과 접수가 결합하는 시점에 소멸되었다. 접목 초기에 축적되었던 전분 입자와 지질은 접목이 진행될수록 점진적으로 감소하였으며 세포질 내 소기관, 핵 및 원형질연락사의 변화는 관찰되지 않았다. 유세포로 구성된 치유조직으로부터 접목 15일후부터 새로운 형성층(neocambium)이 발달하였으며, 이곳에서 새로운 2차조직이 형성되기 시작했다. 새로운 2차목부는 형성층에서 기원하는 정상적인 2차목부와는 다른 분화 및 형태를 나타냈다. 새로운 2차목부는 망상 또는 벽공상의 비후에 의한 2차벽을 갖는 가도관 요소에서 기원하였으며, 2차벽의 형성은 비후된 세포벽 사이를 충전시키는 형태로써 진행되었다. 2차벽의 형성은 불균일하고 간헐적이었으며 형성된 2차벽의 두께는 불균일하였다. 새로운 2차목부의 가도관은 S자 또는 소용돌이 모양을 지니는 것으로 수평 방향으로 배열되었다.

• Journal of Applied Biological Chemistry
• /
• 제59권3호
• /
• pp.215-220
• /
• 2016

식물의 이차생장의 결과로 생산되는 바이오매스의 대부분은 식물의 이차세포벽에 축적된다. 식물의 이차세포벽은 크게 셀룰로스, 헤미셀룰로스, 리그닌이라는 3가지 물질로 구성되며 이들 중 페놀성 복합 화학 물질인 리그닌은 셀룰로스나 헤미셀룰로스와 달리 알코올 발효과정의 저해 물질로이다. 따라서, 식물의 이차세포벽 생합성의 과정을 조절함으로 전체 바이오매스의 생산량과 조성을 바이오 에너지 생산을 위해 최적 조건으로 조절함으로써 바이오 에탄올 생산을 최대화할 수 있을 것이다. 본 연구에서는 애기장대 유래 MYB7 유전자를 CaMV35S 프로모터 조절 하에서 과별현되게 한 식물체와 식물의 전사조절에서 하위 특정 유전자의 발현을 저해시키는 것으로 잘 알려진 SRDX 융합 단백질 즉, MYB7-SRDX 과발현체를 제작하여 그 특성을 조사하였다. 그 결과 MYB7 전사조절 인자가 리그닌의 생합성을 억제 조절한다는 결과를 관찰하였다. 이는 MYB7 전사조절인자를 이용하면 바이오 에탄올 발효과정에 저해 물질로 작용하는 리그닌을 경감시킬 수 있는 기초 자료로 사용할 수 있을 것이다.

• 한국추진공학회:학술대회논문집
• /
• 한국추진공학회 2006년도 제27회 추계학술대회논문집
• /
• pp.353-356
• /
• 2006

본 논문에서는 초음속 유동장내에 분사된 이차제트 주변에서의 열전달 현상을 고찰 하였다 초음속 유동장내에 등열유속조건이 적용된 표면을 설치하고, jet to freestream momentum ratio의 변화에 따른 2차분사를 하여, 2차분사 노즐 주변의 표면온도변화를 적외선카메라를 통하여 측정하였으며, 이를 바탕으로 대류열전달계수를 계산하였다.

• 한국분무공학회지
• /
• 제10권4호
• /
• pp.26-31
• /
• 2005

Today, the research to examine a fact that interaction between the air and the fluid free surface affects the steady state flow and air. We proved the interaction between vortex pairs and free surface on each condition that is created by the end of delta wings. Another purpose of this study is to investigate the effect of surface active material which call change the surface tension and we must consider when we refer to turbulent flow on surface tension. Therefore, this research examined the growth process of vortex pairs on condition of clean, contaminated free surface and wall after we made vortex pairs through counter rotating flaps. The results of this study suggest that vortex pairs in clean free surface rise safely but the vortex pairs in contaminated free surface and rigid, no slip is made secondary vortex or rebounding. However the secondary vortex in rigid, no slip is stronger than before. and we can find the vortex shape which roll up more completely. However, these will disappear by the effect of wall.

• 한국윤활학회:학술대회논문집
• /
• 한국윤활학회 2002년도 proceedings of the second asia international conference on tribology
• /
• pp.411-412
• /
• 2002

Piston-cylinder system are widely used in power engineering applications. In reciprocating refrigeration compressors, where extremely low friction losses are required, ringless pistons are being used to diminish the friction between piston rings and cylinder wall. Since the ringless piston has the freedom of lateral motion there is a potential danger that it will occasionally hit the cylinder wall while moving up and down along it's axis. A good design must therefore provide a smooth and stable reciprocating motion of the piston and ensure that the fluid film separating the piston from the cylinder wall is maintained all times. And the compromise between refrigerant gas leakage through the piston-cylinder clearance and the friction losses is required utilizing a dynamic analysis of the secondary motion for the high efficiency compressor. To this end, the computer program is developed for calculating the entire piston trajectory and the lubrication characteristics as functions of crank angle under compressor running conditions. The results explored the effects of some design parameters and operating conditions on the stability of the piston, the oil leakage, and friction losses.

• Journal of Plant Biology
• /
• 제29권3호
• /
• pp.185-196
• /
• 1986

Anatomical characteristics of the secondary xylem in the roots and the stems of genus Pinus including nine species growing naturally in Korea have been studied to elucidate the structural differences between two organs. The results, comparing the root woods with the stem woods, are as follows; The transition from the early-wood to the late-wood occurs more gradually in the root woods. In the root woods, the bordered pits on the radial wall of tracheid are frequent in the two rows. The spiral thickening on the radial and tangential wall of the tracheid can be seen in the only stem woods of four species such as P. koraiensis, P. rigida, P. rigitaeda and P. banksiana. In the majority of the species studied, the length of the tracheid is longer in the root woods, but in some species such as P. strobus, P. densiflora, P. rigida and P. sylvestris, this length is almost sam ein both organs. The diameter of the tracheid in the root woods is wider. The wall thickness of the tracheid in the early wood is thicker in the root woods, but vice versa in the late woods. More rays per unit area can be seen in the root woods.

• Plant Resources
• /
• 제3권3호
• /
• pp.163-172
• /
• 2000

We compared microstructural features of the ordered cell and disordered leaves in Citrus junos Sieb. by electron microscopy. In the cell of the ordered leaves, many chloroplasts and large vacuoles were particularly observed. Also a lot of vessel, companion cell and big nucleus were presented in vascular bundle regions. The mitochondria and the other organelles were interspersed among the chloroplasts in a thin, peripheral layer of cytoplasm. The chloroplast possessed typical grana and intergranal lamellae, numerous starch grains and a few small osmophilic globules. Besides, microbodies were closely associated with the mitochondria and the chloroplast. The process of the formation of the secondary cell wall from primary cell wall was observed the vessel elements, the tonoplast wall and the secondary cell wall. It was observed that the oil sac with the unique perfume distributed the adjacent cell wall. In the cell of disordered leaves, the all of the organelles were thrust toward the cell wall due to the fusion of vacuoles in the cells. It was observed that a lot of the very small particles spreaded in the cytoplasm. The loss of unique perfume of the leaves was resulted in the destruction of the oil sac. Also, there was not observed grana, lamellae, starch and osmophillic globules in the chloroplast. The small distributed organelles was not observed but the elongation of the cell wall was proceed no longer. Therefore, the plasma membrane diverged from the cell wall. All of organelles in the cell had poor function and deformation. A massive vacuole was fulfilled in single cell and the vacuole contains a lot of large and small particles. The organelles were presented on the side of the cell wall according to the enlargement of vacuole and they were observed to be breakdown.

• Journal of Yeungnam Medical Science
• /
• 제3권1호
• /
• pp.237-241
• /
• 1986

본 교실에서 백서의 대퇴동맥을 이용하여 혈관감자의 회전없이 continuous suture로 미세혈관 문합을 시행해본 결과 다음과 같은 결론을 얻었다. end-to-end anastomosis시 double c1app의 회전이 어려울 정도로 작업공간이 제한된 경우나 end-to-side anastomosis시 secondary Vessel의 길이가 짧아서 후벽을 잘 볼 수 없는 경우에 continuous suture을 이용하면 Back wall을 완전히 노출시킨 상태에서 쉽게 혈관 문합을 시행할 수 있으며 문합시간도 단축시킬 수 있을 것으로 사료된다.

• 한국압력기기공학회 논문집
• /
• 제11권2호
• /
• pp.31-39
• /
• 2015

Pipe wall-thinning by flow-accelerated corrosion (FAC) and various types of erosion is a significant and costly damage phenomenon in secondary piping systems of nuclear power plants (NPPs). Most NPPs have management programs to ensure pipe integrity due to wall-thinning that includes periodic measurements for pipe wall thicknesses using ultrasonic tests (UTs). Nevertheless, thinning evaluations are not easy because the amount of thickness reduction being measured is often quite small compared to the accuracy of the inspection technique. U.S. Electric Power Research Institute (EPRI) had proposed Total Point Method (TPM) as a thinning occurrence evaluation method, which is a very useful method for detecting locally thinned pipes or fittings. However, evaluation engineers have to discern manually the measurement data because there are no numerical algorithm for TPM. In this study, numerical algorithms were developed based on non-parametric and parametric statistical method.

• 보존과학연구
• /
• 통권11호
• /
• pp.87-105
• /
• 1990

Chemical and micro morphological changes of archaeological waterlogged woods from shipwrecked materials in marine situations were investigated which were submerged in seabed for over 900 years. Tested Wood species were Pinusdensiflora, Zelkova serrata, Quercus acutissima and Camellia japonica. The obtained results were summarized as follows; Chemical analysis showed that lignin content was increased, whereas the amout of holocellulose was heavily decreased in the degraded archaeological lwoods(DAW), when compared to the recent woods. The amount of alkalineextractives in the DAW was extremley high. IR spectra showed that disappearance of absortion band at $1,730㎝^-1$ intensity increase at 1,600, 1,500 and $1,270㎝^-1$ and the emergence of single band around $1,050㎝^-1$.Microscopic investigation showed that cell wall of latewood tracheids and fiber in the DAW were severely degraded while, early wood tracheids less degraded. Degradation in the cell wall was mainley occurred in $S_2$layer, while the middle lamella was the least degraded. The micro morphological characteristics of DAW were separation of secondary wall from middle lamella, cavities aligned with micro fibril angle in $S_2$layer and granular appearance of secondary wall by the bacterial attack.